IMMULITE® 2000 XPi Immunoassay System Sample Processing Online Training

Welcome to the IMMULITE® 2000 XPi System Sample Processing  Online Training course.  This course introduces you to: Processing patient samples using the LIS, monitoring status, and reviewing patient results Manually processing patient samples in certain situations, such as when the LIS is not operational Select Next to continue. Access the Sample Tubes in Racks screen, Display/Edit screen and Review screen to retrieve sample information Add a test to a sample in progress Assign various worklist options to samples Load and unload samples using rack loader Order unbarcoded patient samples Run patient samples using microsample tubes and tube top cups Run patient samples with or without an LIS Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations. You have completed the IMMULITE 2000 XPi System Sample Processing Online Training course.  Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz. Key Points: In this online training course, you learned how to:   Use the rack loader to load and unload samples without pausing the system. To load samples: Determine if the system is ready for a new rack by viewing the lights to the left of the rack loader door.  A solid red light on the rack loader indicates the instrument is not ready to accept a rack.  For example, if the rack loader is full, the light will remain red.  A solid green light on the rack indicates the instrument is ready to accept a rack. If the light is green, lift the rack loader door and set the sample rack into the sample tray. Close the rack loader door.The instrument automatically loads the rack onto the system when the sample carousel has an empty rack position. To unload samples, select an eject button from the Home screen, Rack Details screen, or Sample Tubes in Racks screen. When one of the eject buttons is selected, that rack will be automatically put into the rack loading area, as long as a rack is not already sitting there. Run patient samples with LIS orders.  These orders will automatically run if the tubes are barcoded. Order barcoded patient samples without an LIS.  If the system is not connected to an LIS, you must manually order tests using the IMMULITE 2000 XPi System software.  The samples must be loaded and the system must be in Run mode before the tests are ordered. To manually order test on patient samples: Load barcoded patient samples on the system Select Worklist Select Next Order Tests Select Accept Patient Review Display/Edit screen Identify sample status on the Sample Tubes in Racks screen, Display/Edit screen, and the Review screen. To access the Sample Tubes in Racks screen, select the Sample Tubes button from one of the Rack Details screens. The Sample Tubes in Racks screen displays all the racks and the tubes that are currently on the system. The color of the sample tube positions indicates the status of the samples.  The shape of the sample tube positions indicates the type of tube. It is important to select Update on the top right corner of the screen to display the most current information. To access the Display/Edit screen, select the Worklist button from the horizontal toolbar and then select the Display/Edit button. The Display/Edit screen displays a list of pending test orders, including the tube's sample carousel position and the status of that test.  After a test has been successfully pipetted, the status will indicate the time to completion for that test. It is important to select Update Screen on the bottom right corner of the screen to display the most current information. To access the Review screen, select Review from the horizontal toolbar.  A blank Review screen displays.  Results will appear on the screen when a time frame is selected. This screen can be used to review results from a sample previously run. Order unbarcoded patient samples.  Sample tubes that have missing or damaged barcodes must either be re-labeled or assigned an accession number. To order tests on unbarcoded patient samples: Load unbarcoded patient samples on the system Select Worklist Select New and enter accession number Order tests Assign tube position Select Accept Patient Review Display/Edit screen Run patient samples using microsample tubes and tube top cups.  On the IMMULITE 2000 XPi system, the dead volume of secondary and primary tubes is 250µl.  If you have a sample with volume less than 250µl, either microsample tubes or tube top cups can be used.  When you run either of these types of tubes, order the tests is the same you order secondary and primary tubes.  Tests can either be ordered using an LIS, or through the system software using the Worklist screen. The dead volume of microsample tubes is 50µl. Microsample tubes are identified on the system by a permanent barcode label attached to a reusable microsample tube holder.  A disposable 10 x 50 mm polystyrene sample tube is inserted into the holder.  Microsample tubes are placed in the same standard sample racks that are used to run secondary and primary tubes. The dead volume of tube top cups is 50-100µl. The IMMULITE 2000 XPi System allows you to pipette a small sample into an approved tube top cup and place the cup back in the original sample tube.  Tube top cups must be placed in a dedicated tube top sample rack.  The tube top racks are identified with a lower case letter on the rack barcode. The tube top rack has a capacity of 15 tubes. However, you can only load 8 shorter tubes (75 mm) in the front row and 7 taller tubes (100 mm) in the back row. Add a test to a sample in progress.  If the new test is being ordered from the LIS, a requery of the LIS can be initiated in two ways.  Either the sample barcode is reread by pausing the system and putting it back into Run mode, or the Requery button can be selected on the LIS screen.  If the new test is not being ordered from the LIS, you can order the test using the system software. To add a test to a sample that is in progress: Select Woklist. Ensure that Patient is highlighted in blue at the top of the screen. Select the New button to display a blank worklist. Enter the accession number for that sample into the accession field. Select the Test button to view the available tests screen.  Select the appropriate test(s) to run, and press OK. Select Accept Patient. Use several options that can be assigned to samples on the Worklist screen.  A test order can be assigned a STAT status, meaning that the test will have pipetting priority over the other tests in the worklist.  Also, a sample tube can be identified as either a secondary tube or a primary tube, which affects how the sample is pipetted.  There are additional options for dilutions which include ordering onboard dilutions on tests and identifying samples as being manually diluted. To indicate a STAT sample: Select STAT on the Worklist screen. Select the test name in the Tests Ordered box. (The test name turns red, indicating this test now has a STAT priority and runs first) Select Accept Patient. Tube Type Setting: Primary Tube: A blood collection tube in which the serum is separated from the blood cells by a gel barrier. The probe stops before penetrating the gel barrier. Secondary Tube  A sample tube in which the serum is aliquotted to a different tube.  The probe moves further into the tube before aspirating the sample. To run an onboard dilution: The appropriate assay diluent must be loaded on the sample carousel in a 16 x 100 mm tube with the diluent barcode. Select the Dilution button on the Worklist screen. Select the test name in the Tests Ordered box. Select the desired dilution factor. To run a manually diluted sample: The appropriate assay diluent must be used.The diluents are concentrated and must be diluted 1 part diluent to 1.5 parts de-ionized water before using it to dilute the sample. In the Worklist screen, select the Manual Dilution button. Type the manual dilution factor. Select OK. Select Next to continue. On the IMMULITE 2000 XPi System, samples may be loaded and unloaded without pausing the system. Loading Sample Racks Learn how to load sample racks.   Select the play arrow to begin the video.  When complete, select the X in the upper-right corner to close the window and continue. Unloading Sample Racks Learn how to unload sample racks. Tab TitleTextHome Screen  On the Home screen, there are purple eject buttons next to each sample rack icon.  When one of the eject buttons is selected, that rack will be automatically put into the rack loading area, as long as a rack is not already sitting there.    Rack Details Screen To access the Rack Details screen, select a sample rack icon from the Home screen. On the Rack Details screen, there is an Eject Rack button.  When the Eject Rack button is selected for a certain sample rack, that rack will be automatically put into the rack loading area, as long as a rack is not already sitting there.    Sample Tubes in Racks Screen To access the Sample Tubes in Racks screen, select the Sample Tubes button from one of the Rack Details screens. On the Sample Tubes in Racks screen, there are purple eject buttons next to each sample rack icon. When one of the eject buttons is selected, that rack will be automatically put into the rack loading area, as long as a rack is not already sitting there. When complete, select the X in the upper-right corner to close the window and continue.   Sample Tube Sizes Learn more about sample tube sizes. The following primary and secondary round bottom tube sizes may be used by the system:   12 x 75 mm 13 x 75 mm 16 x 75 mm 12 x 100 mm 13 x 100 mm 16 x 100 mm The sample volume required varies with the assay to be run and the number of replicates requested on that sample. The exact sample volume required for each test can be found in the corresponding instructions for use (IFU). An additional 250µl of sample is required for proper instrument operation.     When complete, select the X in the upper-right corner to close the window and continue. If the system is in Run mode, the IMMULITE  2000 XPi System will automatically start running barcoded patient samples with LIS orders. Bi-directional LIS system: A worklist is created when the LIS downloads the accession numbers, desired tests, and patient demographics to the system. Samples are not needed to create the worklist. The system matches the downloaded records to the barcoded samples placed on the system. Bi-directional Query LIS system: A worklist is created when the samples are placed on the system. The barcodes are read and the system requests the test orders for the samples onboard from the LIS host. The LIS downloads the orders and the system processes the samples. Select Next to continue. If the system is not connected to an LIS, you must manually order tests using the IMMULITE 2000 XPi system software. The samples must be loaded and the system must be in Run mode before the tests are ordered. Let's look at how to run barcoded patient samples without an LIS.   Ordering Barcoded Patient Samples Without an LIS Learn how to order barcoded patient samples without an LIS. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003784/sim_IMMXPi_RunBarcodenoLIS_9/sim_IMMXPi_RunBarcodenoLIS_9.swfHTML5 File:/content/generator/Course_90003784/sim_IMMXPi_RunBarcodenoLIS_9/index.htmlPDF File: There are several places within the IMMULITE 2000 XPi software where information on the status of your samples is displayed.   Identifying Sample Status Learn about identifying sample status. Tab TitleTextSample Tubes in Racks Screen To access the Sample Tubes in Racks screen, select the Sample Tubes button from one of the Rack Details screens.  The Sample Tubes in Racks screen displays all the racks and the tubes currently on the system.  The color of the sample tube positions indicates the status of the samples. The shape of the sample tube positions indicates the type of tube. NOTE:  It is important to select Update on the top right corner of the screen to display the most current information.  Display/Edit Screen To access the Display/Edit screen, select the Worklist button from the horizontal toolbar and then select the Display/Edit button. The Display/Edit screen displays a list of pending test orders, including the tube's sample carousel position and the status of that test.  After a test has been successfully pipetted, the status will indicate the time to completion for that test. NOTE:  It is important to select Update Screen on the bottom right corner of the screen to display the most current information. When complete, select the X in the upper-right corner to close the window and continue. The results review function is used to review results from a sample previously run. To access the Review screen, select Review from the horizontal toolbar. A blank Review screen displays. Results will appear on the screen when a time frame is selected. Review Screen Components Learn more about the Review screen. Base ImageHotspotsText BlocksImage FileTime Select Time to set the time frame searched. The default is the current date. When the Time button is selected, the Select Time Method window opens.  The options are to select a date range, today only, or all results.  After making your selection, select the ok button.  Search When the Search button is selected, the Search By window opens.  This option allows you to search for a particular result. The Search By window varies depending on the record type selected in the options area: patient, control, adjustor, or calibration verifier.  The Search button is not available if the All option is selected.    Results   Results are listed on the screen for one sample at a time. Print The Print button is used to print the results for the sample currently displayed on the screen. Options In the options area of the screen, select the type of result you would like to view:   All Patient Adjustor Control Verifier (for calibration verifier) Search When the Search button is selected, the Search By window opens.  This option allows you to search for a particular result. The Search By window varies depending on the record type selected in the options area: patient, control, adjustor, or calibration verifier.  The Search button is not available if the All option is selected.    Previous / Next  Use the Previous and Next buttons to scroll through the results, viewing the results for one sample at a time.  Sample tubes that have missing or damaged barcodes must either be re-labeled or assigned an accession number. Let's look at how to run unbarcoded patients. Loading Unbarcoded Patient Samples Learn about loading unbarcoded patient samples.   Select the play arrow to begin the video.  