IMMULITE® 2000 XPi Immunoassay System Troubleshooting Online Training

Welcome to the IMMULITE® 2000 XPi System Troubleshooting Online Training Course. Utilizing troubleshooting tools will allow you to: Use error logs Resolve common operator errors Select Next to continue. Identify and Resolve Common Errors Locate and Review the Event Log and Day Error Log Recall the Tube Journey Upon successful completion of this course, you will be able to: Select Next to continue. Use the Calibration Data screen to access calibration data including scan date, time, and lot number for Microalbumin/Creatinine calibrations on the DCA Vantage® analyzer. Select the link of this page to learn how to view Calibration Data. Congratulations.  You have completed the IMMULITE 2000 XPi Troubleshooting online training course.  Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz. In this online training course, you learned how to: Recall the Tube Journey: Once a test is ordered on a sample, a reaction tube is drawn out of the Tube Hopper and travels to the Tube Indexer. A test-specific bead dispenses from the appropriate bead pack into the tube. The tube is transported to the top of the instrument where a shuttle pushes it into the pipetting position in the Incubator. The Sample and Reagent Pipettors draw the appropriate patient sample and reagent from the Sample and Reagent Carousels, and dispense them onto the bead. The test-specific bead, patient sample and reagent incubate together in the reaction tube.  When the incubation is complete, the tube will enter the Wash Station. Within the Wash Station, the tube spins on its vertical axis. 400 microliters of water dispenses onto the bead and the bead is spun again. This wash cycle is performed a total of four times. The tube moves under the Substrate Probe and 200 microliters of chemiluminescent substrate dispenses onto the bead. The substrate reacts with an alkaline phosphatase label that has become attached to the bead during its incubation period, producing light.  After five minutes in the Luminometer, the light reaction is read. The tube is pushed into the Luminometer disk and rotated in front of the PMT. Readings are then taken in units of counts per second, or CPS. The average of these light readings undergoes a series of calculations to produce a test result. The tube is transferred tot he Exit Chute, where it is dropped into the Solid Waste Container. Locate and Review the Event Log and Day Error Log: The Event Log records and displays error messages for the past 90 days. The Event Log is accessed from the IMMULITE 2000 XPi system startup screen. The Day Error Log contains all errors and system messages for a 24 hour period.  The Day Error Log is accessed on the Menu screen in the IMMULITE 2000 XPi system software. Identify and Resolve Common Errors: Diagnostic Jams:  When a jam occurs while running a Diagnostic, using the slave motor chart will help locate where the jam is occurring on the instrument. Also, running Home All Motors can sometimes help to clear the jam. MCP Routine Error: An MCP Routine Error occurs when a mechanical jam occurs during software initialization.  To resolve this error, check the Event Log to determine the component that jam and then if possible resolve the jam.  Once the jam is cleared, the software can be initialized. Carousel Jams: If reagent wedges, bead packs or sample racks are not positioned correctly, carousel jams may occur.  To resolve the error, investigate the carousel that is jammed and reseat any mispositioned reagents wedges, bead packs, or sample racks. Clear Tube Indexer Jam Ensure the instrument is in Pause mode. Open the front panel doors and verify that the proper reaction tubes are in the tube hopper. Pull out the pin that connects the orientation brush to the orientation chute. Lift the orientation brush and place it behind the orientation chute.  Be sure not to pull the orientation brush towards the front of the instrument to prevent the disconnecting of wires. Take out and discard all reaction tubes in the orientation chute including any tubes that are in the tube indexer itself. Place the orientation brush back in the orientation chute. Put the pin back in place to secure the orientation brush. Push the tube hopper back into place and place the instrument back into Run. Bulk Exit Chute Error: Ensure the instrument is in Stop mode and open the instrument top cover and the front panel doors. Remove the Solid Waste Container and check to see if it's full. Place a shallow container or an absorbent cloth over the Solid Waste Load Scale, toward the back wall.  Insert the Waste Chute Clean Out Tool into the Waste Chute opening from the bottom.  The Waste Chute Clean Out Tool should never be inserted into the top opening of the Waste Chute. Remove the tool.  The jammed Reaction Tubes and beads will fall onto the shallow container or absorbent pad. Locate the Waste Chute Cover on top of the instrument behind the Wash Spin Station.  Using a flashlight, look into the opening for the presence of Reaction tubes. If tubes are present, manually remove them. To ensure the blockage was removed, drop an empty Reaction Tube marked with an "X" down the Solid Waste Chute.  The marked Reaction Tube should travel freely down the chute to the tray or cloth in the Solid Waste Container area. If the marked Reaction Tube does not drop through, repeat the above steps to dislodge the jam. Reagent / Bead Screen Errors The Bead and Reagent Status screens are used to check the status of the reagent wedges, allergen wedges, and bead packs on board the system. To resolve a No Matching Bead or Reagent message on the Reagent / Bead screen: Determine the lot number of the bead pack or reagent wedge that is missing, by accessing the Detail screen for the component that is loaded onboard.  To display the Reagent or Bead Detail screens while the instrument is in Run mode, select the square for the reagent wedge or bead pack in the Reagent or Bead Status screen. Once the missing bead pack or reagent wedge with the correct lot number is located, load that component. Put the system back into Run mode. To resolve a Not in Database message on the Reagent / Bead screen: Scan the kit lot barcode into the database. Select the Pause button to put the system into Pause mode. Select the Run button to put the system back into Run mode. Tip Jam Reagent Pipettor To resolve this error: Identify the reagent position with the error. Remove the reagent wedge from the system. Check the glide. Reload the reagent wedge. Select Run. Reagent Probe False Level Sense Error To resolve this error: Identify the reagent position with the error. Remove the reagent wedge from the system. Check the top of the wedge. Check for bubbles in the liquid. Reload the reagent wedge. Select Run. Sample Pipettor Level Sense Error To resolve this error: Identify the sample position with the error. Remove the sample from the system. Add more sample or transfer to another tube. Reload the sample on the system. Bad Barcode If there are any tubes with damaged or missing barcodes, the sample rack that has those tubes will display as red on the Home screen.  The sample carousel positions for sample with bad barcodes can be seen on the following screens: Remove the tube from the system, fix the barcode, and reload the tube. Order tests on the unbarcoded tube by assigning the tube position.  Instructions for ordering tests on unbarcoded tubes can be found in the Sample Processing online training course. Damaged Probe To replace a damaged probe: Remove the probe tube retaining clip from the reagent/sample arm column. Unscrew the probe from the arm mount, and remove the probe from the arm. Unscrew the black probe extension from the manifold block, turning both the entire probe and extension piece together. Unscrew the probe extension from the probe. The extension piece will be reused to mount the new probe. Attach the probe extension to the fitting of the new probe. Attach the probe extension to the manifold block by turning both the entire probe and the extension piece together. Insert the probe into the arm mount and tighten securely. Verify the tubing is on the left side of the sample/reagent arm and secure the tubing into the probe tube retaining clip on the reagent/sample arm column. Clot Errors To resolve a gel or hanging clot: Open the pipettor cover Visually inspect the probe – If necessary, replace or clean the probe Close the pipettor cover Prime the DRDs Locate and remove the sample Select RUN to resume system operation  To resolve air in the system: Open the pipettor cover Visually inspect the probe Close the pipettor cover Select STOP Prime the DRDs Run the Clot Prime diagnostic Initialize the software and select RUN Select Next to continue. Understanding the steps that take place on the system while a test is running can be very useful when troubleshooting problems on the system.   Tube Journey Learn more about the tube journey.    Select the arrow to begin the video.  When complete, select the X in the upper-right corner to close the window and continue. The error logs on the IMMULITE 2000 XPi system: Day Error Log:  24 hours Event Log:  90 days Day Error Log Learn how to use the Day Error Log. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90004971/2 - SIM_IMMXPi_DayErrorLog_9/2 - SIM_IMMXPi_DayErrorLog_9.swfHTML5 File:/content/generator/Course_90004971/2 - SIM_IMMXPi_DayErrorLog_9/index.htmlPDF File: Event Log Learn how to use the Event Log. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90004971/3 - sim_IMMXPi_EventLog_9/3 - sim_IMMXPi_EventLog_9.swfHTML5 File:/content/generator/Course_90004971/3 - sim_IMMXPi_EventLog_9/index.htmlPDF File: How good are you at understanding clinical context? Listen to the following conversation between these two doctors. What was the result of Mrs. Smith's biopsy? Looks like an invasive ductal breast carcinoma, moderately differentiated with significant overexpression of HER-2/neu. The mammograms that she came with already didn't bode well. And the staging? MRI delivered better delineation of the lesion. There also might be 2 positive lymph nodes. PET-CT is scheduled for tomorrow. Jams may occur on the system while initializing and running diagnostic programs.  Using the slave motor chart will help locate where the jam is occurring on the instrument. Download and print a copy of a PDF about the Slave Motor Chart.   Diagnostic Jams Learn how to resolve diagnostic jams. Checklist TitleChecklist TypeChecklist ContentIdentify the slave and motor numberHTML If a jam occurs while a diagnostic program is running, the slave and motor number of the component that jammed will be shown on the top of the screen.  Use the Slave Motor Chart to determine the part of the system associated with the jam.   If the part of the system that jams is accessible to the operator (for example, the sample carousel or tube indexer), then investigate and try to resolve the problem.  After resolving the problem, contiue witht he following steps. If the part is not accessible to the operator (for example, the PMT shuttle or luminometer chain), then contact technical support. Select each checkbox to learn more about resolving diagnostic jams. Run Home All MotorsHTML Run the Home All Motors diagnostic if the problem was resolved using the Slave Motor Chart: Select Load Program at the top of the screen to exit the current diagnostic and return to the list of diagnostic programs.  Select Home All Motors from the list. Press Run in menu bar to start.   Determine if HomeAll Motors completes successfullyHTML If Program Complete displays at the top of the screen, then Home All Motors successfully completed.  Rerun the diagnostic that had jammed by selecting Load Program and selecting the diagnostic from the list.  If the system jams while Home All Motors is running, contact technical support. When complete, select the X in the upper-right corner to close the window and continue. If a mechanical jam occurs during software initialization, the software will close back to the Windows desktop. The Event Log will post the following errors: An error that the main control program (MCP) has shutdown. More specific error messages indicating the type of mechanical jam.   Resolve an MCP shutdown error Learn how to resolve an MCP shutdown error. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90004971/4 - sim_IMMXPi_ErrorDuringMCPRoutine_9/4 - sim_IMMXPi_ErrorDuringMCPRoutine_9.swfHTML5 File:/content/generator/Course_90004971/4 - sim_IMMXPi_ErrorDuringMCPRoutine_9/index.htmlPDF File: If reagent wedges, bead packs or sample racks are not positioned correctly, carousel jams may occur.   Carousel Jams Learn how to resolve carousel jams. Tab TitleTextReagent Carousel The following error message will require checking the reagent carousel for a jam: "Reagent Carousel has jammed during bar code read going into Pause." To resolve a reagent carousel jam, open the main reagent carousel door to ensure that the reagent wedges and carousel tray are loaded correctly in the carousel: Align the lines on the carousel tray and post and click tray into place. Tilt the barcode side of the wedge down as the wedge is placed in a carousel slot Press down on the narrow side of the wedge to lock it in place Ensure the glide moves freely Close the main reagent carousel door and select the Run button to put the system back into Run mode. Bead Carousel  The following error message will require checking the bead carousel for a jam: "Bead Carousel has jammed during bar code read going into pause." To resolve a bead carousel jam, open the bead carousel door to ensure that the bead packs are loaded correctly in the carousel: Place the bead pack in a carousel slot with the barcode facing out Tilt down the side of the bead pack opposite to the barcode label Snap down the barcoded side of the bead pack to lock it in place Close the bead carousel door and select the Run button to put the system back into Run mode     Sample Carousel The following error message will require checking the sample carousel for a jam: "Sample Carousel has jammed." To resolve a sample carousel jam, raise the main cover: Check that all sample tubes and diluent tubes are seated correctly in the sample racks. Check the movement of the pipettor is not obstructed. Check for unauthorized tube sizes in the sample racks. Check that the sample probe is not bent. Ensure that the sample racks are loaded correctly in the carousel. Close the main cover and select the Run button to put the system back into Run mode. When complete, select the X in the upper-right corner to close the window and continue.   If there is a jam at the tube indexer, the following error message will appear: "Tube Indexer jammed" Clearing a tube indexer jam Learn how to clear a tube indexer jam.   Select the play arrow to begin the video. When complete, select the X in the upper-right corner to close the window and continue. If the bulk exit chute is blocked, one of the following error messages will appear: "Cannot enter run with the bulk exit chute blocked." "Bulk Exit Chute blocked.  Entering PAUSE mode." "Bulk Exit Chute is blocked.  Entering STOP mode." Resolving Bulk Exit Chute Errors Learn how to resolve the bulk exit chute error.   Select the play arrow to begin the video. When complete, select the X in the upper-right corner to close the window and continue. Errors involving reagent wedges and beads will be reflected in the Bead and Reagent Status screens. The status of each position is indicated by the color of its square: White = No errors Gray = Empty position Light brown = Warning (few tests remaining, overdue adjustment) Red = Error Condition (no tests remaining, never adjusted, no matching bead pack or reagent wedge, not in database)   Reagent/Bead Screen Errors Learn about the No Matching Bead/Reagent and Not in Database error conditions. Tab TitleTextNo Matching Bead or Reagent In order to run a test on the system, both a bead pack and a reagent wedge from the same kit lot must be loaded.  If either kit component for a particular lot number is missing, there will be an error message on the Reagent or Bead Status screen.  For example, if a bead pack is loaded, but the corresponding reagent wedge is missing, then the Bead Status screen will display the message "No Matching Reagent" on the position for that bead pack. To resolve this error: Determine the lot number of the bead pack or reagent wedge that is missing, by accessing the Detail screen for the component that is loaded onboard.  To display the Reagent or Bead Detail screens while the system is in Run mode, select the square for the reagent wedge or bead pack in the Reagent or Bead Status screen. Locate and load the missing bead pack or reagent wedge with the correct lot number on the system Select the Run button to put the system  into Run mode. Not In Database The kit lot barcode should be scanned into the database before loading beads and reagents onto the system. If beads and reagents are loaded for a new lot and the system is put into Run mode before the kit lot barcoded is scanned, the message "Not in Database" will appear in the box for those components in the Bead and Reagent Status screens. To resolve this error: Scan the kit lot barcode into the database. Select the Pause button to put the system into Pause mode. Select the Run button to put the system back into Run mode. When complete, select the X in the upper-right corner to close the window and continue. If the reagent pipettor cannot access the reagent wedge, two error messages will appear: "Tip Jam Reagent Pipettor" indicates that the reagent pipettor could not access a reagent wedge properly. "Marking Reagent Pack as Bad" indicates which reagent wedge has the error. Tip Jam Reagent Pipettor Learn how to resolve the tip jam reagent pipettor error. Checklist TitleChecklist TypeChecklist ContentIdentify the reagent position with the errorHTML When the reagent tip jam error occurs, the error messages "Tip Jam Reagent Pipettor" and "Marking reagent pack as bad" will appear on the screen.   The position of the reagent pack with the error can be determined on the following screens: Display/Edit screen:  the test with the error will have a status of "Reagent Err" Home screen:  the reagent wedge position will appear red Reagent screen:  the reagent wedge position will appear red, with the message "Instrument Error" Select each checkbox to learn how to resolve the tip jam reagent pipettor error. Remove the reagent wedge from the systemHTML   Open the reagent carousel cover and wait for the system to enter Reagent pause.  Then, select the reagent position from the Reagent screen to rotate the reagent carousel to that position.  Remove the reagent wedge. Check the glideHTML   Ensure the glide moves back and forth smoothly on top of the wedge.   Reload the reagent wedgeHTML   Ensure that reagent wedge is loaded correctly: Tilt the barcode side of the wedge down as the wedge is placed in a carousel slot Press down on the narrow side of the wedge to lock it in place Ensure the glide moves freely  Select RunHTML   Select the Run button to put the system back into Run mode.  The system will automatically try to run the test again. When complete, select the X in the upper-right corner to close the window and continue.  