ADVIA Centaur® CP Immunoassay System Sample Processing Online Training

O corpo tem a capacidade de eliminar produtos químicos a partir de uma ou outra extremidade da equação de tampão de bicarbonato para manter o pH constante. Na equação, o ácido carbônico é rapidamente convertido em dióxido de carbono e água, em resposta a hipo ou hiperventilação. Welcome to the ADVIA Centaur® CP Sample Processing Online Training course. The procedures covered in this course include: How to load and schedule routine samples How to load and schedule STAT samples How to start the sample run How to monitor the progress of a sample run How to pause or stop the run as needed Select Next to continue. Describe the sample loading process List the steps to start sample analysis Use the Incubation Ring window Describe the events of the test analysis sequence Recognize the actions and consequences of pausing or stopping a sample run Schedule routine and STAT samples Upon successful completion of this course, you will be able to: Select Next to continue. A principal função do sistema respiratório é a troca de oxigênio e dióxido de carbono dos pulmões. Existem quatro funções de respiração, ou a troca gasosa pulmonar: Ventilação, Perfusão, distribuição, difusão. Congratulations.   You have completed the Sample Processing online training course. Listed below are the key points that have been presented. Take time to review the material as needed before proceeding to the final quiz.. Describe the sample loading process Load sample tubes into racks Load racks into the sample compartment Manually enter misread IDs Remove completed sample racks Schedule routine and STAT samples Scheduling samples includes: Receiving orders from a host computer Creating manual orders Ordering Replicates Creating Batch orders Assigning sample IDs without barcodes STAT samples can be scheduled by: Using the LIS Using the Sample Compartment screen Using the STAT lane Identify the steps to start sample analysis Select Start Select OK Order the events of the test analysis sequence      Step 1: A cuvette is moved to the Incubation Ring      Step 2: The Sample Probe aspirates, then dispenses the sample      Step 3: The Reagent Probe aspirates, then dispenses reagents      Step 4: The cuvette incubates per the test protocol      Step 5: The cuvette is washed at the Wash Station      Step 6: Acid is added to the cuvette      Step 7: The cuvette is lifted to the Luminometer      Step 8: Base Reagent is dispensed and chemiluminescent flash occurs      Step 9: The fluid is aspirated from the cuvette and waste is discarded Identify uses of the Incubation Ring window  Boxes represent individual cuvette positions  Boxes with tests in progress contain test information such as the test name, sample ID and time to final result Recognize the actions and consequences of pausing or stopping a sample run To pause a sample run (tests in progress will continue):      1.   Access the Sample Compartment screen      2.  Select Pause      3.  To resume sampling, select Resume To stop a sample run (some tests in progress could be lost):       1.  Select Stop from the Task Bar      2.  Select OK from the emergency stop window Select Next to continue.     Os eletrólitos são moléculas carregadas positivamente e moléculas carregadas negativamente chamados íons que se encontram no interior das células do corpo e fluidos extracelulares, incluindo o plasma sanguíneo. Um teste para eletrólitos inclui a medição de sódio, potássio, cloreto e cálcio para diagnosticar e gerir renal, endócrino, ácido-base e de muitas outras condições. Ver os auxílios de trabalho para aprender sobre tópicos adicionais associados com o metabolismo de eletrólitos. The sample loading process includes: Loading sample tubes into racks Loading racks into the sample compartment Manually entering misread IDs Removing completed sample racks Select Next to continue.   Parabéns! You have completed the TRUGENE HIV-1 Genotyping Online Training course. Abaixo estão listados os principais pontos que foram apresentados. Reserve um tempo para revisar o material antes de prosseguir para o teste final. Several types of sample tubes can be used on the ADVIA Centaur CP system. Up to 12 tubes can be loaded into one rack. All samples in the rack must be the same size and type of sample tubes. The tube type selector on the sample rack is used to assign a tube type. Load Sample Tubes in Rack Checklist Learn how to load sample tubes into racks. Checklist TitleChecklist TypeChecklist ContentCheck the tube type selector for proper positionHTML Move the tube type selector on the rack to the correct position for the sample tubes you are loading in the rack.  It can be turned to position A, B or C.  Each letter is assigned to a specific tube type and size in the ADVIA Centaur® CP software. To view and set tube types, refer to Setting Tubes Types in the Operating the System section of the Operator's Guide. Select each checkbox to learn more about Loading Sample Tubes into Racks.Check the tube type and barcode placementHTML The label must be placed vertically on the tube, about  2 cm from the top of the tube.Place the tubes into the sample rackHTML Make sure that the tube barcode label is facing the same side as the rack labels. The tube barcode label should be completely visible above or between the slots of the rack. Make sure that the tube is pressed all the way down into the rack and that the cap is removed.   