DCA Vantage® Analyzer Hemoglobin A1C Calibration and Quality Control Online Training

Welcome to the DCA Vantage® Analyzer Hemoglobin A1c Calibration and Quality Control Online Training course. The DCA Vantage® Analyzer is designed to automatically perform a number of internal optical, electronic and mechanical checks, including calibration verification, with every test.  In addition, with every new reagent lot, operators can scan the calibration card and the analyzer performs an automatic lot-specific calibration.  Quality Control testing assures the integrity of the reagents and detects errors that may result from improper testing technique. Note: This course includes information on Options and Functions in the system which may require security approval from your facility in order to access Note: Wear personal protective equipment, including safety glasses, and gloves. Use universal precautions when working with biohazardous materials. Select Next to continue.   Identify conditions under which HbA1c Quality Controls should be run Identify the steps to perform Calibration for Hemoglobin A1c Identify the steps to run HbA1c Quality Controls Recall HbA1c Calibration Data Recall Hemoglobin A1c Quality Control Data Upon successful completion of this course, you will be able to: Select Next to continue. Contrast media containing iodine are classified according to their chemical bond. Ionic contrast media dissociates into charged particles in blood and non-ionic contrast media dissociates into neutral particles in blood. Non-ionic contrast media are associated with substantially fewer allergic reactions but are more expensive. Congratulations.  You have completed the DCA Vantage® Analyzer Hemoglobin A1c Calibration and Quality Control Online Training Course.  Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz.  Note: These options must be enabled in the system. Security approval must be authorized in order to access these functions. Perform HbA1c Calibration The DCA Vantage® Analyzer automatically performs a number of internal optical, electronic and mechanical checks including calibration verification with every test. With every new reagent lot, the analyzer performs an automatic lot-specific calibration when the calibration card is scanned. Values for reagent calibration parameters are encoded on the calibration card provided with each box of the reagent cartridges. Scan the calibration barcode before using the reagent cartridges. Scanning the calibration card automatically records the information into the analyzer. The analyzer stores calibration information for up to 16 distinct reagent lots. If you do not scan the calibration curve into the system for the particular lot number of cartridges that are in use, the system software prompts you to scan the calibration card. You must scan the calibration card for a lot of reagent cartridges before you can use the lot on analyzer. Note: Wear personal protective equipment, including safety glasses, and gloves. Use universal precautions when working with biohazardous materials.   To scan the Hemoglobin A1c calibration card: Locate the dot on the analyzer next to the barcode track. Locate the barcode on the calibration card. Hold the card so that the barcode faces to the right. Insert the Calibration card into the top of the barcode track. Hold the Calibration card gently against the right side of the track and smoothly slide the card downward. A beep sounds to signal a successful scan. To return to the Home screen, select OK. Recall Calibration Data Use the Calibration Data screen to access calibration data including scan date and time, and lot number for DCA Vantage® Hemoglobin A1c test. To view calibration data: Select Recall from the Home screen. Select Calibration Data from the Recall menu. Select HbA1c to display HbA1c calibration data. Highlight the calibration data that you want to display then select View. Select Print to print the calibration data. Select Recall to return to the Recall menu. Run HbA1c Quality Controls All control results must be within the acceptable range before any patient sample is tested and the results are reported. If a control result is out of range, troubleshoot the system, correct any problems identified, and run the controls again. When control results are within range, you can test and report on patient samples. The DCA Vantage® system can display a reminder message when a QC test is due. You can set the QC test reminder message to display only or display and require a QC test for selected control tests each time a patient test is run. If you set the system to require a QC, you must run and pass the QC test before you can continue with patient tests. NOTE: Prepare the controls according to the manufacturer’s instructions. Good laboratory practice dictates that a quality control program be established in all laboratories. The program should consist of assaying control material, evaluation of control results and acceptable limits on controls. Reagent Cartridge storage and sample collection and handling practices are also incorporated into the quality control program. Follow these steps to run a control test: Ensure you are at the Home screen; otherwise the system does not recognize that a QC test is being run. The system will not save data to memory or prompt that a QC test due (if reminder is set). Locate the control card. One side of the control card is for a normal control and the other side is for an abnormal control. Lot specific Mean and Range Values for HbA1c are found on the control card in the control kit. Locate the dot on the system next to the barcode track. Locate the barcode on the Control card. Hold the card so that the barcode faces to the right. Insert the Control card into the top of the barcode track. Hold the Control card gently against the right side of the track and smoothly slide the card down. Scan the HbA1c Control Card. A beep sounds to signal a successful scan. If no beep sounds, repeat the scanning procedure. Scan the reagent cartridge and insert into the reagent cartridge compartment.   