BN™ II System Software and Basic Operations Online Training

Upon successful completion of this course, you will be able to: Identify the BN™ II System software and operating system   Describe the layout of the Info dialog   Identify the functions of the software's six commonly used dialogs Describe how to perform basic operations Select Next to continue. The BN™ II System software runs on an APPLE MACINTOSH operating system. On the desktop, you'll see icons for the Macintosh HD and the BN™ II Alias, indicating that the hard drive has been partitioned or split. Some systems are not partitioned and will have only one icon. All of the files pertaining to the BN™ II are on the BN™ II hard drive. To launch the applications software, just double-click the BN™ II icon.   The first dialog that appears after start-up is the Info dialog, the main dialog of the BN™ II System applications software. You can't close this dialog—if you open another dialog, the Info dialog is always open in the background. What's the overall status of the analyzer? Check the color of the Info dialog's background! Green means that everything is okay Yellow means that there is a need, but the analyzer can continue to process Red means that there is a critical need or the analyzer has stopped processing The background color always matches the color of the display area with the greatest need. If one of the display areas has a red status, then the background of the Info dialog will be red. In the Info dialog shown here, the background is green, indicating that everything is okay.   Info Dialog Learn about the Info dialog by selecting the image areas. Base ImageHotspotsText BlocksImage FileDisplay areas—Six display areas that give you information about the system. Each row acts as an active button that you can click to open another dialog with more information.Menu bar—Contains menus with commands and submenus that you use to perform tasks and operations. Even the Apple has a menu!Status display—Displays the current color status of the system and additional miscellaneous information.Toolbar—Contains six icons that act as shortcuts to areas or functions that you'll access frequently. Simply roll your mouse anywhere in the toolbar to view the names of the icons. Use the Loading dialog to manually identify an item loaded on the analyzer and to assign positions for non-barcoded samples. You'll learn more about this dialog when we practice processing samples later in this course.   Print and Close Dialogs Learn about printing and closing dialogs.   Every dialog has its own toolbar (solid arrow). The icons on this toolbar differ somewhat across dialogs, but you'll find that two icons—the Printer and Close icons—are always the same. To print a dialog, click the Printer icon (circled in blue) To close a dialog, click the Close icon (circled in red) or click the close box in the title bar (dashed arrow) Use the Rack status dialog to view the contents of a rack and to eject racks. It shows a graphic of the 15 rack lanes in the rack unit. To view a rack's contents, simply click the rack. The rack ID, the lane number, and a list of the rack’s contents are displayed in the list box below. To eject racks, use the two buttons on the right (see inset): To eject a single rack, select that rack and click the right button. The rack will be removed from the processing area To eject ALL racks, click the left button. All of the racks will be removed one by one, so be sure that’s what you really want to do   Use the Lab journal dialog to view sample IDs and the requested tests for each sample (called the job list), as well as the results for your samples. This dialog displays symbols and flags that indicate processing status, problems, or information for a sample or result. We'll take a closer look at this dialog when we process samples later in this course.   The Patient Journal dialog displays only samples for which patient demographics have been entered. Use this dialog if you want to print reports showing patient names, birth dates, and results, as well as reference ranges. All of these samples are also displayed in the Lab journal dialog, which is the one that we'll use for this training course.     Use the Control journal dialog to view names of controls, assays requested, and control results. We’ll take a detailed view of this dialog when we process a control later in this course.   Use the Reference curves dialog to request the measurement of a calibration curve, cancel a request, and view the number of stored curves for each assay. We’ll use this dialog when we process a calibration later in this course.   The central area of the Info dialog has six display areas—Requests, Samples, Reagents, Validation, Analyzer, and Information. Each row in a display area acts as a button that you can click to open another dialog with more information. You'll have a chance to practice using this feature later in this course.   Display Area Details Learn about key details of the display areas. There's a lot of information packed into each display area. Let's go over the details. Title Bar Every display area has a title bar (solid arrow) that shows the name of one functional area of the system. Here, the Samples display area is shown—all of the information in this display area pertains to the processing of samples. Status Indicators Some rows have square indicators (dashed arrows). The color of the indicator tells you the status of that part of the functional area. Green, yellow, and red have the same meanings as they have for the Info dialog background What's the overall status of an area? Look at the color surrounding the title (solid arrow). It reflects the status of the row that has the greatest need. The color is yellow here because yellow indicates a greater need than green     Display Areas Learn about each display area. Slide NumberText BlocksCalloutsAudio ScriptImage File1The Requests display area shows a numerical summary of the status of tests requested. Number: Total number of tests requested in the current job list (the list of samples and the tests requested on each that is displayed in the Lab journal dialog) released: Number of results that have been released to the host computer not released: Number of results that have not been released to the host computer in process: Number of requests currently being processed waiting: Number of requests not yet processed because the samples have not been loaded remeasurements: Number of repeat measurements requested or performed automatically Enter your script here.2The Samples display area shows a numerical summary of the status of samples. Number: Total number of samples in the current job list finished: Number of samples for which results are available and processing is completed loaded: Number of samples on the analyzer not sufficient: Number of samples with volume that is insufficient to process test requests missing: Number of samples not loaded on the analyzer without req.: Number of samples without requests that are loaded on the analyzer Enter your script here.3The Reagents display area shows a numerical summary of the status of reagents, as well as that of standards and controls. Reagents: Number of reagent vials on the analyzer missing: Number of reagent vials required for processing, but not on the analyzer or empty not sufficient: Number of reagent vials with insufficient volume to process the complete job list not required: Number of reagent vials that are on the analyzer but not needed STDS/CTRLS: Number of standard and control vials on the analyzer missing: Number of standard and/or control vials required for processing, but not on the analyzer or empty not sufficient: Number of standard and/or control vials with insufficient volume to process the complete job list not required: Number of standard and/or control vials on the analyzer but not needed                       Enter your script here.4The Validation display area shows a numerical summary of the status of reference (calibration) curves and the invalid controls. Reference: Total number of reference curves measured and in process OK: Number of valid reference curves (a curve that is acceptable according to the specified mean deviation) not ok: Number of invalid reference curves in process: Number of reference curves that are being measured currently missing: Number of reference curves required for the sample or control results to be determined Assays: invalid contr.: Number of controls whose results lie outside the range of confidence (the acceptable range for a control defined as a certain percentage deviation on each side of the control mean) Enter your script here.5The Analyzer display area shows information about four critical areas of the BN™ II System. Diln. wells: Total number of dilution wells on the analyzer as entered by the operator available: Number of new (unused) dilution wells missing: Number of dilution wells not available that are needed to complete the requests in the job list System liquids: Status of all system liquids (buffer, diluent, and wash solution)—full, low, or empty Cuvettes: Total number of cuvettes in the cuvette rotor OK: Number of cuvettes with acceptable blank readings Connection: Status of the connection between the computer and the analyzerEnter your script here.6The Information display area shows additional information about messages, processing, and the system status, and it has a button for adjusting the dialog that is foremost on the screen. Logbook: Number of new messages in the logbook that have not been addressed Measurement: Number of minutes until processing is completed for the current job list or the message "finished" System status: Message indicating the current mode or status of the analyzer Send behind: If a dialog is open in the background, click this button to send the Info dialog to the background, which brings the open dialog to the foregroundEnter your script here. Now that you've had an overview of the software, let’s prepare the analyzer by starting up the system and performing the four daily maintenance tasks: Check the system liquids and replace them if needed Empty the waste container Replace the used dilution strips Inspect the components of the dispensing unit You can perform some of these tasks before you turn the analyzer on.     Empty Waste Container Learn about emptying the waste container. Checklist TitleChecklist TypeChecklist ContentInspect the Waste ContainerHTML   If you're using an external waste container, you'll need to visually monitor the level and empty the waste periodically to ensure that it has ample space for the 2.5 L that the internal waste container holds.     Identify the Waste Container LinesHTML   The waste container has two lines: One line is attached to the analyzer's waste outlet. Fluid in this line comes from the internal waste container The other line is attached to the analyzer's air outlet. It is the air exhaust from the vacuum pump and it contains a small amount of condensation Empty the Waste ContainerHTML   Remove the lines, empty the waste container, and then replace the lines in the container.     Preparing the Analyzer Learn about preparing the analyzer. Instructions:Flash File:/content/generator/Course_90017383/sim_BNII_SBO_PreparingtheAnalyzer_HTML5/sim_BNII_SBO_PreparingtheAnalyzer_HTML5.swfHTML5 File:/content/generator/Course_90017383/sim_BNII_SBO_PreparingtheAnalyzer_HTML5/index.htmlPDF File: There's one preparation step before processing calibrations and controls—you must enter assigned values for the standards and controls into the software. Fortunately, you don't have to enter these values manually--you can simply scan them in using the barcode wand. Note: To enter assigned values, refer to the package insert sheet provided with all standard and control products. These sheets give the assigned values in two formats—numerals and barcodes You can enter the numerical values manually. Or you can scan the lines of barcode with the barcode wand to enter the values automatically For detailed instructions, refer to your BN™ II System Operator's/Instruction Manual Place the insert sheet on a flat surface, then just start scanning the lines of barcode designated for the BN™ II System. When you’ve scanned the first line, the Scan barcode dialog appears automatically. You must scan all the lines, but you can scan them out of order, from left to right, or right to left, and the system knows if you scan the same line twice. When you have scanned them all, the Scan barcode dialog closes automatically and the values are stored.     You must establish a calibration (reference) curve for each assay in order to measure the concentrations of proteins in controls and samples. To do this, you'll process standards that have known concentrations of analytes for that assay. When do you need to calibrate? When you begin using a new lot of reagent When your quality control results have significantly shifted within the range of confidence or are outside of this range Let’s practice calibrating a reagent lot.   Processing Calibration Learn about processing calibrations. Instructions:Flash File:/content/generator/Course_90017383/sims_BNII_SBO_processingcalibrations_HTML5/sims_BNII_SBO_processingcalibrations_HTML5.swfHTML5 File:/content/generator/Course_90017383/sims_BNII_SBO_processingcalibrations_HTML5/index.htmlPDF File: Run quality control (QC) to ensure the accuracy and precision of analytical results produced by the BN™ II System. When should you process controls? After calibrating a reagent Periodically, to check the performance of the system A successful QC run indicates that your calibration curve is valid, the system is working well, and samples can be analyzed. Let’s practice processing controls.   Processing Controls Learn about processing controls. Instructions:Flash File:/content/generator/Course_90017383/sims_BNII_SBO_ProcessingControlsRev/sims_BNII_SBO_ProcessingControlsRev.swfHTML5 File:/content/generator/Course_90017383/sims_BNII_SBO_ProcessingControlsRev/index.htmlPDF File: The BN™ II System can process samples of serum, plasma, urine, and cerebrospinal fluid. The rack unit can hold up to 100 samples at a time! You can use a variety of primary and secondary sample tubes, including 3-, 5-, 7-, and 10-mL sizes. And you can process samples in both barcoded and non-barcoded tubes, although the steps for processing differ a bit. Let's take a closer look at these tasks.     Performing periodic maintenance procedures is critical to keeping your BN™ II System running smoothly. Most of these procedures are menu driven and can be accessed with the System menu. Let’s review our daily maintenance tasks and look at those required weekly, monthly, and yearly. For detailed instructions on each task, refer to your BN™ II System Operator's/Instruction Manual. Daily Maintenance Learn about performing daily maintenance. Checklist TitleChecklist TypeChecklist ContentInspect the dispensing unitHTML   Inspect the tubing, syringes, and syringe valves in the dispensing unit Replace used dilution stripsHTML   Replace used dilution strips Check/replace the system liquidsHTML   Check/replace the system liquids Empty the waste containerHTML   Empty the waste container Update your maintenance logHTML    Update your maintenance log Weekly Maintenance Learn about performing weekly maintenance. Checklist TitleChecklist TypeChecklist ContentClean instrument surfaces and rack lanesHTML   Clean instrument's surfaces, rotor cover, trough of the dilution unit and rack lanes with 70% alcohol Clean the probes of the two transfer armsHTML   Clean the probes of the two transfer arms. Back up the Data folderHTML   Back up the Data folder Update your maintenance logHTML   Update your maintenance log Monthly Maintenance Learn about performing monthly maintenance. Checklist TitleChecklist TypeChecklist ContentDecontaminate the wet systemHTML   Decontaminate the wet system with Neodisher GK® Replace the wash filterHTML   Replace the wash filter in the wash solution tubing Replace the reaction cuvettesHTML   Replace the reaction cuvettes Replace the wash solution containerHTML   Replace the wash solution container Clean the level sensorsHTML   Clean the level sensors (cannulae) with moist cloth Clean the barcode scannerHTML   Clean the barcode scanner with 70% alcohol solution Clean the mouseHTML   Clean the mouse Update your maintenance logHTML   Update your maintenance log Yearly Maintenance Learn about performing yearly maintenance. Checklist TitleChecklist TypeChecklist ContentReplace the syringesHTML    Replace the syringes Update your maintenance logHTML   Update your maintenance log Congratulations! You’ve completed the BN™ II System Software and Basic Operations course. In this online training course, you’ve learned how to: Identify the operating system and the desktop icons You’ve learned that the BN™ II System runs on an APPLE MACINTOSH operating system. And now you can identify the BN™ II icon on the desktop, as well as other icons.   Describe the layout of the Info dialog, the software's main dialog Now you know that the Info dialog has a menu bar and a toolbar that you use to navigate the software and perform tasks, a Status display, and—most importantly—six display areas that provide a wealth of information about the status of the system at a glance.   Identify the functions of the software's six commonly used dialogs You've learned that the software has six commonly used dialogs—the Loading, Rack status, Lab journal, Patient journal, Control journal, and Reference curves dialogs—each of which has important functions.   Describe how to perform basic operations using the software You’ve learned steps for preparing the analyzer and for processing calibrations, controls, and both barcoded and non-barcoded samples. And now you know generally how to perform maintenance tasks to keep your BN™ II System running smoothly.     What are the advantages of processing your samples in barcoded tubes? Barcoded samples offer not only positive sample identification, but also ease of use for your laboratory. You can process barcoded samples both: When the system is interfaced to a host computer (online) When the system is not interfaced (offline) Let's practice processing barcoded samples both ways.     Barcoded Samples Learn about processing barcoded samples. Instructions:Flash File:/content/generator/Course_90017383/sims_BNII_SBO_processingbarcodedsamples_HTML5/sims_BNII_SBO_processingbarcodedsamples_HTML5.swfHTML5 File:/content/generator/Course_90017383/sims_BNII_SBO_processingbarcodedsamples_HTML5/index.htmlPDF File: When do you use the procedure for processing non-barcoded samples? When your sample tubes do not have barcode labels When you use the special rack for pediatric or low-volume samples   Let's practice processing non-barcoded samples.   Non-Barcoded Samples Learn about processing non-barcoded samples. Instructions:Flash File:/content/generator/Course_90017383/sims_BNII_SBO_ProcessingNonbarcodedSamplesRev/sims_BNII_SBO_ProcessingNonbarcodedSamplesRev.swfHTML5 File:/content/generator/Course_90017383/sims_BNII_SBO_ProcessingNonbarcodedSamplesRev/index.htmlPDF File: Use the icons on the Info dialog's toolbar as shortcuts to six dialogs that you may access frequently. How do you use these icons? Roll your mouse anywhere in the toolbar to view the icon names. Click an icon to go directly to its corresponding dialog. (or you can take the long road by using the menus on the menu bar.) Now let's take a tour of the functions of each of these commonly used dialogs...   Welcome to the BN™ II System Software and Basic Operations course. In this introductory online course, you'll learn that the BN™ II applications software runs on an APPLE MACINTOSH computer and operating system, and you'll become familiar with icons on the desktop. You'll also learn to describe the layout of the Info dialog (the software's main dialog), to identify the functions of the software's six commonly used dialogs, and to describe how to perform basic operations, such as preparing the analyzer, processing samples, and performing maintenance. Note: The product names and/or brands referred to are the property of their respective trademark holders.