If the reagent pipettor level senses on a film at the top of a reagent wedge or on a bubble, two error messages will appear: "Reagent False Level Sensed." indicates that the reagent pipettor level sensed at an unexpected height. "Marking Reagent Pack as Bad" indicates which reagent wedge has the error. Reagent Probe False Level Sense Error Learn how to resolve the reagent false level sense error. Checklist TitleChecklist TypeChecklist ContentIdentify the reagent position with the errorHTML   When the reagent level sense error occurs, the error messages "Reagent False Level Sensed" and "Marking reagent pack as bad" will appear on the screen.  Also, the position of the reagent pack with the error can be determined on the following screens: Display/Edit screen:  the test with the error will have a status of "Reagent Err" Home screen:  the reagent wedge position will appear red Reagent screen:  the reagent wedge position will appear red, with the message "Instrument Error" Select each checkbox to learn how to resolve the reagent false level sense error.  Remove the reagent wedge from the systemHTML   Open the reagent carousel cover and wait for the system to enter Reagent pause.  Then, select the reagent position from the Reagent screen to rotate the reagent carousel to that position.  Remove the reagent wedge.  Check the top of the wedgeHTML   Check for a reagent fluid film under the wedge glide.  Ensure the top of the wedge and the glide are dry.  Check for bubbles in the liquidHTML   Ensure there are no bubbles in the reagent. Reload the reagent wedgeHTML   Ensure that reagent wedge is loaded correctly: Tilt the barcode side of the wedge down as the wedge is placed in a carousel slot Press down on the narrow side of the wedge to lock it in place Ensure the glide moves freely  Select RunHTML   Select the Run button to put the system back into Run mode. The system will automatically try to run the test again. When complete, select the X in the upper-right corner to close the window and continue. If there is not enough sample in the tube to run a test, two error messages will appear: "Sample pipettor did not level sense": indicates that the sample pipettor has reached the tube bottom and has not level sensed "Marking sample tube as bad": indicates which tube has the error Sample Pipettor Level Sense Error Learn how to resolve the sample pipettor did not level sense error. Checklist TitleChecklist TypeChecklist ContentIdentify the sample position with the errorHTML   When the sample level sense error occurs, the error messages "Sample pipettor did not level sense" and "Marking sample tube as bad" will appear on the screen.  Also, on the Display/Edit screen, the sample with the error will have a status of "Sample Error".  On the Home screen, the sample rack with that tube will appear as red, indicating a problem with one of the tubes in that rack. Select each checkbox to learn how to resolve the sample pipettor did not level sense error. Remove the sample from the systemHTML Once the other tubes on that sample rack are in process, remove the sample rack from the system.  Tubes that are in process will have a time to completion on the Display/Edit screen.  To eject the sample rack, select the purple eject button next the rack icon for the sample rack that contains the tube with the error.  Add more sample or transfer to another tubeHTML   If the problem is short sample and more sample is available, add more sample to the tube. If more sample is not available, the sample can be transferred to a tube top cup or a microsample tube if enough sample is available.  The dead volume of microsample tubes is 50uL, while tube top cups have a dead volume of 100uL.  For more information on processing these samples, see the Sample Processing course.  Reload the sample on the systemHTML   When the sample is reloaded on the system, it will automatically be processed and the system will try to run the tests again. When complete, select the X in the upper-right corner to close the window and continue.  The sample or reagent probe will need to be replaced if it is damaged. The probe angle diagnostics can be used to determine if a probe is damaged: Sample probe angle - weekly maintenance Reagent probe angle - monthly maintenance Additionally, if a problem with one of the probes is suspected, either diagnostic can be run at anytime.  Refer to the Routine Maintenance course for information on running these diagnostics. Replace the sample or reagent probe Learn how to replace the sample or reagent probe.    Select the play arrow to begin the video. When complete, select the X in the upper-right corner to close the window and continue. If there are any tubes with damaged or missing barcodes, the sample rack that has those tubes will display as red on the Home screen. The sample carousel positions for sample with bad barcodes can be seen on the following screens: Sample Tubes in Racks screen - a white color indicates unbarcoded tubes Display / Edit screen - if an order exists for an unbarcoded tube, the status will be "No Sample" To resolve this error, either: Remove the tube from the system, fix the barcode, and reload the tube. Order tests on the unbarcoded tube by assigning the tube position.  Instructions for ordering tests on unbarcoded tubes can be found in the Sample Processing online training course. Select Next to continue.   Sample clots occur when the clot remains in the sample. The system successfully draws a slug of air after the clot is detected, indicating the pipettor is not obstructed. The system will remain in Run mode and continue processing other samples. The operator needs to remove the clotted sample, fix the problem, and reload the sample to run those tests. A clinging clot occurs when the clot is stuck to the sample pipettor. The system is unsuccessful when it draws a slug of air, indicating the pipettor is obstructed. In addition to the error messages above, another error is posted:  "Clot detected in sample tube.  Clean exterior of sample probe before resuming operation." The system enters a pause state in which no new samples will be pipetted until the clot is removed. There are three possible causes for this type of error message: Gel clot:  The pipettor entered the gel in a gel separator tube. Hanging clot:  A clot is hanging on the end of the pipettor. Air in the system:  Air is present in the clot detection module. Gel Clot Learn how to resolve a clinging clot error that is caused by a gel clot. Checklist TitleChecklist TypeChecklist ContentOpen the pipettor coverHTML    Lift open the pipettor cover. Visually inspect the probeHTML    If the probe entered the gel barrier of a tube, replace the probe.  Refer to the "Damaged Probe" topic earlier in this course. Close the pipettor coverHTML  Close the pipettor cover. Prime the DRDsHTML   Select the PRIME button on the vertical toolbar to prime the DRDs. Use the DRD/Substrate door to view the DRDs as they prime and ensure there is no air in the lines. Locate and remove the sampleHTML   On the Display/Edit screen, the sample with the clot will have a status of "Sample Error".  On the Sample Tubes in Racks screen, the position for that sample will be pink, indicating a sample error. On the Home screen, use the eject button for the sample rack with the sample error to remove that sample from the system.  Select RUN to resume system operationHTML    When complete, select the X in the upper-right corner to close the window and continue.  Hanging Clot Learn how to resolve a clinging clot error that is caused by a hanging clot. Checklist TitleChecklist TypeChecklist ContentOpen the pipettor coverHTML    Lift open the pipettor cover. Visually inspect the probeHTML   If there is a hanging clot, use a lint-free wipe to remove the clot with a downward wiping motion. CAUTION:  Do not insert anything into the probe. Permanent damage may occur.  Close the pipettor coverHTML   Close the pipettor cover.  Prime the DRDsHTML   Select the PRIME button on the vertical toolbar to prime the DRDs. Use the DRD/Substrate door to view the DRDs as they prime and ensure there is no air in the lines. Locate and remove the sampleHTML   On the Display/Edit screen, the sample with the clot will have a status of "Sample Error".  On the Sample Tubes in Racks screen, the position for that sample will be pink, indicating a sample error. On the Home screen, use the eject button for the sample rack with the sample error to remove that sample from the system. Select RUN to resume system operationHTML   When complete, select the X in the upper-right corner to close the window and continue.    Air in the System Learn how to resolve a clinging clot error that is caused by air in the system. Checklist TitleChecklist TypeChecklist ContentOpen the pipettor coverHTML   Lift open the pipettor cover.  Visually inspect the probeHTML    If there is not a hanging clot or gel on the probe, continue with the steps to remove air from the lines. Close the pipettor coverHTML Close the pipettor cover.  Select STOPHTML    Select the STOP button on the vertical toolbar.  Air in the system can impact test results and therefore any tests in progress will have to be rerun after the air is primed out of the system. Prime the DRDsHTML   Select the PRIME button on the vertical toolbar to prime the DRDs. Use the DRD/Substrate door to view the DRDs as they prime and ensure there is no air in the lines.  Run the Clot Prime diagnosticHTML Log off of the system. Initialize diagnostics. Select Clot Prime diagnostic. Allow the diagnostic to run for several minutes to ensure that all of the air is eliminated.  Initialize the software and select RUNHTML   Initialize the IMMULITE 2000 XPi system software and selct RUN to resume system operation. When complete, select the X in the upper-right corner to close the window and continue.