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ADVIA Centaur® CP Immunoassay System Hardware Overview Online Training

Hardware overview includes locating major system components and determining their function to provide the operator the foundation to successfully operate the ADVIA Centaur® CP Immunoassay System. This clinical laboratory training qualifies for continuing education units (CEU).

Welcome to the ADVIA Centaur® CP Immunoassay System Hardware Overview Online Training course.   The ADVIA Centaur® CP System features: 180 tests / hour Wide range of disease testing Specialty & routine tests Continuous operation     Select Next to continue. Describe the different types of hazards associated with routine operation Identify the components required for sample analysis Identify the reagent handling components Identify the sample handling components Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations. You have completed the Hardware Overview online training course. Listed below are the key points that have been presented. Take time to review the material before proceeding to the final quiz. Identify the sample handling components Sample racks are loaded into the sample compartment. The compartment can accommodate up to 7 racks. Each sample rack can hold up to 12 samples. The sample probe picks up a disposable tip from a tip drawer and aspirates sample from a rack. The sample probe dispenses the sample into a cuvette in the incubation ring. Cuvettes are loaded into the cuvette loading bin on the right side of the system. Identify the reagent handling components Primary and ancillary reagents are loaded into the reagent compartment. The reagent compartment can accommodate up to 15 primary packs and 10 ancillary reagent packs. The reagent probe aspirates from a reagent pack and dispenses into a cuvette in the incubation ring. Acid and base reagent are key components in the chemiluminescent reaction. The acid and base compartment is located on the left side of the instrument. The fluidics area is composed of the DI water container, Wash 1 container and liquid waste container.   Identify the components required for sample analysis Sample and reagent are added to cuvettes in the incubation ring. After incubation has occurred, cuvettes are moved to the wash station. In this area, unbound reagent is removed from the cuvettes by aspirate probes. The cuvettes are then washed with Wash 1 and acid is delivered when washing is complete. After acid is added to the cuvette, an elevator delivers the cuvette to the luminometer where the base probe dispenses base and the chemiluminescent reaction occurs. The waste probe then removes any liquid waste from the cuvette and the ejector rod pushes the cuvette into the solid waste bin. Describe the different types of hazards associated with routine operation Biohazards  include the sample compartment, solid waste drawer, liquid waste container and primary reagents. Wear PPE when handling these biohazards. Chemical hazards include acid and base reagent, cleaning solution, primary and ancillary reagents.  Wear PPE when handling these components. Laser exposure can occur from the sample compartment and reagent compartment. Avoid looking directly into the beam. Hazards associated with moving parts, such as the sample and reagent probes.  Turn the instrument off before opening the top over.     Select Next to continue.   In this section we will learn about: Sample Compartment Sample Rack LED Status lights Sample Probe Tip Drawers Cuvette Loading Bin Sample Handling Learn about Sample Handling. Slide NumberText BlocksCalloutsAudio ScriptImage File1 Sample Compartment: Located on the front of the analyzer Holds up to 7 racks Accommodates up to 84 samples Select Next to continue.Select each number to review the corresponding text.Note: If audio does not automatically start, select the play arrow in the top left to begin. CalloutsSample CompartmentSample RackTube Type SelectorLED Status lightsThe sample compartment is located on the front of the instrument. To open the sample compartment, pull gently on the top of the compartment door. The sample compartment is capable of holding up to 7 racks. Each individual rack is capable of holding 12 sample tubes. This means that the system can accommodate a total of 84 samples. Let’s turn our attention, briefly, to the racks themselves. Racks are used to load samples, quality control material and calibrator material. Racks can accommodate one tube type at a time. The tube type selector allows for tube type selection. Adjust it by turning the knob to the desired tube type to be used. In this case, you can see that the Tube Type Selector is set to tube type “C”. Tube types “A” and “B” are also available. The Sample Compartment holds seven lanes that guide the sliding of the racks into the compartment, and light-emitting diodes, or “LEDs” on the system cover indicate whether a lane is occupied or not. The LEDs are an important mechanism to communicate the status of lanes and operation of the instrument.2 System Components: Sample Probe Tip Drawers Cuvette Loading Bin  Select Next to continue. Select each number to review the corresponding text.CalloutsSample ProbeTip DrawersCuvette Loading BinWith samples loaded in the Sample Compartment, the Sample Probe is the component that starts the test sequence. You can see the Sample Probe by looking through the cover window. Let’s begin by looking at the use and location of tips and cuvettes. 3 Sample Tips: 2 Tip Drawers  Each Drawer holds 240 tips for a total of 480 tips  Disposable Sample Tips eliminate carryover Select Next to continue. There are two Tip Drawers. Each drawer holds 240 tips, this means the system holds a total of 480 tips. You will see how to load tips in a subsequent course, but, for now, the tips are an important component because the system uses these disposable sample tips to eliminate any possibility of sample carryover. The tips are picked up by the Sample Probe which then aspirates a sample in the sample compartment. The probe transports the sample and dispenses it into a cuvette in the incubation ring where the reaction takes place. In the end, the sample tip is ejected into the Solid Waste Drawer. 4 Cuvette Loading Bin: Right side of the system Holds 400 cuvettes Loaded anytime during operation When complete, select the X in the upper-right corner to close the window and continue.The cuvette loading bin is located on the right-hand side of the system and can hold 400 cuvettes. Cuvettes are packaged in bags of 200. Cuvettes can be loaded at any time during system operation. The system moves cuvettes to the cuvette loader which correctly positions the cuvettes before they enter the incubation ring. In this section we will learn about: Reagent Compartment Primary and Ancillary Reagents LED Status Lights Acid and Base Reagent Compartment Fluidics Area Reagent Handling Learn about Reagent Handling. Slide NumberText BlocksCalloutsAudio ScriptImage File1 Reagent Compartment: Located on the front of the system Holds 15 primary reagents Holds 10 ancillary reagents Primary Reagents: Paramagnetic particles Acridinium Ester (AE) Ancillary Reagents: Diluents Releasing Agents Select Next to continue. Note: If audio does not automatically start, select the play arrow in the top left to begin.The Reagent Compartment is located on the front of the system. Pulling the top of the door gently will open the compartment. Primary and ancillary reagents perform different functions. Primary reagents contain solid and lite reagents. Solid reagent contains paramagnetic particles and lite reagent contains the label Acridinium Ester or AE. Ancillary reagent packs contain diluents and releasing agents. It is important to know where this compartment is located and the existence and use of both primary and ancillary reagents. In the subsequent course, you will learn how to load reagents.2 Light-emitting diodes or LEDs: Located above tip drawers, sample compartment and reagent compartment Indicate status of supplies Select Next to continue. Select the number to review the corresponding text.CalloutsLED Status Bar Now, let’s look at the light-emitting diodes or LEDs. These LEDs on the front cover above the tip drawers, sample compartment and reagent compartment illuminate to indicate the status of the supplies within. When you begin to use the system, we will focus on what the LEDs communicate. Refer to the ADVIA Centaur® CP system, Operator’s Guide LED Color Coding for further information.3 Reagent Probe: Visible when the cover is lifted Aspirates reagents Dispenses reagents Select Next to continue. If the cover to the instrument is lifted, you will see the reagent probe. The incubation ring moves the cuvette from the Sample Probe area to the Reagent Probe area. The Reagent Probe aspirates the primary and ancillary reagents from the packs in the reagent compartment and dispenses the reagents into the cuvette in the incubation ring. After each dispense of reagent, the probe is rinsed with water.4 Acid & Base Reagent Compartment: Located on the left side of the instrument. Contains Acid and Base Reagents. Select Next to continue. Select each number to review the corresponding text.CalloutsR1 is the Acid bottle.R2 is the Base bottle.An important component of the Reagent Handling capability of the ADVIA Centaur® CP is the Acid and Base Reagent Compartment. This compartment is located on the left-hand side of the instrument. This area contains the acid and base reagents that are used to trigger reactions during analysis. 5 Fluidics Area: Liquid Waste Wash 1 Solution De-ionized Water (DI Water) Select the image areas to learn more about where the Acid and the Base bottles are located.When complete, select the X in the upper-right corner to close the window and continue.CalloutsLiquid WasteWash 1 SolutionDI WaterThe containers used to supply the de-ionized water (DI Water) and Wash 1 Solution as well as liquid waste removal are commonly referred to as the Fluidics Area. The three containers can be placed on the same level as or below the system. Each container has a different colored top. The red top is the liquid waste. You will notice a biohazard sign. The bottle with the white top is Wash 1, as indicated on the label. The third bottle labeled H20 is DI Water, and has a blue top. In this section we will learn about: Incubation Ring Wash Station Luminometer       Sample Analysis Learn about Sample Analysis. Slide NumberText BlocksCalloutsAudio ScriptImage File1 The Incubation Ring: Heated to 37°C (98.6° F) Holds 80 cuvettes Advances cuvettes at 20 second intervals Moves cuvette from Sample Probe to Reagent Probe   Select Next to continue.Note: If audio does not automatically start, select the play arrow in the top left to begin.The Incubation Ring is a circular, insulated track heated to a temperature of 37 degrees Celsius or 98.6 degrees Fahrenheit. This temperature is required in preparation for the reaction to follow. The system correctly positions cuvettes before they enter the incubation ring; which holds up to 80 cuvettes in total. The incubation ring advances the cuvettes at 20-second intervals. As you have learned, it moves a cuvette from the Sample Probe to the Reagent Probe and onwards. 2 The Wash Station: Aspirate Probes draw fluid out of the cuvette Wash Station delivers Wash 1 into cuvette Multiple washes occur if necessary Cuvette is moved to acid probe Acid reagent is dispensed into the cuvette   Select Next to continue. Select each number to review the corresponding text.CalloutsAspirate ProbeAspirate ProbeAcid ProbeAs we continue to focus on analysis and the system interior, let’s take a look towards the back of the instrument at the location and function of the Wash Station. A magnet, positioned along the incubation ring, pulls the paramagnetic particles, or iron oxide crystals, to the side of the cuvette. The aspirate probes then draw fluid out of the cuvette and the wash station delivers Wash 1 into the cuvette to wash the magnetic particles. If necessary, multiple washes occur. The incubation ring then moves the cuvette to the acid probe, which dispenses acid reagent into the cuvette. 3 The Luminometer: Base Probe PMT measures Relative Light Units (RLUs) Work station processes data into results Waste Probe Ejector Rod When complete, select the X in the upper-right corner to close the window and continue.Select each number to view the corresponding text. CalloutsLuminometerPhotomultiplier Tube (PMT)Waste ProbeEjector RodAfter passing through the wash station, the cuvette continues its journey inside the system to the next step in sample analysis. The incubation ring moves the cuvette to an elevator, which lifts the cuvette from the incubation ring into the luminometer. The cuvette enters the luminometer, where the chemiluminescent reaction occurs and is measured. The luminometer has three parts. The Base Probe dispenses base reagent into the cuvette. After the chemiluminescent reaction has occurred, the photomultiplier tube, or PMT, measures the light emitted as relative light units or RLUs. The workstation processes the data from the PMT and converts the RLUs into results. Finally, the waste probe aspirates the waste fluid from the cuvette and the ejector rod pushes the cuvette into the solid waste drawer. The initial stages of the sample testing sequence can be organized into three steps: The sample probe picks up a tip It aspirates a sample in the sample compartment It dispenses the sample into a cuvette in the incubation ring Note: Do not open the cover while the instrument is running. Test Sequence Checklist Learn about the steps in the Test Sequence. Checklist TitleChecklist TypeChecklist ContentPick up TipHTML The Sample Probe picks up a tip. Select each checkbox to learn more about the steps in the Test Sequence.Aspirates a sampleHTML The probe aspirates a sample in the sample compartment. Dispenses the sampleHTML The Sample Probe dispenses the sample into a cuvette in the Incubation Ring. When complete, select the X in the upper-right corner to close the window and continue. Types of Hazards: Biohazard Exposure Chemical Exposure Laser Exposure Hazards associated with moving parts Select Next to continue. Areas of Exposure: Sample Compartment Solid Waste Drawer Liquid Waste Container Primary Reagents Biohazard slideshow Learn about biohazard sources and prevention. Slide NumberText BlocksCalloutsAudio ScriptImage File1 The Sample Compartment:        Samples, controls, and calibrators can spill or splash This can cause contamination      Select Next to continue. Select each number to review the corresponding text.Note: If audio does not automatically start, select the play arrow in the top left to begin.CalloutsSample CompartmentSolid Waste DrawerOne obvious source of biohazard exposure is the sample racks and sample compartment on the analyzer. Samples, controls, and calibrators can spill or splash and cause contamination of these areas. 2 Solid Waste Drawer:  Sample tips and cuvettes are dispensed into this drawer  Drawer emptied at the beginning of each day and as needed Select Next to continue. Note:  The Solid Waste Drawer is emptied at the beginning of each day and as needed.Select each number to review the corresponding text.CalloutsSolid Waste Drawer Sample CompartmentAnother area of biohazard concern is the solid waste drawer. Sample tips and cuvettes are dispensed into this drawer after use. You will empty this drawer at the beginning of each day and as needed throughout. 3 Liquid Waste Container:   Collects liquid waste from the cuvettes and probe wash stations Container emptied regularly Select Next to continue. The liquid waste container collects liquid waste from the cuvettes and probe wash stations and is our final source of biohazard exposure. This container is also emptied regularly. 4Primary Reagents: May contain human source material Should be handled as potentially infectious Refer to assay IFU - Reagents section for more informationSelect Next to continue.Reagent packs could be a potential biohazard. Some reagents contain human source material. While each human serum or plasma donor unit used in the manufacture of this product was tested by FDA-approved methods and found nonreactive for hepatitis B surface antigen, antibody to hepatitis C, and antibody to HIV-1/2, all products manufactured using human source material should be handled as potentially infectious. Since no test method can offer complete assurance that hepatitis B or C viruses, HIV, or other infectious agents are absent, these products should be handled according to established good laboratory practices. Refer to the Reagents section of the assay IFU for more information.5 Biohazard Protection:  Always Wear PPE (Gloves, Lab Coat and Safety Glasses) Follow laboratory biohazard protection procedures   When complete, select the X in the upper-right corner to close the window and continue.Note:  Always follow your Laboratory Biohazard Protection Procedures.To prevent or minimize exposure to biohazards follow some simple guidelines. Always wear Personal protective equipment, or PPE, such as gloves, lab coat and safety glasses while working on the analyzer. Always adhere to your laboratory’s biohazard protection procedures. For more information on Siemens’s biohazard protection recommendations see the Safety Information Appendix of the ADVIA Centaur® CP Immunoassay System Operator’s Guide. Sources of Chemical Hazards:  Acid Reagent (R1)  Base Reagent (R2)  Cleaning Solution  Primary Reagents  Ancillary Reagents Chemical Hazard Slideshow Learn about chemical hazard sources and prevention. Slide NumberText BlocksCalloutsAudio ScriptImage File1Acid Reagent (R1): Contains hydrogen peroxide and a very dilute Nitric acid solution May cause irritation Extended skin contact and eye contact should be avoided Base Reagent (R2): Located next to the acid reagent Composed of dilute or 1% sodium hydroxide and a surfactant Avoid skin and eye contact Select Next to continue. Select each number to review the corresponding text.Note: If audio does not automatically start, select the play arrow in the top left to begin.CalloutsAcid Reagent (R1)Base Reagent (R2)The acid reagent, located on the left side of the instrument, contains hydrogen peroxide and a very dilute Nitric acid solution. The acid concentration is only 0.5%; but, the solution may cause irritation and extended skin contact and eye contact should be avoided. The base reagent, located next to the acid reagent, is composed of dilute or 1% sodium hydroxide and a surfactant. Base reagent is an irritant and skin and eye contact should also be avoided. 2Cleaning Solution: Contains 5% percent sodium hypochlorite Can cause irritation to skin and eyes Select Next to continue. Select the number to review the corresponding text.CalloutsCleaning SolutionCleaning solution is used during some of the ADVIA Centaur® CP maintenance procedures. The concentrated solution contains 5% percent sodium hypochlorite. It is diluted before use on the system. This reagent can also cause irritation to skin and eyes. 3Primary Reagents: May contain Sodium azide Ancillary Reagents: May contain sodium hydroxide   For more information about chemical hazards in reagents, refer to the assay IFU - Reagents section. Select Next to continue.  Some primary reagents may contain sodium azide in the lite reagent or solid phase. Sodium azide is harmful if swallowed and can cause skin irritation. Some ancillary reagents may contain sodium hydroxide which can be irritating to the eyes or skin. 4Chemical Hazard Protection PPE: Laboratory Coat Protective Gloves Safety Glasses or Face Shield When complete, select the X in the upper-right corner to close the window and continue. To prevent or minimize exposure to these chemical irritants always wear PPE. A lab coat, protective gloves, and safety glasses or a face shield are appropriate when working with hazardous chemicals. Sources of Laser Exposure: Sample Compartment Barcode Scanner Reagent Barcode Scanner Laser Exposure Slide Show Learn about laser exposure sources and prevention. Slide NumberText BlocksCalloutsAudio ScriptImage File1 Sample Barcode Scanner:    Located within the sample compartment Warning symbol inside compartment door Type 2 lasers can cause retinal damage if the beam directly hits the eye Beam is located to the left of the compartment     Select Next to continue   Select each number to review the corresponding text.Note: If audio does not automatically start, select the play arrow in the top left to begin.CalloutsSample Barcode ScannerLaser warning symbolThe sample barcode reader is located within the sample compartment. A yellow laser warning symbol and label is affixed to the inside of the compartment door as a reminder. Type 2 lasers can cause retinal damage if the beam directly hits the eye. Do not stare into the laser beam. This beam is located to the left of the compartment. 2Reagent Barcode Scanner: The reagent compartment utilizes laser readers Symbol and warning is affixed inside the door One beam located at the back of the compartment Other laser located to the left of the compartment  Select Next to continueThe reagent compartment also utilizes laser readers to scan the barcodes of the ancillary and primary reagent packs. A similar symbol and warning is affixed inside the door. One beam is located at the back of the compartment and is shielded from view. The other laser is located to the left of the compartment. Again, avoid staring into this beam. 3Laser Safety Procedures:  Never look directly at the laser beam or its reflection from a shiny surface Only Siemens-trained Service personnel should perform procedures related to lasers When complete, select the X in the upper-right corner to close the window and continue.Follow some simple guidelines to protect yourself from harmful laser exposure. Heed the laser warning label instructions to never look directly into a laser beam or its reflection from a shiny surface. Never try to service a laser yourself. Only Siemens-trained service personnel should perform laser-related procedures. Moving Component Exposure: Front Doors Side Door Top Cover Moving Component Slideshow Learn about moving component sources and prevention. Slide NumberText BlocksCalloutsAudio ScriptImage File1 Front Doors:   Allow access to the tips, solid waste, sample, and reagent areas Can be opened anytime to allow removal and addition of supplies, samples and reagents Select Next to continue.  Select each number to review the corresponding text.Note: If audio does not automatically start, select the play arrow in the top left to begin.CalloutsTip DrawersSample CompartmentReagent CompartmentSolid Waste DrawerThe front doors allow access to the tips, solid waste, sample, and reagent areas. The doors can be opened anytime to allow removal and addition of supplies, samples and reagents. Subsequent courses show how to check status light indicators above the doors prior to adding or removing supplies or reagents. 2 Side Door: Can be opened to allow troubleshooting of the cuvette bin Select Next to continue. Select the number to review the corresponding text.CalloutsCuvette Loading Bin The right side door can be opened at anytime to allow troubleshooting of the cuvette bin. 3Top Cover: Allows access to internal components of the analyzer. Has an interlock switch that stops moving mechanisms if lifted while the system is processing samples Instances when cover must be opened to perform maintenance or repair procedures Select Next to continue.Select the number to review the corresponding text.CalloutsTop CoverFinally, the top cover allows access to all of the internal components of the analyzer. This cover also has an interlock switch that stops moving mechanisms if it is inadvertently lifted while the system is processing samples. There are instances when this cover must be opened to perform maintenance or repair procedures.4Injury Prevention: Do not open top cover with power ON to the analyzer Perform maintenance procedures with power OFF When complete, select the X in the upper-right corner to close the window and continue.To prevent exposure to moving components and possible injury follow some simple guidelines. First, never open the top cover with the power On to the analyzer. Make sure that the power switch on the left side of the instrument is in the OFF position. Only perform maintenance procedures in the OFF or Ready state as per instructions in the ADVIA Centaur® CP Operator's Guide. Never perform maintenance while the instrument is processing samples.

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