ADVIA® 1800 Chemistry System Reagent Management Online Training

Welcome to the ADVIA® 1800 Chemistry System Reagent Management Online Training course.  In this course you will learn how to manage the reagent inventory window, identify and load the different types of reagents on the system, prepare assay and ancillary reagents, store reagents properly, and assign non-Siemens and non-barcoded reagents, all to support accurate reagent management. Select Next to continue.   Differentiate reagent types List the necessary steps for reagent preparation List the steps for assigning reagents for non Siemens (third party) assays Navigate the Reagent Inventory screen State the reagent storage requirements Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations. You have completed the ADVIA® 1800 Chemistry System Reagent Management Online Training course.  Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz.  Navigate the Reagent Inventory screen Check the Reagent Inventory window to verify that reagents are on board the system and are ready for use. Rectify reagent issues that are backlit yellow or red prior to processing on the system. Differentiate reagent types Assay reagents are used for processing patient samples, calibrators and controls Ancillary reagents are needed on the system to clean the probes, cuvettes, wash stations and mitigate contamination. Assay and ancillary reagents are loaded on the reagent trays. Ancillary reagents are loaded on the Ancillary reagent shelf. Only the reagent trays and the CTT contain ancillary reagents that may require dilution. List the necessary steps for reagent preparation Most assay reagents come ready to use. Few require minor preparation which is listed in the reagent IFU or Information for Use sheet. Ancillary reagents used on the Ancillary reagent tray are ready for use. Dilutions are required of the Cuvette Wash and Probe Wash 3 reagents prior to placing them on the reagent trays (RTT's) and the CTT. State the reagent storage requirements Assay reagents are stored according to the storage information listed in the IFU. Ancillary reagents are stored at room temperature. Be sure to store all reagents at their proper storage specifications. List the steps for assigning reagents for non Siemens (third party) assays Non-barcoded and third party (non-Siemens) reagents need to be assigned on the system so they may be used for processing. Reagent assignment is performed at a supervisor password level. Contact the Technical Solutions Center for log in information. Select Next to continue.   The Reagent Inventory window allows you to verify the following: Reagent presence Test volumes Reagent lot numbers Expiration dates Remaining reagent on-system stability Assay Calibration status Assay QC status To access the Reagent Inventory window, select the Reagent button from the Menu panel then select the Reagent Inventory button. Once open, the reagent inventory window defaults to RTT1 or reagent tray 1. You must select the RTT2 tab to display the inventory for reagent tray 2. Columns backlit either yellow or red in the reagent inventory window require your attention.     Navigate the Reagent Inventory Window Learn how to navigate the Reagent Inventory window. Slide NumberText BlocksCalloutsAudio ScriptImage File1Test Name The Test Name column contains the name of the tests for the reagents loaded on the system. Select Next to continue.Note: If audio does not automatically start, select the play arrow in the top left to begin.The Test name column lists the name of the assays with reagents loaded in the reagent trays. Note that they are arranged alphabetically. Select Next to continue.2Posi.# The Posi. # column displays the position number of the reagent containers on the reagent trays. Reagent tray positions allocated to third party and non barcoded reagents must be assigned in the System Test list screen. Select Next to continue.The position number column displays the position of the reagent containers on Reagent Tray 1 or Reagent Tray 2. There are 56 positions in each of the reagent trays. Note, for non barcoded or third party reagents, the reagent tray positions must be assigned in the System Test List screen. Select Next to continue.3P/B The reagent wedges are identified as either Primary or Backup displayed in the P/B column. The system will only use one reagent container at a time and will use the one designated as the primary reagent container.  Additional reagent containers for the same assay are designated as backup. Select Next to continue.The P/B column identifies the reagent container currently in use as the primary reagent or P and others as backup reagents or B. The system will only use the container designated as a primary reagent. Additional available reagent containers for the same assay are designated as backup. When loading multiple reagent containers for the same assay, the system uses a set of criteria to determine the primary reagent status. Refer to the Online Operator Guide for a list of criteria the system uses to determine primary reagent status. Select Next to continue.4# Tests The # Test column is the number of tests remaining in the reagent container. To ensure availability of reagent for critical samples, it is good laboratory practice to replace the reagent wedge when this column is backlit yellow for an assay. Select Next to continue.The Number Tests column is the number of tests remaining in the reagent container. To ensure availability of reagent for critical samples, it is good laboratory practice to replace the reagent wedge when this column is backlit yellow for an assay. This indicates that less than ten percent of the tests remain. Select Next to continue. 5Lot # The Lot # field displays the reagent lot number which is scanned from the reagent containers. Select Next to continue.The Lot number column displays the reagent lot number. For third party reagents or non barcoded reagents, the lot numbers are entered in the Reagent Container Settings window. Select Next to continue.6Exp Date The Expiration Date is scanned from the reagent container barcode. Select Next to continue.The Expiration Date is scanned from the reagent container barcode. For third party reagents or non-barcoded reagents, the expiration dates are entered in the Reagent Container Settings window. NA is displayed if no expiration date is entered. Select Next to continue.7Cal Interval The Cal Interval Days is the number of days remaining to the end of the calibration interval. Select Next to continue.The Cal Interval Days is the number of days remaining to the end of the calibration interval as indicated in each assay IFU or Instruction for Use. When the Cal Interval reaches zero, the assay line is highlighted in yellow. This indicates that you should recalibrate the assay. Select Next to continue.8Days Remaining The Days Remaining column displays the number of days that the reagent container is stable on the instrument as indicated in the reagent IFU. Select Next to continue.The Days Remaining column displays the number of days remaining to the end of the on-board stability interval indicated in the assay specific Instruction for Use. Note if a reagent container is removed and subsequently reloaded, the Days Remaining box displays remaining days based on the first day which the reagent container was loaded. Select Next to continue.9Total Test Summary Select the Total Test Summary button to view the list of all reagents on-board displaying their maximum total tests counts. For users of ADVIA 1800 version 2 software, the total test counts will display zero tests for assays requiring calibration. Select Next to continue.Select the Total Test Summary button to view a list of all reagents on-board the reagent trays displaying the maximum total tests counts for each. For users of ADVIA 1800 version 2 software, the total test counts will display zero tests for assays requiring calibration. Select Next to continue.10Cal Interval Review Select the Cal Interval Review button to view reagent calibration information sorted by date. Assays with the least number of days remaining for calibration will be listed on top. Select Next to continue.Select the Cal Interval Review button to view reagent calibration information sorted by date. Assays exceeding their calibration interval and those with the least number of days remaining for calibration will be listed on top. Select Next to continue.11Barcode Scan To update the Reagent Inventory screen, perform a barcode scan. This is a 2 step process whereby the barcode readers in the reagent trays scan all positions, and then the reagent probes sense the liquid level in each container to determine volume and tests remaining. The Reagent Confirmation window appears after the scans. This window alerts you of any reagent errors and also allows you to enter misread reagent barcodes.  Select OK to resume the barcode scanning process. Select Next to continue. If reagents are added to or removed from the reagent trays, select the barcode scan button to update the Reagent Inventory screen. This is a 2 step process whereby the barcode readers in the reagent trays scan all positions, and then the reagent probes sense the liquid level in each container to determine volume and tests remaining. Note, the Reagent Confirmation window appears after the systen scans the reagents. This window alerts you of any reagent errors and also allows you to enter misread reagent barcodes by selecting the Timer Off button. Select OK to resume the barcode scanning process. Select Next to continue.12Other Other reagent inventory screen information includes:  Cal status column QC status column Note: Refer to the Online Operator Guide for more information about the Reagent Inventory screen. When complete, select the X in the upper right corner to close the window and continue.Other reagent inventory screen information includes Cal status and QC status Refer to the Online Operator Guide for more information about the Reagent Inventory screen. The ADVIA® 1800 Chemistry system uses two types of reagents: Assay reagents which are used for processing Ancillary reagents which are used for contamination prevention, sample dilution, and maintaining mainintaing the reaction tray or RRV Select Next to continue. Both assay and ancillary reagents are loaded on Reagent