PEPFAR Quality Control and Method Validation Activity 3 Job Aid 1: SE vs. RE

Job Aid 1: SE vs. RE 307 Effective Date: 12/6/2016 HILS1746 SYSTEMATIC ERRORS (SE) RANDOM ERRORS (RE) Consistent / Constant Change O Inconsistent Change O Change in reagent lot - Improperly mixed/dissolved - Change in calibrator lot reagent Wrong calibrator values Air bubbles in reagents and - Improperly prepared reagents reagent lines, sampling or reagent -Deterioration of reagents or syringes control material Pipette tips not fitting - Inappropriate storage of properly reagents A clogged pipettor (clot) - Pipette maladjustments or Imprecise pipettor misalignment Unstable temperature and Change in temperature of incubation incubators and reaction blocks Unstable power supply - Failing light source Poor operator technique Changes in procedure from - Defective disposable consumables one operator to another Sample evaporation Outdated reagents - Improper mixing of processed - Inadequately cleaned samples glassware Incorrect reconstitution of the control material 27