ADVIA® Chemistry XPT System Calibration Online Training

Welcome to the ADVIA® Chemistry XPT System Calibration Online Training course. In this course you will learn how to process the following types of calibrations: Reagent blank Single point Multi-point ISE (ion selective electrode) Select Next to continue. Upon successful completion of this course, you will be able to: Describe the process to review calibration status Describe how to add and edit a calibrator definition List the steps required to run a calibration for chemistry assays List the steps required to run a calibration for ISE assays Describe how to monitor the status of in progress calibrations Describe how to review calibration data Select Next to continue. Congratulations.  You have completed the ADVIA® Chemistry XPT System Calibration Online Training course.  Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz. Select this link to download and print a copy of the Course Review. Describe the process to review calibration status Calibration on the ADVIA Chemistry XPT System is required in the following instances: The calibration interval expires for an assay There is a change in the reagent lot number After a lamp change If warranted by QC To check calibration status, access the Reagent Inventory screen by selecting Reagents from the Command Bar, and then selecting the Inventory tab. The Reagent Inventory screen displays information for all of the reagents onboard the system, including the calibration status and calibration interval. Describe how to add and edit a calibrator definition Use the Calibrator Definitions screen to add the following to the system: Add new calibrator and reagent blank definitions Edit existing calibrator and reagent blank definitions To access the Calibrator Definitions screen, select Calibration from the Command Bar, and then select the Calibrator Definitions tab. List the steps required to run a calibration for chemistry assays Reagent Blank (RBL): All chemistry assays on the ADVIA Chemistry XPT System require that a reagent blank is run along with the calibrator(s) as part of the calibration process Some assays, such as certain enzymatic assays, require that only the reagent blank be performed during calibration Information in the IFU (Instructions for Use) for a particular assay may allow the operator to perform a reagent blank at a certain time to extend or correct the calibration interval for that assay. That is considered a partial calibration, and is only performed after a full valid calibration is stored for that assay. Types of Chemistry Assay Calibrations: Reagent Blank Uses only water RBL for calibration of enzyme assays Single Point Uses a single calibrator (water RBL + calibrator) Multipoint Uses multiple levels of calibrators (matrix/Level 1 calibrator RBL + the other calibrators in the kit) NOTE: RBL for a multipoint calibration uses a matrix or Level 1 calibrator instead of water Prior to using a calibrator on the system, you must ensure that the appropriate calibrator is: The intended calibrator for that assay Prepared according to the calibrator preparation procedure listed in the calibrator package insert Loaded on the system in the appropriate position After the calibrator samples are loaded on the system, you may program and initiate the calibration run. Note: Calibrators for different types of assays (absolute, single point, multipoint) may be programmed and run simultaneously along with appropriate quality control material. To program chemistry calibrations: Select System Operations from the Command Bar Select Start/Restart Select Analyze Single Point and/or Analyze Multipoint Select Assay Selection Select the assays to be run Select OK twice Select Yes List the steps required to run a calibration for ISE assays The ADVIA XPT Chemistry System processes three tests through Ion Selective Electrodes, or ISE methodology: Sodium Potassium Chloride ISE Calibrators are: In liquid form and stored at room temperature Available for serum and urine calibration Designated positions on the CTT After the ISE calibrator samples are loaded on the system, you may program and initiate the calibration run. ISE calibrations can be programmed from two Command Bar options: System Operations Maintenance (also includes option to perform a Buffer Prime) To program an ISE calibration using the Maintenance area of the software: Select the Maintenance button from the Command Bar Select the ISE Operations tab Perform a Buffer Prime Select Calibration  Make any necessary changes to the settings Select Perform Describe how to monitor the status of in progress calibrations Once the calibration run has been initiated, the status of the calibration progress can be monitored on the Sample Trays screen: Select Samples from the Command Bar, and then select the Sample Trays tab Displays a diagram of the sample tray (STT) and calibration tray (CTT) Has a color legend which is used to determine sample processing status Displays the amount of time left for processing  by selecting one of the sample positions Can search for a sample by Sample ID or Sample Position Describe how to review calibration data After the calibration is completed, the calibration status may be viewed by accessing the Calibration button on the Command Bar.  Within Calibration, there are three screens that display calibration status: Calibration Results ISE Chemistry If the calibration is successful, the calibration status column on these screens will display "Pass". Select Next to continue. Calibration on the ADVIA® Chemistry XPT System is required in the following instances: The calibration interval expires for an assay There is a change in the reagent lot number Certain maintenance activities are performed, such as after a lamp change If warranted by QC Check Calibration Status Learn how to check calibration status.  Access the Reagent Inventory screen by selecting Reagents from the Command Bar, and then selecting the Inventory tab. The Reagent Inventory screen displays information for all of the reagents onboard the system, including the calibration status and calibration interval. When complete, select the X in the upper-right corner to close the window and continue. Use the Calibrator Definitions Screen to add the following to the system: Add new calibrator and reagent blank definitions Edit existing calibrator and reagent blank definitions NOTE: Operators must be logged into the system at the Laboratory Manager level to add and edit calibrator definitions.   Calibration Definition Learn how to add a calibration definition. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90013780/sim_ChemXPT_AddingCalibratorDefinitions_HTML5_9/sim_ChemXPT_AddingCalibratorDefinitions_HTML5_9.swfHTML5 File:/content/generator/Course_90013780/sim_ChemXPT_AddingCalibratorDefinitions_HTML5_9/index.htmlPDF File: Reagent Blank (RBL): All chemistry assays on the ADVIA® Chemistry XPT System require that a reagent blank is run along with the calibrator(s) as part of the calibration process Some assays, such as certain enzymatic assays, require that only the reagent blank be performed during calibration Information in the IFU (Instructions for Use) for a particular assay may allow the operator to perform a reagent blank at a certain time to extend or correct the calibration interval for that assay. That is considered a partial calibration, and is only performed after a full valid calibration is stored for that assay. Types of Chemistry Assay Calibrations: Reagent Blank Uses only water RBL for calibration of enzyme assays Single Point Uses a single calibrator (water RBL + calibrator) Multipoint Uses multiple levels of calibrators (matrix/Level 1 calibrator RBL + the other calibrators in the kit) NOTE: RBL for a multipoint calibration uses a matrix or Level 1 calibrator instead of water Select Next to continue. Prior to using a calibrator on the system, you must ensure that the appropriate calibrator is: The intended calibrator for that assay Prepared according to the calibrator preparation procedure listed in the calibrator package insert Loaded on the system in the appropriate position   Calibrator Tray Position Learn how to determine the calibrator tray position. To determine the container type of tray position for calibrators and water blank: Select Samples from the Command Bar Select the Cal/QC Sample Settings tab   Calibrator or Water Blank Tray Selection Single Point CTT Multipoint MSTD 1 or MSTD 2 (both on STT) Reagent Blank CTT When complete, select the X in the upper-right corner to close the window and continue. After the calibrator samples are loaded on the system, you may program and initiate the calibration run. Note: Calibrators for different types of assays (reagent blank, single point, multipoint) may be programmed and run simultaneously.   Program Reagent Blank Calibration Learn how to program a reagent blank calibration. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90013780/sim_ChemXPT_ProgrammingAbsoluteCalibrations_HTML5_9/sim_ChemXPT_ProgrammingAbsoluteCalibrations_HTML5_9.swfHTML5 File:/content/generator/Course_90013780/sim_ChemXPT_ProgrammingAbsoluteCalibrations_HTML5_9/index.htmlPDF File: Program Single Point Calibration Learn how to program a single point calibration. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90013780/sim_ChemXPT_ProgrammingSinglePointCalibrations_HTML5_9/sim_ChemXPT_ProgrammingSinglePointCalibrations_HTML5_9.swfHTML5 File:/content/generator/Course_90013780/sim_ChemXPT_ProgrammingSinglePointCalibrations_HTML5_9/index.htmlPDF File: Multipoint Calibration Learn how to program a multipoint calibration. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90013780/sim_ChemXPT_ProgrammingMultiplePointCalibrations_HTML5_9/sim_ChemXPT_ProgrammingMultiplePointCalibrations_HTML5_9.swfHTML5 File:/content/generator/Course_90013780/sim_ChemXPT_ProgrammingMultiplePointCalibrations_HTML5_9/index.htmlPDF File: The ADVIA® XPT Chemistry System processes three tests through Ion Selective Electrodes, or ISE methodology: Sodium Potassium Chloride Select Next to continue. ISE Calibrators are: In liquid form and stored at room temperature Available for serum and urine calibration Designated positions on the CTT:   CTT Position ISE Calibrator 11 ISE Serum Calibrator Low 12 ISE Serum Calibrator High 13 ISE Urine Calibrator Low 14 ISE Urine Calibrator High Select Next to continue. After the ISE calibrator samples are loaded on the system, you may program and initiate the calibration run. ISE calibrations can be programmed from two Command Bar options: System Operations Maintenance (also includes option to perform a Buffer Prime)   ISE Calibration Learn how to program an ISE calibration. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90013780/sim_ChemXPT_ProgrammingISECalibrations_HTML5_9/sim_ChemXPT_ProgrammingISECalibrations_HTML5_9.swfHTML5 File:/content/generator/Course_90013780/sim_ChemXPT_ProgrammingISECalibrations_HTML5_9/index.htmlPDF File: Once the calibration run has been initiated, the status of the calibration progress can be monitored on the Sample Trays screen: Select Samples from the Command Bar, and then select the Sample Trays tab Displays a diagram of the sample tray (STT) and calibration tray (CTT) Has a color legend which is used to determine sample processing status Displays the amount of time left for processing  by selecting one of the sample positions Can search for a sample by Sample ID or Sample Position   Sample Trays Screen Learn how to navigate the Sample Trays screen. Base ImageHotspotsText BlocksImage FileThe position and sample identification code (SID) of the last sample aspirated is displayed.The Sample Information area includes the Sample ID, Position, Status, and Estimated Time of Arrival (ETA) for the sample that is currently selected on the STT or CTT diagram. The ETA is the point at which the processing of the sample is complete. If the selected sample is a barcode analysis, the barcode number displays, and the Position number is 0.The STT is represented by the two outermost rows of circles. Each numbered circle represents a sample position on the STT. As the samples are processed, the numbered circles change color to indicate the current status.The CTT is represented by the two innermost rows of circles. Each numbered circle represents a sample position on the CTT. As the samples are processed, the numbered circles change color to indicate the current status.To search for a sample, enter the Sample ID, Tray, or Cup, and then select Search.The Legend defines the sample status for each color. After the calibration is completed, the calibration status may be viewed by accessing the Calibration button on the Command Bar.  Within Calibration, there are three screens that display calibration status: Calibration Results ISE Chemistry If the calibration is successful, the calibration status column on these screens will display "Pass". Calibration Results Screens Learn about the screens used to view calibration results. Slide NumberText BlocksCalloutsAudio ScriptImage File1Calibration Results To access, select Calibration > Calibration Results Displays general information about ISE and chemistry calibration events. Also used to do the following tasks: Access the Calibration Order Filter Access the Calibration Results View window Delete calibration results Print Calibration Results Reports Select Next to continue. Note: If audio does not automatically start, select the play arrow in the top left to begin.Select each number to review the corresponding text.CalloutsCalibration Order FilterCalibration Results View windowDelete calibration resultsPrint Calibration Results ReportsTo access, the Calibration Results screen, first select Calibration from the Command Bar, and then select the Calibration Results tab. The Calibration Results screen displays general information about ISE and chemistry calibration events. This screen is also used to do the following tasks: Access the Calibration Order Filter, Access the Calibration Results View window, Delete calibration results, and Print Calibration Results Reports. Select Next to continue. 2ISE To access, select Calibration > ISE Displays general information about ISE calibrations. Also used to do the following tasks: Access the ISE Calibration Filter Access the ISE Historical Graph window Print ISE Monitor Reports Select Next to continue. Select each number to review the corresponding text.CalloutsISE Calibration FilterISE Historical Graph windowPrint ISE Monitor ReportsTo access the ISE screen, first select Calibration from the Command Bar, and then select the ISE tab. The ISE screen displays general information about ISE calibrations. This screen is also used to do the following tasks: Access the ISE Calibration Filter, Access the ISE Historical Graph window, and Print ISE Monitor Reports. Select Next to continue. 3Chemistry To access, select Calibration > Chemistry Displays general information about chemistry calibrations, including the status and date and time of the most recent calibration. Also used to do the following tasks: Filter chemistry calibrations View Calibration Detail Reset chemistry calibrations Waive chemistry calibrations Print Calibration Chemistry Reports When complete, select the X in the upper-right corner to close the window and continue.Select each number to review the corresponding text.CalloutsFilter chemistry calibrationsView Calibration DetailReset chemistry calibrationsWaive chemistry calibrationsPrint Calibration Chemistry Reports To access, the Chemistry screen, select Calibration from the Command Bar, and then select the Chemistry tab. The Chemistry screen displays general information about chemistry calibrations, including the status and date and time of the most recent calibration. This screen is also used to do the following tasks: Filter chemistry calibrations, View Calibration Detail, Reset chemistry calibrations, Waive chemistry calibrations, and Print Calibration Chemistry Reports. When complete, select the X in the upper-right corner to close the window and continue.