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Dimension Vista® System V-LYTE® IMT Online Training

The (Integrated Multisensor Technology) IMT System uses ion selective electrodes to measure electrolytes in serum, plasma, and urine samples. This clinical laboratory training qualifies for continuing education units (CEU).

RAPIDComm® will send results to a hospital or laboratory information system. The hospital system is displayed in the Hospital Connections tab of the Set up Patient Sample Validation window. This option is defined at the Setup Hospital Connections window accessed from the System menu. When the Query for Patient Demographics during automatic validation option is disabled, patient demographic requests are not initiated during automatic patient sample validation. During manual validation for blood gas results, operators can request patient demographics, regardless of the option setting.  When the patient query is enabled for Blood Gas, the program queries the hospital system during both automatic and manual validation of patient results. Welcome to the Dimension Vista® System V-LYTE® IMT System Training.  The (Integrated Multisensor Technology) IMT System uses ion selective electrodes to measure electrolytes in serum, plasma, and urine samples.  Electrolytes measured by the IMT System include:   Na+   K+   Cl- Select Next to continue.    Describe basic troubleshooting steps Describe flow of V-LYTE® IMT Fluids through the system Identify components of the V-LYTE® IMT Module Identify maintenance procedures After successfully completing the course, you will be able to: Select Next to continue. Congratulations. You have completed the Dimension Vista® System V-LYTE® IMT System Online Training course. Listed below are the key points that have been presented.  Take time to review the material as needed before proceeding to the final quiz.  Overview of the V-LYTE® IMT System: What is the V-LYTE® IMT System? Integrated Multisensor Technology (IMT) allows the Dimension Vista® System to simultaneously measure Na+, K+, and Cl- concentrations in serum, plasma, and urine samples. What’s integrated? The IMT System uses a special cartridge that “integrates” four electrodes: three ion selective electrodes plus a reference electrode into a single device called the V-LYTE® Multisensor. How does it work? The IMT System uses peristaltic action to pull samples and standards through the manifold and accurately position fluids in the V-LYTE® Multisensor for measurement. What is unique? The IMT System uses an alternating air-liquid flow pattern to separates samples as well as allow the V-LYTE® Multisensor to detect when fluids cross ion selective membranes for optimal measurement. Identify components of the V-LYTE® IMT System:There are various components of the IMT Module that all work together to ensure the successful measurement of electrolytes in patient samples.    The IMT System Manifold (sample port, rotary valve, and tubing connections) The IMT Probe makes a 1:10 dilution of the sample in the sample port and the Rotary valve controls the air and fluids that enter the system. The IMT System pump uses peristaltic action to pull fluids through the system. The IMT Multisensor contains ion selective electrodes and a reference electrode that measure the electrolytes. Consumables of the IMT System include: Standard B/Salt Bridge-Used in performing a two-point calibration and provides a constant reference at the reference electrode. Standard A-Used in performing a two-point calibration, a one-point offset check during each assay and used to rinse the sample port.  V-LYTE® Diluent-Used to dilute the samples 1:10. The IMT Probe must be paused prior to replacing this fluid on the system.  If it is the only fluid replaced, it does NOT require a calibration.  V-LYTE® Multisensor-Contains the Na, K, and Cl reference electrodes. Must be replaced every 14 days of use, after 2000 samples (whichever comes first), and following an Advanced IMT cleaning. Describe the flow of V-LYTE® IMT fluids through the system: The V-LYTE® IMT System uses peristaltic action to pull fluids from the rotary valve into the V-LYTE® IMT Multisensor for testing.  Understanding and identifying how the fluids flow through the system will assist you in running the system efficiently.  Once the diluted sample (1:10 with V-LYTE® Diluent) is positioned in the sensor, the Na+, K+, and Cl- ions establish equilibrium with the electrode surface. The potential generated is proportional to the logarithm of the analyte activity in the sample.  The electrical potential generated on a sample is compared to the electrical potential generated on a standard solution. Identify maintenance procedures for the V-LYTE® IMT System:  Maintenance procedures ensure the system is clean, and operating efficiently.  This is accomplished by cleaning various components of the system to remove any dried fluid build-up and to maintain proper flow rate of the Standards and samples. Routine IMT Clean-Performed automatically each day during Off-Peak Activities Inspect and clean the IMT module-Performed during weekly maintenance Inspect the Peristaltic pump tubing-Performed during weekly maintenance Perform an Advanced IMT Clean Describe basic troubleshooting steps of the V-LYTE® IMT System: The V-LYTE® IMT System is a closed indirect system of measurement.  This requires the system to have proper fluid flow. When fluids are not flowing properly, the system will fail to calibrate.  The alert displayed in the system status area will be red and display "IMT Failed to Calibrate". This error is generated when the system attempts a calibration.  The Dimension Vista® IMT System performs a two-point automatic calibration in duplicate every 4 hours. In addition, the system will routinely perform a one-point calibration check with each sample measurement.  Auto-calibration occurs after power-on, with the changing of standards A, B/Salt Bridge, or a sensor and when the system software is reset.  Select Next to continue. What is the V-LYTE® IMT System? The IMT module allows the Dimension Vista® System to simultaneously measure Na+, K+, and Cl- concentrations in serum, plasma, and urine samples. What is integrated? The IMT System uses a special cartridge that “integrates” four electrodes: three ion selective electrodes plus a reference electrode into a single device called the V-LYTE® Multisensor. How does it work? The IMT System uses peristaltic action to pull samples and standards through the manifold and accurately position fluids in the V-LYTE® Multisensor for measurement. What is unique? The IMT System uses an alternating air-liquid flow pattern to separates samples as well as allow the V-LYTE® Multisensor to detect when fluids cross ion selective membranes for optimal measurement. Select Next to continue.        There are various components of the IMT Module that all work together to ensure the successful measurement of electrolytes in patient samples.     IMT System Manifold (includes the sample port, rotary valve, and other components)    Peristaltic Pump    V-LYTE® Multisensor   V-LYTE® IMT Module Components Learn about the V-LYTE® System Components. Base ImageHotspotsText BlocksImage File V-LYTE® Multisensor: The IMT Multisensor contains ion selective electrodes and a reference electrode that measure the electrolytes.  Peristaltic Pump: The IMT System pump uses peristaltic action to pull fluids through the system.  The pump tubing is inspected during monthly maintenance.  Sample Port: The IMT Probe makes a 1:10 dilution of the sample with V-LYTE® diluent.  The sample port is inspected for salt crystal build up and cleaned if needed during weekly maintenance.  Manifold: The IMT System manifold includes a sample port, a rotary valve, and tubing connections.  Rotary Valve: The IMT System rotary valve controls air and fluids that enter the system.  As fluids are pulled into the IMT System, the rotary valve rotates to insert air segments into the system.  V-LYTE® IMT System Consumables Learn about the V-LYTE® System Consumables Tab TitleTextStandard B/Salt Bridge Standard B is used in performing a two-point calibration Salt Bridge provides a constant reference potential at the reference electrode  The system performs a calibration when replaced Note: The IMT consumables do not have reservoirs like other system bulk consumables.  The IMT Consumables screen displays a picture of each consumable container and shows the current lot and percentage of lot remaining.  Standard A Used in performing a two-point calibration Used for a one-point offset check during each assay Used in the Sample Port rinse All sensors kept in contact with Standard A during standby periods The system performs a calibration when replaced Note: The IMT consumables do not have reservoirs like other system bulk consumables.  The IMT Consumables screen displays a picture of each consumable container and shows the current lot and percentage of lot remaining.  V-LYTE® Diluent Used to dilute all samples at a 1:10 ratio Must pause the IMT probe before replacing fluid The system does NOT perform calibration if it is the only fluid replaced Note: The IMT consumables do not have reservoirs like other system bulk consumables.  The IMT Consumables screen displays a picture of each consumable container and shows the current lot and percentage of lot remaining.  V-LYTE® Multisensor Disposable cartridge Contains the Na, K, and Cl reference electrodes Must be replaced every 14 days or after 2000 samples (whichever comes first) and following an Advanced IMT Cleaning Note:  The IMT Consumables screen displays a picture of each consumable container and shows the current lot and number of days/tests remaining for lot. When complete, select the X in the upper-right corner to close the window and continue.    Fluid Flow in the V-LYTE® IMT System:  The IMT system relies on the proper flow of fluids through the system.  It begins with the sample dilution in the sample port to the proper positioning of fluids in the  V-LYTE® multisensor.  Maintaining this flow is critical to ensure the IMT system runs effectively on the Dimension Vista® System.  IMT Fluid Flow Learn about the flow of fluids in the V-LYTE® IMT system. Slide NumberText BlocksCalloutsAudio ScriptImage File1 As the Dimension Vista System prepares to run an IMT calibration, fluid movement through the IMT module is initiated. As fluids are processed, each one has a unique role that it plays in the V-LYTE IMT system.    Select Next to continue. Note: If audio does not automatically start, select the play arrow in the top left to begin.As the Dimension Vista System prepares to run an IMT calibration, fluid movement through the IMT module is initiated. As fluids are processed, each one has a unique role that it plays in the V-LYTE IMT system. 2 The V-LYTE bulk fluids are stored on the inside of the left hand instrument door.  Even though it is not possible to view the entire flow of fluids as they travel through the system, each of the tubing connections for the fluids are color coded to assist locating the appropriate connections when needed.  When the calibration is initiated Standard A will begin flowing from Fluid #2 up to the Rotary Valve Manifold.  Select Next to continue. Select each number to view the corresponding text.CalloutsStandard AStandard B/Salt BridgeV-LYTE DiluentThe V-LYTE bulk fluids are stored on the inside of the left hand instrument door. Even though it is not possible to view the entire flow of fluids as they travel through the system, each of the tubing connections for the fluids are color coded to assist locating the appropriate connections when needed. When the calibration is initiated Standard A will begin flowing from Fluid #2 up to the Rotary Valve Manifold.3 After the fluid enters the rotary valve manifold, it will move through the rotary valve, and enter the V-LYTE Multisensor (flow is left to right). Once through the sensor, the fluid will move through the peristaltic pump out to the waste. Select Next to continue. CalloutsRotary ValveV-LYTE MultisensorPeristaltic PumpOnce the fluid enters the rotary valve manifold, it will move through the rotary valve, and enter the V-LYTE Multisensor (flow is left to right). Once through the sensor, the fluid will move through the peristaltic pump, out to the waste.4 Next the system will use air to separate the different fluids.  To do this, the rotary valve will rotate to allow air to enter the system. Then the air will follow the same path as the fluids: flowing through the sensor, to the peristaltic pump, and then to the waste. Select Next to continue. Next the system will use air to separate the different fluids. To do this, the rotary valve will rotate to allow air to enter the system. Then the air will follow the same path as the fluids: flowing through the sensor, to the peristaltic pump, and then to the waste.5Now Standard B will flow through the module. This is the second point of the two point calibration using Standards A and B. Select Next to continue. Callouts Standard A Standard B/Salt BridgeV-LYTE DiluentNow Standard B will flow through the module. This is the second point of the two point calibration using Standards A and B.6 Once in the Rotary Valve Manifold, Standard B follows a similar path as Standard A through the system. Air is then introduced into the system to separate the different fluids.   Select Next to continue. CalloutsRotary Valve ManifoldV-LYTE Multisensor Peristaltic Pump Once in the Rotary Valve Manifold, Standard B follows a similar path as Standard A through the system. Air is introduced into the system to separate the different fluids.7Now Standard A flows through the module again to ensure an accurate calibration has occurred. Select Next to continue.  CalloutsStandard AStandard B/Salt BridgeV-LYTE DiluentNow Standard A flows through the module again to ensure an accurate calibration has occurred.8During the process of Standard A and B flowing through the system, Salt Bridge is flowing at the same time. Select Next to continue. CalloutsStandard AStandard B/Salt BridgeV-LYTE DiluentDuring the process of Standard A and B flowing through the system, Salt Bridge is flowing at the same time. 9The Salt Bridge solution will not go through the Rotary Valve.  Instead the Salt Bridge solution will flow directly to the sensor from Rotary Valve Manifold.  This calibration flow of fluids occurs every 4 hours, every time a consumable is replaced, or when the temperature changes ±2º. During the processing of samples, the flow of fluids is slightly different, let’s take a look at the flow of samples. Select Next to continue.CalloutsRotary Valve Manifold V-LYTE Multisensor Peristaltic PumpThe Salt Bridge solution will not go through the Rotary Valve. Instead the Salt Bridge solution will flow directly to the sensor from Rotary Valve Manifold. This calibration flow of fluids occurs every 4 hours, every time a consumable is replaced, or when the temperature changes ±2º. During the processing of samples, the flow of fluids is slightly different, let’s take a look at the flow of samples. 10The IMT probe is responsible for making a 1 to10 dilution of each sample.  This process begins as the probe is filled with 225 µL of V-LYTE diluent.  This diluent is delivered from the consumable on the door up to the probe. Select Next to continue.  Callouts IMT Probe The IMT probe is responsible for making a 1 to10 dilution of each sample. This process begins as the probe is filled with 225 microliters of V-LYTE diluent. This diluent is delivered from the consumable on the door up to the probe.11 The probe then moves to the aliquot plate and aspirates 25 µL of sample from the appropriate well. Select Next to continue. The probe then moves to the aliquot plate and aspirates 25 microliters of sample from the appropriate well. 12 Next, the probe returns to the IMT sample port and dispenses 250 µL of sample/diluent into the port and the sample is mixed. Select Next to continue. Next, the probe returns tothe IMT sample port and dispenses 250 microliters of sample/diluent into the port and the sample is mixed. 13This diluted sample remains in the port until it is drawn out through the rotary valve.   (The rotary valve rotates to move into position and allow the movement of the appropriate fluids or air into the system.)  As the sample is drawn out of the port, it is segmented with a series of two air slugs.  Select Next to continue.  CalloutsAliquot plate Rotary Valve Manifold This diluted sample remains in the port until it is drawn out through the rotary valve. The rotary valve rotates to move into position and allow the movement of the appropriate fluids or air into the system. As the sample is drawn out of the port, it is segmented with a series of two air slugs. 14The sample is then drawn into the IMT sensor.  At the same time, the sample port is rinsed with Standard A and the rinse is removed to the vacuum waste by the rotary valve.  The IMT Peristaltic pump is responsible for the movement of air and sample segments into the measuring channel of the IMT sensor.  The proper positioning of the sample is verified through two separate conductivity readings; the first confirms the previous sample was removed by recognizing the presence of the new air slug and the second reading confirms that a new sample is present.  When complete, select the X in the upper-right corner to close the window and continue. The sample is then drawn into the IMT sensor. At the same time, the sample port is rinsed with Standard A and the rinse is removed to the vacuum waste by the rotary valve. The IMT Peristaltic pump is responsible for the movement of air and sample segments into the measuring channel of the IMT sensor. The proper positioning of the sample is verified through two separate conductivity readings; the first confirms the previous sample was removed by recognizing the presence of the new air slug and the second reading confirms that a new sample is present. Routine Maintenance Tasks of the IMT System include:    Routine IMT Clean – performed daily during Off-Peak Activities    Inspect IMT Module – performed during weekly maintenance    Inspect Peristaltic Pump Tubing – performed during monthly maintenance    Advanced IMT Clean – performed as needed    Note: These procedures are instrument specific and must be performed on both the left and right sides of a Dimension Vista® 1000T/3000T System.  Inspect IMT Module Learn how to inspect the IMT module weekly. Checklist TitleChecklist TypeChecklist ContentPause the instrumentHTML   Select Pause under the Operation icon to pause the instrument.   Select each checkbox to learn how to inspect and clean the IMT module.   Locate the IMT ModuleHTML   Release the center interlock using an appropriate tool.  Lift the left and center instrument lids and locate the IMT module area.   Release the Aliquot HolderHTML   Gently lift up the rectangular latch on each side of the aliquot plate holder to release the aliquot plate holder cover.  Set the cover aside on a flat surface.  Inspect and clean sample portHTML    Locate the IMT Sample Port.  If salt crystals are present, clean as needed using a damp cloth.   Reset the instrumentHTML     Reinstall the aliquot plate holder cover, close the instrument lids, and reset the instrument.  When complete, select the X in the upper right corner to close the window and continue.    Inspect Peristaltic Pump Tubing Learn how to inspect the Peristaltic Pump monthly. Checklist TitleChecklist TypeChecklist ContentPause the instrumentHTML   Select Pause under the Operation menu to pause the instrument.   Select each checkbox to learn more about how to inspect the IMT Peristaltic Pump tubing.     Locate the IMT Peristaltic PumpHTML    Open the instrument lids to locate the IMT Peristaltic Pump which is located near the sample loading area.   Inspect TubingHTML   Lift the pump lid and inspect the tubing for flat areas.  If tubing is free of any flat areas, reseat the tubing and close the pump lid.  Note: If the tubing needs to be replaced, refer to the iGuide for complete details on how to replace the IMT Peristaltic Pump tubing.  Flattening of the IMT Pump Tubing could adversely affect the IMT pump rate.   Reset the InstrumentHTML   Close the instrument lids and reset the instrument.  When complete, select the X in the upper right corner to close the window and continue.   Advanced IMT Clean Learn how to perform an Advanced IMT Clean Slide NumberText BlocksCalloutsAudio ScriptImage File1 The IMT section of the Current Activity screen displays Advanced IMT Clean details including: Days to Next Clean Average Fluid Delta Sample Port Drain Time The Dimension Vista system will notify the operator when it is time for an Advanced IMT Clean or can be initiated at any time to assist in V-LYTE IMT system troubleshooting.   Select Next to continue. Note: If audio does not automatically start, select the play arrow in the top left to begin.The Advanced IMT clean is performed as needed on the Dimension Vista System. The software monitors the number of days between cleans, sample port drain time and the average fluid delta to assess when the next Advanced clean will need to be completed. An Advanced IMT clean is initiated by the operator when notified by the Dimension Vista Systme or at any time to assist in V-LYTE IMT system troubleshooting. Select Next to continue. 2 When an Advanced IMT Clean is required the operator will be notified in both the Current Activity > IMT screen and in the IMT Consumables > Replace IMT Consumables screen.     Select Run Advanced IMT Clean to start the process.   Note: The Advanced IMT Clean should be coordinated with the next sensor replacement. The Dimension Vista system will notify the operator when an Advaced IMT clean is required. The Advanced clean can be started from the replace IMT Consumables screen. This task should be coordinated with the next V-Lyte multisensor replacement as the Advanced clean bleaches the IMT system, rendering the sensor unusable. Once the Run Advanced IMT Clean button is selected the Advanced IMT cleaning process will start automatically. Select Run Advanced IMT Clean to start the process. 3 Select the finish button to close the Maintain IMT dialog bog. Replace the V-LYTE Multisensor once the IMT Status indicates that the Advanced Clean is complete.   Select Next to continue. Callouts IMT Status area Select Finish from the Maintain IMT dialog box to close the screen. The system will continue processing all samples except electrolytes. When the IMT Status area indicates the clean cycle is complete, change the sensor. Select Next to continue. 4 An Advanced IMT Clean can also be scheduled by the operator at anytime to assist in V-LYTE IMT system troubleshooting.  To initate an Advanced clean for troubleshooting: Navigate to System > IMT Calibration Select Maintain IMT The Advanced IMT clean function can also be started by the operator at anytime to assist in troubleshooting. To initiate an Advanced clean for troubleshooting, navigate to the IMT Calibration screen and select Maintain IMT. Select the Maintain IMT activity button to continue. 5 Select the IMT Advanced Clean radio button and select Next to continue. Select the IMT Advanced Clean option and select Next in the Maintain IMT screen to continue. 6 The Advanced IMT Clean will launch automatically.   Select the finish button to close the Maintain IMT dialog bog.   Replace the V-LYTE Multisensor once the IMT Status indicates that the Advanced Clean is complete.   Select Next to continue. Callouts IMT Status area The Advanced IMT Cleaning process will start automatically. Select Finish from the Maintain IMT dialog box to close the screen. The system will continue processing all samples except electrolytes. When the IMT Status area indicates the clean cycle is complete, change the sensor. Select Next to continue. 7 You have just viewed how to:   Identify when the Dimension Vista System requires an Advanced IMT Clean Initiate an Advanced IMT Clean following system notification Initiate an Advanced IMT Clean to assist in V-LYTE IMT system troubleshooting When complete, select the X in the upper-right corner to close the window and continue. You have just viewed how to identify when the Dimension Vista System requires an Advanced IMT clean and how to initiate an Advanced IMT clean following system notification or when troubleshooting the V-LYTE IMT system. A red system alert stating "IMT Failed to Calibrate" will be generated if the fluids in the IMT system are not flowing properly.  This error is generated following a failed calibration. IMT Calibration: A two-point automatic calibration is performed in duplicate every 4 hours A one-point calibration check is performed with each sample measurement Auto-calibration occurs after the instrument power is turned on, standards A, B/Salt Bridge, or sensor are changed, every 175 samples, when the temperature changes more than 2°C, and when the system software is reset   Basic steps that can be taken to troubleshoot the V-LYTE® System include: Check and/or replace IMT System fluids Perform Advanced IMT Clean Inspect and/or replace IMT Pump tubing Replace V-LYTE® sensor Replace IMT Probe  Download and print a PDF about IMT System Troubleshooting.   IMT Dilution Check Learn how to perform an IMT Dilution Check. Slide NumberText BlocksCalloutsAudio ScriptImage File1 IMT Dilution Check: Used to monitor and adjust the dilution ratio on the V-LYTE® IMT System.  An IMT Dilution Check must be performed in each of the following scenarios: After replacing the IMT Probe After replacing the IMT peristaltic pump tubing If V-LYTE® QC is out of limits Navigate to Advanced > IMT to perform Dilution Check Note: This procedure is instrument specific and must be performed on both the left and right sides of a Dimension Vista® 1000T/3000T System. Note: If audio does not automatically start, select the play arrow in the top left to begin.A dilution check is used to verify the performance of the Aliquotter/IMT probe and to assess the quality of the IMT probe mix. This procedure must be performed in each of the following scenarios; after replacing the IMT Probe, after replacing the IMT peristaltic tubing, and if the V-LYTE QC is out of limits. To access the IMT Dilution Check screen, we need to navigate to the Advanced software. In this example, we have already logged on with Admin rights, so select the Advanced icon on the screen now. 2 IMT Dilution Check: In Advanced, select the IMT icon. Select the IMT icon under Advanced. 3 IMT Dilution Check: Select IMT Dilution Check from the IMT drop down menu.    Next, select IMT Dilution Check under the IMT menu. 4 IMT Dilution Check: Fill 3 sample cups with 300µL of V-LYTE® Dilution Check solution Refer to the iGuide for specific directions to safely open the Dilution Check ampoule Transfer content to three sample cups Note: Be sure to follow the laboratory's guidelines for sharps disposal.  Select Next to continue.  The V-LYTE® Dilution Check solution contains a known concentration of both sodium and potassium. Dispense 300 microliters of V-LYTE® dilution check solution into three separate sample cups. When you are finished with the V-LYTE® Dilution check ampoule, follow your laboratory's guidelines for proper sharps disposal. 5 IMT Dilution Check: Follow on screen instructions to position sample cups appropriately in the sample rack Scan the rack barcode Select Next to continue.    Onscreen instructions display the appropriate position of the sample cups in the sample rack. When properly positioned, scan the rack barcode. 6 IMT Dilution Check: Select the Run Dilution Check button on the IMT Dilution Check screen.   Next, select the Run Dilution Check button on the screen. 7 IMT Dilution Check: Load the rack in the sample input lane.  Select Next to continue.    And load the rack in the sample input lane. 8 IMT Dilution Check:   Review the completed IMT Dilution Check report Review any recommendations that appear Select Print to print the report Select Next to continue.   Tip:  Refer to the iGuide for more information on Troubleshooting an IMT Dilution Check that has results outside of the acceptable limits.  Contact the Siemens Technical Solutions Center for further assistance. Once the Dilution check is complete, review the onscreen results paying close attention to any recommendations that appear. The iGuide contains detailed procedures on how to resolve a failed IMT Dilution check and if further assistance is required, contact the Siemens Technical Solutions Center. 9 You have just viewed how to:    Identify when an IMT Dilution Check is indicated List the necessary steps to perform an IMT Dilution Check Locate available resources to troubleshoot a failed IMT Dilution Check When complete, select the X in the upper-right corner to close the window and continue. You have just viewed when to perform an IMT Dilution Check, the steps to perform a dilution check and the available resources to troubleshoot a failed test on the system. Prime IMT System Learn how to prime the V-LYTE IMT System. Slide NumberText BlocksCalloutsAudio ScriptImage File1 Priming the V-LYTE® System:   Navigate to System > IMT Calibration   Select Maintain IMT on the IMT Calibration screen Note: This procedure is instrument specific and must be performed on both the left and right sides of a Dimension Vista® 1000T/3000T System.    Note: If audio does not automatically start, select the play arrow in the top left to begin.When an IMT Calibration fails, it may be due to an improper flow of fluids through the system. A good first step to troubleshooting a failed IMT Calibration is to Prime the IMT fluids after it is confirmed an adequate supply of each fluid is onboard the system. In this example, select the Maintain IMT button to continue. 2 Priming the V-LYTE® System: Select the Prime IMT radio button on the Maintain IMT dialog box then select Next. After selecting the Prime IMT radio button on the Maintain IMT dialog box, the Next button is selected to continue. Select the Next button now. 3 Priming the V-LYTE® System: Follow screen prompts to Prime IMT fluids Watch fluid flow through the IMT sample port Select Finish when prime is complete Follow instructions on the screen to Prime the IMT fluids. The IMT Probe must be paused prior to priming the V-LYTE® diluent. When complete, select the Finish button on the screen to complete the priming procedure. In this example, select the Finish button now. 4You have just viewed how to:  Navigate through the software to maintain the IMT system Prime IMT fluids When complete, select the X in the upper-right corner to close the window and continue.You have just viewed the steps necessary to Prime the IMT fluids on the Dimension Vista System.