Sysmex® CA-1500 System Maintenance Online Training

Welcome to the Sysmex® CA-1500 System Maintenance Online Training course. Instrument maintenance is key in keeping your Sysmex® CA-1500 System functional. It is important to learn instrument maintenance procedures such as: Daily maintenance Weekly maintenance 30/90 day maintenance As needed maintenance These maintenance procedures keep your Sysmex® CA-1500 System up and running. More information on maintenance can be found in the Sysmex® CA-1500 System Operator's Manual. Select Next to continue. Upon successful completion of this course, you will be able to: Identify daily maintenance tasks Identify weekly maintenance tasks Identify 30/90-day maintenance tasks Identify as-needed maintenance tasks Select Next to continue. Congratulations. You have completed the Sysmex® CA-1500 Maintenance online training course. Listed below are the key points that have been presented. Take time to review the material before you proceed to the final quiz. Identify daily maintenance tasks To perform daily maintenance, clean sample & reagent probes, discard used reaction tubes, discard waste, remove condensation from reagent tray holders, and check & discard trap chamber fluid. Identify weekly maintenance tasks To perform weekly maintenance, prime the hydraulic line and clean the instrument interior & exterior. Identify 30/90-day maintenance tasks To perform 90-day maintenance, perform an LED calibration. If your lab runs dFbg assays, perform the LED calibration every 30 days. Identify as-needed maintenance tasks To perform as-needed maintenance, adjust pressure, replenish reagents, replenish reaction tubes, replace sample plates, replenish rinse solution, replace fuses, replace halogen lamp, and replace the cap-piercer (if installed). Select Next to continue. To ensure proper performance of the Sysmex® CA-1500 System, perform the daily maintenance tasks shown below: Clean sample & reagent probes Discard waste Check & discard trap chamber fluid Discard used reaction tubes Remove condensation from reagent tray holders Maintenance Tips Turn off the power and unplug the power cord before removing condensation from reagent trays and checking/discarding trap chamber fluid Daily maintenance tasks should take you less than 10 minutes to complete After completing daily tasks, be sure to update the Maintenance Checklist Daily Maintenance Learn about daily maintenance. Tab TitleTextClean Probes To clean the sample & reagent probe the technologist would perform a Rinse Probe. To execute a Rinse Probe: From the Main Menu. Select Rinse Probe. Select Execute. Wipe the sample probe with an alcohol swab if needed.   Discard waste The waste container should be emptied as a part of daily maintenance. To empty the waste container: Open the cap from the waste tank. Turn the cap counterclockwise and take out the float switch carefully avoiding splashes or drips. Discard waste fluid and empty the waste container. Insert the float switch into the waste container, and turn the cap clockwise. Make sure that the tubing is securely connected and is not kinked.    Check Trap Chamber It is important to check and discard fluid in the trap chamber daily. To check and discard trap chamber fluid: Turn OFF the power and wait approximately 30 seconds. The trap chamber is located on the right side of the instrument. Turn the trap chamber clockwise and remove it. Discard the collect fluid (if any), and re-attach the trap chamber. Make sure that the float is inside and float direction is correct. (float should have pointed end facing up in the trap chamber)    Empty Tube Trash Used reaction tubes are automatically thrown in the trash box. At completion of every 200 tests, or at least once every 24 hours, discard the used reaction tubes from the trash box and clean it with tap water. While the power is ON and the instrument status is "Ready", pull the trash box slightly and then draw it out from the left side panel. ("Tube Trash has not been set" message will appear) Discard used reaction tubes. Clean the reaction tube trash box with tap water, and thoroughly wipe off moisture from the reaction tube trash box. Place reaction tube trash box back into the left side panel.   Remove Condensation Every 24 hours, check to see if condensation has formed on the reagent trays. To remove condensation from the Reagent Tray Holders: Turn OFF the power. Open the light shield. Pull out the reagent trays. Using a paper towel, remove the condensation from the cooling unit. Reinsert the reagent trays. Close the light shield lid. Turn ON the power. When complete, select the X in the upper-right corner to close the window and continue.   Weekly maintenance tasks include: Priming the Hydraulic Line Cleaning the Interior & Exterior of the instrument After completing weekly tasks, be sure to update the Maintenance Checklist.   Weekly Maintenance Learn about weekly maintenance. Tab TitleTextPrime Hydraulic Line As a part of weekly maintenance, or whenever the instrument has been left without operation for a day or more regardless of its power ON or OFF, refill the hydraulic line with rinse solution. To prime the hydraulic line: Check that there is sufficient rinse solution in the rinse container and waste container is empty. From the Main Menu screen, press the Special Menu key. Select the Maintain key. Select the Rinse & Prepare key. Select the Execute key.  Priming with rinse solution will start. When complete press the Return key.    Clean Exterior To ensure the instrument will be maintained in good condition, it is good to clean the instrument once a week. To clean the exterior: Turn OFF the power. Use a paper towel moistened with water and a neutral detergent, wipe off the exterior; then wipe again with a soft, dry paper towel. .    Clean Interior In addition to cleaning the exterior of the instrument, it is also important to clean the interior of the instrument at least once a week to ensure the instrument stays in good condition.   To clean the interior: Open the light shield lid. Pull out the reagent trays. Use a paper towel moistened with water and a neutral detergent, wipe over the interior; then wipe again with a soft, dry paper towel. Clean the removed reagent trays the same way. Insert the reagent trays. Close the light shield lid. When complete, select the X in the upper-right corner to close the window and continue.   Perform an LED calibration every 90 days—or—if your laboratory runs derived fibrinogen (dFbg) assays, perform the LED calibration every 30 days. To perform an LED calibration: From the Main Menu select Special Menu > Maintain > Calib. Lamp and LED > Calib. LED. Enter the Target Value found on the CaCalS sheet. Select the vial type as CaCalS and place in position D1 using adapter #110. Place CA Clean I in position E3. Select Execute. 30/90 Day Maintenance Tips: The Sysmex® CA-1500 System displays a message when powered up if an LED calibration is due. If an LED fails calibration, the detector status is shown as ERR instead of OK. A clotting detector well with a failed LED can be locked out and not used, but testing continues with the other seven clotting detector wells. After completing LED calibration, be sure to Set the calibration before quitting the screen. After completing LED calibration, be sure to update the Maintenance Checklist. Select Next to continue. Some maintenance tasks need to be performed on an as-needed basis, usually in response to an error message that appears on the touch screen. As-needed maintenance includes: Adjusting pressure Replenishing reaction tubes Replacing halogen lamp Replenishing reagents Replenishing rinse solution Replacing cap-piercer Replacing sample plates Replacing fuses As-needed Maintenance Learn about as-needed maintenance tips. As-Needed Maintenance Tips Wait for the lid light to turn green before opening the light shield lid to replace sample plates. Load reaction tubes up to the red fill-line inside the hopper—overfilling will cause jams. Be sure to turn off the power and unplug the power cord before replacing fuses, replacing the halogen lamp, or replacing the cap-piercer sample probe (if installed). After replacing the halogen lamp, you must perform a lamp calibration. After completing as-needed tasks, be sure to update the Maintenance Checklist. When complete, select the X in the upper-right corner to close the window and continue. As-Needed Maintenance Learn about as-needed maintenance. Tab TitleText0.10 MPa Pressure The pressures from the built-in pneumatic unit are adjusted to 0.22 MPa (2.2kg/cm2) and 0.10 MPa (1.0kg/cm2). These pressures are constantly monitored by pressure sensors. If an abnormality is detected, an error message will appear.  To adjust the 0.10 MPa (1.0kg/cm2) pressure: From the Main Menu Select Special Menu > Maintain > Pressure Adjust. Pull the 0.10 MPa (1.0kg/cm2) adjustment knob toward you to unlock. Adjust the pressure by turning the adjustment knob. The pressure will rise when turning clockwise, and lower when turning counter clockwise. After adjustment, press the knob in to lock.   0.22 MPa Pressure The pressures from the built-in pneumatic unit are adjusted to 0.22 MPa (2.2kg/cm2) and 0.10 MPa (1.0kg/cm2). These pressures are constantly monitored by pressure sensors. If an abnormality is detected, an error message will appear.  To adjust the 0.22 MPa (2.2kg/cm2) pressure: From the Main Menu screen, press Special Menu > Maintain > Pressure Adjust. Loosen the fixing screw on the adjustment knob by turning screwdriver counterclockwise while the adjustment knob is held to prevent from rotating. Adjust the pressure by turning the adjustment knob. The pressure will rise as you turn clockwise, and lower when turned counter clockwise. After the adjustment, tighten the fixing screw while keeping the adjustment knob from rotating.   Reaction Tubes If the reaction tubes run short during analysis, the error "Replenish Reaction Tubes" will appear. If you supply the instrument with reaction tubes while the message is displayed, the analysis will continue to interrupt. To replenish reaction tubes: Open the tube hopper by pressing the front part of the cover to pop it up; then open. Replenish reaction tubes. The reaction tube hopper will hold up to 300 tubes. Close the reaction tube hopper lid. Halogen Lamp The average lifetime of the lamp is 2000 hours. It is recommended to replace the lamp between 1000 hours and 2000 hours of use. To replace the lamp: From the Main Menu screen, select Special Menu > Maintain > Calib. Lamp & LED > Calib. Lamp. Select Not Analyzed if chromogenic/immunologic methods are not used. Select Analyzed if these methods are used. Select Return. Turn OFF the power and unplug the power cord. Remove exterior lamp cover. Remove the lamp cover. Press the clamp of the connector and remove the connector. Remove the lamp from the lamp holder. Install new lamp in the reverse order of removal. Allow lamp to burn in for 30 minutes before calibrating lamp.   Replenish Reagents If the reagent runs out during analysis, an error message "Insufficient Reagent" will appear and the analysis will be interrupted. To replenish reagents: From the Main Menu, select Set Reagents. Open the light shield. Replace with new reagent in reagent holder. If the Reagent Volume Alarm is set to "Reagent Volume", enter the preset reagent volume. Close the light shield lid. Press the Resume key. Analysis will continue. When complete, select the X in the upper-right corner to close the window and continue.   As-needed Maintenance Learn about additional as-needed maintenance. Tab TitleTextReplenish Rinse If the rinse solution runs out during an analysis an error "Replenish Rinse fluid" will appear and the analysis will be interrupted. To replenish rinse fluid: A "Being Interrupted" message will appear. After a few moments the interruption process is completed. Turn the cap counterclockwise, open the rinse container cap that does not have tubing and float switch connected. (If using cubed reagent grade water like the image on the right: remove cap with tubing and float. Be sure float does not touch any potentially contaminated surfaces.) Fill up the rinse container with rinse solution (distilled water). Tighten the cap clockwise to close. Make sure that the tubing is securely connected and is not kinked. Press the Resume key. The analysis will continue.    Replace Cap-piercer When the count of cap piercing analysis exceeds 30,000 times, replace the piercer. The message "Replace Piercer" will be displayed when the count exceeds 30,000. To replace the cap-piercer: From the Main Menu, select Special Menu > Maintain > Replace Piercer. Close the light shield lid, and then select Execute. A message will prompt the user the turn OFF the power. Turn OFF power, and replace cap-piercer via procedure in the systems operators manual.   Replace Sample Plates If unused wells in the sample plate run out during an analysis, an error message "Sample Plate completely used. Replace Plate." will appear and analysis will be interrupted. To replace sample plate: A "being interrupted" message will appear. Wait a few moments until the interruption process is completed. When the system is ready for replacing plates, a message will appear. Open the light shield lid. Removed the sample plate that you are replacing, and set an unused, fresh sample plate in its place. Close the light shield lid. Press the Resume key. The analysis will continue.     Replace Fuses If a fuse is blown, replace the fuse. To replace a fuse: Turn OFF the power and unplug the power cord. Using a regular screwdriver, press the notch upward and pull out the fuse holder cap. Replace the fuse and attach the fuse holder cap to the instrument. When complete, select the X in the upper-right corner to close the window and continue.