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Dimension® EXL™ 200 Integrated Chemistry System IMT Online Training

Replace consumables to maintain accurate results. Perform a condition and dilution check after changing the Multisensor. This clinical laboratory training qualifies for continuing education units (CEU).

Describe how the IMT pump alignment ensures accurate sample positioning across the QuikLYTE® multisensor Explain how to replace IMT consumables and run a condition and dilution check after a multisensor replacement Identify components of the IMT system and their functions Recognize IMT system calibration concepts Trace the flow of samples from the 1:10 dilution through the IMT system Welcome to the Integrated Multisensor Technology Assay Measurement online training for on the Dimension® EXL™ 200 Integrated Chemistry System. In this course, you will learn how to: Select Next to continue. IMT system features include: QuikLYTE® Multisensor which measures Na+, K+, and CI- Small sample volume requiring only 40 µL of serum, plasma or urine Indirect sample measurement using a 1:10 dilution Rapid turnaround time for results: 57.6 seconds No daily maintenance  Select Next to continue. Welcome to the CellaVision System Maintenance Online Training course.  The CellaVision systems require the operator to perform daily, weekly and remedial maintenance to maintain proper analyzer efficiency. Select Next to continue. In this section, you will learn how to identify the components of the IMT system and understand thier functions. IMT Components and Functions Learn more about each component. Slide NumberText BlocksCalloutsAudio ScriptImage File1 In this section, you will learn about IMT components and their functions. Select Next to continue.   Note: If audio does not automatically start, select the play arrow in the top left to begin. In this section, you will learn about IMT components and their functions.2 IMT Pump The IMT pump moves samples and fluids through the IMT system. Select Next to continue.  The IMT Pump moves samples and fluids through the IMT system.3 QuikLYTE® Multisensor The IMT tower holds the QuikLYTE® multisensor. The 2 main components within the cartridge housing are: Ceramic sensor substrate chip with Na+, K+, Cl-, and reference electrodes Elastomer flow channel for the standard or patient sample and salt bridge solution to pass through separately  Select Next to continue. Select each number to review the corresponding text. Callouts Ceramic Chip  IMT TowerThe IMT tower holds the QuikLYTE® Multisensor and includes two main components within the cartridge housing: Ceramic sensor substrate chip with sodium, potassium, chloride and reference electrodes elastomer flow channel for the sample and salt bridge solution to pass through separately4 Photometric Sample Probe The photometric sample probe: Aspirates and delivers samples to the IMT port. Level senses samples in tubes. Detects fluid in sample cups and small sample containers (SSCs). Select Next to continue. Select the number to review the corresponding text.CalloutsPhotometric Sample ProbeThe Photometric Sample Probe aspirates and delivers samples to the IMT port, level senses samples in tubes, and detects fluid in Sample Cups and Small Sample Containers.5 IMT Port The IMT port is the receptacle where the 1:10 sample dilution is mixed. Select Next to continue. Select the number to review the corresponding text.CalloutsIMT PortThe IMT Port is the receptacle where the 1:10 sample dilution is mixed.6 IMT Rotary Valve The IMT rotary valve controls air and fluid segments that enter the IMT system. Select Next to continue. Select the number to review the corresponding text.CalloutsIMT Rotary ValveThe IMT Rotary Valve controls air and fluid segments that enter the IMT system.7 IMT Diluent Pump The IMT diluent pump controls the amount of sample diluent delivered to the IMT port for the 1:10 sample dilution. Select Next to continue.    Select the number to review the corresponding text. CalloutsIMT Diluent PumpThe IMT Diluent Pump controls the amount of sample diluent delivered to the IMT port for the 1:10 sample dilution.8 QuikLYTE® Sample Diluent QuikLYTE® Sample Diluent is used to make the 1:10 sample dilution. Select Next to continue.    Select the number to review the corresponding text.CalloutsQuikLYTE® Sample DiluentQuikLYTE® Sample Diluent is used to make the 1:10 sample dilution.9Salt Bridge Solution Salt Bridge Solution is a reference solution that provides a constant electrical potential for electrolyte measurements. Select Next to continue.   Select the number to review the corresponding text. CalloutsSalt Bridge SolutionSalt Bridge Solution is a reference solution that provides a constant electrical potential for electrolyte measurements.10 IMT Fluids Standards A and B (prepared as 1:10 dilutions) are used for IMT system calibrations. No additional dilutions of the standards are made by the IMT system. Flush solution is used to clean and rinse the IMT port and to separate samples to prevent carryover. Select Next to continue.    Select each number to review the corresponding text.CalloutsFlushStandards A & BStandards A and B (prepared as 1:10 dilutions) are used for IMT system calibrations. No additional dilutions of the standards are made by the IMT system. Flush solution is used to clean and rinse the IMT port and to separate samples to prevent carryover.11 Flush Pump The flush pump delivers flush solution to the IMT port. Select Next to continue.    Select the number to review the corresponding text.CalloutsFlush PumpThe Flush Pump delivers flush solution to the IMT port.12 Congratulations. You have learned to identify the major components of the IMT system.    When complete, select the X in the upper-right corner to close the window and continue.Congratulations. You now are familiar with the components of the IMT system. There are two CellaVision Digital Microsopy systems, the CellaVision DM96 and the CellaVision DM1200.  The main hardware components of both systems consist of the Slide Scanning Unit (SSU) and the Computer System. Select the appropriate link below to learn more about the hardware components of your laboratory's CellaVision system. IMT components are connected by a series of tubings that carry fluid and air segments through the system. It is important to understand how the components are connected, as well as what happens in each tubing section, so that you can effectively: Monitor the IMT system during electrolyte processing. Perform IMT maintenance. Troubleshoot IMT problems. Tip:  This diagram is also found inside the left cabinet door of your instrument. Select Next to continue.   The processing of peripheral blood slides on the CellaVision system can be completed in a few simple steps.  The quality of the process is dependent on the quality of the peripheral smear.  A properly prepared smear must have clipped, rounded or beveled corners and meet the proper staining protocol. Select the links below to learn more about peripheral blood slide preparation and processing slides on each of the CellaVision systems. Download and print a copy of the IMT Flow Diagram and Compartment ID Chart.  This diagram can also be located in the Appendix of the Operator's Guide and inside the left cabinet door of the instrument.   IMT Fluidics Learn about IMT fluidics. Base ImageHotspotsText BlocksImage File IMT Pump  X tubing stretches around the IMT pump from the X2 tubing (black) to the X3 tubing (black). QuikLYTE® Multisensor IMT Tower which houses the QuikLYTE® multisensor: Patient and standard samples enter the sample channel through the X1 tubing (black). Salt Bridge Solution enters the reference channel through the R1 tubing (blue). Both samples and solution exit the sensor through the X2 tubing (black).  QuikLYTE® Sample Diluent  Sample diluent connects to the diluent pump through the D1 tubing (green) and enters the IMT port through the D2 tubing (green).  Salt Bridge Solution  Salt Bridge Solution is pumped to the reference channel of the multisensor through the R1 tubing (blue). The R1 tubing is seated inside the pinch valve on the back of the IMT tower.  IMT Port  In the IMT port: Diluent enters through D2 tubing (green). Flush enters through F2 tubing (yellow). Sample exits through X0 tubing (red). IMT Rotary Valve  The IMT Rotary Valve controls the entry of air and liquids into the IMT system:  Liquids and air enter the rotary valve through the A tubing (Standard A), B tubing (Standard B), and X0 tubing (diluted sample and Flush) (all red) and air inlet port Air-liquid segments exit the rotary valve through the X1 tubing (black)  Diluent Pump  At the IMT diluent pump, diluent enters the pump through D1 tubing (green) and exits through D2 tubing (green) to the IMT port.  Flush Pump   The flush pump connects to flush solution through the F1 tubing (yellow), and connects to the IMT port through the F2 tubing (yellow). Standard A  Standard A is delivered to the IMT rotary valve through the A tubing.  Standard B  Standard B is delivered to the IMT rotary valve through the B tubing.  Flush   Flush solution connects to the flush pump through the F1 tubing and enters the IMT port through the F2 tubing.  The platelet tab allows the user to count or estimate the platelet concentration as well as exclude the platelet analysis from the final results.  Select Next to continue.   