IMMULITE® 2000 Immunoassay System Overview Online Training

Welcome to the IMMULITE® 2000 System Overview Online Training course. This course introduces you to: The location and function of the main components of the system How to navigate through the system software Select Next to continue. Identify and locate consumables Locate system components and identify the function of each component Navigate the Home Screen, Bead and Reagent Screens, Help Screen, and the LIS Screen Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations.  You have completed the IMMULITE 2000 System Overview Online Training course. Listed below are the key points that have been presented. Take time to review the material before you proceed to the final quiz. Key Points: In this online training course, you learned how to: Locate and identify the function of the following top cover parts:     Reagent Carousel Cover - Opening the reagent carousel cover allows you to load and unload reagent wedges                       Bead Carousel Cover - Opening the bead carousel cover allows you to load and unload bead packs Sample Carousel Cover -  Opening the sample carousel door allows you to load and unload sample racks                              DRD Priming / Substrate Reservoir Door - Can be used to view the DRDs during priming and to load substrate onto the system                                       Monitor Arm - Includes the touch screen monitor, keyboard, mouse, and hand-held 2D barcode scanner                   Locate and identify the function of the following interior parts: Sample Carousel - Holds up to six racks with up to 15 tubes of varying sizes per rack Reagent Carousel - Holds up to 24 reagent wedges and is refrigerated Pipettors - Dispense sample and reagent Sample Dilution Well - Mixes specified quantities of specimen, diluent, and water DRDs - Pumps that move sample and reagent Water Pump - Dispenses water into a reaction tube at the bead/tube wash station Substrate Pump - Dispenses 200 µL of substrate into a reaction tube Bead Carousel - Holds up to 24 bead packs and is dehumidified Tube Processor - Includes the Incubator, Bead/Tube Wash Station, Luminometer, and PMT Locate and identify the function of the following consumables (supplies needed to run tests on the system): Substrate Reservoir - Holds up to 1000 tests worth of chemiluminescent substrate Water Bottle - Holds up to 6 liters of distilled or deionized water Probe Wash Bottle - Holds up to 2 liters of prepared probe wash that is used to wash the sample and reagent pipettors Solid Waste Container - Collects the used reaction tubes after they are read by the PMT Liquid Waste Bottle - Collects waste fluids from the pipettor wash wells, dilution well, and the bead/tube wash station Tube Hopper - Holds up to 1300 reaction tubes Navigate the following main software screens: Home Screen - Displays a representation of the sample, reagent, and bead carousels Bead and Reagent Screens -  Used to check the status of the reagent wedges, allergen wedges, and bead packs onboard the system Help Screen -  Provides information about the operation of the system and its software LIS Screen - Used to send results to the LIS manually Select Next to continue.          The IMMULITE 2000 System top cover parts include the following: Reagent Carousel Cover                      Bead Carousel Cover Sample Carousel Door                                        DRD Priming / Substrate Reservoir Door                                        Monitor Arm                                                                                                                                                                                     Top Cover Parts Learn about the top cover parts. Base ImageHotspotsText BlocksImage File Bead Carousel Cover:  Opening the bead carousel cover allows you to load and unload bead packs.  If this door is opened while the system is in Run mode, the system will automatically be put into bead pause mode.  In this mode, the bead carousel is paused but tests that have already been pipetted will continue processing.   Reagent Carousel Cover :  Opening the reagent carousel cover allows you to load and unload reagent wedges.  If this door is opened while the system is in Run mode, the system will automatically be put into reagent pause mode.  In this mode, the reagent carousel is paused but tests that have already been pipetted will continue processing.    DRD Priming / Substrate Reservoir Door:  The DRD priming / substrate reservoir door allows you to view the DRDs during priming and to load substrate onto the system.  If this door is opened while the system is in Run mode, the system will NOT be put into a pause mode.   Monitor Arm (Monitor, Keyboard, Mouse, Hand-held 2D Barcode Scanner):  The monitor arm includes the touch screen monitor, keyboard, mouse, and hand-held 2D Barcode Scanner.  Sample Carousel Door:  Opening the sample carousel door allows you to load and unload sample racks.  