ADVIA® 2120i Hematology System How the System Works Online Training

Access and use the Hydraulic Flow diagram Adjust pressures and vacuum Describe shuttling and counting Identify sample and reagent pathways to reaction chambers Identify sample selectivity pathways Identify the components of the UFC and their function Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations. You have completed the How the System Works online training course. Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz. Identify the components of the UFC and their function: The Unified Fluidics Circuit (UFC) , contains the Baso, RBC platelet, Retic and Hemoglobin reaction chambers. The Perox reaction chamber is also located here, although it  is on the outside of the UFC. The vacuum shuttle chambers, the liquid separator chamber and the equilibration chamber are also located in the UFC. Access and use the Hydraulic Flow diagram: The hydraulics diagram is found in the Operator's Guide. Use the Hydraulic Flow Diagram as a reference to view how the fluids flow throughout the system. All of the valves are noted, the various chambers are depicted, and all of the reagent lines and waste lines are color coded. The syringes, aspiration modes and reagent pumps are also depicted.   Identify sample selectivity pathways: The A2120i has three sample selectivity pathways. They are: CBC pathway CBC/diff pathway CBC/diff/retic pathway Identify sample and reagent pathways to reaction chambers: The 175µL of sample, passes through the selector valve which clot filter no matter which mode of aspiration is used. Next, it makes contact with conductivity detector one and then enters the sample shear valve. Here it is aliquotted for the desired selectivities. The sample then contacts conductivity detector two. At this point the sample shear valve turns to the dispense position and the reagent pumps push the sample and reagent to their respective chambers.  The sample and reagents mix and the reaction occurs.   Describe shuttling and counting: Each of the reactions, excluding hemoglobin, is shuttled from the reaction chamber to the syringe for delivery and reading in the flowcell. Adjust pressures and vacuum: The ADVIA 2120i works with hydraulics and pneumatics. Vacuums and pressures are constantly monitored and can be viewed on the Analyzer Status screen. The vacuum and pressures are adjustable to ensure proper delivery and analysis of samples. Select Next to continue.     The ADVIA® 2120i Hematology System utilizes the hydraulic and pneumatic system to deliver the sample and reagent into the flowcell for analysis. The Fluidics Process Learn about the fluidics process on the ADVIA 2120i system.   Select the play arrow to begin the animation. When complete, select the X in the upper-right corner to close the window and continue.     The ADVIA® 2120i Hematology System opens and closes valves at specified times to move fluids through the system. The two pathways the system utilizes are: Hydraulics Pneumatics Hydraulics and Pneumatics Learn about hydraulics and pneumatic pathways. Tab TitleTextHydraulics Hydraulics is the flow of fluids throughout the ADVIA 2120i system. The flow and direction of fluid is controlled by valves and syringe pumps.    Pneumatics Pneumatics is the use of pressures and vacuum in the ADVIA 2120i system. Vacuum and pressure are supplied by a compressor located in a drawer at the back of the instrument. When complete, select the X in the upper-right corner to close the window and continue.      The Unified Fluidics Circuit, or UFC, is the "heart" of the ADVIA® 2120i Hematology System. Made up of eight acrylic plates bonded together Contains hydraulic and pneumatic pathways Delivers sample and reagents into 5 reaction chambers for analysis The shear valve aliquots the sample to the correct location UFC Components Learn about the UFC components. Base ImageHotspotsText BlocksImage FileShear Valve The shear valve, located on the front of the UFC, is made up of two ceramic disks.  The rear disk is stationary. The front disk rotates to "shear" or divide the sample into appropriate aliquots for analysis.The front disk also rotates to allow aspiration for direct cytometry.Sample Input Fitting The sample input fitting connects the sample line. Sample travels from the selector valve to the UFC through the sample line. Hemoglobin Assembly The hemoglobin colorimeter assembly straddles the UFC block at the top. The colorimeter contains a light source set at 564 nm or 546 nm, according to the hemoglobin method selected. The hemoglobin reaction chamber is built into the UFC. Here the sample and reagent mix and then the colorimeter assembly reads the reaction in the hemoglobin reaction chamber. Baso reaction chamber The baso reaction chamber is temperature controlled, 