PEPconnect

Cleaning Centering Collars Video- Manual and Autosampler

This video provides instructions for cleaning the centering collar: Manual and Autosampler on the ADVIA® 2120i System. 

To clean the manual, close Tube sampler, centering color, first turned the instrument off to prevent personal injury, open the lower door of the analyzer. Pull the manual centering color up to remove the centering color from the instrument. Remove the tubing from the port to avoid personal injury and exposure to a potential biohazard, you must cover the needle with the red needle cover immediately after you remove the centering color. Be careful not to bend the needle as you slip the cover over it. Place the centering color in a beaker filled with a 25% solution of household bleach and water and let it soak for five minutes. Using a cotton swab, scrub off any remaining residue, then rinse with water. Use a stilet or a piece of thin wire to clean the ports and center bore on the centering color. Attach a piece of .030 inch inside diameter tubing to a syringe, then flush the port of the manual closed tube sampler centering color. Rinse the center in color with DI water. Reconnect the tube marked V 46 to the centering color on the manual closed tube sampler. Remove the needle cover and carefully replace the color over the needle. Reinstall the centering color. The instrument must remain off to clean the autosampler centering color. Remove the sample lines from the bottom of the needle bases. Note single aspirate assemblies have one centering color. Loosen the thumbscrews and tilt the Autosampler aspirator assembly forward. On each aspirator, pull up the spring loaded Nob, turn it 1/4 turn, then remove the centering color by pulling it up and out. If the centering color is stuck in place, squirt a little DI water over the color to loosen it. Note the dual Autosampler has two centering colors, one for analyzer sampling and one for optional auto slide sampling, both centering colors. Use the same cleaning method to avoid personal injury and exposure to a potential biohazard. You must cover the needle with the Red needle cover. Immediately after you remove the centering color, be careful not to bend the needle as you slip the cover over it. Remove the three tygon tubes from each centering color. Place the centering color in a beaker filled with a 25% solution of household bleach and water and let it soak for five minutes. Using a cotton swab, scrub off any remaining residue. Use a stilet or a piece of thin wire to clean the ports and center bore on the autosampler centering colors. Attach a piece of .030 inch inside diameter tubing to a syringe filled with DI water, then flush through the three ports of the centering color. Rinse the centering color with DI water to remove any of the bleach solution left behind. To prevent Autosampler centering color lockups, applied Parker, Super O Lube or an equivalent lubricant to the barrel part of the centering color. Do not get lubricant near the needle port or the needle base. Re Connect all of the tubes except the sample line on the autosampler to the centering color. Ensure that the numbered tygon tubes are put on the correct port V 43 V 44 V 45. If an auto slide centering collar is on the instrument, the tygon tubing with the Y connector should be on the two ports to the far left. Remove the needle cover and carefully replace the collar over the needle. On the autosampler centering color, be sure to turn the spring loaded Nob back to its original position on the Autosampler repositioned the Aspirator assembly. Make sure that it drops firmly in place over the guide pins. The guide pins are located top left and top right. Then reconnect the sample line to the base of the centering color. Caution after repositioning the aspirator assembly finger, tighten the thumbscrews, being careful that they are not cross threaded over tightening the screws can warp the baseplate, which will cause misalignment of the sampler miss threading. The thumbscrews can cause needle damage. Close the analyzer cover. Turn on the analyzer power. Check Saline background counts and run whole blood primers to verify system performance.

Cleaning the Cleaning Centering Collars - Centering Collars Manual and Autosampler Autosampler Manual Closed tube 3,-

  • 120
  • 2120
  • closed tube
  • turn off
  • open lower door
  • pull collar up
  • remove tubing
  • cover needle
  • red needle cover
  • bleach
  • soak
  • 5 minutes
  • scrub
  • rinse with water
  • stylet
  • bore
  • syringe
  • v46
  • reinstall
  • remove sample lines
  • aspirator assembly
  • spring loaded knob
  • DI water
  • DEI water
  • remove tygon tubes
  • beaker
  • 25% bleach solution
  • cotton swab
  • thin wire
  • lockups
  • lubricant
  • spring loaded knob
  • guide pins
  • reconnect
  • finger tighten
  • overtighten
  • needle damage
  • close cover
  • close door
  • turn on power
  • whole blood primers
  • check saline background counts
  • controls
  • leaks
  • air bubbles
  • retighten
  • hydraulic lines