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ADVIA® 2400 Chemistry System Overview Online Training

Overview of the main components and technology onboard the system.

Welcome to the ADVIA® 2400 Chemistry System Overview Online Training course. This training will provide you with an over of some of the key features and system components of the ADVIA® 2400 Chemistry system.   Upon successful completion of this course, you will be able to: Recognize system features of the ADVIA® 2400 analyzer List the three major system modules Identify the sampling options Identify how the ADVIA® 2400 identifies, stores and manages reagents Describe how the system assesses and handles the reagent inventory List the steps that take place during assay measurement Identify how the ISE module works Recognize the features of the Sample tray, Dilution tray and the Rack handler Congratulations.  You have completed the ADVIA® 2400 Chemistry System Overview Online Training course. ADVIA® 2400 System Overview Module and Components Sample Tray Dilution Tray Rack Handler Reagent Inventory Assay Measurement Reaction Tray Assay Methods ISE Module Overview ISE Methodology ISE Measurement Analytical Module ISE Module Rack Handler Sample Tray (STT/CTT) Dilution Tray (DTT) Reaction Tray (RRV) Reagent Tray 1 (RTT1) Reagent Tray 2 (RTT2) Dilution Probe (DPP) Sample Probe (SPP) Reagent Probe (RPP1) Reagent Probe (RPP2) The ADVIA® 2400 Chemistry System aspirates samples every two seconds from either the: Onboard Sample Tray Optional Universal Rack Handler Laboratory Automation System Barcoded samples in 5, 7, and 10mL tubes or sample cups can be aspirated using both the sample tray and the rack handler. The STT or sample tray has 84 positions which are not refrigerated.   In the STT, primary and secondary tubes can be intermixed.  All common tube sizes (5, 7, and 10mL) can be used.   The CTT has 61 refrigerated positions with an evaporation cover and is used for calibrators, controls, wash solutions, and special diluents. Load primary tubes on the Sample Tray Load primary tubes on the Sample Tray.   Samples delivered to Dilution Tray Samples delivered to Dilution Tray.   Loading Calibrators and Controls on the CTT Loading Calibrators and Controls on the CTT   Dilution Probe (DPP) Sample Probe (SPP) Reagent Probe (RPP1) Reagent Probe (RPP2) The sample delivered by the DPP resides in the Dilution Tray as a 1:5 dilution until all assays are complete.  This allows for further dilution, or re-runs if required. The SPP or Sample Probe aspirates the diluted sample from the DTT and dispenses it into the RRV (Reaction Tray). The RPP1 and RPP2 or Reagent Probes dispense reagents into cuvettes on the RRV (Reaction Tray). Dilution Tray or DTT The reusable cuvettes (120 total) are used to hold the diluted sample. Maximum fill volume is 300 µL DPP Aspirates Samples DPP Aspirates Samples   SPP Delivers Sample to the RRV SPP Delivers Sample to the RRV   Rack Handler: Exterior Samples aspirated from Rack Handler by Analytical Module   Universal Rack Handler Universal Rack Handler   The ADVIA® 2400 Chemistry System uses liquid reagents.  Most of the reagents on the system are ready to use and require no preparation. The reagents are packaged in barcoded wedges and stored in the two 50-position refrigerated reagent trays.  Each reagent tray has a reagent probe with liquid level sensing to aspirate the reagents and dispense them into the reaction tray. ADVIA® 2400 reagents are packaged in barcoded wedges containing the following information: Test Name Number of tests Lot Number Expiration Date 1st or 2nd reagent Multiple size wedges based on method requirements (70 mL or 20 mL) 70 mL 20 mL Deionized water ISE buffer Reaction bath oil Dilution bottle Cuvette conditioner Cuvette wash Load Reagent Wedges Load Reagent Wedges   Scan Reagents Scan Reagents   Reagent Probe Aspirates Reagents Reagent Probe Aspirates Reagents   Up to 700 tests performed from a 70 mL wedge More than one wedge of the same method can be loaded at one time The system switches automatically to the new reagent wedge when the first wedges is empty The reagent probes use liquid level sensing to determine the remaining volume in the reagent wedges This volume is then used to calculate the number of tests left in the wedges The ADVIA® 2400 Chemistry System has the ability to perform a large number of methods and applications.  Samples are analyzed using the advanced optical system on the ADVIA 2400. The system performs both immuno-turbidimetric methods, as well as, the traditional photometric chemistry methods. Serum indices and sample blank correction are also available onboard the system. The Reaction Tray has 340 reusable, plastic cuvettes It uses 20 segments of 17 cuvettes each Sample and Reagent Probes Sample and Reagent Probes   15 µL to 150 µL of reagent 1 is added to the reaction tray cuvette.  The cuvette is maintained in a 37°C inert hydrofluorocarbon oil bath Depending on the test, 2 µL to 25 µL of the diluted sample is dispensed into the cuvette The cuvette contents are mixed If required by the method, the second reagent is added and mixed Cuvette contents are allowed to incubate in the reaction tray for 5 - 10 minutes The cuvettes are read by the spectrophotometer Upon completion of the reaction, the cuvette is washed and dried.  A cuvette integrity checked is performed before they are used again Endpoint assays Reaction rate assays 2-point rate assays Homogeneous immunoassay Serum indices and sample blank correction Prozone (antigen excess) check The ADVIA® 2400 Chemistry System utilizes Ion Selective Electrode (ISE) technology to measure the electrolyte (Sodium, Potassium, and Chloride) concentration in serum, plasma, or urine. All three ISE measurements are taken simultaneously at a rate of 600 tests per hour. A separate aspiration of 22 µL of the original sample is taken for the electrolytes before the photometric sample is aspirated. The sample is dispensed into the ISE dilution bowl where a dilution with ISE Buffer is made. The diluted sample is used by the ISE's to determine the concentration of Sodium, Potassium, and Chloride in the sample. ISE Electrodes Reference Electrode ISE Cell Pot ISE Buffer Solution Dilution Probe (DPP) Buffer Line Heater Motor The dilution probe aspirates 22 µL of undiluted sample for the measurement of Na+, K+, and Cl--. The sample is dispensed into the ISE dilution bowl where the ISE buffer is added and mixed to create a 1:33 dilution. The diluted sample passes through the Ion Selective Electrodes (ISE) to obtain voltage readings. The difference in the voltages of the sample and reference are used to calculate the concentration of Na+, K+, and Cl--. The ISE channels are washed with ISE buffer to prepare them for the next analysis.

  • SPP
  • RRV
  • DPP
  • CTT
  • DTT
  • STT
  • ISE
  • RTT
  • RPP