General Laboratory: Blood Gas: Sample Collection and Proper Handling: Arterial Line From the Radial Artery Using a Syringe

Instructional video about how to collect a blood gas sample from an arterial line from the radial artery using a syringe.

Blood gas sample collection and proper handling. This is an instructional video about how to collect a blood gas sample from an arterial line from the radial artery and the proper handling of the sample immediately after obtaining the blood sample and just prior to performing the blood gas analysis. Follow your local hospital procedures to prepare the patient and collection site for obtaining the blood sample. The insertion of an indwelling arterial catheter is one way for the continuous monitoring of blood gas results. In an acutely ill patient, the radial artery is generally the artery of choice for arterial cannulation. Blood gas analysis is performed to test vitals in critically ill patients. Blood gas analysis gives information on pH, the gases, electrolytes, metabolites, total hemoglobin, Co oximetry, and neonatal total bilirubin. Ensure you have all the materials you need to draw the patient sample, including an empty waste syringe, to clear the arterial line. A heparinized syringe with a filter cap and an alcohol wipe. The syringe used for blood gas analysis should include dry electrolyte balanced lithium heparin as the preferred anticoagulant in a concentration of approximately 23 international units per milliliter of blood. Before obtaining the sample from the A line, confirm the patient's identity. If there has been a recent adjustment to the ventilator, wait approximately 20 to 30 minutes before obtaining the blood sample from the patient. Clean the stopcock sample port. Do not use a cleaning wipe that contains Quanta, Neri ammonium substances, such as benzalkonium as it may affect the electrolyte parameters reported, particularly sodium attached the waste syringe to the a line sample port and position the stopcock to remove fluid from the arterial line and some blood from the line. The amount of fluid that needs to be withdrawn varies with the arterial system used. Reposition the stopcock to the closed position. Remove and discard the waste syringe. Attach the heparinized syringe to the a line sample port. Reposition the stopcock, and withdraw the desired volume of blood from the patient. Once the desired volume of blood is obtained, return the stopcock to the closed position and remove the syringe from the sample port attached. The filter cap on the lower tip of the syringe and hold the syringe vertically and gently tap the syringe so that air bubbles are forced to the top. Expel any air bubbles into the filter cap. Mix the sample thoroughly by rotating your wrist back and forth for a minimum of 20 seconds. This helps to dissolve the heparin and minimize clot formation in the syringe. Label the syringe with the patient ID. Flush the arterial line and clear the line of blood according to hospital protocol and immediately transport the sample to a Siemens Healthineers blood gas system. Follow a two step mixing process. First, by rotating your wrist back and forth and then rolling the syringe between your hands about 10 times, remove the cap from the syringe and expelled. The first few drops of blood into a gauze pad. Present the sample to the analyzer and start analysis. Recognize that a sample containing blood clots should not be used on the blood gas analyzer because it can affect not only the accuracy of the patient results, but also negatively impact the operation of the blood gas. Analyzer the patient's blood gas results will be ready within 60 seconds. Remove the syringe from the analyzer. Reattached the filter cap and the bubble the sample. If you need to repeat the test, remix the sample prior to analysis. According to the C LSI guidelines, blood gas testing should be completed within 10 minutes and not longer than 30 minutes after drawing the sample. If testing is delayed longer than 30 minutes, samples should be placed in an ice slurry and will require additional mixing prior to analysis. Dispose of the syringe according to the hospital guidelines. Proper sample handling, mixing and time to analysis helps to ensure. Accurate patient results.

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