Quality Controls Conditions Run quality control under these conditions: At regular intervals determined by the laboratory procedures When using a new shipment of reagents When using a new lot number of reagent Each time a calibration card is scanned Whenever test results are in doubt When training new operators To run quality control: Prepare the Controls according to the manufacturer’s instructions. Remove and unwrap the capillary holder from the Reagent Kit. Mix the control sample well by inversion or using a tube mixer. Open the control bottle. Avoid introducing air bubbles into the sample. While applying pressure to the bulb of the dropper (found in the control kit), insert the tip of the dropper into the control solution. Release the pressure on the bulb to aspirate a small amount of control solution. Fill the glass capillary tube by touching it to the tip of the dropper and fill the tube. Touch only the tip of the capillary tube to the control material. If an air bubble is present in the filled tube, discard the capillary holder and fill a new one.  Prevent the control material from coming in contact with the plastic part of the capillary holder.  If control material comes in contact with the capillary holder, discard the capillary holder. Squeeze any excess control material out of the dropper into the control bottle. Close the control bottle. Wipe any control solution off the sides of the glass capillary tube using a lint-free tissue. Do not touch the tissue to the open end of the tube. Contact with the open end could result in a loss of the sample. Inspect the capillary holder for the presence of any bubbles. If bubbles are obvious, discard the capillary and repeat the collecting procedure. Place the capillary holder in the correct position to insert it into the reagent cartridge. Insert the capillary holder into the reagent cartridge until the holder gently snaps into place. Avoid harsh insertion of capillary holder. Do not dislodge the sample in the glass capillary because erroneous results may occur. Recall Quality Control Data   Use the Control Tests Data screen to access Quality Control data including test results, run date and time, lot number, and expiration date. The steps required to access the Control Tests Data screen is the same sequence as to recall Calibration Data. Select Next to continue.   Otra tarea de configuración del Control de Calidad implica el uso de la pantalla Gráfica CC para asignar ensayos a ficheros específicos y organizar así los resultados de CC en grupos. Hasta cinco ficheros están disponibles para ayudarle a organizar el Control de Calidad. Por ejemplo, puede asignar todos los parámetros de ensayo que analicen los controles normales a un fichero y los controles patológicos a otro. O se podrían asignar todos los ensayos especializados a un fichero específico. También puede personalizar el nombre de los ficheros de control de calidad. Al asignar los ensayos a los ficheros, antes de comenzar a trabajar con un nuevo lote de control , debe agregar manualmente el número de lote de control a cada gráfica para que el sistema sepa en qué gráfica de CC debe mostrar los resultados. The analyzer stores calibration information for up to 16 distinct reagent lots of DCA HbA1c reagent cartridges. Use the lot-specific calibration card provided in the kit to scan the calibration barcode information prior to scanning the first reagent cartridge barcode. Scanning the calibration card allows access to the appropriate calibration parameter values and calibration curve for the particular lot of reagent in use. The values for reagent calibration parameters are encoded on the calibration card provided with each kit box of reagent cartridges. Scanning the calibration card automatically records the information into the DCA® Vantage analyzer. The calibration card for a lot of reagent cartridges must be scanned before that lot can be used on the system. The calibration card contains the reagent lot number, mean values, value ranges, and expiration date in both barcode and printed format. Note: If you do not scan the calibration curve into the system when using a new lot of HbA1c reagents, the system software prompts you to scan the calibration card. It is necessary to scan only one calibration barcode per lot number.  Additional cartridges or boxes from the same lot number may be used without rescanning the calibration card information.   Scanning the Hemoglobin A1c Calibration Card Learn about scanning the Hemoglobin A1C calibration card. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90006290/1_sim_DCAV_ScanHbA1cCalCard_HTML5_800x600_9/1_sim_DCAV_ScanHbA1cCalCard_HTML5_800x600_9.swfHTML5 File:/content/generator/Course_90006290/1_sim_DCAV_ScanHbA1cCalCard_HTML5_800x600_9/index.htmlPDF File: Enhorabuena. Acaba de terminar el Curso de Formación online de Control de calidad del sistema Sysmex®/CS-2100 . Le recomendamos que revise la información de esta página antes de proceder a la evaluación del curso. Use the Calibration Data screen to access calibration data including scan date and time, and lot number for DCA Hemoglobin A1c. Recalling Calibration Data Learn how to view calibration data. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90006290/2_sim_DCAV_RecallHbA1cCalData_HTML5_800x600_9/2_sim_DCAV_RecallHbA1cCalData_HTML5_800x600_9.swfHTML5 File:/content/generator/Course_90006290/2_sim_DCAV_RecallHbA1cCalData_HTML5_800x600_9/index.htmlPDF File: When doing a biopsy using the lateral arm, a zero degree scout view is taken first to see if the area of interest is visualized in the 5 X 5 field of view.  The ideal patient positioning would place the lesion in the center of the window but is not always possible due to the location of the lesion within the breast.  If desired, after the scout image has been taken, the settings can be changed from AEC to manual mode resulting in uniform image quality throughout the remainder of the procedure. The stereo pair will be taken next, and you will be ready to mark your target. Good laboratory practice dictates that a quality control program be established in all laboratories. The program should consist of assaying control material, evaluation of control results and acceptable limits on controls. Reagent Cartridge storage and sample collection and handling practices also are incorporated into the quality control program. Refer to your laboratory quality assurance program to ensure quality throughout the entire testing process. Follow the manufacturer's storage and handling instructions for quality control material. Improper storage and handling of control materials can cause erroneous results. Refer to the control material package insert for proper handling instructions.   Run Quality Controls under the following conditions: Regular intervals determined by the laboratory procedures Using a new shipment of reagents Using a new lot number of reagent Scanning a calibration card Test results are in doubt Training new operators Caution: When working with biohazardous materials, always use universal precautions. Wear personal protective equipment, including safety glasses, and gloves.   Running Quality Control Learn more about running HbA1c Quality Controls on the DCA Vantage® Analyzer. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90006290/3_sim_DCAV_RunHbA1cQC_HTML5_800x600_9/3_sim_DCAV_RunHbA1cQC_HTML5_800x600_9.swfHTML5 File:/content/generator/Course_90006290/3_sim_DCAV_RunHbA1cQC_HTML5_800x600_9/index.htmlPDF File: Preparing Quality Control Solutions Learn how to prepare quality control solutions. Prepare the Controls according to the manufacturer’s instructions. Remove and unwrap the capillary holder from the Reagent Kit. Mix the control sample well by inversion or using a tube mixer. Open the control bottle. Avoid introducing air bubbles into the sample. While applying pressure to the bulb of the dropper (found in the control kit), insert the tip of the dropper into the control solution. Release the pressure on the bulb to aspirate a small amount of control solution. Fill the glass capillary tube by touching it to the tip of the dropper and fill the tube. Touch only the tip of the capillary tube to the control material. If an air bubble is present in the filled tube, discard the capillary holder and fill a new one.  Prevent the control material from coming in contact with the plastic part of the capillary holder.  If control material comes in contact with the capillary holder, discard the capillary holder. Squeeze any excess control material out of the dropper into the control bottle. Close the control bottle. Wipe any control solution off the sides of the glass capillary tube using a lint-free tissue. Do not touch the tissue to the open end of the tube. Contact with the open end could result in a loss of the sample. Inspect the capillary holder for the presence of any bubbles. If bubbles are obvious, discard the capillary and repeat the collecting procedure. Place the capillary holder in the correct position to insert it into the reagent cartridge. Insert the capillary holder into the reagent cartridge until the holder gently snaps into place. Avoid harsh insertion of capillary holder. Do not dislodge the sample in the glass capillary because erroneous results may occur.   When complete, select the X in the upper-right corner to close this window and continue. The DCA Vantage® Analyzer displays a reminder message when a QC test is due. The QC test reminder message can be set to display a QC reminder only or require a QC test for selected control tests.  If the system is set to require QC, you must run and pass the QC test before proceeding with patient tests. Control results must be within the acceptable range before any patient sample is tested and the results are reported. If a control result is out of range, troubleshoot the system, correct the problems then run the controls again. After the control results are within range, you can test and report on patient samples. Note: These options must be enabled in the system. Security approval must be authorized in order to access these functions. Note: Prepare the controls according to the manufacturer’s instructions. Select Next to continue.   En esta sección, usted conocerá las distintas áreas de la pantalla táctil, donde aparece información específica diversa y la identifican de los iconos en el área de estado del sistema . Use the Control Tests Data screen to access Quality Control data including test results, run date and time, lot number, and expiration date. The steps required to access the Control Tests Data screen is the same sequence as Recall Calibration Data (see Recall Calibration). Select Next to continue.