When complete, select the X in the upper-right corner to close the window and continue.    Running Unbarcoded Patient Samples Learn how to run unbarcoded patient sample. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003784/2 - sim_IMMXPi_UnbarcodedPatients_9/2 - sim_IMMXPi_UnbarcodedPatients_9.swfHTML5 File:/content/generator/Course_90003784/2 - sim_IMMXPi_UnbarcodedPatients_9/index.htmlPDF File: On the IMMULITE 2000 XPi System, the dead volume of secondary and primary tubes is 250µl.  There are two options for running tests using samples whose volumes are less than 250µl: Microsample Tubes Tube Top Cups When you run either of these types of tubes, order the tests the same way you order secondary and primary tubes.  Tests can either be ordered using an LIS, or through the system software using the Worklist screen. Note: It is recommended to run no more than five tests from a single microsample tube or tube top cup.   Using Microsample Tubes and Tube Top Cups Learn how to use microsample tubes and tube top cups. Tab TitleTextMicrosample Tubes Microsample tubes allow you to run small volumes of sample using specified aliquot tubes and a microsample tube holder.   The dead volume for microsample tubes is 50µl.   Microsample tubes are identified on the system by a permanent barcode label attached to a reusable microsample tube holder.  A disposable 10 x 50 mm polystyrene sample tube is inserted into the holder.  Microsample tubes are placed in the same standard sample racks that are used to run secondary and primary tubes.   After you load the microsample tubes on the sample carousel and put the system into Run mode, the samples are ordered the same way primary and secondary tubes are ordered.  The positions of microsample tubes are identified on the Sample Tubes in Racks screen by a square.  Tube Top Cups Tube top sample cups are also known as nesting cups or small sample cups (SSC). The dead volume of tube top cups is 50-100µl.   The IMMULITE 2000 XPi System allows you to pipette a small sample into an approved tube top cup and place the cup back in the original sample tube.  Tube top cups must be placed in a dedicated tube top sample rack.  The tube top racks are identified with a lower case letter on the rack barcode. The tube top rack has a capacity of 15 tubes. However, you can only load 8 shorter tubes (75 mm) in the front row and 7 taller tubes (100 mm) in the back row.  Note :  Reference the IMMULITE 2000 XPi Operator's Guide for the part numbers of tube top cups that are approved for use. After you load the tube top rack and put the system into Run mode, the samples are ordered the same as primary and secondary tubes.  The positions of tube top cups are identified on the Sample Tubes in Racks screen by a square with the letter "T".   When complete, select the X in the upper-right corner to close the window and continue. There may be times when it is necessary to add a test order to a sample that is already in progress with other tests. If the new test is being ordered from the LIS, a requery of the LIS can be initiated in two ways: The sample barcode is reread by pausing the system and putting it back into Run mode The Requery button can be selected on the LIS screen If the new test is not being ordered from the LIS, you can order the test using the system software.   Adding a Test to a Sample in Progress Learn how to add a test to a sample in process. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003784/sim_IMMXPi_AddTestToInProcessSample_9/sim_IMMXPi_AddTestToInProcessSample_9.swfHTML5 File:/content/generator/Course_90003784/sim_IMMXPi_AddTestToInProcessSample_9/index.htmlPDF File: There are several options that can be assigned to samples on the Worklist screen: STAT Secondary/Primary Tube Onboard Dilution Manual Dilution Assigning Worklist Options to Samples Learn how to assign worklist options to samples. Checklist TitleChecklist TypeChecklist ContentSTATsSimulationInstructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003784/sim_IMMXPi_STAT_9/sim_IMMXPi_STAT_9.swfHTML5 File:/content/generator/Course_90003784/sim_IMMXPi_STAT_9/index.htmlPDF File:Secondary/Primary TubesSimulationInstructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003784/sim_IMMXPi_TubeType_9/sim_IMMXPi_TubeType_9.swfHTML5 File:/content/generator/Course_90003784/sim_IMMXPi_TubeType_9/index.htmlPDF File:Onboard DilutionsSimulationInstructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003784/sim_IMMXPi_OnboardDilution_9/sim_IMMXPi_OnboardDilution_9.swfHTML5 File:/content/generator/Course_90003784/sim_IMMXPi_OnboardDilution_9/index.htmlPDF File:Manual DilutionsSimulationInstructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90003784/sim_IMMXPi_ManualDilution_9/sim_IMMXPi_ManualDilution_9.swfHTML5 File:/content/generator/Course_90003784/sim_IMMXPi_ManualDilution_9/index.htmlPDF File: