IMMULITE® 2000 XPi Immunoassay System Allergy Online Training

Welcome to the IMMULITE® 2000 XPi System Allergy Online Training course.  This course introduces you to: Loading allergy components on the system Running allergy adjustors, controls, and patients Select Next to continue. Identify and load the allergy kit components Recall the unique characteristics of allergy assays Run allergy adjustors, controls, and patients Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations. You have completed the IMMULITE 2000 XPi System Allergy Online Training course.  Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz.  Key Points: In this online training course, you learned how to:   Recall the unique characteristics of allergy assays.  Allergy assays measure the amount of immunoglobulin to a specific allergen in a patient's serum.  These assays are different from the other assays because the beads are not specific to any particular allergen immunoglobulin. Identify and load the allergy kit components. Components found inside the allergy kit box: Kit IFU and Control IFU - The Kit IFU (Instructions for Use) contains specific assay information, including how to handle and store kit components. The Control IFU contains specific information regarding control information, including how to handle and store controls. Universal reagent wedge - Reagent wedges can remain on the system until they are empty or expired.  The onboard stability of reagent wedges is 90 days. Universal bead pack - Bead packs can remain on the system until they are empty or expired. The onboard stability of bead packs is 90 days. Allergens for Adjustors and Controls - Allergy kits include adjustor allergens for running adjustors and control allergens for running controls.  Adjustor and/or control allergens must be loaded in an allergen wedge and placed on the system when adjustors and/or controls for an allergy kit are being run. The 2D allergen barcode must be scanned before a new lot of allergen is loaded into an allergen wedge. Controls - Each kit contains two levels of controls, level 1 and 2.  These controls monitor the integrity of the universal kit. Each kit also contains control barcode labels, which are used to identify the sample tube as a control. Adjustors - Each kit contains two adjustors, a low and a high adjustor.  These adjustors contain different concentrations of IgE. The adjustors are in liquid form. Each kit also contains adjustor barcode labels, which are used to identify the sample tube as an adjustor. Additional allergy components: Allergen wedge - An allergen wedge can hold up to six allergen vials.  Information about the contents of an allergen wedge is entered into the database when the wedge and vial barcodes are scanned with the hand-held 2D barcode scanner. Patient Allergens - These vials must be placed in an allergen wedge on the reagent carousel.  Allergen vials contain 20 or 40 tests, based on the allergen type.  Each allergen vial has a 2D barcode that must be scanned like the 2D barcodes from an assay kit. Septums and Caps - When you place an allergen vial in an allergen wedge, the standard cap on each allergen vial must be replaced with a septum cap.  A septum cap is a latex rubber cap that is slit to allow the reagent probe to enter the vial without causing damage and to minimize evaporation. When storing opened allergen vials off the instrument, replace the septum cap with a new standard cap to prevent evaporation. To load a universal bead pack: Place the bead pack in a carousel slot with the barcode facing out Tilt down the side of the bead pack opposite to the barcode label Snap down the barcoded side of the bead pack to lock it in place Close the bead carousel cover Universal Reagent Wedges: Before loading a universal reagent wedge onto the system: Remove the clear tape and foil seal from the wedge Check the wedge for bubbles and ensure that the top of the wedge and the glide are dry Ensure the glide moves back and forth smoothly on top of the wedge To load a universal reagent wedge: Tilt the barcode side of the wedge down as the wedge is placed in a carousel slot Press down on the narrow side of the wedge to lock it in place Ensure the glide moves freely Close the reagent carousel cover To load an allergen wedge: Scan allergen vial barcode Place septum cap on allergen vial Load vials into allergen wedge Scan allergen wedge barcode Load allergen wedge onto reagent carousel Run allergy adjustors, controls, and patients. To run allergy adjustors: Load adjustor allergens on the system. Load adjustor sample tubes on the system. Select Worklist Select Next Enter kit lot number and adjustor allergen lot number Select Accept Adjustor Review Display/Edit screen To run allergy controls: Load control allergens on the system Load allergey control samples on the system Select Worklist Select Next Order tests Select Accept Control Review Display/Edit screen To run allergy patients: If the system is in Run mode, the IMMULITE 2000 XPi System will automatically start running barcoded allergy patient samples with LIS orders. If the LIS is down or if a barcode label is damaged, you can still process allergy patient samples manually.  Refer to the Sample Processing competency for information on manually ordering patient samples. Select Next to continue. Allergy assays measure the amount of immunoglobulin (IgE) to a specific allergen in a patient's serum. These assays are different from the other assays because the beads are not specific to a particular allergen immunoglobulin.   Allergy Assays Learn more about Allergy Assays.   Select the play arrow to begin the video. When complete, select the X in the upper-right corner to close the window and continue. Let's look at the components that make up a 3gAllergen Specific IgE (SPE) kit.   Components found inside the allergy kit box: Kit IFU and Control IFU Universal Reagent Wedge Universal Bead Pack Allergens for Adjustors and Controls Controls Adjustors Additional allergy components: Allergen Wedge Patient Allergens Septums and Caps   3gAllergen Sepcific IgE (SPE) Kit Components Learn more about 3gAllergen Sepcific IgE (SPE) kit components. Base ImageHotspotsText BlocksImage File Kit IFU and Control IFU The Kit IFU (Instructions for Use) contains specific assay information, including how to handle and store kit components. The Control IFU contains specific information regarding control information, including how to handle and store controls. It is a good practice to read the IFUs before using a new kit and/or controls. Allergy Kit Barcode The kit barcode is located on the box flap inside the kit.  Information specific to the kit lot is included in the barcode and must be entered the first time a kit lot is used. Universal Reagent Wedge A barcoded universal reagent wedge contains an anti-immunoglobulin reagent used for all allergy tests with all allergens.  During test processing, the reagent is pipetted into the reaction tube. Reagent wedges can remain on the system until they are empty, have reached the expiration date, or have exceeded the 90 day onboard stability limit.  Universal Bead Pack A barcoded universal bead pack contains streptavidin-coated beads, which are the solid phase of the reaction.  During test processing, a single bead is dropped into a reaction tube. Bead packs can remain on the system until they are empty, have reached the expiration date, or have exceeded the 90 day onboard stability limit.  Adjustor and Control Allergens Allergy kits include adjustor allergens for running adjustors and control allergens for running controls.  Adjustor and/or control allergens must be loaded in an allergen wedge and placed on the system when adjustors and/or controls for an allergy kit are being run.  Each adjustor and control allergen vial contain 40 tests and has a corresponding 2D barcode that contains lot-specific information about the allergen.  The 2D barcode must be scanned before a new lot of allergen is loaded into an allergen wedge. Allergy Control Bottles and Barcodes  Each kit contains two levels of controls, level 1 and level 2.  These controls monitor the integrity of the universal kit. Each kit also contains control barcode labels, which are used to identify the sample tube as a control. Allergy Adjustor Bottles and Barcodes  Each kit contains two adjustors, a low and a high.  These adjustors contain different concentrations of IgE.  The adjustors are in liquid form.  Each kit also contains adjustor barcode labels, which are used to identify the sample tube as an adjustor. Additional Allergy Components Learn about additional allergy components. Tab TitleTextAllergen Wedge Allergen wedges are the wedge-shaped frames that hold the allergen vials used in allergy testing.  An allergen wedge can hold up to six allergen vials.   Information about the contents of an allergen wedge is entered into the database when the wedge and vial barcodes are scanned with the hand-held 2D barcode scanner.  Allergen wedges also have a barcode on the edge, like a reagent wedge, so their position on the reagent carousel can be identified by the sample/reagent barcode reader.    Patient Allergens Patient allergens are vials containing a specific biotinylated allergen to test patient samples for a particular allergen.  These vials must be placed in an allergen wedge on the reagent carousel.  Vials that contain several allergens are used to test samples for a broader range of allergic reactions.  Allergen vials contain 20 or 40 tests, based on the allergen type.  Each allergen vial has a 2D barcode that must be scanned like the 2D barcodes from an assay kit.    Septums and Caps When you place an allergen vial in an allergen wedge, the standard cap on each allergen vial must be replaced with a septum cap.  A septum cap is a latex rubber cap that is slit to allow the reagent probe to enter the vial without causing damage and to minimize evaporation. When storing opened allergen vials off the instrument, replace the septum cap with a new standard cap to prevent evaporation. When complete, select the X in the upper-right corner to close the window and continue.   To run allergy tests, the following components must be loaded onto the system: Universal Bead Pack Universal Reagent Wedge Allergen Wedge Loading Universal Bead Packs Learn about loading universal bead packs.   Select the play arrow to begin the video. When complete, select the X in the upper-right corner to close the window and continue.   Loading a Universal Reagent Wedge Learn about loading a universal reagent wedge.   Select the play arrow to begin the video. When complete, select the X in the upper-right corner to close the window and continue.   Loading an Allergen Wedge Learn how to load an allergen wedge. Checklist TitleChecklist TypeChecklist ContentScan allergen vial barcodeHTML    Select the play arrow to begin the video. Select each checkbox to learn more about loading the allergen wedge. Place septum cap on allergen vialHTML   Select the play arrow to begin the video.   Load vials into allergen wedgeHTML     Select the play arrow to begin the video.  Scan allergen wedge barcodeHTML     Select the play arrow to begin the video.  Load allergen wedge onto reagent carouselHTML  Select the play arrow to begin the video. When complete, select the X in the upper-right corner to close the window and continue. Assay errors are errors on connected instruments that prevent the automatic completion of tests. For example, out-of-inventory errors occur when these instruments encounter a processing problem and the VersaCell X3 system does not receive detailed error information from the connected instruments. To view the details of assay errors, access either the Samples screen or the Instruments screen.  On the Samples screen, samples with assay errors display in yellow.  On the Instruments screen, assay errors may generate one or more associated Instrument Errors. Permanent order errors occur when an order exceeds an instrument’s capability, or lacks required information. Select the links to learn about resolving assay errors and permanent order errors. Like all other IMMULITE 2000 XPi System assays, the allergy assays must be adjusted before you run controls and patient samples. Let's look at how to run allergy adjustors.   Running Allergy Adjustors Learn how to run allergy adjustors. Checklist TitleChecklist TypeChecklist ContentLoading adjustor allergensHTML   Select the play arrow to begin the video. Select each checkbox to learn more about running allergy adjustors.  Loading adjustor sample tubesHTML   Select the play arrow to begin the video.  Ordering allergy adjustor testsSimulationInstructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003709/sim_IMMXPi_RunningAllergyAdjustors_9/sim_IMMXPi_RunningAllergyAdjustors_9.swfHTML5 File:/content/generator/Course_90003709/sim_IMMXPi_RunningAllergyAdjustors_9/index.htmlPDF File: There are two types of controls for allergy assays: Specific IgE (SPE) Kit controls, which monitor the integrity of the universal kit Allergen specific controls, which monitor the integrity of specific allergens Let's look at how to run allergy controls. Running Allergy Controls Learn about how to run allergy controls. Checklist TitleChecklist TypeChecklist ContentLoading control allergensHTML   Select the play arrow to begin the video. Select each checkbox to learn more about running allergy controls.  Loading control sample tubesHTML   Select the play arrow to begin the video.   Ordering allergy control testsSimulationInstructions:If media does not automatically start, select the play arrow to begin. Flash File:/content/generator/Course_90003709/sim_IMMXPi_AllergyControl_9/sim_IMMXPi_AllergyControl_9.swfHTML5 File:/content/generator/Course_90003709/sim_IMMXPi_AllergyControl_9/index.htmlPDF File: If the system is in Run mode, the IMMULITE 2000 XPi System will automatically start running barcoded allergy patient samples with LIS orders. If the LIS is down or if a barcode label is damaged, you can still process allergy patient samples manually.  Refer to the Sample Processing competency for information on manually ordering allergy patient samples.   Loading Barcoded Allergy Patient Samples Learn about loading barcoded allergy patient samples.   Select the play arrow to begin the video.  When complete, select the X in the upper-right corner to close the window and continue.