If this door is opened while the system is in Run mode, the system will automatically be put into sample pause mode.  In this mode, the sample carousel is paused but tests that have already been pipetted will continue processing.   The IMMULITE 2000 System interior parts include the following: Sample Carousel                                          Reagent Carousel Pipettors Dilution Well DRDs (Dual Resolution Dilutors) Pumps                                                Bead Carousel Tube Processor   Parts Inside the System Learn about the parts inside the system. Slide NumberText BlocksCalloutsAudio ScriptImage File1Sample Carousel Holds up to six racks Holds up to 15 tubes of varying sizes per rack Barcoded and unbarcoded tubes can be used     Select Next to continue.                          Note: If audio does not automatically start, select the play arrow in the top left to begin.The sample carousel holds six removable racks. Each rack holds up to 15 specimen or diluent tubes of varying sizes. Both barcoded and unbarcoded tubes can be run on the sample carousel. Select Next to continue.2 Reagent Carousel Holds up to 24 reagent wedges Refrigerated Reagent wedges can be stored onboard   Select Next to continue. The reagent carousel holds 24 reagent wedges or allergen wedges. Since the reagent carousel is refrigerated, reagent wedges can be stored on the system. Select Next to continue.3 Pipettors / Dilution Well   Reagent Pipettor    Dispenses reagent     Sample Pipettor Dispenses sample Clot detection transducer     Sample Dilution Well     Mixes specified quantities of specimen, diluent, and water The sample probe dispenses the materials into the well and then transfers the diluted sample to a reaction tube         Select Next to continue. Select each number to review the corresponding text.CalloutsSample PipettorSample Dilution WellReagent Pipettor The reagent pipettor dispenses reagent onto the bead in the reaction tube, while the sample pipettor dispenses sample also onto the bead in the reaction tube. The sample probe has a clot detection transducer, which allows for an error message to be generated when a clot is detected in a sample tube. The sample dilution well mixes specified quantities of specimen, diluent, and water to form a homogenous mixture. The sample probe dispenses the materials into the well for mixing. After the diluted sample is pipetted into the reaction tube, the dilution well insert is spun at a high speed, discarding the unused portion of the diluted sample. Select Next to continue. 4 DRDs (Dual Resolution Dilutors) / Pumps   DRDs The DRDs are pumps that move sample and reagent Pumps The water pump dispenses water into a reaction tube at the bead/tube wash station The substrate pump dispenses 200 µL of substrate into a reaction tube Select Next to continue. Select each number to review the corresponding text.CalloutsSample DRDReagent DRDSubstrate PumpWater PumpThe sample and reagent DRDs are pumps that move sample and reagent to the pipettors. DRD stands for dual resolution dilutor. The water pump accurately dispenses water into a reaction tube at the bead/tube wash station. The substrate pump dispenses 200 µL of substrate from the substrate reservoir into a reaction tube. Select Next to continue.5 Bead Carousel Holds up to 24 bead packs Dehumidified chamber Bead packs can be stored onboard     Select Next to continue. The bead carousel is a dehumidified carousel that holds 24 bead packs. Select Next to continue.6Tube Processor The tube processor consists of the incubator, wash station, luminometer and PMT. Incubator Immune and luminogenic reactions incubate Reaction tubes continually agitate at 37°C Bead/Tube Wash Station Washes and spins the bead after immune reaction Substrate Probe Substrate probe adds substrate to the reaction tube  Luminometer Tubes incubate after the addition of substrate at 37°C  Chemiluminescent reaction occurs    PMT (Photomultiplier Tube) Measures the photon counts of the chemiluminescent reaction Select Next to continue.       Select each number to review the corresponding text.CalloutsSubstrate ProbeIncubatorLuminometerPMT (Photomultiplier Tube)Bead/Tube Wash StationThe tube processor consists of the incubator, wash station, luminometer and PMT. The incubator is the device where the immune and luminogenic reactions take place. Reaction tubes are continually agitated at 37°C during these processes. The bead/tube wash station washes and spins the bead after the immune reaction to remove any residual unbound reagent and sample, and then the substrate probe adds substrate to the reaction tube. After the addition of substrate, the reaction tubes are incubated in the luminometer, where the chemiluminescent reaction occurs. The PMT, or photomultiplier tube, measures the photon counts. Select Next to continue.7 Wrap-Up You have just learned about the function of each of the system interior parts: Sample Carousel                                            Reagent Carousel Pipettors Dilution Well DRDs Pumps                                                  Bead Carousel Tube Processor     When complete, select the X in the upper-right window and continue.Select each number to review the corresponding text.CalloutsSample CarouselReagent CarouselPipettorsDilution WellDRDsPumpsBead CarouselTube ProcessorYou have just learned about the function of each of the system interior parts. The IMMULITE 2000 System consumables are supplies needed to run tests on the system.  They include the following: Substrate Reservoir Water Bottle Probe Wash Bottle Solid Waste Container Liquid Waste Container Tube Hopper Consumables Learn about consumables. Base ImageHotspotsText BlocksImage File Substrate Reservoir:  The substrate reservoir holds enough chemiluminescent substrate to process up to 1000 tests.   Water Bottle:  The water bottle holds up to 6 liters of distilled or deionized water.  Probe Wash Bottle:  The probe wash bottle holds up to 2 liters of prepared probe wash that is used to wash the sample and reagent pipettors.  Solid Waste Container:  The solid waste container collects the used reaction tubes after they are read by the PMT.  Liquid Waste Bottle:  The liquid waste bottle collects waste fluids from the pipettor wash wells, dilution well, and the bead/tube wash station.  Tube Hopper:  The tube hopper holds up to 1300 reaction tubes.  The reaction tubes are vessels in which sample, reagent, bead and substrate are brought together for analysis. Now let's take a look at the most commonly used software screens on the IMMULITE 2000 System. They include the following: Home screen Bead/Reagent screen Help screen LIS screen Select Next to continue.   The IMMULITE 2000 System Home screen displays the following: Horizontal Toolbar Vertical Toolbar Consumables Indicators Sample Racks Reagent Carousel Bead Carousel Results View Home Screen Learn about the Home screen. Base ImageHotspotsText BlocksImage File Horizontal Toolbar:  The horizontal toolbar provides quick access to commands or screens used in routine instrument operation as well as tools for data management.          Vertical Toolbar:  The vertical toolbar directly affects instrument operations.  The top three buttons are used to put the system into RUN, PAUSE, or STOP mode.  The PRIME button can be used to prime the DRDs and the COVER button is used to unlock the top cover of the system.   Consumables Indicators:  The consumables indicators display the instrument fill-levels for water, probe wash, substrate, the tube hopper, and liquid and solid waste.  Sample Racks:  The sample rack icons display the sample racks that are onboard.  Letters on the sample rack icons identify the rack positions on the sample carousel.  Selecting a sample rack icon opens the sample rack detail screen, which lists the accession numbers for the tubes in that sample rack. Reagent Carousel:  Selecting the Reagents icon opens up the Reagent screen, which displays onboard reagents and their status.  Bead Carousel:   Selecting the Beads icon opens up the Bead screen, which displays onboard beads and their status.      Find Button:  The FIND button offers sample search capabilities.  You can search by accession number, name, and test type for a tube's location on the system.  Results View:  The window in the lower right hand side of the screen will display the most recent test results.   RAPIDComm windows consist of a menu bar, toolbar, left pane, right pane, status bar and help buttons.  Select the link on this page to learn about these features. The IMMULITE 2000 System Bead and Reagent screens are accessed by selecting the bead pack and reagent wedge icons on the Home screen.  The Bead and Reagent screens are used to check the status of the following: Reagent wedges Allergen wedges Bead packs    Bead / Reagent Screen Learn about the Bead and Reagent screens. Slide NumberText BlocksCalloutsAudio ScriptImage File1 Overview of Reagent Screen The Reagent Status screen displays the status of all allergen wedges and reagent wedges on the reagent carousel. The information displayed in the Reagent Status screen includes:   Reagent wedge test codes or allergen wedge IDs Allergen vial status for each allergen wedge Reagent wedge lot numbers Error messages Adjustment status Number of tests remaining in each compartment of a reagent wedge Wedge positions on the reagent carousel      Select Next to continue.    Note: If audio does not automatically start, select the play arrow in the top left to begin.The Reagent Status screen displays the status of all allergen wedges and reagent wedges on the reagent carousel. Reagent wedges are displayed alphanumerically by test code, not by their positions on the reagent carousel. Each reagent wedge is represented by a square on the Reagent Status screen. Select Next to continue.2Overview of Bead Screen The Bead Status screen displays the status of all bead packs on the bead carousel. The information displayed in the Bead Status screen includes: Bead pack names Bead pack lot numbers Error messages Number of tests remaining in bead packs Bead pack positions on the bead carousel    Select Next to continue.    The Bead Status screen displays the status of all bead packs on the bead carousel. Bead packs are displayed alphanumerically by test code, not by their positions on the bead carousel. Each bead pack on the bead carousel is represented by a square in the Bead Status screen. Select Next to continue. 3Background Colors On the Reagent and Bead Status screens, the status of each position is indicated by the color of its square: White= No errors Gray = Empty position Light Brown = Warning Red = Error Condition  Select Next to continue.  On the Reagent and Bead Status screens, the status of each position is indicated by the color of its square. White indicates no errors, gray indicates an empty position, light brown indicates a warning such as low tests or adjustment due, and red indicates an error condition such as never adjusted or not in database. Select Next to continue. 4Viewing Details Details for reagent wedges and bead packs can be viewed in the Reagent Detail or Bead Detail screen. The information in these screens include:  The lot numbers for that kit Expiration and Use by dates Adjustment information A list of errors that have occurred for that reagent wedge or bead pack Select Next to continue. Select the number to review the corresponding text.  Callouts Review Button When the system is in Pause or Stop mode, select the Rotate button at the top of the screen to open the Reagent or Bead detail screen. The button changes to the Review button. Then, select the square corresponding to the reagent wedge or bead pack to be viewed. The Reagent or Bead Detail screen displays. To display the Reagent or Bead Detail screens while the instrument is in Run mode, select the square for the reagent wedge or bead pack in the Reagent or Bead Status screen. Select Next to continue.5Wrap-Up You have just learned about the Bead and Reagent screens. These screens are useful for viewing information about the onboard bead packs and reagent wedges, including: Adjustment status Number of tests left Lot numbers Error messages When complete, select the X in the upper-right window and continue.You have just learned about the Bead and Reagent screens. These screens are useful for viewing information about the onboard bead packs and reagent wedges, including adjustment status, number of tests left, lot numbers, and error messages. Welcome to the RAPIDComm® Data Management System Daily Operations Online Training course. In this course, you will learn how to perform daily tasks using the system software. Select Next to continue. The IMMULITE 2000 System LIS screen is accessed by selecting the LIS button on the horizontal toolbar.  If the system is not configured to send results automatically, the LIS screen can be used to send results to the LIS manually.  Also, the LIS screen can be used to view and search for results.     LIS Screen Learn about the LIS screen. Base ImageHotspotsText BlocksImage File Auto Send and Re-Send Buttons:  The Auto Send and Re-Send buttons at the top of the screen are used to send results to the LIS.  The Re-Send button is used if a result already has a sent status.  Note that the Auto Send button will change to a Send button if the system is not configured to automatically send results to the LIS.    LIS Worklist Area:  In the main area of the LIS screen, a list of pending orders and results is displayed.  Results are selected to be sent to the LIS or cleared by tagging them.  Individual results can be tagged by selecting them on the screen, or all the results can be tagged by selecting the Tag All button.  Sort By Button:  The Sort By button is used to select a date range for the data.  This option also determines the order that the records are sorted by. LIS Active Button:   If the LIS is not communicating with the IMMULITE 2000 System, you may want to pause communications while troubleshooting the problem.  Selecting the LIS Active button toggles the button to LIS Paused.  This temporarily pauses communication with the LIS.  Select the LIS Paused button again once the LIS is back in service.   The records are color-coded by status: Red = Invalid Result     Green = Tagged Result     Black = Not Tagged     Grey = Sent Result Patient results may need to be validated if the RAPIDComm program diverted that sample for manual validation.  You can manually validate patient results in the Patient Sample Validation List window. Select the links below to learn more about validating patient results and handling duplicate samples. The IMMULITE 2000 System Help screen is accessed by selecting the HELP button on the horizontal toolbar.  This screen provides information about the operation of the system and its software.   Help Screen Learn about the Help screen. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003914/sim_IMM2K_Help_9/sim_IMM2K_Help_9.swfHTML5 File:/content/generator/Course_90003914/sim_IMM2K_Help_9/index.htmlPDF File: