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Sysmex® CA-1500 System Reagent Management Online Training

Identify main areas of the reagent information menu and define a reagent within the system software.  This clinical laboratory training qualifies for continuing education units (CEU).

Welcome to the Sysmex® CA-1500 System Reagent Management Online Training course. In this course, you’ll learn how use the Reagent Information Setting screen to define all the reagents used on the instrument--these reagents include: Test method reagents Control plasmas Calibrators Factor deficient plasmas Rinse reagents Buffers Information that the system needs about each reagent includes lot number, expiration date, initial volume, vial type, and alarm limit settings. On an as-needed basis, you'll use the Reagent information screen to initially define reagents to the system. On a daily basis, you'll use the Consumables screen to verify that the data for the reagent that you are loading matches the data defined in the system. Select Next to continue. Upon successful completion of this course, you will be able to: Describe the Sysmex® CA-1500 System reagent management process Identify key areas of the Reagent Information Setting screen Describe how to define reagents Select Next to continue. Congratulations. You have completed the Sysmex® CA-1500 System Reagent Management online training course. Listed below are the key points that have been presented. Take time to review the material before you proceed to the final quiz. Describe the Sysmex® CA-1500 System reagent management process The reagent management process involves two main tasks: defining reagents using the Reagent Information Setting screen, and verifying reagent information using the Consumables screen. Identify key areas of the Reagent Information Setting screen Use the Reagent Information Setting screen to set the following reagent parameters: reagent ID and name, lot number, expiration date, initial volume, alarm & interrupt limit settings, and vial type. Control plasmas also require data for QC file and QC level settings. Demonstrate how to define reagents To define reagents, select one of six reagent categories, and then use the Reagent Information Setting screen to set reagent parameters. You can define reagents manually, but the fastest way to set reagent information is to scan the barcode label from the Consumables Screen—this automatically sets reagent ID, name, lot number, barcode ID, initial volume, alarm and interrupt Limits, and vial type. Select Next to continue. The Sysmex® CA-1500 System organizes reagents into six categories: Reagent, Factor Deficient Plasma, Buffer, Rinse, Control Plasma, and Calibrator. Reagent Management allows the system to monitor which reagents are onboard the instrument as well as monitor reagent volumes during processing. Managing reagents involves the following tasks: On a daily basis, use the Consumables screen to verify that the data for the reagent that you are loading matches the data defined in the system. Data available on the Consumables screen is "pulled from" the data that you initially entered on the Reagent Information Setting screen. (Main Menu > Set Reagents) On an as-needed basis, use the Reagent Information/Type Selection screen to select the reagent category to define, and then use the Reagent Information Setting screen to initially define the reagent to the system. This task includes adding new reagents as well as editing existing reagent data. (Main Menu > Settings > Analysis Settings > Reagent Info > Select category) Reagent Information and Consumables Screens Learn about Reagent Info/Consumable screens. On an as-needed basis, define reagents into the system. To define reagents, first select the reagent category that you want to define using the Reagent Information/Type Selection screen which displays six reagent categories. On an as-needed basis, use the Reagent Information Settings screen. The screen shown here defines a reagent in the Reagent category. On a daily bases, use the Consumables screen to verify that the data for the reagent that you are loading matches the data previously defined in the system. When complete, select the X in the upper-right corner to close the window and continue. It's important to accurately define reagents so that the system can monitor reagent volumes during processing as well as eliminate false alarms, such as Insufficient Reagent due to an incompatible vial type. To define a reagent, use the Reagent Information Setting screen. The data that the system requires depends on the reagent category:  Required reagent parameters include: reagent ID and name, lot number, barcode lot number, expiration date, initial volume, alarm & interrupt limit settings, and vial type. Control plasmas also require QC file and QC level parameters defined in the system. Reagent Barcode IDs Learn about entering reagents with a barcode ID. Each Siemens Healthcare Diagnostics reagent typically has a barcode on the bottle label that identifies both the reagent name and its lot number. A typical barcode lot number is a 6-digit number, for example: 526940 (5269 = product ID for Dade® Innovin® reagent and 40 = lot number). If the barcode on the bottle or box is an 8-digit number: 52694035 (526940 = barcode lot number, 3 indicates the vial size, and the last digit is a check-sum digit). When complete, select the X in the upper-right corner to close the window and continue.   Use the Reagent Information Setting screen to define a reagent in a selected reagent category. Let's take a closer look at the data displayed on the Reagent Information Setting screen when you've pressed the Reagent category key. Reagent Information Setting Screen Learn about areas on the Reagent Information Setting screen. Base ImageHotspotsText BlocksImage FileA reagent name is an 8-character field that easily identifies the reagent, such as PT INN for Dade® Innovin® reagent used for PT testing. The name can be manually entered using the Select Reagent window or by scanning the reagent barcode label using the hand-held wand. This method sets both the reagent ID and reagent name parameters automatically.  The reagent ID is a 4-character field that identifies a unique reagent. Siemens reagents use numbers in the 5000 to 6000 range. The first four digits of the barcode represent the reagent ID number. A new reagent can be added anywhere in the reagent list by selecting a reagent name from the Select Reagent window--this method sets both the reagent ID and reagent name parameters automatically. You can also select Other from the Select Reagent window and identify a non-Siemens reagent.You can enter a reagent lot number by manual entry using the lot number listed on the reagent vial label. Reagent lot numbers can comprise up to 12 characters.  The barcode ID identifies the reagent when using the barcode wand to enter or verify reagent addition from the Consumables screen in Set Reagents. The scanned barcode ID must match the barcode ID entered here. The first four digits of the barcode ID must also match the reagent ID.To manually define the reagent expiration date, enter the U.S. setting of mm/dd/yyyy format using the keypad displayed on the screen. This information is not contained as part of the reagent barcode.  Press Select Type to open the Reagent Information/Type Selection screen, where you can select another reagent category such as Factor Deficient Plasma, Control Plasma, Buffer, and others.  Press Add to open the Select Reagent window, where you can select a reagent from a selection list to add a new reagent or choose a reagent new to the system (such as adding a new control to the selection list of control plasmas). When you add a new reagent, only the Reagent ID and Name parameters are defined when you press the Add key. To define other parameters for a newly added reagent, press the Change key.  Select a reagent parameter, and then press Change to edit a current reagent parameter setting, or to enter parameter settings for a reagent that you have newly defined. To change a parameter’s value, press Change and then use the numeric or alphanumeric keypads that appear to edit parameter settings.  Select a reagent and then press Delete to delete the reagent parameter information. The entire row of information will be deleted.  Press Return to return to the Reagent Info. Submenu.Press the Other Param key to display additional reagent parameters, such as vial type, initial volume, alarm limit and interrupt limit.  Press the Up or Down arrow keys to select a reagent parameter in a row so that you can enter, verify, edit, add, or delete reagent information.  Press the Left or Right arrow keys to select a reagent parameter in a column so that you can enter, verify, edit, add, or delete reagent information. You can also move between the two reagent screens.  One of six reagent categories appears here: Reagent, Factor Deficient Plasma, Buffer, Rinse, Control Plasma, or Calibrator. This screen shows that the operator selected the Reagent category.   Do all required reagent parameters appear on a single screen? No. From the Reagent Information Setting screen, press Other Param to view additional reagent parameters to define: initial volume, alarm limit, interrupt limit and vial type. Reagent Information Setting Screen Learn about parameters on the Reagent Information Setting screen. Base ImageHotspotsText BlocksImage FileThe initial volume must be compatible with the vial type setting. The system uses the initial reagent volume to calculate the reagent volume remaining after each analysis.  One of six reagent categories appears here: Reagent, Factor Deficient Plasma, Buffer, Rinse, Control Plasma, or Calibrator. This screen shows that the operator selected the Reagent category.  The alarm limit is the volume that will trigger an alarm during reagent monitoring, alerting the operator that reagent is low. The alarm limit has a predefined default setting based on the vial type selected. The alarm limit should be set just a bit higher than the interrupt limit.  The interrupt limit is the volume that will trigger an analysis interruption due to insufficient reagent during reagent volume monitoring. The instrument continues to process tests not affected by the low-volume reagent if the alarm setting for reagent volume is set to Reagent Volume. The interrupt limit has a predefined default setting based on the vial type selected. The alarm limit should be set just a bit higher than the interrupt limit.  The system uses vial type data to calculate the volume of reagent remaining after each assay. All Sysmex® CA-1500 System reagents need the reagent vial type defined.Press the Other Param key to return to the previous Reagent Information Setting screen that displayed ID, Name, Lot #, Barcode ID, and Exp. Date parameters.Press Return to return to the Reagent Info submenu. Defining the reagent vial type during reagent management is very important. Why? The vial type selected automatically updates the alarm and interrupt limits, which the system uses to flag the operator when reagent volume is low or insufficient for testing.   Vial Types Learn about vial types. Base ImageHotspotsText BlocksImage FileUse 4-mL disposable cups for up to 4-mL reagent volumes.Use 2-mL disposable cups for smaller reagent volumes. To conserve reagent, QC reagents (which usually come in a 5-mL vial) are usually run in a 2-mL cup.GW5 vials hold up to 5-mL of reagent.Attached vials hold up to 50-mL of reagent.  Plastic push vials hold up to 10-mL of reagent and are used for buffers and rinse solutions.GW15 vials hold up to 15-mL of reagent.   Reagents are defined on the Reagent Information Setting screen—the area of software designed for customizing your instrument. To define reagents: From the Main Menu screen, press Settings, and then press Analysis Settings. From Analysis Settings, press Reagent Info. On the Reagent Information/Type Selection screen, press a reagent category key: Reagent, Factor Deficient Plasma, Buffer, Rinse, Control Plasma, or Calibrator. On the Reagent Information Setting screen, select the reagent that you want to define. Note: To return to the Reagent Information/Type Selection screen to select an alternative reagent category, press Select Type. To set parameters, select each parameter setting, press Change, enter parameter settings, and then press Enter or OK, as applicable. To save any additions or changes or edits, press Set in the confirmation screen. Defining Reagents Learn about the reagent information and type selection screen. Use the Reagent Information/Type Selection screen to select on of the six reagent categories that you need to define. For example, press Reagent to open the Reagent Information Setting screen, where you can define each reagent used on the Sysmex® CA-1500 System. Use the Reagent Information Settings screen to define each reagent. Press Select Type to return to the Reagent Information/Type Selection screen to select an alternative reagent category. When complete, select the X in the upper-right corner to close the window and continue. Reagent management tasks include defining NEW reagents. You have two options: You can add a reagent from a list of predefined reagents on the Select Reagent window, or You can add a new reagent manually, if the reagent name does not appear on the Select Reagent window. To define a new reagent: From the Main Menu screen, press Settings, press Analysis Settings, and then press Reagent Info. On the Reagent Information/Type Selection screen, press a reagent category key. On the Reagent Information Setting screen, in the ID column, select the row where you want to insert a new reagent, and then press Add. On the Select Reagent window that appears, do either of the following: To add a reagent from the list: Move the cursor to select a reagent, and then press OK. Both the reagent ID and reagent name will be updated. To add a reagent NOT on the list: Select Others, and then press OK. On the keypad that appears, enter the 4-digit reagent ID, and then press Enter. The updated reagent ID appears and the cursor automatically moves to the Name column. Note: To set non-Siemens reagents, use a unique 4-digit number. Press Change, enter the reagent name using the alphanumeric keypad, and then press Enter. The cursor automatically moves one column to the right. 5. To set the remaining parameters, select each parameter setting, press Change, enter parameter settings, and then press Enter or OK, as applicable. Define Reagents Learn how to update reagent information. 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