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ADVIA® 2120i Hematology System Calibration Online Training

Perform the calibration procedure and review and accept calibration data. This clinical laboratory training qualifies for continuing education units (CEU).

Welcome to ADVIA® 2120i Hematology System Calibration Online Training course. This course will cover the step-by-step process for performing calibration. It also contains information regarding when a calibration should be performed, the materials required, and what to check before performing calibration. Select Next to continue.   Access and navigate through the Calibration Wizard Determine how to handle calibration interruptions and failures Determine the calibration options and materials required Determine when a calibration is required Identify the items that need to be checked before calibrating Review and accept calibration data Upon successful completion of this course, you will be able to: Select Next to continue. Felicitaciones. Ha completado el curso de capacitación en línea de la calibración del analizador químico de orina automatizado CLINITEK Novus™ A continuación se enumeran los puntos clave que se han presentado. Tómese el tiempo para revisar el material antes de proceder al cuestionario final. Congratulations.  You have completed the ADVIA® 2120i System Calibration online training course.  Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz.      Determine when a calibration is required Calibration is done to eliminate system bias Calibrate if there is a significant shift in control values after        Installing reagents with a different lot number        Replacing a critical hydraulic or optical component If commercial controls or moving averages are out of range and you have verified that the out-of-control condition is not instrument related As required on a regular basis as determined by your laboratory procedures or inspecting agency Identify necessary checks before calibrating   Perform needed maintenance   Check Pressure and Vacuum readings   Inspect Hydraulics   Verify optics   Check reagents to make sure there is sufficient volume to complete calibration Determine the calibration options and materials required   Calibrate with a Commercial Calibrator or Whole Blood   Choose the primary sampler type   Select the parameters to calibrate   Calibrate with or without the Wizard Access and navigate through the Calibration Wizard   Calibrate with the Wizard to navigate easily through the calibration steps   Use Next to move to next step, Back to go back to previous screen, and Exit to exit the calibration procedure   Review and accept calibration data   Print calibration data before accepting the calibration since data will no longer be available after calibration is accepted   Eliminate any outliers if precision fails  Determine how to handle calibration interruptions and failures If a Probe Clog message is received while you are running the Autosampler to calibrate the system, you must exit the Calibration tab, correct the probe clog, and then resume the calibration procedure. The message typically appears due to a loose sample line fitting on the Autosampler.  When exiting calibration before completing the procedure remember to follow the prompts upon returning to resume  A sufficient number of data points is required to pass calibration, reinstate good data points  The calibration factor must be in the range of 0.5-1.5 Select Next to continue. Calibration is the process used to eliminate system bias in the analytical process with standard reference methods or by using another system, and it is done to maintain instrument accuracy. For example, in this scenario you have replaced the 50 μL Syringe Plunger and thoroughly cleaned the plunger and barrel. In addition to checking for leaks and air bubbles, you have tightened all fittings. You begin to run samples and you notice that the RBC results are running higher than expected. The control values have also verified that the results are running higher. You ask yourself, "What could be the issue?" Because you replaced a major component on the system, you realize that you may need to calibrate the analyzer so that results are reported accurately.  After making sure the maintenance procedure was performed correctly you prepare the system for calibration following the procedure in the Operator’s Guide. Select Next to continue.   Welcome to the ADVIA QC version 2.5 Online Training Course.  ADVIA QC provides both real-time and long-term evaluation of analyzer and method performance.  This course covers the basic features of ADVIA QC version 2.5, including how to review QC data and investigating QC that’s out of range. Select Next to continue. A calibration factor is associated with a parameter. It is important to identify the parameters that are affected by performing or not performing a calibration. The calibratable parameters are the following: Calibratable Parameters WBCB RTC RBC WBCP MCVg RBC CHCMg Hgb %Retic MCV   CHCM   Plt   MPV     It is also important to identify the relationship between calibration factors. Note: Changing certain calibration factors can adversely affect the results reported by the system for a related parameter. For example, if you change the RBC calibration factor, you should recalibrate PLT and RTC RBC. Because of these relationships, you should avoid manually changing calibration factors.   Calibration Factor Relationships Learn more about calibration factor relationships. Base ImageHotspotsText BlocksImage File The Perox WBC (WBCP) is calibrated in all three sampling modes (autosampler, manual closed tube and manual open tube) using the SETpoint calibrator material. The Baso WBC count is calibrated in all sampling modes (autosampler, manual closed tube, and manual open tube) using the SETpoint calibrator as the reference for each mode. RBC, HGB, MCV, CHCM, and PLT are calibrated against the calibrator. Retic RBC, MCVg, and CHCMg are calibrated by running a whole blood sample using the results obtained in the RBC channel for RBC, MCV and CHCM as the reference for the Retic RBC, MCVg and CHCMg calibration factors. The %Retic cal factor is 1.00 unless this instrument is calibrated against another instrument. The MPV cal factor is displayed as a default factor of 1.00. Commercial and Whole Blood Calibration Learn about Commercial and Whole Blood Calibration. Tab TitleTextCommercial Calibration The relationship between calibration factors is displayed here. It is important when reviewing data to realize the affect the parameters listed on the left will have on those listed on the right. For example, if RBC fails calibration so will PLT and RTC RBC. Calibration Factor Related Parameters RBC                                         PLT, RTC RBC   MCV CHCM, MCVg, CHCMg CHCM CHCMg MCVg CHCMg      Whole Blood Calibration When calibrating with SETpoint the WBC parameters are calibrated using the reference listed for each mode on the package insert. During whole blood calibration the Baso WBC is calibrated on the autosampler which will be used as the reference for WBCB in the manual closed and open modes and also the WBCP on the Autosampler. WBCP in the manual open and closed modes are calibrated using the WBCP Autosampler as the reference. Calibration Factor Related Parameters Baso WBC (Autosampler) Baso WBC (MCTS), Baso WBC (MOTS), Perox WBC (Autosampler) Perox WBC (Autosampler) Perox WBC (MCTS), Perox WBC (MOTS)  RBC PLT, RTC RBC MCV CHCM, MCVg, CHCMg CHCM  CHCM MCVg CHCMg When complete, select the X in the upper-right corner to close the window and continue.    어세이 에러는 테스트가 자동으로 완료되는 것을 막는 연결된 장비에서의 에러입니다. 예를 들어, 장비에서 처리 문제를 겪고 VersaCell X3 시스템이 연결된 장비에서 세부 에러 정보를 받지 않으면 인벤토리 소진 에러가 발생됩니다. 어세이 에러의 세부 사항을 보려면 Samples 화면 또는 Instruments 화면에 액세스 합니다. Samples 화면에서 어세이 에러가 있는 샘플은 노란색으로 표시됩니다. . Instruments 화면에서 어세이 에러는 하나 이상의 연관된 장비 에러를 발생시킬 수 있습니다. 오더가 장비의 용량을 초과하거나 필요한 정보가 부족할 때 영구적인 오더 에러가 발생합니다. 어세이 에러와 영구 오더 에러 해결에 관해 배우려면 링크를 선택하세요. Calibration is performed to eliminate systematic bias with standard reference methods or another system.  Calibration should be performed on an as needed basis and in each of the following cases: Upon initial installation of the system If there is a significant shift in control values after: Installing reagents with a different lot number Replacing a critical hydraulic or optical component If commercial controls or moving averages are out of range and you have verified that the out-of-control condition is not instrument related As required on a regular basis as determined by your laboratory procedures or inspecting agency Select Next to continue.   There are several items to check and perform prior to running a system calibration. These items are done to minimize interruptions in the calibration process.   What to Check and Perform Before Calibrating Learn more about what to check and perform before calibrating. Checklist TitleChecklist TypeChecklist ContentPerform MaintenanceHTML Select each checkbox to learn more about what to check and perform before calibrating. All instrument maintenance should be up-to-date prior to calibrating the analyzer.   Perform the "Cleaning the Shear Valve and Aspiration Pathways" procedure as described under Weekly Routine Maintenance in the Operator's Guide.  Check Pressure and VacuumHTML Check the pressure and vacuum readings on the Analyzer Status tab of the Utilities menu. Note: The system must be in the Ready to Run mode to perform this check.  Inspect HydraulicsHTML Visually inspect the hydraulics during operation to ensure that they are functioning properly by performing a "refresh" to run background counts or by running a sample.  Check for proper fluid flow in the syringes and UFC during sample processing. Make sure that there are no leaks or air bubbles present. Also check to be sure that syringe plunger maintenance is up-to date.  Verify OpticsHTML Verify that the optical system is functioning properly by reviewing the information displayed on a Run Screen. The cell populations of a normal sample should have the proper shape and be positioned correctly on the cytograms. This information along with QC can be used to verify the gains and alignment of the optical channels. Calibration should only be performed if the gains and alignments of all the optical channels have been verified that they are within specification.  Check Reagent VolumesHTML Check the Startup Screen to verify that there is sufficient reagent to complete the calibration. The amount needed will vary depending upon the type of calibration you are running. If there is not enough reagent, be sure to replace it prior to starting the calibration process. It is important that you do not change the reagent lot number during the calibration.  Verify Stop CriteriaHTML Make sure the Aspiration Failures Stop criterion is set to 1 at the Alarm / Stop Criteria window. This is done so that if a Probe Clog error occurs while you are using the Autosampler to calibrate the system, the Autosampler stops aspirating. When this occurs, you must exit the Calibration tab, correct the probe clog, and then resume calibration. It is important that you follow the prompts displayed when resuming calibration because partial calibration data is available and the software allows you to continue from where calibration was paused. If you do not select "Resume" when prompted, you will have to go through all of the calibration steps from the beginning of the procedure including rerunning the samples that were already processed. Turn On/Off Run Screen printoutHTML If you want the Run Screen to print for every aspiration during the calibration process, select Auto Print Run Screen in System Options. You can select Print None if you do not want a printout for every aspiration. When complete, select the X in the upper-right corner to close the window and continue.   Calibration is located under the Procedures menu. There are several options or combination of options for calibrating the system.  The choices that need to be made when calibrating are listed below: Calibrating with or without the Wizard Commercial Calibration Procedure or Whole Blood Calibration Procedure Calibrating with or without the Autosampler It is recommended that you select the Calibrating with the Wizard option because it facilitates navigation through the entire process. Use of the Wizard is covered later in the course.   Options for Calibrating Learn more about the listed options. Tab TitleTextWith or without the Wizard Calibrating with the Wizard steps a user through the Calibration procedure from start to finish with prompts along the way. A Next button is selected on each page to proceed to the next step in the process. The side buttons are actually grayed out and cannot be selected. Calibrating without the Wizard is a manual mode of operation where the user must step through each of the side buttons listed. There is no Next button to guide you to the next step. Commercial or Whole Blood Commercial calibrator such as SETpoint: Contains a scannable bar code sheet for entering reference values Reference values are scanned into the Cal Definition screen A single reference value is used for each WBC count in each sampling mode Whole Blood: Whole blood samples are run on another system to obtain reference values Reference values are manually typed in the Cal Definition screen A reference value is entered for Baso WBC Autosampler and then Perox WBC Autosampler is cross calibrated with Baso WBC Autosampler BASO WBC MCTS and MOTS are cross calibrated with BASO WBC Autosampler, Perox WBC MCTS and MOTS are cross calibrated with Perox WBC Autosampler With or without the Autosampler The sampling mode used most often is defined as the primary sampler type. Most operators will define the Autosampler as the primary sampler type. If no Autosampler is available then MCTS or MOTS would be selected as the primary sampler type. When complete, select the X in the upper-right corner to close the window and continue. ADVIA® SETpoint Calibrator and/or whole blood samples are used to calibrate all parameters. The following are the materials required during the calibration procedure: ADVIA SETpoint calibrator:    Comes with two 6.1 ml vials of calibrator material per package Fresh whole blood:    Used to calibrate the RETIC parameters when CHr or other Retic indices    are monitored    Used to calibrate %RETIC    Used instead of a commercial calibrator to calibrate the system Note: When using whole blood in place of the commercial calibrator, it needs to be run on a currently validated reference analyzer to determine the target values of the sample. To view the differences about the samples used during calibration, select the link below.   Calibration Materials Learn about calibration materials. Tab TitleTextADVIA SETpoint Calibrator Using ADVIA SETpoint Calibrator Refer to the product insert for complete details concerning description, handling, and System Specific Values (SSV's). Follow the steps below for using this calibrator. Allow calibrator product to equilibrate to room temperature for approximately 15 minutes prior to testing. Manually mix vials by inversion until the RBC's are completely resuspended. Return the calibrator material to the refrigerator immediately after use to maintain its stability.    Whole Blood Requirements for Whole Blood Calibrators: Specimens must be less than 8 hours old Specimens should produce values within the expected clinical ranges Note: While some variation from these ranges is allowed, specimens that produce test results that exceed the reportable test ranges or produce morphology flags are not acceptable. When complete, select the X in the upper-right corner to close the window and continue.    As mentioned previously, Calibration can be performed by using the Calibration Wizard located in the system software. The Wizard is a tool to guide you, using step-by-step navigation assistance, through the calibration procedure. The Wizard eliminates off-line calculations as it will calculate the new calibration factor for you. Before beginning the calibration process, remember to: Verify the system performance Confirm that the system maintenance is up-to-date In this portion of the course we will provide you with a demonstration on how to use the Wizard and then you will have the opportunity to practice using it through a simulation.   Calibration Wizard View how the Calibration Wizard is used. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90004513/sim_ADVIA2120i_Calibration_YourTurn_800x600_9/sim_ADVIA2120i_Calibration_YourTurn_800x600.swfHTML5 File:/content/generator/Course_90004513/sim_ADVIA2120i_Calibration_YourTurn_800x600_9/index.htmlPDF File: You have just learned how to perform a Calibration, now it is your turn to practice. Select the link below to begin.   Perform a Calibration Practice how to perform a Calibration. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90004513/sim_ADVIA2120i_CalibrationPracticeYT_9/sim_ADVIA2120i_CalibrationPracticeYT_9.swfHTML5 File:/content/generator/Course_90004513/sim_ADVIA2120i_CalibrationPracticeYT_9/index.htmlPDF File: It is recommended that you use the Wizard to perform calibration. If you choose not to use the Wizard tool, the procedure that you will follow is very similar. Below are the major differences. To obtain additional information, refer to the Operator's Guide. As previously described, when using the Wizard after completing each step you selected Next. Without the Wizard each step in the process is accessed by selecting the side buttons in the Calibration screen.   Calibrating Without Using the Wizard Learn more about Calibrating without the Wizard. Checklist TitleChecklist TypeChecklist ContentCalibration IntroductionHTML Select each checkbox to learn more about calibrating without using the wizard. The software screen necessary to perform Calibration with or without the Wizard is located under the Procedures menu. To calibrate without the Wizard select the Cal Definition button on the left. Do not select the Calibration Wizard button. The side button on the left will be selected as each step is completed. Note: You can select Exit to leave the calibration at any time. If you exit before completing the calibration process, the system saves the data for the samples you have already processed.  When you next enter the calibration tab, the system asks you if you want to use or discard the data. If the calibration procedure is stopped before being completed, you must select Exit, eject the sample rack and then return to the Calibration procedure following the instructions that are displayed. Modify Calibration DefinitionHTML Select the primary sampler type and type of calibration. In addition, the option to change the Number of Aspirations and the Number of Whole Blood Samples is available. Remember that a minimum of eight aspirations is still required to pass calibration. The number of whole blood samples number is only needed for the Whole Blood Calibration procedure. The OK button is selected before navigating to the next screen.  Select Calibration Reference DataHTML Select the Calibration Reference Data button to enter the reference values either by scanning the SETpoint barcoded sheet for a Commercial Calibration or manually entering in the reference values if the Whole Blood Calibration was selected in the Cal Definition screen. The OK button is selected before navigating to the next screen.  Run CalibratorHTML Select Run Calibrator and follow the steps for loading the samples and running them on the Autosampler and then in the Manual Closed Tube and Manual Open Tube sampling modes.  Review Calibration DataHTML Select Cal Data Review to view and print individual aspiration data. There are three sets of parameters to view and/or print: WBC RBC/PLT RETIC The screen opens to view WBC data first. You can see both BASO WBC and PEROX WBC data from all three sampling modes. The first data point of the 11 aspirations is automatically omitted as a primer. Omitted data appears in light gray.  The Print Report button can be used at any time before accepting the calibration to obtain a hard copy printout of the data. Once the calibration is accepted the data is cleared from this file. Select the radio button on the right side of the screen to review and print the data for the RBC/Plt and Retic parameters.  Accept CalibrationHTML Select Calibration Acceptance and review the status of each parameter. Select Cal Data Review before selecting Accept Calibration to review the data for each parameter and also to print the data. When you accept the calibration the data is no longer available here for printing. Select Accept Calibration to finalize calibration. The new calibration factors now located in the Cal Factors Log will not be used by the system when analyzing samples. When complete, select the X in the upper-right corner to close the window and continue.    Our urine can be viewed as an indicator of what’s going on inside our bodies. Apart from blood testing, conventional urinalysis is the most important analytical method in terms of identifying diseases of the urinary tract, bladder or kidneys as well as metabolic diseases such as diabetes. This simple examination can determine a number of anomalies. Test strips with certain reagents can simply be dipped into urine in order to check for the presence of sugar or proteins that shouldn't be there or to determine the pH value of the urine. Advanced chemical analyses make it possible to measure the “quantity” of these components, which in principle belong to the field of clinical chemistry.  A test strip determines whether or not glucose levels in a urine sample are elevated. Under normal circumstances urine contains very little glucose. In such cases a test strip will yield a “negative” result. Diabetes is the most common cause of increased glucose excretion in urine. However, other kidney diseases may also cause increased sugar levels in urine.   Calibration errors may occur during the calibration run or during review of the calibration data. Common errors include: Probe Clog Precision Insufficient number of data points Cal factor range     Handling Errors During Calibration Learn about calibration errors and how to handle them. Tab TitleTextProbe Clog If a Probe Clog message is received while you are running the Autosampler to calibrate the system, you must exit the Calibration tab, correct the probe clog, and then resume the calibration procedure. The message typically appears due to a loose sample line fitting on the Autosampler. Pay attention to the prompts when re-entering the Wizard as it will ask if you want to resume the Calibration that was started. Select Yes to resume.    Precision Precision Specification Limits If the calculated standard deviation (SD) for the calibration result is greater than the specified limit, the parameter fails calibration. If your system is using a unit set other than the default, the SD limit will differ accordingly. Evaluate the data to determine if excluding an outlier improves precision.   Parameter SD Limit WBCB 0.20 x 103/μL WBCP 0.30 x 103/μL RBC 0.06 x 106/μL Hgb 0.20 g/dL Plt 8.8 x 103/μL MCV 0.7 fL CHCM 0.25 g/dL RTC RBC 0.06 x 106/μL MCVg 0.70 fL CHCMg 0.25 g/dL Note: The system calculates the SD beyond two decimal places. Consequently, a result that displays to be equal to the limit can fail because the actual value is greater than the limit. Example: A displayed RBC result of 0.06 could fail because the actual calculated value is 0.0625, thus it is greater than the limit. If your system is using a unit set other than the default, the SD limit will differ accordingly. Insufficient Number of Data Points There are a minimum number of data points required for calibration. Data points excluded by the software can be reinstated. Example: The first row of data for BASO WBC(AS) is grayed out(omitted). Click the gray box in the Apply column to reinstate the data.    Cal Factor Range Acceptable Cal Factors must be between 0.05 and 1.50. If the Cal Factor is out of range, check the reference value screen to make sure the reference value is correct. When complete, select the X in the upper-right corner to close the window and continue.

  • 120
  • 2120
  • 2120i
  • wizard
  • material
  • failures
  • interruptions
  • factors
  • parameters
  • ADVIA SETpoint
  • handling errors
  • OLT