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ADVIA Centaur® XP Immunoassay System Loading Supplies and Reagents Online Training

This includes loading supplies and reagents and emptying waste to ensure efficient use of the ADVIA Centaur® XP system. This clinical laboratory training qualifies for continuing education units (CEU).

Welcome to the ADVIA Centaur® XP System Loading Supplies and Reagents Online Training course. In this course, the operator will learn how to perform pre-analytical procedures on the Centaur® XP system, including how to handle the following supplies: Acid and Base Reagent Wash 1 Water Cuvettes and Sample Tips Primary and Ancillary Reagents Liquid and Solid Waste Select Next to view the course objectives Describe how to empty waste containers Interpret the information provided on the Supplies Status window, the Reagent Status window, and the Reagent Inventory window List the steps to load supplies, primary reagents and ancillary reagents Recall reagent handling requirements After completing this course, you will be able to:   Select Next to continue. Congratulations. You have completed the ADVIA Centaur® XP Immunoassay System Loading Supplies and Reagents Online Training course. Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz.  In this online training course, you have learned to: Recall Reagent Handling requirements Primary and Ancillary reagents should be refrigerated, protected from light and stored upright. Interpret the information provided on the Supply Status window, Reagent Status window, and Reagent Inventory window  The Supply Status window keeps track of cuvettes, sample tips, Water, Wash 1, Acid and Base reagents and waste.  This window will notify the operator when supplies are running low and waste is 80% full. The Reagent Status window displays the Primary and Ancillary reagents that are currently loaded on the system. The Reagent Inventory window displays any reagent shortage on the system. List the steps to load supplies and reagents Supplies and reagents can be changed at any time. Cuvettes are loaded into the cuvette loading bin which can hold up to 1000 cuvettes. Sample Tips are loaded onto the Tip Loader.  Up to 840 tips can be loaded onto the system. Primary reagents should be properly mixed and then loaded into the Primary Reagent Compartment on the left side of the system. Ancillary reagents should be loaded into the Ancillary Reagent Entry. Acid and Base Reagent and Wash 1 are found in the System Fluids drawer in the front of the system. These fluids should be changed monthly or when the system notifies the operator that the bottle is running low. DI Water is located on the left side of the system in the Waste and Water Drawer. Empty liquid and solid waste containers The Cuvette Waste Bin and Tip Tray Waste are located on the left side of the system. The Tip Waste Bin is located under the Ancillary Reagent Entry. Liquid Waste is located on the left side of the system in the Waste and Water drawer. Select Next to continue.   Supplies Status Changes yellow to indicate a supply is running low or waste is 80% full Blinks red if a supply is empty or waste is completely full Reagent Status Blinks yellow to indicate that a reagent pack cannot be used Reagent Inventory Status Displays available tests for the current sample worklist Supplies, Reagent Status and Reagent Inventory Learn about the supplies status, reagent status and reagent inventory windows. Tab TitleTextSupplies Status Supplies Status At the workspace, the Supplies Status button changes color to indicate the status of system supplies. The supplies status monitors the levels of cuvettes, sample tips, system fluids and waste.  A yellow alert indicates that the system is using the reserve; a supply is running low or waste is 80% full.  A red alert indicates a supply and its reserve are depleted or the waste and its reservoir are full.   Select the Supplies Status button to open the Supplies Status window to determine which supply needs attention.  The system will not start if the Supplies Status button is yellow and the system status is Check Status.   Reagent Status Reagent Status The reagent status monitors the status of Primary and Ancillary reagents on the system.  Any reagent that cannot be used is highlighted in yellow. The Reagent Status button changes to yellow to indicate that a reagent pack cannot be used.  Select the Reagent Status button to open the status window of the reagent that requires attention.   Reagent Inventory Reagent Inventory The Reagent Inventory window compares the current worklist orders to the reagents available on the system. It will alert the operator if the reagent supply is insufficient. This window displays the number of tests scheduled, the available tests on the system and the number of tests the system will need to complete the run. When complete, select the X in the upper-right corner to close the window and continue.   Status Windows Practice identifying the status windows. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003241/sim_CXP_StatusWindows800x622_9/sim_CXP_StatusWindows800x622_9.swfHTML5 File:/content/generator/Course_90003241/sim_CXP_StatusWindows800x622_9/index.htmlPDF File: Supplies and Reagents include: Cuvettes Sample Tips Acid and Base reagent Wash 1 Water Loading Supplies Learn about management of supplies and reagents. Checklist TitleChecklist TypeChecklist ContentObserve Message: No Cuvettes DetectedHTML The cuvette supply is depleted or an error occurred during cuvette loading and the system stopped sampling. If the system has run out of cuvettes, the Supplies Status window will flash red.  When you open the Supplies Status window, the cuvette field will display a “No Cuvette Detected” message.  Once you have replenished the cuvette supply, select the Prime Cuvettes button at the bottom of the Supplies Status window. Select each checkbox to learn more about managing supplies and reagents.  Load CuvettesHTML  Cuvettes may be loaded at any time. The cuvette loading bin holds up to 1000 cuvettes or 5 bags. Select the play arrow to begin the video.  Observe Message: HTML If the sample tip supply is running low on the system, the Supplies Status button will flash yellow.  When you open the Supplies Status window, the Tips field will display a "<120 tips" message, indicating there are less than 120 tips (1 tray) available.  Load Sample TipsHTML   Sample Tips can be loaded at any time. Each tip tray holds 120 tips.  Up to 840 tips can be loaded onto the system. Select the play arrow to begin video.  Load Water as NeededHTML   The deionized water bottle should be replenished as needed.  The bottle can be removed from the system at any time.  The capacity of the water bottle is 10L. Note: This procedure is not required if the system is directly plumbed for water. Select the play arrow to begin the video.  Observe Message: Acid & Base Present,Using ReserveHTML If the system is running low on acid and base, the Supplies Status window will flash yellow.  When the Supplies Status window is opened, the Acid and Base fields will display a “Present, Using Reserve” message. This means that the acid and/or base bottle is empty and the system is using the reserve.  Replace Acid and Base ReagentHTML   Acid and base reagents should be replaced when any of the following conditions exist: The reagent has been on the system for a month The reagent has expired The reagent has insufficient volume Note: Replace acid and base reagents at the same time to manage lot numbers and to obtain optimum reagent performance. They can be replaced at any time. Select the play arrow to begin the video.  Observe Message: Wash 1 Present, Using ReserveHTML If the Wash 1 supply is running low, the Supplies Status button will flash yellow. When you open the Supplies Status window, the Wash 1 field will have a “Present, Using Reserve” message indicating that the bottle is empty and the system is using the reserve.  Replace Wash 1HTML   Wash 1 should be replaced when any of the following conditions exist: The reagent has been on the system for a month The reagent has expired The reagent has insufficient volume This bottle can be replaced at any time.  Select the play arrow to begin video.  Observe Status MessagesHTML Right-click on the supply field to obtain a description of the status messages and the procedure for replenishing each supply.    When complete, select the X in the upper-right corner to close the window and continue.      Primary Reagents: Mix by hand to resuspend the paramagnetic particles of the Solid Phase reagent Load the primary reagent pack into the primary reagent compartment after mixing If you remove reagents from the primary reagent compartment, cover the pierced area with self-sealing laboratory film Use caution when mixing pierced primary reagent packs to avoid spills Note: CEA, aHBs and H2n require special primary reagent handling.  Refer to Instructions for Use for more details. Ancillary Reagents: Mix all ancillary reagent packs by hand before loading them onto the system Load ancillary reagents onto the ancillary reagent entry area If ancillary reagents are removed from the system, cover the pierced area with self-sealing laboratory film Use caution when mixing pierced ancillary reagent packs Note: Certain methods require ancillary preparation. Refer to Instructions for Use for more details. Storage of reagents: To properly store reagents: Protect them from light, keep refrigerated, and keep upright. Reagent Handling Learn about mixing and loading reagent packs. Checklist TitleChecklist TypeChecklist ContentMix Primary ReagentHTML   Mix primary reagents before loading: Raise one end of the pack to a 90° angle and return to a horizontal position Raise the other end of the pack to a 90° angle and return to a horizontal position Repeat at least 20 times or until the paramagnetic particles are no longer visible on the bottom of the pack Special mixing required for CEA, aHBs and Her-2/neu: Shake back and forth vigorously for 15 seconds Tap the pack on the bench 5 times Select the play arrow to begin the video. Select each checkbox to learn how to mix and load reagent packs. Mix Pierced Primary Reagent PacksHTML To mix pierced primary reagent packs: Cover the pierced area of the pack Raise one end of the pack to a 45° angle and return to a horizontal position Raise the other end of the pack to a 45° angle and return to a horizontal position Repeat for a minimum of 20 times or until the paramagnetic particles are no longer visible on the bottom of the pack  Load Primary Reagent PacksHTML   Loading Primary Reagent Packs: Open the primary reagent compartment and identify a position for the pack. Pull the reagent holder toward you and load the pack. Push the reagent holder back. Close the primary reagent compartment door. For optimal throughput, install packs of similar types next to one another.  Similar types of reagents are identified by the color on the end of the label. Purple – load packs from the left side towards the center of the primary reagent compartment Orange – load packs from the right side towards the center of the primary reagent compartment Green – load packs between any purple and orange packs Select the play arrow to begin the video. Remove Primary Reagent PacksHTML Removing Primary Reagent Packs: Open the Primary Reagent Status window. Select the reagent pack. Select Remove Pack. If prompted, select Complete or Now. When the status changes to Removable and the LED changes to neutral, remove the reagent pack. Note: Do not remove a primary pack when the system is in process if the LED on the primary reagent door is green.   Load Ancillary Reagent PacksHTML   Loading Ancillary Reagent Packs: Gently invert the pack several times. Place the reagent pack in the ancillary reagent entry with the barcoded side facing outward. An LED next to the ancillary reagent entry turns green to indicate that the pack is loading. The system moves the pack into the ancillary reagent compartment. Select the play arrow to begin the video.   Remove Ancillary Reagent PacksHTML Removing Ancillary Reagent Packs: Open the Ancillary Reagent Status window. Select the ancillary pack. Select Remove Pack. If prompted, select Complete or Now. The system ejects the pack into the ancillary reagent entry. An LED next to the ancillary reagent area turns orange to indicate that the pack is exiting the system. When complete, select the X in the upper-right corner to close the window and continue. The types of waste containers include: Cuvette waste Tip waste Tip Tray waste Liquid waste Note: Refer to the ADVIA Centaur® XP User Interface Online Training course for additional details on the Supplies Status window. Empty Waste Containers Learn about the procedure for emptying waste containers. Checklist TitleChecklist TypeChecklist ContentCuvette WasteHTML If the Supplies Status window indicates that the cuvette waste is >80% full, empty the cuvette waste to ensure that your system does not stop processing.   Select each checkbox to learn more about emptying waste containers.    Empty Cuvette WasteHTML Open the door on the left of the system and pull out the cuvette waste bin to empty. Return the bin to the system and close the cuvette waste area door.  Select YesHTML On the workspace, acknowledge the popup.  If the cuvette waste was emptied, select Yes.    Note:  Do not confirm waste disposal if the bin was not actually emptied.  Cuvette jams may occur if you respond incorrectly.  Tip WasteHTML If the Supplies Status window indicates that the tip waste is >80% full, empty the tip waste bin to ensure that your system does not stop processing.  Empty Tip WasteHTML Open the door under the Ancillary Reagent Entry and pull out the tip waste bin to empty it.   Return the bin to the system and close the door.  Select YesHTML Respond appropriately to the pop-up window.  If you emptied the tip waste, select Yes.    Note:  Do not confirm waste disposal if the bin was not emptied.  Tip jams may occur if you respond incorrectly.  Tip Tray WasteHTML If the Supplies Status window indicates that the tip tray waste is >80% full, empty the tip tray waste.  Empty Tip Tray WasteHTML Open the door on the left of the system and remove the tip trays and covers.   Close the door.  Select YesHTML On the workspace, respond to the popup.  Select Yes if the tip tray waste was emptied.  Liquid WasteHTML If the Supplies Status window indicates that the liquid waste is full and using the reserve, empty the liquid waste bottle as soon as possible to avoid system errors.  Remove Liquid WasteHTML Pull out the waste and water drawer to lift the waste lid.  Remove the tubing quick disconnect and glass rod sensor.  Insert the Waste Bottle Stopper into the openings to prevent spillage. Note: This procedure is not required if the system is directly plumbed for waste.    Dispose of Liquid WasteHTML Use the spigot to dispose of liquid waste in accordance with local, state and federal regulations. Return the bottle to the waste and water drawer, remove the Waste Bottle Stopper and replace the quick disconnect tubing.  Wipe the glass rod sensor with a dry lint-free tissue and replace it on the top of the bottle.  Close the lid and push the waste and water drawer into its proper place.  Select ContinueHTML Select Continue to confirm that the waste tubing has been properly attached to the bottle.   When complete, select the X in the upper right corner to close the window and continue.