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Dimension® EXL™ with LM Integrated Chemistry System HIL Feature Online Training

Configure system and reports to meet specific laboratory needs and day-to-day changes in workload and operation.

Demonstrate how to run HIL manually or automatically Describe how the HIL test is performed Identify how the HIL feature can alert you to potential test result interference from hemolysis, icterus, and/or lipemia in serum or plasma samples Interpret a test report with HIL information Program the HIL Operating Mode and enter Index Values Welcome to the HIL Feature course for the Dimension® EXL™ with LM integrated chemistry system. After completing this course, you will be able to: Select Next to continue. Congratulations. You have completed the HIL Feature course for the Dimension® EXL™ with LM integrated chemistry system. Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz. Describe how the HIL test is performed 20 µL of patient sample is pipetted into an empty cuvette along with system water.  Spectral absorbance measurements, taken at 405, 452, and 700 nm, are used to generate a sample specific HIL index. The HIL index is reported as a three-digit number. Each digit corresponds to an approximate concentration of a potential interferent:1st digit = H index (hemolysis) 2nd digit = I index (icterus) 3rd digit = L index (lipemia)   Describe how the HIL feature can alert you to potential test result interference from hemolysis, icterus, and/or lipemia in serum or plasma samples The HIL can be programmed to alert you with a test report message (HIL Interf) if an index value is greater than or equal to your specified HIL alert value. Program the HIL Operating Mode and enter Index values With the instrument in Standby, from the Operating Menu, press F5: Process Control > F8: More Options >F6: HIL Setup to display the password-protected HIL Setup screen. F1: Next Mode is pressed to select the Operating Mode, which can be OFF, ON, AUTO-ON-Tubes, Cups, SSCs or AUTO-ON-Tubes, Cups.  For either of the AUTO-ON options, Alert Index values between 2 and 6 must be entered for test methods.  Each of these index values corresponds to an approximate concentration range in mg/dL for each of the potential interferents. Demonstrate how to run HIL manually or automatically. Use the Enter Sample Data screen to request HIL manually for any serum or plasma sample by selecting Alt plus the HIL test key.  The HIL test also can be downloaded from the LIS or run automatically if the HIL operating mode is programmed.  Certain conditions must be met to run HIL automatically without operator intervention. Interpret a test report with HIL information. The HIL index will be reported first on the test report. The result is a 3 digit number and the Units are Ind.  Evaluate the HIL index to determine if you can report the results. Test reports also may display the HIL interf test report message if the Operating Mode has been setup for either AUTO-ON option.  If set to OFF or ON, HIL interf will not be displayed. Follow your laboratory’s procedures for reporting results when the sample is hemolyzed, icteric, and/or lipemic. Select Next to continue.     The HIL feature, based on the spectral characteristics of a serum or plasma sample, reports an index that can alert you to potential interference from hemolysis, icterus, and/or lipemia in the sample.  This provides a standard process for the assessment of the integrity of the sample instead of relying on visual observation. The HIL feature can be programmed to run automatically or by manual request on individual samples. Absorbance Measurements Learn about Absorbance Measurements To perform an HIL test, the system pipettes 20 µL of sample along with system water into an empty cuvette and derives the HIL index from the absorbance measurements. Spectral absorbance measurements are taken at: 405 nm for hemoglobin 452 nm for bilirubin 700 nm for turbidity If media does not automatically start, select the play arrow to begin. When complete, select the X  in the upper-right corner to close the window and continue.   HIL Test Reports Learn about HIL test reports. The index is reported as a three-digit number with the unit “Ind” where:   1st digit = H index (hemoglobin resulting from lysis of red blood cells) 2nd digit = I index (icterus resulting from endogenous bilirubin) 3rd digit = L index (lipemia or turbidity caused by insoluble lipids) Each digit corresponds to an approximate concentration for each of the potential interferents. The Dimension® EXL™ with LM integrated chemistry system can be programmed to alert you with the test report message "HIL Interf" when an index value is greater than or equal to your specified HIL alert value. When complete select the X in the upper-right corner to close the window and continue.   TEST RESULT REF INTERVAL UNITS HIL 114   Ind Method A 253 HIL Interf mg/dL Method B 15 7-18 mg/dL Method C 1.0 0.6-1.3 mg/dL Use the Enter Sample Data screen to manually request HIL for any serum or plasma sample. To request HIL manually: Press the Alt/HIL test key combination HIL is added to the list of requested test methods for the sample on the Enter Data screen You also can include HIL in a list of test requests downloaded from an LIS. HIL can run automatically without any operator intervention by programming the Dimension® EXL™ with LM integrated chemistry system or by download of the test order from the LIS. The following general conditions are required for HIL to run automatically: Sample must be serum or plasma only Sample must NOT be diluted Sample Mode must be primary tube, bar code tube, sample cup, or SSC (no limited cup-no level sense) HIL Operating Mode must be programmed as ON or AUTO-ON. In the AUTO-ON Mode, Alert Index values between 2 and 6 must be entered in the HIL Setup screen. NOTE: The instrument must be in Standby to program Index Values. HIL Setup Learn how to setup HIL. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90002884/DimensionSystem_HILSetup_HTML5_800x600_9/DimensionSystem_HILSetup_HTML5_800x600_9.swfHTML5 File:/content/generator/Course_90002884/DimensionSystem_HILSetup_HTML5_800x600_9/index.htmlPDF File: