IMMULITE® 1000 Immunoassay System Sample Processing Online Training

Welcome to the IMMULITE® 1000 Immunoassay System Sample Processing Online Training course.  This course introduces you to: Loading kit components on the system Processing patient samples, including running dilutions Reviewing results Select Next to continue. A Real Drawback objection is when the customer perceives a need for something that your solution does not offer or perceives that a competitive solution is offered at a lower price.  The strategy for handling a Real Drawback includes showing the customer the big picture to help explain why your solution will best meet their overall needs.  Select the link below to learn about the Real Drawbacks you may encounter in competitive situations. To access the Review screen to view results, select Review from the horizontal toolbar. A blank Review screen displays. Results will appear on the screen when a time frame is selected. Review Screen Components Learn more about the Review screen. Base ImageHotspotsText BlocksImage File Result Type In this area of the screen, select the type of result you would like to view: All Patient Adjustor Control Verifier (for calibration verifier)  Date and Time Range This area of the screen is used to set the time frame for the results that will be viewed on the screen. The default is the current date. When the Date and Time button is selected, the Select Date Time Range window opens.  You can select to view a specific range, today only, or all results.  After making your selection, click the OK button.  Search When the Search button is selected, the Search window opens.  This option allows you to search for a particular result. The Search window varies depending on the record type selected in the options area: patient, control, adjustor, or calibration verifier.  The Search button is not available if the All option is selected.    Results   Results are listed on the screen for one sample at a time.  Previous / Next  Use the Previous and Next buttons to scroll through the results, viewing the results for one sample at a time.  Print The Print button is used to print the results for the sample currently displayed on the screen.  Sample Cup Identification This area of the screen listed the identification information for the sample cup whose results are currently shown. Distinguish between the operating modes Identify kit components List the steps to process onboard dilutions List the steps to process patient samples Navigate the Review screen Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations. You have completed the IMMULITE 1000 Immunoassay System Sample Processing Online Training course. Listed below are the key points that have been presented. Take time to review the material before you proceed to the final quiz. Key Points: In this online training course, you learned how to:   Distinguish between the operating modes The time to result is shorter in turbo mode. All of the sample processing and maintenance procedures are the same for both modes. Different kits are required for each mode. Standard and turbo reagents cannot be onboard at the same time. The Home screen is yellow in standard mode and purple in turbo mode. Identify kit components Kit Barcode - The kit barcode is located on the box flap inside the kit and must be entered the first time a kit lot is used, before the reagent wedges and test units are loaded. IFU - Contains specific information regarding the assay, including how to handle and store kit components. Important Notice - Contains information regarding usage of the assay kit components. Reagent Wedge - Should not be stored onboard the reagent carousel.  After being opened, the stability is 30 days. Test Units - Contain assay-specific beads, which are the solid phase of the reaction. Each bag contains 25 test units and a desiccant. Adjustors - Each kit contains one or two adjustors. Kits with two adjustors have a low and a high adjustor. The adjustor can be in either liquid or lyophilized form. List the steps to process patient samples Sample cups can be identified on the Worklist Entry screen until the test unit is read at the PMT Sample cups are loaded on the load chain followed by a maximum of five test units. Sample status can be monitored on the Home screen and Worklist Display screen.   List the steps to process onboard dilutions Onboard dilutions are required when running controls and patient samples for some of the infectious disease assays. The only assay that has onboard dilutions as optional when running patient samples is HCG. When running an onboard dilution, the appropriate diluent wedge and reagent wedge for that test must be loaded on the reagent carousel before loading the sample cups and test units. The dilution sequence must be used to load the samples. The dilution sequence is sample cup with sample followed by an empty dilution cup followed by a space and then a maximum of four test units.   Navigate the Review screen  The results review function is used to view results from a sample previously run. To access the Review screen, select Review from the horizontal toolbar. A blank Review screen displays.  Results will appear on the screen when a time frame is selected. Select Next to continue.     The IMMULITE 1000 system has two operating modes: standard and turbo. The time to result is shorter in turbo mode. Different kits are required for each mode.  Standard and turbo reagents cannot be onboard at the same time. All of the sample processing and maintenance procedures are the same for both modes. Select each of the tabs below to learn about the different operating modes. When complete, select Next to continue. Select the Run IMMULITE button on Startup screen when initializing software. Time to first result is 40 minutes for a single cycle assay. The main incubation carousel steps one position per cycle and shakes 3x per cycle. The luminometer steps one position per cycle. The Home screen color is yellow.   Select the Run Turbo button on Startup screen when initializing software. Time to first result is 15 minutes for a single cycle assay. The main incubation carousel steps five positions per cycle and performs 3 x 15 oscillations per cycle. The luminometer steps three positions per cycle. The Home screen color is purple. The components that make up an IMMULITE 1000 system kit include the following:  IFU (Instructions for Use) Kit Barcode Test Units Reagent Wedge Adjustors  NOTE:  Some kits will also have kit-specific controls included in the kit (for example, Insulin and some infectious disease kits) Kit Components Learn about kit components. Base ImageHotspotsText BlocksImage File Test Units  Test units contain assay-specific beads, which are the solid phase of the reaction. The test unit serves as a reaction vessel in which the immune reaction, incubation, wash, and signal development processes occur. Each bag contains 25 test units and a desiccant.    Kit Barcodes The kit barcodes are located on the box flap inside the kit. Information specific to the kit lot is included in the barcode.  The kit barcodes must be entered the first time a kit lot is used before any of the new kit components are loaded on the system.    Reagent Wedge  A barcoded reagent wedge contains an assay-specific enzyme conjugate. During test processing, the reagent is pipetted into the test unit. Reagent wedges are not intended to be stored onboard the reagent carousel.  The reagent carousel is cooled, but not at refrigerator temperature.  Reagent wedges can stay onboard for up to 8 hours while they are used, but should be capped and returned to the refrigerator when the daily work is finished. The stability of reagent wedges is 30 days after they are opened.     Adjustors  Each kit contains one or two adjustors. Kits with two adjustors have a low and a high adjustor, which contain different concentrations of the analyte. The adjustors can be in either liquid or lyophilized form.   IFU  The Instructions for Use contain specific information regarding the assay, including how to handle and store kit components. It is good practice to read the IFU before using a new kit.   Kit Barcode Scanning Learn how to scan kit barcodes. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90005450/sim_IM1_ScanKitBarcodes__9/sim_IM1_ScanKitBarcodes__9.swfHTML5 File:/content/generator/Course_90005450/sim_IM1_ScanKitBarcodes__9/index.htmlPDF File: Reagent Loading Learn how to load reagent wedges.   Select the play arrow to begin video.    When complete, select the X in the upper-right-hand corner to close the window and continue.     Atrial fibrillation is the most common type of arrhythmia, its incidence increases with age. Atrial fibrillation leads to a loss of organized atrial contraction and to asynchronous atrial and ventricular activity. It can result in a reduction in cardiac output at rest by up to 20%. The effect may be even more pronounced if other cardiac diseases are present.*   Processing patient samples on the IMMULITE 1000 system involves the following steps: Identify the sample cup on the Worklist Entry screen Load the sample cups and test units on the load chain Monitor the sample status in the software NOTE: Patient information can be identified in the Worklist Entry screen at any time before the test units are read at the PMT. Patient Sample Identification Learn how to identify patient samples. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90005450/sim_IM1_IdentifyPatientSamples_9/sim_IM1_IdentifyPatientSamples_9.swfHTML5 File:/content/generator/Course_90005450/sim_IM1_IdentifyPatientSamples_9/index.htmlPDF File: Patient Loading Learn how to load patients. To load the patient samples, transfer the patient sample to the appropriate sample cups according to the information that was programmed into the Worklist Entry screen for the patients.  The sample volume required varies with the assay to be run and the number of replicates requested on that sample.  The exact sample volume required for each test can be found in the corresponding instructions for use. For proper instrument operation, an additional dead volume is required, which is based on sample volume: Sample Volume Dead Volume Less than 100 µl 100 µl Greater than or equal to 100 µl 250 µl After preparing the patient sample cups, place each sample cup on the load chain followed by test unit(s) for each test that needs to be run for that patient.  Up to five test units can be placed immediately behind each sample cup. When complete, select the X in the upper-right corner to close the window and continue.   Sample Status Learn how to monitor sample status. Tab TitleTextHome Screen  The Home screen provides the operator with an overview of the system’s status. Colored circles on the main incubation carousel represent sample cups and test units that were read by the barcode reader.  Sample cups display as yellow circles. Test units display in the assay color.  The assay color can also be seen in the circles around the reagent circle on the Home screen. A white ring around a test unit circle indicates multiple incubation cycles are required. White circles indicate an error condition for that sample cup or test unit.    Worklist Display Screen The Worklist Display screen is accessed by selecting the Worklist button on the Worklist Display screen.  This screen displays information for all the records entered in the Worklist Entry screen: Status Type of sample Tests run on the sample When complete, select the X in the upper-right corner to close the window and continue.   PVA has become an established method of treating atrial fibrillation. Primary success in over 90% of patients is reported, and 80% of patients are still in sinus rhythm after 12 months. However, ongoing antiarrhythmic drug therapy is still required.*   The IMMULITE 1000 system is capable of performing onboard dilutions for certain assays. Supplies needed for onboard dilutions include: Diluent wedge Dilution cup holder Note: Refer to the kit IFU to determine if an assay can be diluted onboard. Onboard Dilutions Learn about onboard dilutions. Slide NumberText BlocksCalloutsAudio ScriptImage File1 Onboard Dilutions Onboard dilutions are required when running controls and patient samples for some of the infectious disease assays, including: Toxoplasma Rubella H. Pylori Anti-HBc SHBG For HCG, an onboard dilution of x41 is optional when running patient samples.  Onboard dilutions are not performed on adjustors. Select Next to continue. Note: If audio does not automatically start, select the play arrow in the top left to begin.Onboard dilutions are required when running controls and patient samples for some of the infectious disease assays. The only assay that has onboard dilutions as optional when running patient samples is HCG. The dilution factor available for onboard HCG dilutions is 41. Select Next to continue. 2 Diluent Wedges The diluent used for onboard dilutions is packaged in diluent wedges.  When running an onboard dilution, the appropriate diluent wedge and reagent wedge for that test must be loaded on the reagent carousel before loading the sample cups and test units. Refer to the Kit Components section of this training course for information on how to load wedges on the reagent carousel. Select Next to continue. The diluent used for onboard dilutions is packaged in diluent wedges. When running an onboard dilution, the appropriate diluent wedge and reagent wedge for that test must be loaded on the reagent carousel before loading the sample cups and test units. Diluent wedges are loaded in the reagent carousel using the same procedure that is used to load reagent wedges. Select next to continue.3 Dilution Sequence When the system is processing an onboard dilution, the dilution is prepared in a dilution cup.  Dilution cup holders are similar to sample cup holders, except they are labeled as dilution cups with a yellow band. Onboard dilutions must be loaded on the load chain in the following sequence: Sample cup holder containing a sample cup with the sample to be diluted Dilution cup holder with an empty sample cup inside Empty space after the dilution cup holder Maximum of four test units behind the dilution cup holder NOTE:  All tests following a dilution cup holder must require the same dilution factor and diluent. Select Next to continue. When the system is processing an onboard dilution, the dilution is prepared in a dilution cup. Dilution cup holders are similar to sample cup holders, except they are labeled as dilution cups with a yellow band. Onboard dilutions must be loaded on the load chain in the dilution sequence. First, a sample cup holder containing a sample cup with the sample to be diluted is loaded. Next, a dilution cup holder with an empty sample cup is placed behind the sample cup holder. An empty space must be left on the load chain after the dilution cup holder, and then a maximum of four test units can be loaded for the dilution. All tests following a dilution cup holder must require the same dilution factor and diluent. Select Next to continue. 4 Summary To perform an onboard dilution: The appropriate diluent wedge and reagent wedge must be loaded on the reagent carousel. The dilution sequence must be used to load the samples:  sample cup with sample, empty dilution cup, space, maximum of four test units   When complete, select the X in the upper-right corner to close the window and continue.In summary, to perform an onboard dilution the appropriate diluent wedge and reagent wedge must be loaded on the reagent carousel and the dilution sequence must be used to load the samples. The dilution sequence is sample cup with sample followed by an empty dilution cup followed by a space and then a maximum of four test units.