PEPFAR Quality Control and Method Validation Activity 7 Worksheet 1: How to Calculate TE

HILS1746 Effective Date: 12/6/2016 Worksheet 1: Calculate TE 703 Analyte Mean True Value SD (target) WBC, Total Count (cell * 109/L) 18.0 18.2 1.2 Potassium (mmol/L) 3.8 3.5 0.1 Creatinine (umol/L) 90 90 Platelet Count (cell * 109/L) 160 150 Glucose (mmol/L) 6.5 6.7 0.2 Calcium (mmol/L) 2.26 2.25 0.03 69 Effective Date: 12/6/2016 HILS1746 Worksheet 1: Calculate TE 703 Directions: Using the key numbers supplied on the previous page, complete the following table. The first two rows have been populated as a guide. Analyte Bias Bias TE in units %CV Abs Bias % TE in % (abs bias) x -True Value | x -True Value| Abs Bias + (1.65*SD) (SD /x )*100% (Abs Bias /True Value) *100% Abs Bias % + (1.65* %CV) WBC 18.0 - 18.2 = 0.2 0.2 + 1.65 *1.2 (1.2 /18.0)*100% (0.2 / 18.2)* 100% 1.1% + 1.65 *6.7% -0.2 = 2.2 = 6.7% =1.1% =12.2% Potassium 3.8 - 3.5 0.3 0.3 + 1.65 *0.1 (0.1/ 3.8)*100% (0.3/ 3.5) * 100% 8.6% + 1.65 *2.6% = 0.3 =0.5 = 2.6% = 8.6% = 12.9% Creatinine Platelet Glucose Calcium 70