IMMULITE® 2000 Immunoassay System Allergy Online Training

The system water bottle can be filled at any time, including while the system processing samples.  If the system is directly plumbed to a water source, the water bottle does not need to be filled.  Select the link to learn how to fill the water bottle. Welcome to the IMMULITE® 2000 System Allergy Online Training course. This course introduces you to: Loading allergy components on the system Running allergy adjustors, controls, and patients Select Next to continue. Identify and load the allergy kit components Recall the unique characteristics of allergy assays Run allergy adjustors, controls, and patients Upon successful completion of this course, you will be able to: Select Next to continue. The sample tip waste bin can be emptied at any time, including while the system processes samples. Sample tips are collected in a reservoir while the bin is removed from the system. The reservoir holds enough tips for approximately 5 minutes of operation.  Select the link to learn how to empty the sample tip waste. Congratulations you have completed the IMMULITE 2000 System Allergy online training course. Listed below are the key points that have been presented. Take time to review the material before you proceed to the final quiz.   In this online training course, you learned how to: 4 Recall the unique characteristics of allergy assays.  Allergy assays measure the amount of immunoglobulin to a specific allergen in a patient's serum.  These assays are different from other assays in that the beads are not specific to any particular allergen immunoglobulin. Identify and load the following allergy kit components: Kit IFU and Control IFU - The Kit IFU(Instructions for Use) contains specific assay information, including how to handle and store kit components. The Control IFU contains specific information regarding control information, including how to handle and store controls. Universal Reagent Wedge - Reagent wedges can remain on the system until they are empty or expired.  The onboard stability of reagent wedges is 90 days. Universal Bead Pack - Bead packs can remain on the system until they are empty or expired. The onboard stability of bead packs is 90 days. Allergens for Adjustors and Controls - Allergy kits include adjustor allergens for running adjustors and control allergens for running controls.  Adjustor and/or control allergens must be loaded in an allergen wedge and placed on the system when adjustors and/or controls for an allergy kit are being run. The 2D allergen barcode must be scanned before a new lot of allergen is loaded into an allergen wedge. Controls - Each kit contains two levels of controls, level 1 and 2.  These controls monitor the integrity of the universal kit. Each kit also contains control barcode labels, which are used to identify the sample tube as a control. Adjustors - Each kit contains two adjustors, a low and a high adjustor.  These adjustors contain different concentrations of IgE. The adjustors are in liquid form. Each kit also contains adjustor barcode labels, which are used to identify the sample tube as an adjustor. Identify the following additional allergy components: Allergen Wedge - An allergen wedge can hold up to six allergen vials.  Information about the contents of an allergen wedge is entered into the database when the wedge and vial barcodes are scanned with the hand-held 2D barcode scanner. Patient Allergens - These vials must be placed in an allergen wedge on the reagent carousel.  Allergen vials contain 20 or 40 tests, based on the allergen type.  Each allergen vial has a 2D barcode that must be scanned like the 2D barcodes from an assay kit.  Septums and Caps - When placing an allergen vial in an allergen wedge, the standard cap on each allergen vial must be replaced with a septum cap.  A septum cap is a latex rubber cap that is slit to allow the reagent probe to enter the vial without damage and to minimize evaporation. When storing opened allergen vials off the instrument, replace the septum cap with a new standard cap to prevent evaporation. Load a universal bead pack: Place the bead pack in a carousel slot with the barcode facing out Tilt down the side of the bead pack opposite to the barcode label Snap down the barcoded side of the bead pack to lock it in place Close the bead carousel cover Prepare a universal reagent wedge for loading: Remove the clear tape and foil seal from the wedge Check the wedge for bubbles and ensure that the top of the wedge and the glide are dry Ensure the glide moves back and forth smoothly on top of the wedge Load a universal reagent wedge: Tilt the barcode side of the wedge down as the wedge is placed in a carousel slot Press down on the narrow side of the wedge to lock it in place Ensure the glide moves freely Close the reagent carousel cover Load an allergen wedge: Scan allergen vial barcode flag Place septum cap on allergen vial Load vials into allergen wedge Scan allergen wedge barcode. Load allergen wedge onto reagent carousel Run allergy adjustors: Load adjustor allergens on the system Load adjustor sample tubes on the system and put the system into Run mode Select Worklist Select Next Enter kit lot number and adjustor allergen lot number Select Accept Adjustor Review Display / Edit screen Run allergy controls: Load control allergens on the system Load allergy control samples on the system and put the system into Run mode Select Worklist Select Next Order tests Select Accept Control Review Display/Edit screen To run allergy patients, load patient samples on the system and put the system into Run mode.  After the barcodes are read, the system will automatically start running any test orders that are sent from the LIS.  If the LIS is down or if a barcode label is damaged, you can still process allergy patient samples manually.  Refer to the sample processing competency for information on manually ordering patient samples.    Select Next to continue. Let's review the proper storage requirements and mixing techniques for primary reagents. Reagents should be protected from light and stored upright in the refrigerator. During storage the particles in the Solid Phase reagent settle at the bottom of the primary reagent pack.  These particles  must be resuspended homogeneously before use to provide maximum reagent and assay efficiency.  Mix all primary reagent packs by hand before loading them on to the system.  Following the proper preparation procedure, reagents are loaded on to the instrument.  The primary reagent pack is loaded into the primary reagent compartment. If you remove the reagents, cover the pierced film area of the primary reagent pack with self-sealing laboratory film and place the pack in the refrigerator.   If the reagents are not stored and mixed properly, calibration, QC and patient results may be affected. Ancillary reagent packs should be gently inverted several times. Ancillary reagents are loaded onto the ancillary reagent entry area.  If you remove reagents, cover the pierced area of the ancillary pack with self-sealing laboratory film and store in the refrigerator.  To mix a pierced pack, cover the pierced film area and gently invert the pack several times. Select the links below to learn about mixing and loading reagent packs. Allergy assays measure the amount of immunoglobulin (IgE) to a specific allergen in a patient's serum. These assays are different from other assays in that the beads are not specific to any particular allergen immunoglobulin. Allergy Assays Learn more about Allergy Assays.   Select the play arrow to begin the video. When complete, select the X in the upper-right corner to close the window and continue. Let's look at the components that make up a 3gAllergen Specific IgE (SPE) kit. Components found inside the allergy kit box: Kit IFU and Control IFU Universal Reagent Wedge Universal Bead Pack Allergens for Adjustors and Controls Controls Adjustors Additional allergy components: Allergen Wedge Patient Allergens Septums and Caps 3gAllergen Sepcific IgE (SPE) Kit Components Learn about 3gAllergen Specific IgE (SPE) kit components. Base ImageHotspotsText BlocksImage File Kit IFU and Control IFU The Kit IFU (Instructions for Use) contains specific assay information, including how to handle and store kit components. The Control IFU contains specific information regarding control information, including how to handle and store controls. It is good practice to read the IFUs before using a new kit and/or controls. Allergy Kit Barcode The kit barcode is located on the box flap inside the kit.  Information specific to the kit lot is included in the barcode and must be entered the first time a kit lot is used. Universal Reagent Wedge A barcoded universal reagent wedge contains an anti-immunoglobulin reagent used for all allergy tests with all allergens.  During test processing, the reagent is pipetted into the reaction tube. Reagent wedges can remain on the system until they are empty or expired.  The onboard stability of reagent wedges is 90 days. Universal Bead Pack A barcoded universal bead pack contains streptavidin-coated beads, which are the solid phase of the reaction.  During test processing, a single bead is dropped into a reaction tube. Bead packs can remain on the system until they are empty or expired.  The onboard stability of bead packs is 90 days. Adjustor and Control Allergens Allergy kits include adjustor allergens for running adjustors and control allergens for running controls.  Adjustor and/or control allergens must be loaded in an allergen wedge and placed on the system when adjustors and/or controls for an allergy kit are being run.  Each adjustor and control allergen vial contains 40 tests and has a corresponding 2D barcode that contains lot-specific information about the allergen.  The 2D barcode must be scanned before a new lot of allergen is loaded into an allergen wedge. Universal Allergy Control Bottles and Barcodes  Each kit contains two levels of controls, level 1 and level 2.  These controls monitor the integrity of the universal kit. Each kit also contains control barcode labels, which are used to identify the sample tube as a control. Universal Allergy Adjustor Bottles and Barcodes  Each kit contains two adjustors, a low and a high adjustor.  These adjustors contain different concentrations of IgE.  The adjustors are in liquid form.  