Trays. Load assay reagents: Positions 1 through 52 hold assay reagents Positions 1 through 29 are allocated to 40 mL containers Positions 30 through 56 are allocated to 70 mL containers Adaptors must be used in order to load 20 mL containers Note: Reagents are packaged in 20mL, 40 mL, and 70 mL containers. Load ancillary reagents: Ancillary reagents are assigned to positions 53 through 56 Ancillary reagents include 10% Cuvette Wash, Reagent Probe Wash 1, Reagent Probe Wash 2, and deionized water Reagent Probe Wash 1 and Probe Wash 2 are loaded on reagent trays in 70 ml containers and do not require dilution Note: Probe Wash 1 and 2 must be present to process samples from the Ready state. Loading and Unloading Reagent Trays Learn how to load and unload the reagent trays. Slide NumberText BlocksCalloutsAudio ScriptImage File1Loading the Reagent Trays Assay and ancillary reagents may be loaded in the reagent trays when the instrument is the Ready state or after selecting the Reagent Pause button. If Reagent Pause is selected, be sure that the system returns to the "Add Reagents" status before lifting the reagent tray covers. Remove the reagent tray lids, and load the 40mL and 70 mL containers in their respective spaces. Note that the 20mL containers require adapters to be loaded in the reagent trays. Check the label on the reagent container, apply the appropriate test name labels, and load containers labeled R1 in Reagent Tray 1 and R2 in Reagent Tray 2. Remove the reagent caps from the containers before replacing the reagent tray lids. Verify that reagent containers are properly seated in the reagent trays.  Align the reagent tray lids so that they are well fitted on the reagent trays. Failure to remove the caps, to verify proper seating of the containers or to secure the reagent tray lids may cause a probe crash. Note that a barcode scan must be performed after loading or removing reagents. Select Next to continue.      Note: If audio does not automatically start, select the play arrow in the top left to begin.Primary and ancillary reagents may be loaded in the reagent trays when the system is the Ready state or by selecting the Reagent Pause button. This button temporarily stops the reagent trays and allows you to add or remove reagents from the reagent trays. If Reagent Pause is selected, be sure that the system returns to the "Add Reagents" status before lifting the reagent tray covers. Remove the reagent tray lids, and load the 40milliliter and 70 milliliter containers in their respective spaces. Note that the 20milliliter containers require adapters to be loaded. Load reagent containers labeled R1 in Reagent Tray 1 and R2 containers in Reagent Tray 2. Be sure to apply the appropriate test name labels to each container and remove the reagent caps. Verify that reagent containers are properly seated in the reagent trays. Line the reagent tray lids so that they are well fitted on the reagent trays. Failure to remove the caps, to verify proper seating of the containers or to secure the reagent tray lids may cause a probe crash. Note that a reagent barcode scan must be performed to update the Reagent Inventory window. Select Next to continue.2Unloading the Reagent Trays Assay and ancillary reagents may be removed when the instrument is the Ready state or after selecting the Reagent Pause button. If Reagent Pause is selected, wait until the system returns to the "Add Reagents" status before lifting the reagent tray covers. You may remove and add reagents at this time. Be sure to remove both R1 and R2 reagents for assays using two reagents. A barcode scan must be performed after loading or removing reagents. When complete, select the X in the upper right corner to close the window and continue.Assay and ancillary reagents are loaded in the reagent trays and may be removed when the system is the Ready state or by selecting the Reagent Pause button which temporarily stops the reagent trays. For safety reasons, when selecting the Reagent Pause button, wait until the system is at the ADD Reagents status before lifting the reagent tray covers. Corresponding reagents for assays that use R1 and R2 reagents must be added and removed simultaneously. Note that after adding or removing reagents, you must perform a barcode scan in order to update the Reagent Inventory window. The prostate is a male sex gland properly known as the prostate gland. It is located immediately under the urinary bladder and surrounds the urethra. It is about the size of a chestnut and covered by a casing, the capsule. Some of the semen is produced in the prostate and its function is regulated by the hormone testosterone. The Ancillary Reagent Shelf stores only ancillary reagents. They include: ISE buffer Incubation bath oil 0.9% Isotonic saline (not a Siemens product) Cuvette Wash Cuvette Conditioner These reagents are necessary to: Clean the probes Clean the wash stations Clean the cuvettes Dilute samples Maintain the level of the reaction bath Load and Remove Ancillary Reagents Learn how to load and remove ancillary reagents. Slide NumberText BlocksCalloutsAudio ScriptImage File1Loading Ancillary Reagents To load the ancillary reagents: Verify that the system is in the Ready state. Unscrew the bottle filter cap. Remove the straw from the bottle. Insert a funnel on top of the bottle. Pour the appropriate ancillary reagent in the bottle. Replace the straw. Replace the bottle filter cap. Perform a Prime 2 after adding to the reagent bottles residing on the Ancillary reagent shelf. Perform ISE bufferprime after changing the ISE buffer bottle. Select Next to continue. Note: If audio does not automatically start, select the play arrow in the top left to begin.To load the ancillary reagents, verify that the system is in the Ready state. Unscrew the bottle filter cap. Remove the straw from the bottle and insert a funnel on top of the bottle. Check labels on the bottles, and pour the appropriate ancillary reagent in the bottles. Replace the straw and replace the bottle filter cap. Note, the ISE buffer should not be topped off but replaced. Perform a Prime 2 for all reagents residing on the Ancillary reagent shelf (except the ISE buffer). Perform an ISE bufferprime after changing the ISE buffer. Note, ISE calibration is required when using a new ISE buffer lot. Select Next to continue.2Removing Ancillary Reagents The ISE buffer bottle is the only ancillary reagent on the Ancillary reagent tray that is removed when empty. To remove and replace the ISE buffer when empty: Unscrew the screw top and remove the sensor. Replace with a new ISE buffer bottle. Replace the sensor and screw top. Perform ISE bufferprime. Note: ISE calibration is required if a new lot of buffer is placed on the system. Follow the instrument maintenance guidelines to remove and replace the other ancillary reagent bottles residing on the Ancillary reagent tray.When complete, select the X in the upper right corner to close the window and continue.The ISE buffer is the only reagent on the Ancillary reagent shelf that is removed and replaced when empty. The other ancillary reagents bottles are removed and cleaned periodically. Refer to the Online Operator guide for scheduled maintenance procedures. To remove the ISE bottle, unscrew the screw top and remove the sensor. Replace with a new ISE buffer bottle. Replace the sensor and screw top. Perform a bufferprime. Note, ISE calibration is required if a new lot of buffer is placed on the system. Ancillary reagents also reside on the Calibrator/Control Tray (CTT). These include 10% cuvette wash solution, 5% probe wash 3 solution, ISE detergent, and deionized water. These solutions are used during the Shutdown Wash maintenance procedure and are loaded on the CTT in tubes and sample cups which need to be replenished daily. There may also be some assay-specific ancillary reagents such as the HemoglobinA1c_3 Denaturant reagent loaded on the CTT. Refer to the assay IFU for more information. Select Next to continue . There are different reagent preparation requirements for the various reagents used on the system. Reagent Preparation Learn more about reagent preparation requirements. Tab TitleTextAssay Reagents For preparation of assay reagents, refer to each reagent  Instruction For Use sheet or IFU.    Ancillary Tray Reagents The ancillary reagents residing on the Ancillary Reagent Tray do not require preparation.  Other Ancillary Reagents Some ancillary reagents loaded on the reagent trays and the CTT need to be diluted prior to use. These consist of the Cuvette Wash and Reagent Probe Wash 3 solutions. The dilutions needed are as follows: 10% Cuvette Wash in de-ionized water 5% Probe Wash 3 in de-ionized water The 5% Reagent Probe Wash 3 solution may be substituted for the 10% Cuvette Wash solution during the Shutdown Wash maintenance procedure. The lamp coolant additive used in the lamp coolant reservoir is the only other ancillary solution that requires a dilution prior to use.  For more information, refer to the Maintenance section of the Online Operator guide.   When complete, select the X in the upper-right corner to close the window and continue. There are different storage requirements for assay reagents. Some are stored at room temperature, refrigerated, and some are light sensitive. Refer to the Information for Use sheet (IFU) for proper storage requirements. Note: Ancillary reagents and their corresponding working solutions are stored at room temperature. For assay specific ancillary reagents, refer to the Instruction for Use sheet for proper storage requirements. Select Next to continue.   The ADVIA® 1800 Chemistry System is an open system which allows you to process assays from other vendors. If using a non Siemens (third party) assay on the system, you will need to contact the assay vendor for assay specific information. Note:  Siemens does not provide support for third party or non Siemens assays. Normally, after performing a barcode scan, validated Siemens reagents are recognized on the reagent trays.  However, in the case of third party and non barcoded reagents, they must be manually assigned to the reagent trays for effective reagent management.   Assigning a Third Party Reagent Learn how to assign a third party reagent or a non barcoded reagent on the system. 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