The following key features of the fluid flow process are: The IMT system performs an indirect measurement. All samples are diluted 1:10 prior to being measured. Liquids flow through IMT system tubings in an alternating pattern of air and liquid segments. The IMT system pulls air and liquids through the system using the peristaltic action of the IMT pump. Select Next to continue. Weekly maintenance should be completed on the CellaVision system to ensure efficient performance. Select the appropriate link below to learn more about weekly maintenance on your laboratory's CellaVision system. The IMT system performs an indirect measurement on all samples, which means all patient and control samples are diluted 1:10 prior to being measured. IMT 1:10 Sample Dilution Process Learn how the IMT system performs the 1:10 sample dilution. Checklist TitleChecklist TypeChecklist ContentDiluent Delivered to IMT PortHTML The IMT diluent pump delivers 320 µL of QuikLYTE® sample diluent to the IMT port. Select each checkbox to learn more about tasks performed by the IMT system during the sample dilution process.Sample Delivered to IMT PortHTML The sample probe delivers the sample to the port and ultrasonically mixes it with the diluent.Sample Enters IMT SystemHTML The IMT pump pulls the diluted sample into the IMT system.Circuit Checks for Liquid PlacementHTML Air and liquid detectors check for proper sample placement in the QuikLYTE® multisensor. Select the X in the upper-right corner to close the window and continue. Liquids flow through IMT system tubings in an alternating pattern of air and liquid segments. Liquids pulled into the system include the following: 1:10 diluted sample for measurement Standards A or B for calibrations Flush solution to clean tubings and prevent carryover from one sample to the next The process begins as the IMT pump pulls liquid into the IMT rotary valve, which rotates to insert a segment of air from the air inlet port into the liquid stream, followed by flush solution from the IMT port. This action creates a pattern of air, sample, and flush liquid segments that move through the tubing for measurement by the QuikLYTE® multisensor. Note: The IMT system creates the same air/liquid pattern for all liquids that flow through the system: serum, plasma, or urine, as well as Standards A and B. Select Next to continue. Electrolytes are measured in the QuikLYTE® multisensor after samples are moved into the tower.  IMT Measurement Click START to view this process. IMT Measurement View the measurement process. Slide NumberText BlocksCalloutsAudio ScriptImage File1Let’s follow a sample as it enters the IMT system to learn how electrolytes are measured. Select Next to continue.Note: If audio does not automatically start, select the play arrow in the top left to begin.Let's follow a sample as it enters the IMT system to learn how electrolytes are measured.2Sample Flow The IMT pump draws the diluted sample from the IMT port through X0 tubing to the IMT rotary valve. From there, the diluted sample flows through the X1 tubing into the IMT tower. Select Next to continue.Select each number to review the corresponding text.CalloutsIMT PortX0 TubingIMT Rotary ValveX1 TubingThe IMT pump draws the diluted sample from the IMT port through X0 tubing to the IMT rotary valve. From there, the diluted sample flows through the X1 tubing into the IMT tower.3Positioning Sample In the IMT tower, the sample portion of the liquid segment is positioned across the QuikLYTE® multisensor. The multisensor takes continuous conductivity readings to verify sample positioning. Select Next to continue. In the IMT tower, the sample portion of the liquid segment is positioned across the QuikLYTE® Integrated Multisensor. The multisensor takes continuous conductivity readings to verify sample positioning. Later in this course, you will learn how the IMT pump performs an automatic pump alignment to ensure proper positioning of the sample.4IMT Pump The movement of the air and liquid through the system is controlled by the IMT pump. Sensors take readings exactly when the liquid segment (sample or standard) is positioned in the multisensor. Select Next to continue. The movement of the air and liquid through the system is timed by the IMT pump so that the sensors take readings exactly when the liquid segment which could be sample or standard is positioned in the multisensor.5Multisensor Readings Multisensor readings determine: When the previous sample has been removed by detecting that air is present. When a new sample is present by detecting that a liquid segment is present. The potential of each Na+, K+, and Cl- ion selective electrode is measured against the reference electrode to determine electrolyte levels in the sample. Select Next to continue. Multisensor readings determine when the previous sample has been removed by detecting that air is present, and when new sample is present by detecting that a liquid segment is present. The potential of each sodium, potassium and chloride ion selective electrode is measured against the reference electrode to determine electrolyte levels in the sample. Salt Bridge which flows through R1 