32º C to 34º C. The mixture of the sample and Baso reagent is heated to achieve the desired cytochemical reaction.Retic reaction chamber The retic reaction chamber serves as a container where the Retic reagent and sample are mixed for the desired cytochemical reaction.RBC vacuum shuttle chamber The RBC vacuum shuttle chamber is used in the shuttling of the Baso, RBC/Plt and Retic reactions to the RBC flowcell.Perox reaction chamber The perox reaction chamber is the only chamber mounted on the outside of the UFC. The reaction occurs at temperatures between 58°C to 72.1°C   Perox Vacuum shuttle chamber The perox vacuum shuttle chamber is used to shuttle the Perox reaction to the perox flowcell.Dome valves Dome valves are located in the pathways throughout the UFC and control the fluid flow and vacuum. Coupler plate The coupler plate has front fittings for the rinse delivery and drying of needles and sample probe and the delivery of defoamer. Liquid separator chamber UFC vacuum is monitored at the liquid separator chamber.Reagent pump assembly The reagent pump assembly is mounted underneath. Built-in diaphragm pumps discharge reagents in each analytical cycle. Conductivity detectors The two conductivity detectors within the UFC ensure the proper aliquots of sample are in the shear face before dispensing the sample and reagent to the chambers. The RBC/Plt reaction chamber The RBC/Plt reaction chamber serves as a container where the RBC/Plt reagents and samples are mixed for the desired cytochemical reaction. The ADVIA® 2120i Hematology System hydraulics diagram is accessed in the Operator's Guide and is used to identify: Sample pathways Reagent pathways Valves used in sample pathways Valves used in reagent pathways Download and print a copy of the hydraulic flow diagram. Accessing and Using the Hydraulic Diagram Learn how to access and use the hydraulics diagram. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90005847/ADVIA2120_HydraulicDiagram_800x600_9/ADVIA2120_HydraulicDiagram_800x600_9.swfHTML5 File:/content/generator/Course_90005847/ADVIA2120_HydraulicDiagram_800x600_9/index.htmlPDF File: The ADVIA® 2120i Hematology System has three sample selectivity pathways: CBC CBC/diff CBC/diff/retic Selectivity Pathways Learn about sample selectivity pathways. Tab TitleTextCBC sample When just a CBC is run, valves V1, V47 and V72 are opened when aspiration is initiated. The vacuum pulls the sample through the shear valve to the second conductivity sensor.   CBC/diff sample When only a CBC/diff is run, valves V1, V47 and V73 are opened when aspiration is initiated. The vacuum pulls the sample through the shear valve to the second conductivity detector.  CBC/diff/retic sample When a CBC/diff/retic is run, valves V1, V47 and V74 are opened when aspiration is initiated. The vacuum pulls the sample through the shear valve to the second conductivity detector.  When complete, select the X in the upper-right corner to close the window and continue.   All sample aliquots and reagents are delivered to the appropriate reaction chamber through specific pathways. When the sample is aspirated it goes through the following: The sample first passes through the selector valve which has a clot filter It makes contact with the first conductivity detector and enters the shear valve It is aliquotted for the desired selectivities The sample then contacts a second conductivity detector. At this point the shear valve turns to the dispense position and the reagent pumps push the sample and reagent to the respective chambers.  The sample and reagents mix and the reaction occurs Note: Perox 1 reagent is used in the delivery of the sample to the Perox reaction chamber. Perox reagents 2 and 3 are delivered directly to the Perox reaction chamber.   Sample and Reagent Delivery Details Learn about the sample and reagent delivery to the Reaction Chamber. Slide NumberText BlocksCalloutsAudio ScriptImage File1 RBC sample and reagent delivery to the reaction chamber When the sample contacts conductivity detector 2, the shear valve moves to the dispense position and the RBC platelet reagent and sample are delivered to the RBC platelet reaction chamber. Select Next to continue. Note: If audio does not automatically start, select the play arrow in the top left to begin.When the sample contacts conductivity detector 2, the shear valve turns to dispense. The RBC platelet reagent comes into the shear valve and the sample and RBC platelet reagent are delivered and mixed in the RBC platelet reaction chamber.2Baso When the sample contacts conductivity detector 2, the shear valve moves to the dispense position and the Baso reagent and sample are delivered to the Baso reaction chamber.   Select Next to continue.  When the sample contacts conductivity detector 2, the shear valve turns to dispense. The Baso reagent comes into the shear valve and the sample and Baso reagent are delivered and mixed in the Baso reaction chamber. 3Retic When the sample contacts conductivity detector 2, the shear valve moves to the dispense position and the retic reagent and sample are delivered to the Retic reaction chamber.   Select Next to continue.  When the sample contacts conductivity detector 2, the shear valve turns to dispense. The retic reagent comes into the shear valve and the sample and Retic reagent are delivered and mixed in the Retic reaction chamber. 4Perox When the sample contacts conductivity detector 2, the shear valve moves to the dispense position and the Perox 1 reagent and sample are delivered to the Perox reaction chamber. Perox 2 and Perox 3 reagents are delivered directly into the Perox reaction chamber. You will see on the image displayed, the delivery path from sample shear valve to waste. Select Next to continue.  When the sample contacts conductivity detector 2, the shear valve turns to dispense. Perox 1 reagent comes into the shear valve and the sample and Perox 1 reagent are delivered to the Perox reaction chamber. Perox 2 and Perox 3 reagents are delivered directly to the Perox reaction chamber. 5 Hemoglobin When the sample contacts conductivity detector 2, the shear valve moves to the dispense position and the sample and Hgb reagent are delivered to the Hgb chamber. When complete, select the X in the upper-right corner to close the window and continue. When the sample contacts conductivity detector 2, the shear valve turns to dispense. The Hemoglobin reagent comes into the shear valve and the sample and hemoglobin reagent are delivered and mixed in the hemoglobin chamber. Not only does the reaction occur in the hemoglobin cuvette, but the reading is taken in the same cuvette. syngo.share is a unified, patient-centric clinical image sharing system. - It empowers healthcare institutions to manage and share clinical imaging data efficiently. - It can be used to manage and share DICOM, non-DICOM and multimedia data formats – like pdf, video or audio files - everything is kept in the native format. For instance, the data from sleeping labs remain active and can be used at any time. - syngo.share is a fully-fledged IHE-XDS and XDS-I compliant repository. And can thus be used to exchange data according to the IHE standard. - syngo.share can be deployed either as a departmental, an enterprise-wide or regional solution, due to its modular and scalable architecture. Shuttling is the process of moving the reaction to the flowcell for analysis. The shuttling process is the same for all reactions with only the necessary valves changing. Note: The hemoglobin is not shuttled. The reaction and the counting take place in the same cuvette. Shuttling and counting process Learn about the shuttling and counting process.   Select the play arrow to begin the video.  In this animation, you will watch the shuttling of the RBC/Platelet reaction. When complete, select the X in the upper-right corner to close the window and continue. The ADVIA® 2120i Hematology System constantly monitors the vacuum and pressure for proper delivery of the samples. Analyzer Status screen displays: Vacuum Pressure Adjust the pressures and vacuum Learn how to adjust the pressures and vacuum Checklist TitleChecklist TypeChecklist ContentAnalyzer StatusHTML Open the analyzer status screen tab in the Utilities menu. The pressure and vacuum readings are in the lower left corner. Select each checkbox to learn more about adjusting pressures and vacuum.  Access regulator knobsHTML   Select the play arrow to begin the video. Access the regulator knobs. Adjust pressures, vacuumHTML   Select the play arrow to begin the video. Adjust the regulator knobs to the desired reading. The response is not immediate. It may take a few moments for the reading to adjust. Continue to adjustHTML Continue to adjust the regulator knobs until the desired reading is attained.   Replace side panelHTML After the desired reading is attained, replace the side panel of the analyzer.   Ready to RunHTML To replace the side panel on the analyzer, align the pegs on the top of the panel with the holes on the system. Snap it in place. When complete, select the X in the upper-right corner to close the window and continue.   Welcome to the ADVIA® 2120i Hematology System How the System Works online training. In this course you will learn: How samples and fluids are moved through the ADVIA® 2120i system  About the tools available that can assist in locating the specific pathways Functions of the pathway components   ADVIA is the registered trademark of Siemens Healthcare Diagnostics Inc.  All other trademarks are the property of their respective owners. Select Next to continue.