Each kit also contains adjustor barcode labels, which are used to identify the sample tube as an adjustor. Additional Allergy Components Learn about additional allergy components. Tab TitleTextAllergen Wedge Allergen wedges are the wedge-shaped frames that hold the allergen vials used in allergy testing.  An allergen wedge can hold up to six allergen vials.   Information about the contents of an allergen wedge is entered into the database when the wedge and vial barcodes are scanned with the hand-held 2D barcode scanner.    Allergen wedges also have a barcode on the edge, like a reagent wedge, so their position on the reagent carousel can be identified by the sample/reagent barcode reader.    Patient Allergens Patient allergens are vials containing a specific biotinylated allergen to test patient samples for a particular allergen.  These vials must be placed in an allergen wedge on the reagent carousel.  Vials that contain several allergens are used to test samples for a broader range of allergic reactions.  Allergen vials contain 20 or 40 tests, based on the allergen type.  Each allergen vial has a 2D barcode that must be scanned like the 2D barcodes from an assay kit.    Septums and Caps When you place an allergen vial in an allergen wedge, the standard cap on each allergen vial must be replaced with a septum cap.  A septum cap is a latex rubber cap that is slit to allow the reagent probe to enter the vial without damage and to minimize evaporation. When you store opened allergen vials off the instrument, replace the septum cap with a new standard cap to prevent evaporation. When complete, select the X in the upper-right corner to close the window and continue.   To run allergy tests, the following components must be loaded onto the system: Universal Bead Pack Universal Reagent Wedge Allergen Wedge Loading Universal Bead Packs Learn more about loading universal bead packs.   Select the play arrow to begin video.  When complete, select the X in the upper-right corner to close the window and continue. Loading a Universal Reagent Wedge Learn more about loading a universal reagent wedge.   Select the play arrow to begin the video.  When complete, select the X in the upper-right corner to close the window and continue. Loading an Allergen Wedge Learn how to load an allergen wedge. Checklist TitleChecklist TypeChecklist ContentScan allergen vial barcodeHTML   Select the play arrow to begin the video.   Select each checkbox to learn more about loading the allergen wedge. Place septum cap on allergen vialHTML   Select the play arrow to begin the video.   Load vials into allergen wedgeHTML   Select the play arrow to begin the video.   Scan allergen wedge barcodeHTML   Select the play arrow to begin the video.   Load allergen wedge onto reagent carouselHTML   Select the play arrow to begin the video.   When complete, select the X in the upper-right corner to close the window and continue. Congratulations.  You have completed the ADVIA Centaur XPT System Sample Processing Online Training course.  Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz. Like all of the other IMMULITE 2000 System assays, the allergy assays must be adjusted before running controls and patient samples. Let's look at how to run allergy adjustors.   Running Allergy Adjustors Learn how to run allergy adjustors. Checklist TitleChecklist TypeChecklist ContentLoading adjustor allergensHTML   Select the play arrow to begin the video.   Select each checkbox to learn more about running allergy adjustors. Loading adjustor sample tubesHTML    Select the play arrow to begin the video. Running Allergy AdjustorsSimulationInstructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90004001/sim_IMM2K_RunningAllergyAdjustors_9/sim_IMM2K_RunningAllergyAdjustors_9.swfHTML5 File:/content/generator/Course_90004001/sim_IMM2K_RunningAllergyAdjustors_9/index.htmlPDF File: There are two types of controls for allergy assays: Specific IgE (SPE) Kit controls, which monitor the integrity of the universal kit Allergen specific controls, which monitor the integrity of specific allergens Let's look at how to run allergy controls. Running Allergy Controls Learn about how to run allergy controls. Checklist TitleChecklist TypeChecklist ContentLoading control allergensHTML   Select the play arrow to begin the video.   Select each checkbox to learn more about running allergy controls. Loading control sample tubesHTML   Select the play arrow to begin the video.   Running Allergy ControlsSimulationInstructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90004001/sim_IMM2K_RunningAllergyControls_9/sim_IMM2K_RunningAllergyControls_9.swfHTML5 File:/content/generator/Course_90004001/sim_IMM2K_RunningAllergyControls_9/index.htmlPDF File: If the system is in Run mode, the IMMULITE 2000 System will automatically start running barcoded allergy patient samples with LIS orders. If the LIS is down or if a barcode label is damaged, you can still process allergy patient samples manually.  Refer to the Sample Processing competency for information on manually ordering allergy patient samples.   Loading Barcoded Allergy Patient Samples Learn more about loading barcoded allergy patient samples.   Select the play arrow to begin the video.  When complete, select the X in the upper-right corner to close the window and continue.