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Dimension Vista® System Principles of Vista® Chemistry V-LYTE IMT System Online Training

Describe how ion selctive electrodes measure electrolyte concentration. Identify and describe the fuctions of key components of the IMT. Describe how the IMT System performs automatic calibrations. Describe the IMT System sample processing, fluid flow, and measurement process. This clinical laboratory training qualifies for continuing education units (CEU).

Describe how ion selective electrodes measure electrolyte concentration. Describe how the IMT System performs automatic calibrations Describe the IMT System sample processing, fluid flow, and measurement process Identify and describe the functions of key components of the IMT System, such as the V-LYTE™ Multisensor Welcome to the V-LYTE™ Integrated Multisensor Technology (IMT) System online education support tool. After completing this course, you'll be able to: Select Next to continue. The EasyLink Data Management System provides support applications that assist you with questions regarding your product. The two main support applications are the iGuide and the Onboard Help. The iGuide provides an indexed and searchable format of the Operator's Guide. The Onboard Help provides performance support for specific task related activities in the software. Integrated Multisensor Technology (IMT) allows the Dimension Vista® System to simultaneously measure Na+, K+, and Cl- concentrations in serum, plasma, and urine samples. What’s integrated? The IMT System uses a special cartridge that “integrates” four electrodes: three ion selective electrodes plus a reference electrode into a single device called the V-LYTE™ Multisensor. How does it work? The IMT System uses peristaltic action to pull samples and standards through the manifold and accurately position fluids in the V-LYTE™ Multisensor for measurement. What’s unique?The IMT System uses an alternating air-liquid flow pattern to separates samples as well as allow the V-LYTE™ Multisensor to detect when fluids cross ion selective membranes for optimal measurement. Select Next to continue.   To initialize the application, double click the RAPIDComm icon which appears on the desktop. When logging in, input your user name and password and click on the green arrow. Your User Name and password will be provided to you by your IT Department and will be configured with appropriate security levels. In the next simulation, you will learn how to login The major component of the IMT System is the V-LYTE™ Multisensor, which provides ion selective electrodes that measure the electrolytes Na+, K+, and Cl-. Let’s learn more about the V-LYTE™ Multisensor. The V-LYTE™ Integrated Multisensor uses the technology of potentiometry to measure Na+, K+, and Cl- concentrations in diluted samples, or standards. V-LYTE™ Multisensor Learn how the V-LYTE™ Multisensor works. Base ImageHotspotsText BlocksImage FileThe V-LYTE™ Integrated Multisensor comprises a ceramic sensor substrate with three ion selective electrodes and one reference electrode, an elastomer flow channel, and a cartridge housing. The V-LYTE™ Multisensor shelf life, usage, and lot number are automatically tracked by the Dimension Vista® System.The Elastomer Flow Channel separates the sample (or standard) and Salt Bridge Solution as the fluids are pulled through the IMT System to the ion selective electrodes and reference electrode for measurement. Elastomer is a rubber-like polymer.The Cartridge Housing contains slots that allow pins to come in contact with the ion selective electrodes, and holes to allow fluid to enter and exit the elastomer flow channel.The ceramic Sensor Substrate holds the Na+, K+, and Cl- ion selective electrodes and the reference electrode.The Reference Electrode measures the constant electric potential generated by Salt Bridge Solution, which is a reference solutionNa+, K+, and Cl- methods use indirect V-LYTE™ Integrated Multisensor technology (IMT). There are four electrodes used to measure electrolytes. Three of these electrodes are ion selective for sodium, potassium, and chloride. A reference electrode is also incorporated in the multisensor. Diluted sample (1:10 with V-LYTE Diluent) is positioned in the sensor and Na+, K+, and Cl- ions establish equilibrium with the electrode surface. A potential is generated proportional to the logarithm of the analyte activity in the sample. The electrical potential generated on a sample is compared to the electrical potential generated on a standard solution, and the concentration of the desired ions is calculated using the Nernst equation. For photometric, nephelometric, and LOCI™ assay methods, the Dimension Vista® System dispenses reagent and sample into cuvettes or reaction vessels for analysis. But, the IMT System is unique from these technologies... The IMT System uses a unique sampling process and air-fluid flow system to measure electrolyte concentrations in plasma, serum, or urine samples. The IMT System is located in a separate area from the other Dimension Vista® System detection systems. Let’s take a closer look at the unique features and processes of the IMT System by identifying and describing the functions of key components used during IMT System measurements.     IMT System Features, Components, and Processes Learn how the IMT system measures the electrolytes. Slide NumberText BlocksCalloutsAudio ScriptImage File1Welcome to the Virtual Chemistry Lab. Let's see how the IMT System measures the electrolytes Na+, K+, and Cl- —from start to finish. To begin, let's get familiar with key areas. Are you ready to go inside the IMT System? Select Next to continue.Note: If audio does not automatically start, select the play arrow in the top left to begin.Welcome to the virtual Chemistry Lab! Let’s see how the IMT System measures the electrolytes sodium, potassium and chloride - from start to finish. To begin, let’s get familiar with key areas. Are you ready to go inside the IMT System? Click the Next arrow, to start the tour! You can travel to this destination only in the virtual laboratory! 2IMT System Key Areas Three main areas of the IMT System include components that perform important functions. The IMT System manifold includes a sample port, where the IMT probe makes a 1:10 sample dilution, and a rotary valve, which controls air and fluids that enter the system. The IMT System pump uses peristaltic action to pull fluids through the system. The V-LYTE™ Multisensor contains ion selective electrodes and a reference electrode that measure the electrolytes. There's another feature of the IMT System that performs a very important function...select Next to continue.Select each number to view the corresponding text.CalloutsIMT System PumpIMT System ManifoldV-LYTE™ MultisensorThree main areas of the IMT System include components that perform important functions. The IMT System manifold includes a sample port, where the IMT probe makes a 1 to10 sample dilution, and a rotary valve, which controls air and fluids that enter the system. The IMT System pump uses peristaltic action to pull fluids through the system. And, the V-LYTE™ Multisensor contains ion selective electrodes and a reference electrode that measure the electrolytes. There’s another feature of the IMT System that performs a very important function… 3Air-Fluid Flow System The air-fluid flow system separates one sample from the next as standard and sample solutions are pulled into the IMT System. Here's how: As fluids are pulled into the IMT System, the rotary valve in the IMT System manifold rotates to insert air segments into the fluid stream. The alternating air-fluid pattern separates fluids and allows the V-LYTE™ Multisensor to detect when fluid crosses the electrode membranes for measurement. Select Next to continue.CalloutsSampleAirElastomer Flow ChannelDiluted SampleStd A or BThe air-fluid flow system separates one sample from the next as standard and sample solutions are pulled into the IMT System. Here’s how: As fluids are pulled into the IMT System, the rotary valve in the IMT System manifold rotates to insert air segments into the fluid stream. The alternating air-fluid pattern separates fluids and allows the V-LYTE™ Multisensor to detect when fluid crosses the electrode membranes for measurement. 4Calibrating the IMT System The IMT System must be calibrated before it can measure samples. The IMT System performs an automatic two point calibration every four hours. Two-point calibrations use Standard A, Standard B and the Salt Bridge reference solution. These reagents are stored behind Door 1 of the Dimension Vista System. Let's learn more about IMT calibrations...select Next to continue.CalloutsIMT standardsThe IMT System must be calibrated before it can measure samples. The IMT System performs an automatic two point calibration every four hours • Two-point calibrations use Standard A, Standard B and the Salt Bridge reference solution. • These reagents are stored behind Door 1 of the Dimension Vista® System. Let’s learn more about IMT calibrations… 5Two-Point Calibrations To ensure that results are accurate, the IMT System performs a two-point calibration every four hours. The two-point calibration occurs in two stages: First, the IMT System performs a peristaltic pump alignment to ensure that standards and samples are accurately positioned across electrode membranes in the V-LYTE™ Multisensor. Then, the IMT System performs a two-point calibration…let's learn more about two-point calibrations...select Next to continue.CalloutsWasteSampleVent 1Standard AStandard BVent 2To ensure that results are accurate The IMT System performs a two-point calibration every four hours. The two-point calibration occurs in two stages: First, the IMT System performs a peristaltic pump alignment to ensure that standards and samples are accurately positioned across electrode membranes in the V-LYTE™ Multisensor. Then, the IMT System performs a two-point calibration…let’s learn more about two-point calibrations… 6Two-Point Calibrations After the pump alignment, the system performs a two-point calibration by measuring Standard A and Standard B. The IMT System measures the electric potential of known concentrations of NA+, K+, and Cl- ions in Standard A and Standard B. Millivolt readings generated by NA+, K+, and Cl- ions are used to compute a conversion slope for each electrolyte. The IMT System checks to ensure that each slope falls within acceptable ranges. Select Next to continue.After the pump alignment, the system performs a two-point calibration by measuring Standard A and Standard B. Here’s how: • The IMT System measures the electric potential of known concentrations of sodium, potassium and chloride ions in Standard A and Standard B. • Millivolt readings generated by sodium, potassium and chloride ions are used to compute a conversion slope for each electrolyte. • The IMT System checks to ensure that each slope falls within acceptable ranges. 7Processing Patient Samples Now that we are familiar with the IMT System, let's learn more about key components by following a patient sample with an electrolyte test request for Na+, K+, and Cl- . To check for system drift, the IMT System automatically performs a one-point calibration using Standard A—for each electrolyte test request! Using Standard A, the IMT System measures the electric potential of known concentrations of NA+, K+, and Cl- ions and compares the readings to stored results. Readings must fall within an acceptable range. Then, the IMT probe aspirates 25μL of patient sample from an aliquot plate well...select Next to continue.CalloutsIMT ProbeAliquot PlateNow that we are familiar with the IMT System, let’s learn more about key components by following a patient sample with an electrolyte test request for sodium, potassium and chloride. To check for system drift, the IMT System automatically performs a one-point calibration using Standard Afor each electrolyte test request! Using Standard A, the IMT System measures the electric potential of known concentrations of sodium, potassium and chloride ions and compares the readings to stored results. Readings must fall within an acceptable range. Then, the IMT probe aspirates 25 microliters of patient sample from an aliquot plate well… 8Diluting Samples 1:10 The IMT probe aspirates diluent from the sample port to automatically make a 1:10 sample dilution—this happens inside the probe! The diluted sample is mixed as the IMT probe dispenses the sample fluid into the empty and rinsed IMT sample port. Select Next to continue.CalloutsIMT Sample PortThe IMT probe aspirates diluent from the sample port to automatically make a 1 to 10 sample dilutionthis happens inside the probe! The diluted sample is mixed as the IMT probe dispenses the sample fluid into the empty and rinsed IMT sample port. 9Pulling Fluids through the Manifold The IMT System pump uses peristaltic action to start pull diluted sample through the manifold. Select Next to continue.CalloutsIMT System PumpThe IMT System pump uses peristaltic action to pull diluted sample through the manifold.10Inserting Air Segments As soon as the IMT System Pump pulls fluid through the system, the rotary valve in the IMT System manifold rotates to insert air segments into the fluid stream to create the characteristic air-fluid flow pattern in the manifold channels. Select Next to continue.CalloutsIMT System ManifoldRotary ValveAs soon as the IMT System Pump pulls fluids through the system, the rotary valve in the IMT System manifold also goes to work. The rotary valve rotates to insert air segments into the fluid stream to create the characteristic air-fluid pattern in the manifold channels. 11Pulling Fluids through the Manifold The IMT System pump pulls the diluted sample—now in the air-fluid flow pattern—through the manifold to the V-LYTE™ Multisensor. Select Next to continue.CalloutsSampleAirElastomer Flow ChannelDiluted SampleStd A or BThe IMT System pump pulls the diluted samplenow in the air-fluid flow patternthrough the manifold and all the way to the V-LYTE™ Multisensor.12Reading Electric Potential across Membranes The IMT System takes electrolyte readings when the sample fluid is optimally positioned across the ion selective electrodes in the V-LYTE™ Multisensor. The electrodes measure the electric potential generated by the Na+, K+, and Cl- ions in the sample as the ions cross highly sensitive membranes in each of the electrodes. The electric potential is measured in millivolts, which are converted into numeric results. Select Next to continue.Slide QuestionAnswer TextDid you know?Each electrolyte sample cycle takes only 21.6 seconds.CalloutsV-LYTE™ Integrated MultisensorReference ElectrodeSensor SubstrateIon Selective ElectrodesElastomer Flow ChannelCartridge HousingThe IMT System takes electrolyte readings when the sample fluid is optimally positioned across the ion selective electrodes in the V-LYTE™ Multisensor. Each electrode measures the electric potential generated by the sodium, potassium and chloride ions in the sample as the ions cross highly sensitive membranes in each electrode. The electric potential is measured in millivolts, which are converted into numeric results. 13Discarding the diluted sample After the readings are obtained, the IMT System discards the diluted sample to liquid waste. Select Next to continue.After the readings are obtained, the IMT System discards the diluted sample to liquid waste.14Auto-cleaning the IMT System The IMT System performs an automated cleaning procedure nightly to reduce protein buildup within the system—it takes only five minutes. Select Next to continue.CalloutsIMT SystemThe IMT System performs an automated nightly cleaning to reduce protein buildup within the systemit takes only five minutes!15Running a Dilution Check A dilution check: Verifies the accuracy of the 1:10 dilution Should be performed every 90 days Uses V-LYTE™ Dilution Check solution with known concentrations of Na+ and K+ Select Next to continue.Run a dilution check using V-LYTE™ Dilution Check solution every 90 days to verify the accuracy of the 1 to 10 dilution. V-LYTE™ Dilution Check solution contains known concentrations of sodium and potassium. 16Summary You have just learned about many features and processes of the IMT System by identifying and describing the functions of key components used during IMT System measurements.When complete, select the X in the upper-right corner to close the window and continue.Congratulations! You’ve just learned about many features and processes of the IMT System by identifying and describing the functions of key components used during IMT System measurements. Congratulations. You’ve completed the V-LYTE™ IMT System Online Education Support Tool. In this course, you’ve been introduced to many features, components, and processes of the IMT System, which are summarized below. Describe how ion selective electrodes measure electrolyte concentration Na+, K+, and Cl- Ion Selective Electrodes (ISEs) measure the electric potential generated by "selected" ions in a diluted sample (or standard) as the ions cross highly sensitive membranes in each electrode. These readings are compared to the constant electric potential generated by Salt Bridge Solution, which is measured by the reference electrode. The difference in conductivity between ISE and reference electrode measurements is proportional to the concentration of electrolyte in the sample (or standard). Identify and describe the functions of key components of the IMT System The IMT System manifold includes a sample port and rotary valve. The IMT probe dispenses diluted sample into the IMT port. The rotary valve rotates to insert air segments into the fluid stream to create the characteristic air-fluid pattern in the manifold channels. The IMT System pump uses peristaltic action to pull fluids through the manifold. The alternating air-fluid flow system separates one sample from the next and allows the V-LYTE™ Multisensor to detect when fluid crosses the electrode membranes for measurement. Integrated Multisensor Technology (IMT) “integrates” three ion selective electrodes plus a reference electrode into a single device called the V-LYTE™ Multisensor. Describe how the IMT System performs calibrations Two-Point Calibration: After a pump alignment, the IMT System performs a two-point calibration by measuring the electric potential of known concentrations of NA+, K+, and Cl- ions in both Standard A and Standard B. Millivolt readings generated by NA+, K+, and Cl- ions are used to compute a conversion slope for each electrolyte. The IMT System verifies that each slope falls within acceptable ranges. The IMT System must be calibrated using a two-point calibration AFTER a pump alignment and BEFORE running a dilution check. Note: Standards A and B are already prepared as 1:10 dilutions—no further dilutions of the standards by the IMT System are necessary! Describe the IMT sampling, fluid flow, and measurement process One-Point Calibration check: For each electrolyte test request, the IMT System automatically performs a one-point calibration to check for system drift by measuring the electric potential of known concentrations of NA+, K+, and Cl- ions in Standard A and comparing the readings to stored results. Readings must fall within an acceptable range. The IMT probe aspirates 25 µL of serum, plasma, or urine and then makes a 1:10 dilution. The IMT probe then dispenses the diluted sample into the IMT port. As sample or standard is pulled into the IMT System via the peristaltic action of the IMT System pump, the rotary valve rotates to insert air segments into the fluid stream. The alternating air-fluid pattern separates fluids and allows the V-LYTE™ Multisensor to detect when fluid crosses the ion selective electrode membranes for measurement. After the readings are obtained, the IMT System discards the diluted sample to liquid waste. Note: Run a dilution check using V-LYTE™ Dilution Check solution, which contains known concentrations of Na+ and K+, every 90 days to verify the accuracy of the 1:10 dilution. Be sure to perform a two-point calibration, first. Select Next to continue.