tubing serves as the reference solution.6Exiting the IMT System After measurement, the Salt Bridge Solution and diluted sample exit the IMT tower through the X2 tubing and around the IMT pump through X tubing to the X3 waste tubing. Select Next to continue. Select each number to review the corresponding text.CalloutsX2 TubingX3 TubingAfter measurement, the Salt Bridge Solution and diluted sample exit the IMT tower through the X2 tubing and around the IMT pump through the X tubing to the X3 waste tubing.7Congratulations. You have learned how the IMT system takes electrolyte measurements.When complete, select the X in the upper-right corner to close the window and continue.Congratulations. Now that you have learned how the IMT system takes electrolyte measurement, let's look at monitoring consumables. The IMT system monitors when you need to replace IMT consumables. Sample Processing If an IMT consumable needs to be replaced and the Load List contains patient samples with orders for electrolyte tests, the yellow Needs Check icon in the Operating Conditions Status area of the screen is replaced by the red Check Needs icon. IMT Calibration If a calibration is due while an IMT consumable needs to be replaced, an "Insufficient IMT Consumables" error message appears in the Error Message area of the screen. Responding to System Needs Learn about responding to System Needs. Respond to System Needs To respond to System Needs: Select Alt+N to view the System Needs screen. Select the appropriate function key to view the System Need. When complete, select the X in the upper-right corner to close the window and continue.   Responding to IMT Consumable Error Messages Learn about responding to IMT consumable error messages. Respond to IMT Consumables Error Messages To respond to an Insufficient IMT Consumables error message: Select Alt+M. Use the Change IMT Consumables screen to determine which consumables need to be replaced. When complete select the X in the upper-right corner to close the window and continue.   Hardware errors on the CellaVision include hardware startup errors and processing errors. Hardware startup errors can be caused by loose or disconnected cables, jams, low oil levels or having the system hatch open.  Processing problems include similar causes as well as dirty air filters and critical temperatures.  All hardware errors, causes and required actions can be located in the CellaVision User's Guide. Select the links below to learn more about magazine and/or slide jams on your CellaVision system. The Change IMT Consumables screen displays the number of assays and/or time remaining before a consumable needs to be replaced. Press the appropriate function key to replace an IMT consumable, update counts, and store changes. When you replace IMT fluid consumables, the system automatically: Primes the solution that was replaced. Schedules an IMT calibration if you replace Standard A, Standard B, or Salt Bridge or Flush solution. Performs an IMT calibration after exiting the Change IMT Consumables screen. When you replace the sensor, the system automatically: Primes Salt Bridge solution. Displays the IMT Condition and Dilution Check screen. Replacing IMT Consumables Click START to take a closer look at replacing IMT consumables. Replacing IMT Consumables Learn about replacing IMT consumables. Slide NumberText BlocksCalloutsAudio ScriptImage File1To view the Change IMT Consumables screen, from the Operating Menu, select: F4: SYSTEM PREP F3: IMT F1: CHANGE CONSUM The Change IMT Consumables screen appears. Reminder: Press the appropriate function key to update the count and then F8: STORE CHANGES to store any changes. Select Next to continue.Note: If audio does not automatically start, select the play arrow in the top left to begin.In this section, you will learn how to replace IMT consumables. To view the Change IMT Consumables screen, select F4: System Prep, F3: IMT then F1: Change Consumables2Standards A and B and Flush Solution Standard A is used when the IMT system performs: A 2-point calibration. A 1-point calibration to check for drift after processing a sample. An automatic pump alignment. Standard B is used only to perform a 2-point calibration. Select Next to continue.Tips: Standard A and B are stable for 21 days on the instrument after opening. Standard A, Standard B, and Flush bags must be positioned in their tray with the fittings down. As the Standards and Flush are consumed, roll the bags to minimize condensation. Standard A is used when the IMT system performs a two-point calibration, a one-point calibration to check for drift after processing a sample or an automatic pump alignment. Standard B is used only to perform a two-point calibration. Here are some Tips: Standards A and B are stable for 21 days on the instrument after opening. Standard A, Standard B and Flush bags must be positioned in their tray with the fittings down. As the Standards and Flush are consumed, roll the bags to minimize condensation.3Salt Bridge Solution and Sample Diluent Note the following when replacing Salt Bridge Solution and Sample Diluent: Do not pool Salt Bridge Solution or QuikLYTE® Sample Diluent. Do not replace the Salt Bridge Solution or QuikLYTE® Sample Diluent at the same time. If the 2 dip tubes touch, you could contaminate the liquid in the new bottles. QuikLYTE® Sample Diluent is stable for 21 days on the instrument after opening. Select Next to continue.Select each number to review the corresponding text. CalloutsSample DiluentSalt Bridge SolutionHere are some Tips when changing the Salt Bridge Solution and Diluent: Do not pool Salt Bridge Solution or QuikLYTE Sample Diluent. If the two dip tubes touch, you could contaminate the liquid in the new bottles. QuikLYTE® Sample Diluent is stable for 21 days on the instrument after opening.4QuikLYTE® Multisensor Note the following when replacing the QuikLYTE® Multisensor: Replace the multisensor after 5 days or 1,000 samples, whichever occurs first. After you replace the sensor, the system primes the Salt Bridge Solution. When priming is complete, the IMT Condition and Dilution Check screen appears so that you can run a condition and dilution check. Select Next to continue.Now let's look at tips when changing the multisensor. Replace the multisensor after 5 days or 1000 samples, whichever occurs first. After you replace the sensor, the system primes the Salt Bridge Solution. When priming is complete, the IMT Condition and Dilution Check screen appears so that you can run a condition and dilution check. This will condition the new sensor and check the accuracy and precision of the 1:10 dilution. 5 Congratulations. You have learned how to replace IMT consumables . When complete, select the X in the upper-right corner to close the window and continue.Congratulations. Now that you have learned about replacing IMT consumables, let's look at how to perform a Condition and Dilution Check. The Condition and Dilution Check is performed to condition a new sensor and check the accuracy and precision of the 1:10 dilution. Tips: The QuikLYTE® Dilution Check solution must be refrigerated when not in use. Do not use when less than 1 inch of solution remains in the bottle. Do not pipette out of Dilution Check solution bottle. The dilution check follows conditioning, IMT auto alignment, and calibration. Running a Condition and Dilution Check Learn how to run a Condition and Dilution Check. Checklist TitleChecklist TypeChecklist ContentEnter Segment PositionHTML Type a segment position in the Start at Position field, and then press Enter. Select each checkbox to learn more about running a condition and dilution check from the IMT Condition and Dilution Check screen.Update Assigned PositionsHTML Press F4: COND & DILCHK to update the screen with assigned positions for the conditioning sample and dilution check solution.Load Sample CupsHTML Load 2 sample cups containing conditioning sample (fresh serum or heparinized plasma) and dilution check solution into assigned segment positions.Start the RunHTML Press F5: START to run the conditioning cycle, followed by a pump alignment, IMT calibration, and then the dilution check.Review ReportHTML Review the dilution check on the printout. Select the X in the upper-right corner to close the window and continue. Dilution Check: Pass Results are within acceptable ranges when: Na+ ≤ 1.4 sd K+ ≤ 0.04 sd % bias ≤ ± 1% If the ranges are acceptable, record the results on the Electrolyte Results Log sheet and then run the electrolyte QC. Note: SD is the precision check and % bias is the accuracy check. Dilution Check: Fail A dilution check can fail because: The bias is > + 1%. Standard deviation (sd) for Na+ is greater than 1.4, or for K+ is greater than 0.04. The dilution check did not completely process. % Bias Unacceptable If the bias is >1%, press CORRECT BIAS (F4>F3>F6>F1). At the prompt, Do you want the dilution calibration factor to be corrected? (y/n), type Y for yes. If the % bias corrects to 0%, record both the Failed and Passed dilution check results on the Electrolyte Results Log sheet and run electrolyte QC. If the % bias does not correct to 0%, the following message appears: The calibration factor is beyond the allowable limits. This message confirms that the % bias was unacceptable and was not close enough for the instrument to correct. Refer to the Operator’s Guide for instructions. Na+ and K+SD: Unacceptable Check the pump rate field on the Fluids Prime/Pump Alignment screen (F4>F3>F3). If the pump rate is less than 68 μL/revolution, replace the X tubing inside the IMT pump. Perform the Sample Probe to the IMT Port Alignment procedure in the Operator's Guide. Perform another dilution check using fresh Dilution Check Solution in a new sample cup. After repeating, call the Technical Solutions Center if the SDs are still unacceptable.   Note: If both bias and SD fail, always troubleshoot SD first. Process Errors Dilution check results will not print if the IMT auto alignment or calibration fails. Follow this procedure to resolve the failed dilution check: Troubleshoot the IMT error message. Rerun a dilution check. If the dilution check again does not process completely again, call the Technical Solutions Center. Select Next to continue. Performing an IMT pump alignment ensures accurate sample positioning across the QuikLYTE® multisensor. Positioning can be affected by:  IMT pump tubing wearing over time (replaced monthly). Protein buildup from serum and plasma. Pinched or leaking tubing. Impeded salt bridge flow. Select Next to continue. Before every calibration, the IMT system uses Standard A to perform an automatic pump alignment and then stores a pump rate on the Pump/Alignment screen. The nominal pump rate is in the range of 75-79 μL/revolution (with new X-tubing). The IMT pump pulls Standard A from the reagent bag and pumps small increments until the liquid is detected by the sensor as properly placed, resulting in the pump rate number. If the liquid segment is not detected during automatic pump alignment, an IMT FAILED TO AUTOALIGN error is displayed. Tip: To access the Fluids Prime / Pump Alignment screen, select: F4: SYSTEM PREP F3: IMT F3: ALIGN/PRIME Select Next to continue. The IMT system automatically performs a 2-point calibration every 2 hours to maintain precision and accuracy. If IMT calibration results meet system specifications, IMT OK appears in the Instrument Status area. If IMT calibration results do not meet system specifications, IMT NOT CALIB appears in the Instrument Status area. IMT Calibration Click START to learn about IMT Calibration. IMT Calibration Learn about IMT calibration. Slide NumberText BlocksCalloutsAudio ScriptImage File1 In this section, you will learn more about IMT calibration. Select Next to continue. Note: If audio does not automatically start, select the play arrow in the top left to begin.In this section, you will learn about IMT calibration.2IMT Calibration Schedule The IMT system calibrates: Automatically every 2 hours using Standard A and Standard B to perform a 2-point calibration. During a condition and dilution check. After changing certain IMT consumables. After performing an IMT automatic pump alignment. After a manual calibration request. Select Next to continue. When does the IMT system calibrate? Automatically every two hours using Standard A and Standard B to perform a two-point calibration. During a condition cycle and dilution check. After changing certain IMT consumables After performing an IMT automatic pump alignment And after a manual calibration request3What’s in the IMT System Status box? The IMT System Status box indicates the processing status of the IMT system. IMT OK (Green Box): The IMT system is turned on, calibrated, and ready to process IMT tests. Select Next to continue. The IMT System Status box in the Instrument Status area of your screen indicates the processing status of the IMT system. The following indicator may appear: IMT OK (green box) This indicates that the IMT system is turned on, calibrated, and ready to process IMT tests.4IMT System Status Box IMT Paused (Blue Box): All IMT system activity has been paused and the IMT system is waiting for a command. No action will occur until the operator presses a function key on the screen. Select Next to continue. IMT Paused (blue box) indicates that all IMT system activity has been paused and the IMT system is waiting for a command. No action will occur until the operator presses a function key on the screen.5IMT Status Box IMT Calibrating (Yellow Box): The IMT system is running a calibration. Select Next to continue.IMT Calibrating (yellow box) indicates the IMT system is running a calibration.6IMT System Status Box Na+, K+, or Cl- (Small Red Box): 1 or more sensors in the QuikLYTE® multisensor cartridge failed to calibrate. Select Next to continue. If you see Sodium, Potassium or Chloride in a small red box, this means one or more sensors in the QuikLYTE multisensor cartridge failed to calibrate.7IMT System Status Box Na+, K+, or Cl- (Small Yellow Box): Test has been overridden on the IMT Calibration screen. Select Next to continue. If you see Sodium, Potassium or Chloride in a small yellow box, this indicates that the test has been overridden on the iMT Calibration Screen.8IMT System Status Box IMT Not Calib (Red Box): The IMT system is not calibrated. You cannot process IMT tests. Select Next to continue. If IMT Not Calibrated appears in a red box, you cannot process IMT tests because the system is not calibrated.9IMT System Status Box IMT Not Config (Yellow Box): The IMT system has not been configured. You cannot process IMT tests. Select Next to continue.You also cannot process IMT tests if you see IMT Not Configured in a yellow box. This indicates that the IMT system has not been configured.10IMT Calibration Results Electrolyte slope specifications must be within ranges. These ranges never change: Na+ and K+ = 53 to 65 Cl- = -55 to -40 The air detect value must be greater than or equal to 0.8 and at least 2 times the liquid detect value The liquid detect value must be less than or equal to 0.6 Select Next to continue. Reminder: Record calibration results on the Electrolyte Maintenance Log sheets.Tip: From the IMT Calibration screen, press Help to view calibration specifications for the IMT system.You can review calibration result statistics on the IMT Calibration screen and the IMT Calibration Report printout. IMT calibration results must meet the following system specifications for a successful calibration. Electrolyte slope specifications must be within range. Sodium and potassium = 53 to 65 Chloride = -55 to -40 The air detect value must be greater than or equal to 0.8, and at least two times the liquid detect value. And the liquid detect value must be less than or equal to 0.6.11When Calibration Fails If IMT NOT CALIB appears in the IMT System Status box following an IMT calibration, the following occurs: The IMT calibration report printout will not display the slope. Asterisks appear in the slope field for the uncalibrated method. The IMT Calibration screen displays the slope reading with asterisks to the left of the reading. Select Next to continue.If “IMT NOT CALIBRATED” appears in the IMT System Status box following an IMT calibration, the following occurs: •The IMT calibration report printout will not display the slope, but will have asterisks in the slope field for the uncalibrated method. •The IMT Calibration screen will display the slope reading with asterisks to the left of the reading. 12Congratulations. You now have learned about IMT calibrations.When complete, select the X in the upper-right corner to close the window and continue.Congratulations. You now have learned about IMT calibrations. Congratulations. You have completed the IMT Assay Measurement training course for the Dimension® EXL™ 200 Integrated Chemistry System. Listed below are the key points that have been presented. Take time to review the material before you proceed to the final quiz. IMT Change Consumables screen Use the Change IMT Consumables screen to view IMT assay counts, replace consumables, update counts, and store changes.After changing the QuikLYTE® multisensor, perform an IMT condition and dilution check. The condition hydrates and pretreats a sensor and must be performed prior to running the dilution check. IMT Condition and Dilution Check Screen The dilution check monitors the accuracy and precision of the 1:10 sample dilution. Use the IMT Condition and Dilution Check screen to perform a dilution check by running 5 replicates of Dilution Check solution. The system compares current dilution check results to expected bottle values, and calculates a % bias. Dilution check results must meet standard deviation (sd) and % bias specifications. Results appear on the IMT Dilution Calibration screen as well as the printout. Resolve a Failed Dilution Check First, try to correct the % bias. If successful, run QC. If unsuccessful, replace the bottle of diluent and use a new bottle of Dilution Check Solution to rerun the dilution check. Pump Alignment IMT pump alignments ensure accurate sample positioning across the IMT multisensor. During a pump alignment, the peristaltic pump pulls Standard A in small increments until the sensor detects that the fluid is properly placed. The IMT system then records a pump rate number. A pump alignment is performed before every 2-point calibration to compensate for any variation in system tubing volumes and pumping efficiency. Review Calibration Results Use the IMT Calibration screen and printout to review calibration results. Successful IMT calibrations must meet system specifications for Na+ and K+ slope (53 to 65), Cl- slope (-55 to -40), air detect value (≥0.8 and at least two times the liquid detect value), and liquid detect value (≤0.6). From the IMT Calibration screen, select Help to view calibration specifications for the IMT system. If the calibration passes, the IMT System Status box displays IMT OK. If the calibration fails, the IMT System Status box displays IMT NOT CALIB or the Na+, K+, or Cl- test that failed to calibrate. Flow problems, the most frequent cause of a failed IMT calibration, are indicated by air/liquid detect values that do not meet system specifications. Select Alt+M for help in resolving IMT error messages. Select Next to continue.

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