Position 1 is located farthest away from and position 12 closest to the handle of the rack.  It is not necessary to fill every position in the rack. When complete, select the X in the upper-right corner to close the window and continue. A Case can now be created once the eight assay files have been analyzed. Select the link below to learn how to combine assay files in the GeneObjects software. Once racks are prepared, they can be loaded into the system for processing. Load Racks Checklist Learn how to load racks into the sample compartment. Checklist TitleChecklist TypeChecklist ContentOpen the sample compartment doorHTML Open the Sample compartment door.  Select each checkbox to learn more about Loading Racks into the Sample Compartment. Locate the lane with a flashing green LEDHTML A flashing green LED indicates the lane is ready to receive the next sample rack. Slide the rack smoothly into placeHTML Align the slot located at the bottom of the Sample Rack with the Lane Guide Bar. Slide rack smoothly forward with one continuous motion. Push gently to lock into place. If the rack stops or reverses during insertion, the barcodes may not readClose sample compartment doorHTML If the rack barcode label is correctly recognized, the green flashing light will extinguish or go to the neutral state and a green flashing light will appear above the next ready lane. If the rack barcode is not recognized, the green flashing light will change to a yellow flashing light. When all racks are loaded, close the sample compartment door.  When complete, select the X in the upper-right corner to close the window and continue. Misread Sample Barcodes: Green flashing lane LED will extinguish Sample ID will be missing from a rack position Manual entry of the sample ID is required Misread IDs Learn how to manually enter misread IDs. Checklist TitleChecklist TypeChecklist ContentView the sample compartment windowHTML Check for misread sample barcodes by viewing the sample compartment screen as you insert the sample racks.  Select each checkbox to learn more about Manually Entering Misread IDs.Identify misread positionsHTML In this example a rack inserted into lane number 1.   A misread barcode will be identified by a blank white ID field in the rack. Blank grey positions are rack positions without tubes. Position number 1 of rack 1 has a blank white ID field indicating it was misread. Remove the sample rack and select RecallHTML Remove the sample rack to determine the correct ID for that position.  As the rack is removed, the screen will change. The Sample Compartment screen changes to show an empty lane. You will notice that the Recall button becomes active. Use the Recall button to recall the ID numbers of the removed rack.  Enter the missing ID numberHTML When the Recall window opens, select the blank position and enter the correct ID number. The missing ID can be either typed in or scanned in using the hand-held barcode reader.  Replace the sample rackHTML Return the rack into the same lane it was removed from. The recall window automatically closes and the manually entered ID appears in the rack. Continue to view racks for any other missing IDs. As they are detected, remove each rack individually, select recall, enter missing IDs and then replace the rack. The recall button retains only the last rack removed. When complete, select the X in the upper-right corner to close the window and continue. Misread IDs Practice manually entering misread IDs. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003367/sim_CP_Recall_800x600_9/sim_CP_Recall_800x600_9.swfHTML5 File:/content/generator/Course_90003367/sim_CP_Recall_800x600_9/index.htmlPDF File: Removing completed sample racks: Racks can be removed while the analyzer is running or processing samples Only remove completed sample racks Allows room for additional samples Remove Completed Sample Racks Learn how to remove completed sample racks Checklist TitleChecklist TypeChecklist ContentOpen the sample compartment doorHTML Open the sample compartment door.  If the analyzer is running or actively processing samples, the currently active racks are indicated by a steady green light above the sample rack lane. Select each checkbox to learn more about Removing Completed Sample Racks. Ensure that the status LED is neutralHTML Once a rack is complete, the green light extinguishes and reverts to the neutral or inactive state. Only remove racks when the light is neutral. It is important to never attempt to remove a rack when it is actively processing. Doing so could cause damage to the system and/or loss of sample results.Grasp the rack by the handle and removeHTML Since the rack locks into place when it is inserted, a slightly greater force at the beginning of the pull may be needed to unlock the rack for removal.Close the sample compartment doorHTML After all completed racks are removed, close sample compartment door. When complete, select the X in the upper-right corner to close the window and continue. The scheduling routine samples section will include: Receiving orders from a host computer Creating manual orders Ordering Replicates Creating Batch orders Assigning sample IDs without barcodes Select Next to continue. Orders can be downloaded from the host computer: Automatically when the analyzer sees the barcode and queries the Host View Automatically Downloaded Orders Learn how to view automatically downloaded orders. Checklist TitleChecklist TypeChecklist ContentSelect ResultsHTML From the workspace, select the Results button. Select each checkbox to learn more about Automatically Downloading Orders.Select the date columnHTML The Today’s tab will automatically open to show a list of records from the prior 24 hours. Select the Date column header to point the small gray arrow down and sort the most recent orders to the top of the list.View "Pending" ordersHTML Orders awaiting processing will have a “Pending” status in the RLU column. When complete, select the X in the upper-right corner to close the window and continue.   Reasons for manual order entry include:. A host computer is not connected to the analyzer The host computer is down An order needs to be manually modified Samples do not have barcode labels Manual Order Entry with Barcodes Learn how to manually enter orders for samples with barcodes. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003367/sim_CP_Schedule800x600_9/sim_CP_Schedule800x600_9.swfHTML5 File:/content/generator/Course_90003367/sim_CP_Schedule800x600_9/index.htmlPDF File: Manual Sample ID and Order Entry Learn how to manually enter sample IDs and orders. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003367/sim_CP_SchedNoBarcodes800x600_9/sim_CP_SchedNoBarcodes800x600_9.swfHTML5 File:/content/generator/Course_90003367/sim_CP_SchedNoBarcodes800x600_9/index.htmlPDF File: Pre-scheduling Orders Learn how to manually pre-schedule orders before the sample is loaded. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003367/sim_CP_SchedWorklist800x600_9/sim_CP_SchedWorklist800x600_9.swfHTML5 File:/content/generator/Course_90003367/sim_CP_SchedWorklist800x600_9/index.htmlPDF File: Ways to schedule a STAT sample include: Using host download Using the Sample Compartment screen Using the STAT lane Using Host Download Learn more about using the host download to schedule a STAT. Slide NumberText BlocksCalloutsAudio ScriptImage File1The Sample Compartment screen identifies a STAT by: An asterisk next to the sample type An orange box below the sample type Select Next to continue.Note: If audio does not automatically start, select the play arrow in the top left to begin.When the Host computer downloads a STAT order, the STAT sample can be easily recognized in the Sample Compartment screen. The STAT will have an asterisk next to the sample type and an orange box below it.2The Results STAT tab includes: Pending orders Results in process Completed Results Select Next to continue. You can view a list of STATs by accessing the Results STATs tab. This list includes Pending orders, results in process and completed results. 3The Results STAT tab can be sorted. Click on the Date column to move pending orders to the top of the list.   When complete, select the X in the upper-right corner to close the window and continue. Select the Date column to point the gray arrow down to sort any pending orders to the top of the list. Using the Sample Compartment Screen Learn how to use the Sample Compartment screen to schedule a STAT. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003367/sim_CP_STATSampComp800x600_9/sim_CP_STATSampComp800x600_9.swfHTML5 File:/content/generator/Course_90003367/sim_CP_STATSampComp800x600_9/index.htmlPDF File: Using the STAT Lane Learn how to use the STAT lane. Checklist TitleChecklist TypeChecklist ContentOpen Sample Compartment doorHTML Open the sample compartment door. Select each checkbox to learn more about using the STAT lane.Select LaneHTML The Sample Compartment screen opens automatically when the sample compartment door is open. The green LED will blink in the first available lane.  In this example, lane 2. In order to load the rack in the STAT lane, select the lane number.  By default the STAT lane is 7.Load RackHTML When the LED is blinking green above the STAT lane, you can load the sample rack.Review SamplesHTML Every sample in the rack will now be ordered as a STAT. When complete, select the X in the upper-right corner to close this window and continue. Starting a sample run: 1.Select Start 2.Select OK Starting the Run Practice starting a sample run. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003367/sim_CP_Start800x600_9/sim_CP_Start800x600_9.swfHTML5 File:/content/generator/Course_90003367/sim_CP_Start800x600_9/index.htmlPDF File: Now that the system is running samples, let's take a close look at the test analysis sequence.        Step 1: A cuvette is moved to the Incubation Ring      Step 2: The Sample Probe aspirates, then dispenses the sample      Step 3: The Reagent Probe aspirates, then dispenses reagents      Step 4: The cuvette incubates per the test protocol      Step 5: The cuvette is washed at the Wash Station      Step 6: Acid is added to the cuvette      Step 7: The cuvette is lifted to the Luminometer      Step 8: Base Reagent is dispensed and chemiluminescent flash occurs      Step 9: The fluid is aspirated from the cuvette and waste is discarded Test Analysis Sequence Learn more about the details of each step. Slide NumberText BlocksCalloutsAudio ScriptImage File1Step 1:  A cuvette is moved to the Incubation Ring. SelectSel ....Select Next to continue.Note: If audio does not automatically start, select the play arrow in the top left to begin.The incubation ring is typically full of cuvettes. If necessary, the system moves cuvettes from the loading bin to the cuvette loader which correctly orients the cuvettes and releases them into the incubation ring. The incubation ring is kept at 37 degrees celcius and advances the cuvettes in 20 second intervals.2Step 2:  The Sample Probe aspirates, then dispenses the sample into a cuvette in the incubation ring. Select Next to continue.Next, the sample probe picks up a sample tip from the tip tray to begin the test sequence. It moves to the sample compartment, aspirates a sample and then dispenses into a cuvette in the ring.3Step 3:  The Reagent Probe aspirates, then dispenses reagents into a cuvette in the incubation ring. Select Next to continue.     The incubation ring moves from the sample probe area to the reagent probe area. Here the reagent probe aspirates primary and/or ancillary reagents from the packs in the reagent compartment and dispenses them into the cuvette in the incubation ring. The reagent probe is washed with deionized water between aspirations to prevent carryover.4Step 4:  The cuvette incubates per the test protocol. Select Next to continue.     The sample and reagents then incubate for the appropriate time based upon the test protocol.5Step 5:  The cuvette is washed at the Wash Station. Select Next to continue.     After incubation, the cuvette moves to the wash station where magnets positioned along the incubation ring pull the magnetic particles to the side of the cuvette. Aspirate probes remove fluid from the cuvette and wash probes dispense Wash 1 into the cuvette to wash the particles. Multiple washes may be performed depending on the test protocol.6Step 6:  Acid is added to the cuvette. Select Next to continue.     The incubation ring moves to the cuvette to the acid probe. Here, acid reagent is dispensed to prepare for the chemiluminescent reaction.7Step 7:  The cuvette is lifted to the Luminometer. Select Next to continue.     The incubation ring moves to the elevator which lifts the cuvette up into the luminometer. The luminometer has three parts: a photomultiplier tube (PMT) to count photons, a base probe and a waste probe.8Step 8:  Base Reagent is dispensed and chemiluminescent flash occurs. Select Next to continue.The base probe dispenses base reagent into the cuvette to start the chemiluminescent reaction. The PMT measures the photons as RLUs (Relative Light Units) and converts the reading into a result.9Step 9:  The fluid is aspirated from the cuvette and waste is discarded.  When complete, select the X in the upper-right corner to close the window and continue.The waste probe aspirates fluid from the cuvette and dispenses it into the liquid waste container. The ejector rod pushes the cuvette out to the solid waste drawer. In the Incubation Ring window:  Boxes represent individual cuvette positions  Boxes with tests in progress contain test information such as the test name, sample ID and time to final result   Using the Incubation Ring Window Learn how to use the Incubation ring window to determine test information. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003367/sim_CP_IncRing800x600_9/sim_CP_IncRing800x600_9.swfHTML5 File:/content/generator/Course_90003367/sim_CP_IncRing800x600_9/index.htmlPDF File: To pause a sample run:      1.  Access the Sample Compartment screen      2.  Select Pause      3.  To resume sampling, select Resume The sample probe completes its current action and then stops. The reagent probe continues to dispense and all aspirated tests go to completion. Pause Learn about pausing a sample run. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003367/sim_CP_Pause800x600_9/sim_CP_Pause800x600_9.swfHTML5 File:/content/generator/Course_90003367/sim_CP_Pause800x600_9/index.htmlPDF File: To stop a sample run:      1.  Select Stop from the Task Bar      2.  Select OK from the emergency stop window Sample and Reagent probes continue their current action and then stop. Cuvettes that have sample and required reagents will result. Cuvettes that did not receive required reagents will be aborted. Stopping a sample Run simulation Learn about stopping a sample run. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003367/sim_CP_Stop800x600_9/sim_CP_Stop800x600_9.swfHTML5 File:/content/generator/Course_90003367/sim_CP_Stop800x600_9/index.htmlPDF File: