Dimension Vista® System Calibration Online Training

Describe the theory of calibration and when it is necessary to perform calibration Enable automatic calibration features Input information from a calibrator Instructions for Use (IFU) sheet and load calibrator vials in vial carriers Set up and perform automatic calibrations Set up and perform manual calibrations using Routine and Advanced Functions screens Troubleshoot calibration problems After completing this course, you will be able to: Select Next to continue. Congratulations. You’ve completed the Dimension Vista® System Calibration Online Education Support Tool. Here’s a brief review: Calibration is the process of testing and adjusting a test system to provide a known relationship between the response measurement and the value of a substance measured by the procedure. To verify that the calibration of the test system remains stable, materials with known values are assayed in the same manner as patient samples. Calibration on the Dimension Vista® System takes place automatically, limiting the need for operator intervention. Automated calibration can be scheduled to take place within a time period convenient for your laboratory workflow. At a specified time before the calibrations are to begin, the system alerts you to upcoming calibrations and informs you of any calibration needs such as calibrators, QC, or reagent cartridges to be loaded. When to Calibrate A new calibration is required: For each new lot of Flex® reagent cartridges After major maintenance or service, or if indicated by quality control results As indicated in laboratory quality control procedures When required by government regulation When a method is assigned to a reagent server Calibration Theory Calibration processing follows these basic steps: Calibrators with known standard values produce a reaction that generates a signal response. Signal responses are converted into analyte units creating calibration curve coefficients. Adjustment coefficients are applied. The calibration curve and adjustment coefficients are stored. Results are sent to printer if this option has been set up on your system. Calibrators are test materials with defined values that establish the relationship between the response measurement and the analyte units. See the specific method’s Instructions for Use (IFU) for typical calibration levels. Calibration requires that the appropriate reagent cartridges and calibrators be loaded on the instrument. You must also scan the calibrator information (bottle values, etc.) from the barcode on the appropriate calibrator IFU. Calibrators are barcoded. Calibrator levels are color-coded. Most calibrators are liquid - 95%. Calibrators are loaded in vial carriers. Loading Calibrators Almost all of the calibrators are ready-to-use in a barcoded vial. A few require preparation before use. For those calibrators, you must transfer the prepared calibrator to a sample cup. Always refer to the preparation section of the calibrator IFU. Frozen calibrators must thaw at room temperature (22ºC - 28ºC). You can load calibrator vials while the system is processing samples. The vial barcode labels are scanned and the information is stored on the system. Place the required calibrator vials in a vial carrier. Make sure the barcode labels are entirely visible through the slots. Place the carrier in the loading area. Position the carrier with the labels facing away from you. Press the Load button. Dimension Vista® Calibrator Instructions for Use Each new lot of calibrators comes with an Instructions for Use (IFU) sheet. The calibrator IFU provides: Calibrator bottle values 2-D barcode The calibrator barcode contains the following information: Calibrator catalog number Calibrator lot number Fill volume Expiration date Configuring Automatic Calibration Features Calibration on the Dimension Vista® System takes place automatically, limiting the need for operator intervention. Automated calibration can be scheduled to take place within a time period convenient for your laboratory workflow. The Automatic Calibration features are programmed by method as follows: Access the Advanced>Configuration>Method Configuration screen. The Triggers tab is already enabled to initiate or trigger Automatic Calibration processing as needed. The QC tab is enabled when you are ready to verify calibration with QC. The Acceptance tab is already enabled to initiate Automatic Calibration Acceptance. Acceptance criteria are pre-programmed. Routine Functions Manual Calibrations Use the Routine Functions Manual Calibration feature to initiate calibration of a method when the automatic trigger calibration feature is not enabled. When a manual calibration is performed and acceptance of the results is not automatic, the operator must review and accept the calibration. Advanced Functions Calibrations The Advanced Functions Calibrations menu offers the following calibration options: Calibrators – Use this Advanced function to input the calibrator IFU information for a new lot of calibrators. Note: The use of this function is described in Automatic Calibration. Calibrations by Lot - Use this Advanced function to initiate manual calibration of a method. Note: You can also order calibration using the Routine functions calibration screen. The use of this function is described in Routine Functions Manual Calibration. Troubleshooting Occasionally, it may be necessary to troubleshoot a failed calibration. Before accepting the calibration, review all criteria listed in the Show Details screen. All acceptance criteria and instrument errors during processing and flags and comments found during calibration are displayed in the Acceptance Criteria Details part of the screen. If acceptance criteria fail, for example slope, intercept, or percent deviation, rerun calibration with new calibrators. If flags and comments appear during calibration, review the calibration errors for that specific method by pressing Advanced>Results>Test Status. Select Show Calibration Tests in the Data Filter box. Locate the calibration and review any flags and comments listed under the Error field to the right. Reject calibration and rerun calibration with new calibrators. If instrument errors occurred during calibration, troubleshoot the process errors alert (System>Process Errors>Active) and rerun calibration. If you need assistance troubleshooting calibrating data, contact the Technical Solutions Center (TSC) at 1-800-441-9250. Select Next to continue.   Calibration is the process of testing and adjusting a test system to provide a known relationship between the response measurement and the value of a substance measured by the procedure. To verify that the calibration of the test system remains stable, materials with known values are assayed in the same manner as patient samples.Calibration on the Dimension Vista® System takes place automatically, limiting the need for operator intervention. Automated calibration can be scheduled to take place within a time period convenient for your laboratory workflow. At a specified time before the calibrations are to begin, the system alerts you to upcoming calibrations and informs you of any calibration needs such as calibrators, QC, or reagent cartridges to be loaded. When to Calibrate A new calibration is required: For each new lot of Flex® reagent cartridges After major maintenance or service, or if indicated by quality control results As indicated in laboratory quality control procedures When required by government regulation When a method is assigned to a reagent server Calibration Theory Calibration processing follows these basic steps: Calibrators with known standard values produce a reaction that generates a signal response. Signal responses are converted into analyte units creating calibration curve coefficients. Adjustment coefficients are applied. The calibration curve and adjustment coefficients are stored. Results are sent to a printer if this option has been set up on your system. Select Next to continue. Calibrator Facts Calibrators are test materials with defined values that establish the relationship between the response measurement and the analyte units. See the specific method's Instructions for Use (IFU) for typical calibration levels. Calibration requires that the appropriate reagent cartridges and calibrators be loaded on the instrument. You must also scan the calibrator information (bottle values, etc.) from the barcode on the appropriate calibrator IFU. Calibrators are bar-coded. Calibrator levels are color-coded. Most calibrators are liquid - 95%. Calibrators are loaded in vial carriers. Loading Calibrators Almost all of the calibrators are ready-to-use in a bar-coded vial. A few require preparation before use. For those calibrators, you must transfer the prepared calibrator to a sample cup. Always refer to the preparation section of the calibrator IFU. Frozen calibrators must thaw at room temperature (22ºC - 28ºC). You can load calibrator vials while the system is processing samples. The vial barcode labels are scanned and the information is stored on the system.   Place the required calibrator vials in a vial carrier. Make sure the barcode labels are entirely visible through the slots. Place the carrier in the loading area. Position the carrier with the labels facing away from you. Press the Load button. Select Next to continue.   Each new lot of calibrators comes with an Instructions for Use (IFU) sheet. The calibrator IFU provides: Calibrator values 2-D barcode The calibrator barcode contains the following information: Calibrator catalog number Calibrator lot number Fill volume Expiration date Select Next to continue. Configuring Automatic Calibration Features Calibration on the Dimension Vista® System takes place automatically, limiting the need for operator intervention. Automated calibration can be scheduled to take place within a time period convenient for your laboratory workflow. Automatic calibration features are programmed by method. Automatic Calibration Procedure Access the Advanced>Configuration>Method Configuration screen. The Triggers tab is already enabled to initiate or trigger automatic calibration processing as needed. The QC tab is enabled when you are ready to verify calibration with QC. The Acceptance tab is already enabled to initiate automatic acceptance of the calibration. Acceptance criteria are pre-programmed.   Calibration Guidelines     Automatic Calibration Acceptance Criteria Learn more about the automatic calibration acceptance criteria. The following calibration acceptance criteria can be customized. Calibration reports can be generated and calibration data are stored onboard the system. Note: Contact the Technical Solutions Center (TSC) or your Technical Application Specialist (TAS) before modifying acceptance criteria. Percent Bias: recovered calibrator bottle values are within allowed recovered average percent bias for all levels (2-5). Bias is the difference between the recovered value and the bottle value multiplied by 100. Slope Range: slope of recovered bottle values versus actual is within allowed slope range. Allowable slope is defaulted for each method. Intercept Range: y-intercept of recovered bottle values versus actual is within allowed intercept range. The customer will assess if the intercept is not clinically significant. Correlation Range: correlation coefficient is within allowed correlation coefficient range. Typically 0.95-1.00, the allowable correlation range is defaulted for each method. Percent Deviation: deviation is the bias between the median of each level and the bottle value. The percent deviation is the absolute value of the percent bias (deviation) between the median and the bottle value for all levels including level 0 unless its bottle value has a concentration of less than 1.0-10. Percent Deviation Except Low: like percent deviation, but does not include level low. No QC flags when QC is run in association with calibration. There are additional criteria that can be enabled. These are listed at the bottom of the screen and are level specific: Recovery: average of recovered bottle value is within the allowable recovery range (accuracy) at each level. Percent Bias: recovered calibrator bottle values lie within the allowable recovered percent bias at each level (accuracy). SD: recovered calibrator bottle values are within the allowable recovered SD at each level (precision). Percent CV: recovered calibrator bottle values are within the allowable recovered percent cv at each level (precision). Delta: delta instrument response between paired calibrator levels is within the delta instrument response for each level pair, 1-2, 2-3, 3-4, 4-5. When complete, select the X in the upper-right corner to close the window and continue. Method Configuration Screen Learn about the Method Configuration Screen. Base ImageHotspotsText BlocksImage File Method Configuration QC Method Configuration Triggers Method Configuration Acceptance Automatic Calibration Procedure Learn how to input calibration data and initiate an automatic calibration. Checklist TitleChecklist TypeChecklist ContentInput Calibrator InformationSimulationInstructions:If media does not automatically start, select the play arrow to begin.  Select each checkbox to view the steps necessary to input information from the calibrator IFU and initiate an automatic calibration.Flash File:/content/generator/Course_90003140/Vista_AutoCaliAcceptCriteria_HTML5_Input_600x480_9/Vista_AutoCaliAcceptCriteria_HTML5_Input_600x480_9.swfHTML5 File:/content/generator/Course_90003140/Vista_AutoCaliAcceptCriteria_HTML5_Input_600x480_9/index.htmlPDF File:Load CalibratorsHTML   Load Calibrators 1. Place the required calibrator vials in a vial carrier, making sure the barcode labels are entirely visible through the slots. 2. Place the carrier in the loading area. Position the carrier with the labels facing away from you. 3. Press the Load button. Auto AcceptanceSimulationInstructions:If media does not automatically start, select the play arrow to begin.   When complete, select the X in the upper-right corner to close the window and continue. Flash File:/content/generator/Course_90003140/Vista_AutoCalAcceptance_HTML5_600x480_AutoAccept_9/Vista_AutoCalAcceptance_HTML5_600x480_AutoAccept_9.swfHTML5 File:/content/generator/Course_90003140/Vista_AutoCalAcceptance_HTML5_600x480_AutoAccept_9/index.htmlPDF File: Use the Routine Functions Manual Calibration feature to initiate calibration of a method when the automatic trigger calibration feature is not enabled. When a manual calibration is performed, acceptance of the results is not automatic; the operator must review and accept the calibration. Routine Functions Manual Calibration Process Learn more about the Routine Functions Manual Calibration process. The following table summarizes the steps required to perform a Routine Functions Manual Calibration. STEP SYSTEM NAVIGATION 1. Load calibrators. Check inventory. Setup > Inventory > Calibrator Vials 2. Load reagents. Check inventory. Setup > Inventory > Reagents 3. Order calibration. System > Method Summary > Select Method > Calibration > Order Calibration 4. Review, accept, and print reports. System > Method Summary > Select Method > Calibration > Accept/Reject Calibration Download and print the Routine Functions Manual Calibration Process job aid. When complete, select the X in the upper-right corner to close the window and continue. Routine Function Manual Calibration Learn how to perform a Routine Functions Manual Calibration. Checklist TitleChecklist TypeChecklist ContentInitiate Manual CalibrationSimulationInstructions:Note: If audio does not automatically start, select the play arrow in the top left to begin. Select each checkbox to learn how to perform a routine manual calibration.Flash File:/content/generator/Course_90003140/Vista_RoutineManualCalibration_HTML5_Initiate_600x480_9/Vista_RoutineManualCalibration_HTML5_Initiate_600x480_9.swfHTML5 File:/content/generator/Course_90003140/Vista_RoutineManualCalibration_HTML5_Initiate_600x480_9/index.htmlPDF File:Scan Rack BarcodeHTML   If the media does not automatically start, select the play arrow to begin. Press OKHTML Next, press OK and load the rack onto the instrument. The status field on the Calibration screen changes sequentially to Awaiting, Scheduling, Preparing Calibrators and Processing. Load Calibrator RackHTML   If the media does not automatically start, select the play arrow to begin. Accept CalibrationSimulationInstructions:If media does not automatically start, select the play arrow to begin. When complete, select the X in the upper-right corner to close the window and continue.Flash File:/content/generator/Course_90003140/VistaRoutineManualCalibration_HTML5_Accept600x480_9/VistaRoutineManualCalibration_HTML5_Accept600x480_9.swfHTML5 File:/content/generator/Course_90003140/VistaRoutineManualCalibration_HTML5_Accept600x480_9/index.htmlPDF File: The Advanced Functions Manual Calibrations menu offers the following calibration options: Calibrators – Use this Advanced function to input the calibrator IFU information for a new lot of calibrators.Note: Use of this function is described in Automatic Calibration. Calibrations by Lot - Use this Advanced function to initiate manual calibration of a method.Note: You can also order calibration using the Routine Functions Calibration screen. The use of this function is described in Routine Functions Manual Calibration. Advanced Functions Manual Calibration Process Learn about the Advanced Functions Manual Calibration process. The following table summarizes the steps required to perform Advanced Functions Manual Calibrations. STEP SYSTEM NAVIGATION 1. Scan calibrator IFU. Verify data. Advanced>Calibration>Calibrators 2. Load calibrator vials. Check inventory. Advanced>Inventories>Vial Inventory 3. Load Reagents. Check inventory. Advanced >Inventories>Reagent Inventory 4. Order calibration. Advanced>Calibration>Calibrations by Lot>Select Method>Order Calibration 5. Review, accept, and print reports. Advanced>Calibration>Calibrations by Lot>Select Method>Show Details>Accept/Reject Calibration   Download and print the Advanced Functions Manual Calibrations job aid. When complete, select the X in the upper-right corner to close the window and continue. Advanced Functional Manual Calibration Learn about Advanced Function Manual Calibrations. Checklist TitleChecklist TypeChecklist ContentInitiate Manual CalibrationSimulationInstructions:If media does not automatically start, select the play arrow to begin. Select each checkbox to learn more about initiating a manual calibration.Flash File:/content/generator/Course_90003140/VistaAdvancedManCalibrationInitiate_HTML5_600x480_9/VistaAdvancedManCalibrationInitiate_HTML5_600x480_9.swfHTML5 File:/content/generator/Course_90003140/VistaAdvancedManCalibrationInitiate_HTML5_600x480_9/index.htmlPDF File:Scan Rack BarcodeHTML   If the media does not automatically start, select the play arrow to begin. Select OKHTML Next, press OK and load the rack onto the instrument. The status field in the Calibration screen changes sequentially to Awaiting, Scheduling, Preparing Calibrators, and Processing. Load Calibrator RackHTML   If the media does not automatically start, select the play arrow to begin. Accept CalibrationSimulationInstructions:If media does not automatically start, select the play arrow to begin. When complete, select the X in the upper-right corner to close the window and continue.Flash File:/content/generator/Course_90003140/Vista_ADVManualCalibrationAcceptCal_HTML5_600x480_9/Vista_ADVManualCalibrationAcceptCal_HTML5_600x480_9.swfHTML5 File:/content/generator/Course_90003140/Vista_ADVManualCalibrationAcceptCal_HTML5_600x480_9/index.htmlPDF File: Occasionally, it may be necessary to troubleshoot a failed calibration. Before accepting the calibration, review all criteria listed in the Show Details screen. All acceptance criteria, flags and comments found during calibration, and instrument errors during processing are displayed in the Acceptance Criteria Details part of the screen. If acceptance criteria fail, for example slope, intercept, or percent deviation, rerun calibration with new calibrators. If flags and comments appear during calibration, review the calibration errors for that specific method by pressing Advanced > Results >Test Status. Select Show Calibration Tests in the Data Filter box. Locate the calibration and review any flags and comments listed under the Error field to the right. Reject calibration and rerun calibration with new calibrators. If instrument errors occurred during calibration, troubleshoot process errors alert (System > Process Errors > Active) and rerun calibration. If you need assistance troubleshooting calibrating data, contact the Technical Solutions Center (TSC). Calibration Troubleshooting Learn about troubleshooting Dimension Vista® System calibration problems. Slide NumberText BlocksCalloutsAudio ScriptImage File1Welcome back to the Virtual Lab. In this segment, we will learn how to troubleshoot calibration problems. Let’s get started. From the Home screen displayed here, click the Advanced icon to display the Advanced functions menu.Note: If audio does not automatically start, select the play arrow in the top left to begin.In this segment, we will learn how to troubleshoot calibration problems. Let’s get started! From the Home screen displayed here, click the Advanced icon to display the Advanced functions menu. 2Select Calibrations by Lot option 1. From the Advanced function menu, click the Calibration icon.  Next, from the Advanced functions menu, click the Calibration icon. Click the drop-down arrow to display Calibration options. Then, click the Calibrations by Lot option. Go ahead and perform these steps now. 3Select Calibrations by Lot option 1. From the Advanced function menu, click the Calibration icon. 2. Click the drop-down arrow to display the menu of Calibration options.  Enter your script here.4Select Calibrations by Lot option 1. From the Advanced function menu, click the Calibration icon. 2. Click the drop-down arrow to display the menu of Calibration options. 3. Click the Calibrations by Lot option.  Enter your script here.5Calibrations by Lot screen1. On the Calibrations by Lot screen that appears, select the appropriate method to be reviewed.Note: For Dimension Vista® 3000T Systems, use the twin selector to designate left or right instrument.2. Click the Show Details button from the Actions menu to access the Calibration Details screen. Go ahead and do this now.On the Calibrations by Lot screen that appears, select the appropriate method to be reviewed, as shown here. Then, click Show Details from the Actions menu to access the Calibration Details screen. Go ahead and do this now. 6Troubleshooting an Overall Acceptance Criteria Failure Review the Acceptance Criteria Details to determine which criteria failed.   If configured criteria fail, for example slope, intercept, or percent deviation, rerun calibration with new calibrators. If flags and comments occur during calibration, review the calibration errors for that specific method by pressing Advanced>Results>Test Status. Select Show Calibration Tests in the Data Filter box. Locate the calibration and review any flags and comments listed under the Error field to the right. Reject calibration and rerun calibration with new calibrators. If instrument errors occurred during calibration, troubleshoot process error alerts (System>Process Errors>Active) and rerun calibration. If you need assistance troubleshooting calibrating data, contact the Technical Solutions Center (TSC) at 1-800-441-9250. Click Next to continue.Next ,review the Acceptance Criteria Details to determine which criteria failed. Troubleshoot the calibration data, as shown here. 7Let’s Practice: Review Calibration Flags/Comments-1Let’s go through the process needed to review flags or comments generated during a calibration.From the Home screen, select the Advanced icon to display the Advanced functions menu. Go ahead and do this now.Now ,let’s go through the process needed to review flags or comments generated during a calibration. From the Home screen, select the Advanced icon to display the Advanced functions menu. Go ahead and do this now. 8Let’s Practice: Review Calibration Flags/Comments-2 From the Advanced functions menu, click the Results icon to display the test status on the Tests Status screen. Go ahead and do this now.  Next, from the Advanced functions menu, click the Results icon to display the test status on the Tests Status screen. Go ahead and do this now. 9Let’s Practice: Review Calibration Flags/Comments-2 The Test Status screen displays the test status. Click Next to continue.Enter your script here.10Let’s Practice: Review Calibration Flags/Comments-31. On the Tests Status screen, select Show Calibration Tests in the Data Filter box.2. Locate the calibration and review any flags and comments listed under the Error field to the right.3. Reject the calibration and rerun calibration with new calibrators.Click Next to continue.On the Tests Status screen, select Show Calibration Tests in the Data Filter box. Then, locate the calibration and review any flags and comments listed under the Error field to the right. Reject the calibration and rerun calibration with new calibrators. 11Let’s Practice: Review Instrument Errors-1Let’s go through the process needed to review instrument errors.From the Home screen, select the System button. Go ahead and do this now.Now let’s go through the process needed to review instrument errors. From the Home screen, select the System button. Go ahead and do this now. 12Let’s Practice: Review Instrument Errors-2On the submenu of System options, click Process Errors to view a list of active errors.Go ahead and do this now.On the submenu of System options, click Process Errors to view a list of active errors. Go ahead and do this now. 13Let’s Practice: Review Instrument Errors-3On the Process Errors screen that appears, the Active submenu displays the list of active errors.Troubleshoot the error and repeat the calibration.Click Next to continue.On the Process Errors screen that appears, the Active submenu displays the list of active errors. Troubleshoot the error and repeat the calibration. 14Review a Calibration With ErrorsWhen a calibration with an error or test report message is accepted by the user, the individual acceptance criteria are shown as Accepted and overall acceptance is shown as Reviewed on calibration printouts.Click Next to continue.When a calibration with an error or test report message is accepted by the user, the individual acceptance criteria are shown as “Accepted” and overall acceptance is shown as “Reviewed” on calibration printouts.15Cancel a Calibration Order You can cancel a calibration order if the status is Ordered or Awaiting Scheduling. If the status is Preparing Calibrators, the calibration has begun and should not be canceled. You can always reject the calibration if the results are not acceptable, as follows: 1. From the Advanced>Calibrations menu, select Calibrations by Lot. 2. Select a reagent lot. Note: For Dimension Vista® 3000T Systems, use the twin selector to designate left or right instrument. 3. From the Actions menu, select Cancel Order. Now you have learned how to use calibration troubleshooting functions.When complete, select the X in the upper-right corner to close the window and continue.You can cancel a calibration order if the status is Ordered or Awaiting Scheduling. If the status is Preparing Calibrators, the calibration has begun and should not be canceled. You can always reject the calibration if the results are not acceptable. To cancel a calibration, follow the procedure shown here. Congratulations! Now you have learned how to use calibration troubleshooting functions. After a method has been calibrated, the Order menu on the Advanced > Calibration > Calibrations by Lot screen expands to offer the following method study options: Verification, Linearity, Precision, and Analytical Sensitivity. Refer to the Dimension Vista® System Operator’s Guide, Advanced Functions, Calibration for complete procedure information about using these studies.   Method Study Options Learn about the method study options. Tab TitleTextVerification Verification    Verification of calibration tests materials with known concentrations in the same manner as patient samples to ensure accuracy of measured values throughout the reportable range. Used to satisfy government/laboratory requirements at installation, to extend a calibration interval, for method troubleshooting. Materials must be appropriate for clinical specimens assayed by that method; target values must span assay’s analytical range. The system creates the same levels/replicates per level as a calibration using onboard calibrator vials to prepare intermediate levels. Can also select cup option to use calibrators in cups. Linearity Study Linearity Study The linearity study verifies the analytical measurement range (AMR, or assay range/reportable range) during method installation. Data generated can also be used for troubleshooting. The system creates five levels and orders two replicates per level using onboard calibrator vials to prepare intermediate levels (25%, 50%, 75%). Can also select cup option to use calibrators in cups—use low/ high calibrator, or water/high calibrator, depending on method. See method IFU for required number of calibrator levels/cups. Precision Study Precision Study    The precision study verifies within-run precision (n=5) or repeatability during method installation. Data generated can be used for troubleshooting. Note: For plasma proteins, Siemens Healthcare Diagnostic QC products are used to verify within-run precision (n=5) or repeatability during method installation. The system creates two levels and orders five replicates per level using onboard calibrator vials. You can also use calibrators in cups by selecting the cup option For methods that use two calibrator levels, transfer contents of each calibrator to a cup. For methods with multiple calibrator levels, use calibrator levels that closely match analyte concentration listed in Maximum Observed Repeatability section of IFU.     Analytical Sensitivity Study Analytical Sensitivity Study   The analytical sensitivity study verifies the low limit of the analytical measurement range (AMR, or assay range/reportable range) during method installation. Data generated can also be used for troubleshooting. Pipette a zero calibrator, reagent grade water, or saline into a cup to perform the 20-replication study. The maximum recommended fill volume for a sample cup is 1.0 mL.. Ensure that there is sufficient sample in the cup—the system does not verify sufficient sample. When the order confirmation appears, the cup option is already selected.  When complete, select the X in the upper-right corner to close the window and continue.