Sysmex® CA-1500 System Sample Processing I Online Training

Describe the Sysmex® CA-1500 System sampling process Describe three ways to register sample order information Identify key areas of the Work Load List screen Identify sample order information that the instrument needs to process samples List the steps to register samples using a Rack or STAT Sample Holder worklist   Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations. You have completed the Sysmex® CA-1500 System Sample Processing I online training course. Listed below are the key points that have been presented. Take time to review the material before you proceed to the final quiz. Describe the Sysmex® CA-1500 System sampling process Sample registration → sample dispensing→ sample plate well → reaction tube → incubation well → reagent dispensing → detector wells → measurement → Stored Data → reaction tube disposal. Identify sample order information that the instrument needs to process samples Before the instrument can process samples, the system needs three key pieces of information: the sample ID, the tests requested, and the instrument position where the sample is loaded. Identify key areas of the Work Load List screen Use the Work Load List screen to register sample order information and to monitor the sample processing status of samples on a Rack, STAT Sample Holder, or Reagent Holder worklist. Describe three ways to register sample order information You can register sample order information manually, semi-automatically, and automatically. Describe how to register samples using a Rack or STAT Sample Holder worklist Main Menu → Work List → Enter sample information→ Load sample → Start   Select Next to continue. Welcome to the Sysmex® CA-1500 System Sample Processing I Online Training course. Sample processing begins with receiving samples, and loading them onto the Sysmex® CA-1500 System for processing. Sample order information can be registered manually, semi-automatically, or automatically. There are three key pieces of information the system needs in order to process a patient sample. These three key pieces of information include: The sample ID The tests requested The instrument position where the sample is loaded There are also three places in the instrument where samples can be loaded: In the sample rack STAT sample rack Reagent holder Select Next to Continue. The Sysmex® CA-1500 System processes samples-- from registering sample order information to disposing the reaction tube. There are key steps during sample processing. The sample probe dispenses sample into a sample plate well. This step is bypassed if you are sampling in Micro mode. The sample probe aspirates sample from the sample plate well and dispenses it into a reaction tube. The catcher transports the reaction tube to an incubation well to warm the sample before reagents are added, and then to the reagent dispensing position. As the reagent probe dispenses reagents into the reaction tube, the catcher's vortex action mixes reagents. The catcher transports the reaction tube to a clotting or chromogenic/immunologic detector block well. Finally, the catcher transports the reaction tube to the tube trash box. Worklists Learn about worklists. Select the appropriate worklist for the type of sample you need to process: Use a Rack worklist to process routine samples, STAT samples, calibrators, or QC samples. Use a STAT Holder worklist to process STATs. Use a Reagent Holder worklist to process calibrators or QC samples. When complete, select the X in the upper-right corner to close the window and continue. Sample Processing Learn about sample processing. Slide NumberText BlocksCalloutsAudio ScriptImage File1Sample processing To begin sample processing: Register sample order information using the Work Load List screen Select Next to continue. Note: If audio does not automatically start, select the play arrow in the top left to begin.To begin sample processing, register sample order information using the Work Load List Screen. Select next to continue. 2Sample Processing: Aspiration Load samples into a sample rack: The sampler moves the sample rack to the aspiration position The sample probe aspirates sample then dispenses into a sample plate well Sample is dispensed into a reaction tube Select Next to continue.Once the samples are loaded into a sample rack and placed on the instrument, the sampler moves the sample rack in the right input tray to the aspiration position. The sample probe aspirates sample and dispenses it into a sample plate well. The sample probe then aspirates sample from a sample plate well and dispenses it into a reaction tube. Select next to continue. 3Sample Processing: Transport The Catcher: Transports the reaction tube to an incubation well Transports the reaction tube to a reagent dispense position Mixes reagents as they are being dispensed into the reaction tube Transports the reaction tube to a detector well Select Next to continue.After the sample is dispensed into a reaction tube, the catcher transports the reaction tube into an incubation well to incubate. After incubation, the catcher transports the reaction tube to the reagent dispensing position where the catcher's vortex action mixes the reagents as they are being dispensed by the reagent probe, into the reaction tubes. Depending on the test method, the catcher will then transport the reaction tube to a detector block with either clotting detector wells or chromogenic/immunologic detector wells. Select next to continue. 4Sample Processing: Detection Methods There are three types of testing methods: Clotting: the reaction is detected through light scatter Chromogenic: the reaction is detected through the change in transmitted light Immunologic: the reaction is detected through the change in transmitted light Select Next to continue.   In clotting detector wells, the coagulation reaction is detected through the change in scattered light. Each clotting detector well has its own light source--an LED at 660 nanometers. In chromogenic detector wells, the reaction is detected through the change in transmitted light. In immunologic detector wells, the reaction is also detected through the change in transmitted light. A halogen lamp provides the light source for both chromogenic and immunologic test methods. Select next to continue. 5Sample Processing: Results Once sample processing is complete: The instrument posts results to the Stored Data screen The catcher transports the reaction tube to the trash box   When complete, select the X in the upper-right corner to close the window and continue.Once sample processing is complete, the instrument will post results, which can be viewed on the Stored Data List screen. The catcher will also transport the used reaction tube into the tube trash box. When complete, select the X in the upper-right corner to close the window and continue. The Sysmex® CA-1500 System hemostasis tests are performed using patient plasma collected in sample tubes that contain anticoagulant that does not interfere with the following pathways to coagulation: Intrinsic Pathway: Coagulation pathway activated by a foreign surface and involves contact factors PK and HMWK, and XII, XI, IX, and VIII. Most congenital deficiencies involve factors in this pathway. Extrinsic Pathway: Normal physiologic coagulation follows this pathway, which is activated by tissue factor and also involves factor VII. Most of the factors depressed by oral anticoagulant drugs are involved in this pathway. Common Pathway: Coagulation pathway activated by either the VIIa/tissue factor complex from the extrinsic pathway, or the VIIIa/IXa complex from the intrinsic pathway. Factors involved in the common pathway include factors X, V, II and fibrinogen. To ensure sample quality, follow these collection tips: Collect sufficient sample volume to achieve a venous blood to anticoagulant ratio of 9:1. For PT tests, store plasma at 18-24ºC for up to 24 hours. For PTT tests, store plasma at 2-4ºC, or 18-26º C for up to 4 hours. Sample Collection & Preparation Learn about Sample Collection & Preparation. Anticoagulant 3.2% and 3.8% sodium citrate (designated by a light blue capped tube). Sample Cups 2-mL or 4-mL When using the VACUTAINER Plus Plastic Citrate Tube (HEMOGARD Closure) = 1.8 mL, sample cups cannot be used in a sample rack. Sample Tubes Outer diameter- 10-15 mm Length- 65 mm - 78 mm Note: Tubes with an inner diameter <9.4 mm cannot be used. VACUTAINER Plus Plastic Citrate Tube (HEMOGARD Closure) = 1.8 mL, 2.7 mL. When using these special tubes, the correct tube type must be selected in the tube type selection settings. When complete, select the X in the upper-right corner to close the window and continue. To process a sample, the following sample order information needs to be entered—or registered—into the system: Sample ID: Register up to 15 digits using numerals or hyphens. Tests requested: Register the test parameters that you need to run on the sample, such as PT, APTT, and Fbg. Sample position: As you select a position on the worklist to enter the sample ID, you are simultaneously designating sample position—the selected position on the worklist corresponds to one of the following sample locations on the instrument: Sample racks can be used to load routine samples, STAT samples, calibrators, and QC samples. STAT sample holders are used to load STATs, and reagent holders (reagent tray 2) are used to load calibrators and QC samples. To process a sample, the following sample order information needs to be entered—or registered—into the system. Note: Sample position refers to one of three locations in the instrument where you can load a sample that needs processing: sample rack, STAT sample holder, or reagent tray 2. Select Next to continue. To process samples on the Sysmex® CA-1500 System, use the Work Load List screen to enter—or register—sample order information onto a worklist. You have several registration options—it depends on your lab's host computer setup: You can manually register all order information onto the worklist. You can semi-automatically register order information—manually register the sample ID number onto the worklist, and download test requests from a host computer. You can automatically register order information—the barcode reader reads the sample ID and the host computer sends test requests. Select Next to continue.   Use the Work Load List screen to prepare a worklist, where you can register sample order information, as well as monitor sample processing status. The Work Load List screen displays the following sample order information that the instrument needs to process a sample: sample ID, tests requested, and sample position in the instrument. The Work Load List screen can display three different worklists—one for each position where you can load samples: in a rack, in a STAT sample holder, or in a reagent holder. The instrument will store up to 25 worklists. The instrument will overwrite the oldest worklist until the instrument is powered off, and then back on clearing all worklist data. To view a worklist: From the Main Menu screen, press Work List. The Work Load List screen appears and displays the Rack worklist. To view an alternative worklist, from any worklist, press the Rack, STAT Holder or Reagent Holder key. To view a previous worklist, select Prev. Identify Work Load List Learn about the Work Load List. There are different worklists that are utilized depending on the type of sample being processed. Worklists include: Rack worklist: to process routine samples, STAT samples, calibrators, or QC samples. STAT Holder worklist: to process STATs. Reagent Holder worklist: to process calibrators or QC samples. The name of the worklist is located on the top of each screen. The key of the worklist that is currently being displayed will appear inactive. When complete, select the X in the upper-right corner to close the window and continue. Work Load List Screen Learn to navigate the Work Load List. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90013491/sim/sim.CA1500_sampleprocessingI_Rackworklist_scaled.htmHTML5 File:/content/generator/Course_90013491/sim/index.htmlPDF File: The Rack worklist is used to process routine samples that are loaded into a sample rack. There are different modes the samples can be processed: Normal mode: The sample probe will dispense sample into a sample plate well. Micro mode: The sample probe will bypass the sample plate well and dispense sample directly into a reaction tube to conserve low-volume samples. Reflex mode: The instrument will automatically run another test method for a sample if one or more tests ordered for that sample yield results outside the reflex limits set by the laboratory. Repeat Function Learn about the repeat function. Using the Repeat Function: Samples can be registered to repeat the same test on multiple samples on a worklist. Pressing the Repeat key will the same test for multiple samples. Tests will appear in consecutive positions on the worklist after the initial sample that is selected for repeat. The instrument can also register sample ID numbers as consecutive numbers, if desired. To use the repeat function: Press the Up and Down arrow keys to move the cursor to the sample for which you want to repeat test parameters ordered for that sample. Enter the number of positions to repeat by pressing Repeat. Include the selected sample in your number. Press Enter: Registered tests appear in consecutive positions on the worklist after the initial sample that you selected.   When complete, select the X in the upper-right corner to close the box and continue. Rack Worklist Learn about the rack worklist. Base ImageHotspotsText BlocksImage FilePress a Profile key to order a pre-set profile of tests. Profiles are defined during instrument setup. You can create two test profiles.You can display two different test groups--Group 1 and Group 2. The worklist shown here displays tests defined for Group 1.-The six-digit rack ID for the current worklist appears here. -Each sample rack can hold up to 10 tubes in positions 01 through 10. -Register Sample ID numbers and hyphens (up to 15 characters). For QC samples, you can register QC01 through QC20.Test names (parameters) for the selected Group appear here. If a test name appeared backlit in red, there is insufficient reagent onboard to run this test for any sample. In this case, you must add more reagent.  If a test name is backlit in yellow, there is insufficient reagent onboard to run this test for the number of samples on the worklist with this test request. In this case, you must reduce the total number of orders on the worklist for this test, or add more reagent.Test keys appear for each test included in Group 1 or Group 2. Press a test key to order a test for a sample. Press the Other Tests key to display additional test keys included in a profile. The symbol on each key indicates the analysis status of the test for a selected sample. O = Test ordered __ = Test NOT orderedPress Stat Holder to view the STAT Sample Holder worklist so that you can register order information for STAT samples that you will load in the STAT sample holder. Each STAT sample holder can hold up to five samples--tubes or cups.Press Reagent Holder to view the Reagent Holder worklist so that you can register order information for calibrator or QC samples that you will load in positions D1 - D14 in reagent tray 2.Press Rack No. Entry to manually register a rack ID number.Press ID No. Entry to manually register a sample ID number. You can use up to nine digits.Press Repeat to register the sample analysis parameters in consecutive positions on the worklist. ID numbers (if desired) will be registered as consecutive numbers. This "copy and paste" feature allows for batch testing.Press Sample Specific to open the Sample Specific screen, where you can enter detailed settings for a sample, such as a re-dilution, re-analysis, dilution ratio, or dilution series of Multiple Dilution Analysis (MDA).Press Standard Curve to order a calibration curve.If the sample volume is minimal, press Micro to program a sample to run in Micro mode. The sample probe bypasses the sample plate well and dispenses sample directly into a reaction tube to conserve sample. The symbol O appears on the Micro key for a selected sample if Micro mode has been programmed for that sample. Note: Reflex testing is not available if Micro mode is programmed for a sample.Press Reflex to program a sample for reflex testing. If reflex test conditions have been set up, the instrument will automatically run another test method for a sample if one or more tests ordered for that sample yield results outside reflex limits set by the laboratory. The symbol O appears on the Reflex key for a selected sample if reflex testing has been programmed for that sample. Note: Micro mode is not available if reflex testing is programmed for a sample.Press Main Menu to return to the Main Menu screen.The O symbol indicates that the test has been ordered for the selected sample.Press Register More when manually entering multiple worklists.Press Prev to display the previous worklist.Press the Up arrow key to move the cursor up one line.Press the Down arrow key to move the cursor down one line.Press Next to display the next available worklist. Press this key whenever you want to "start fresh" with a new worklist.The Test Group number is indicated here.Flags indicate if detailed settings are programmed for a sample. Press the Sample Specific key to enter detailed settings for a sample. M = The sample will be run in Micro mode. R = Reflex testing has been programmed. C = Routine re-dilution, re-analysis, or dilution ratio settings have been changed for this sample.In the Analysis Progress area of the Worklist, sample rows appear backlit in colors and display symbols to indicate the analysis status of each sample for each test in the selected Group. Monitor this area to keep track of the sample processing status of samples on a worklist. O = Test ordered __= test NOT ordered Double circle = Test analysis in progress X = Test analysis NOT completed due to errors = No tests ordered  Backlit in blue = Tests ordered but analysis has not begun Backlit in Green = Test analysis in progress Backlit in Light blue = Test analysis completed The STAT Sample Holder worklist is used to process STAT samples that are loaded into the STAT sample holder. There can be up to five samples registered on the STAT Sample Holder worklist. Test Groups Learn about test groups. Test Groups are established during instrument setup using the Analysis Settings screen. Group parameters include test method, test position, reagent name, reagent lot number, reagent expiration, and reagent vial size. The instrument can have up to up two different test groups—Group 1 and Group 2. On the Consumables screen, press the Select Group button to toggle between Group 1 and Group 2. Group 1 can hold up to 15 test parameters. Group 2 is used if a lab is running more than 15 test parameters or if the lab wants to place a more esoteric test in Group 2.  When complete, select the X in the upper-right corner to close the window and continue. STAT Sample Holder Worklist Learn about worklists. Base ImageHotspotsText BlocksImage FilePressing the yellow STAT key will suspend the sample processing so that a STAT sample can be added or removed. STAT samples can be loaded into a sample rack (using a Rack Worklist) or the STAT sample holder (using the STAT sample Holder Worklist).The STAT sample holder can hold up to five samples in positions 01 through 05.An S will appear indicating if the sample is a STAT sample.Press Rack to view the Rack Worklist. Each sample rack can hold up to 10 samples-- tubes or cups.Select Reagent Holder to view the Reagent Holder Worklist to register order information for a calibrator or QC samples that will be loaded in positions D1-D14 in reagent tray 2.Select HC to query the Laboratory Information System (LIS) for sample order information if a STAT sample is placed in the STAT sample holder position.If a sample order is backlit in light blue, the test analysis is completed.If there is a — symbol on a test key this indicates that a test has not been ordered for the selected sample. The Reagent Holder worklist is used to process calibrators or QC samples that are loaded into reagent tray 2.   Reagent Holder Worklist Learn about the reagent holder worklist. Base ImageHotspotsText BlocksImage FileSelect Other Tests key to display additional test keys.If a test key name is backlit in red, there is insufficient reagent onboard to run this test for any sample.If a sample order appears backlit in green, the test analysis is in progress.Sample positions in reagent tray 2 appear hear. (D05, D06, D07 etc.)For calibrators, register sample ID numbers using numbers and hyphens (up to 15 characters). For QC samples, you can register QC01 through QC20.Select Standard Curve to order a calibration curve.Select Rack to view the Rack Worklist. Each sample rack can hold up to 10 samples--tubes or cups.Select STAT Holder to view the STAT Sample Holder worklist. Each STAT sample holder can hold up to five samples--tubes or cups. There are three ways to register sample order information into the system so that the Sysmex® CA-1500 System can process samples: Manual registration Semi-automatic registration Automatic registration Select Next to continue.   Samples can be manually processed by using the Work Load List screen to register sample ID information and test requests for each sample position on a Rack or STAT Sample Holder worklist. During sample processing, be sure to monitor sample status by observing the analysis indicators and symbols on the worklist. To process samples using manual registration: From the Main Menu screen, press Work List On the Work Load List screen, press Rack or. STAT Sample Holder. Press ID No. Entry. and then enter the sample ID. Order tests, and then press Enter. Load samples into assigned positions. For sample racks, place rack in the right input tray. Press Start. Manual Registration Learn how to manually order samples. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90013491/sim_CA1500_SampleProcessing1_ManualRegistration_scaled/sim_CA1500_SampleProcessing1_ManualRegistration_scaled.htmHTML5 File:/content/generator/Course_90013491/sim_CA1500_SampleProcessing1_ManualRegistration_HTML5_800x600/index.htmlPDF File: Samples can be processed semi-automatically if the Sysmex® CA-1500 System is connected to a host computer that uses bi-directional communication (Two-way data transmission where the host computer or Laboratory Information System [LIS] and the Sysmex® CA-1500 System send and receive communication through the data line in the form of a test request, control results, etc). To process samples via semi-automatic: From the Main Menu screen, select Work List. On the Work Load List screen, select Rack or STAT Holder. Select ID No. Entry. Enter the sample ID, and then select Enter. Load samples into assigned positions. For sample racks, place rack in the right input tray. Select Start. Select Next to continue.   Samples can be processed Automatically if utilizing the barcode-supplied sample IDs and if the Sysmex® CA-1500 System is connected to a host computer that uses bi-directional communication. No operator intervention is required. When the sample ID is read, the host inquiry takes place and the analysis parameters are automatically registered. Register sample ID information onto the worklist by reading barcode labels on tubes. Register test requests by downloading data from the host computer (LIS) to the Sysmex® CA-1500 System. As samples are processed, the Sysmex® CA-1500 System populates the worklist with sample order information. During sample processing, be sure to monitor sample status by observing the analysis indicators and symbols on the worklist.   To process samples automatically: From the Main Menu screen, select Work List On the Work Load List, select Rack or STAT Sample Holder. Load barcoded samples. For sample racks, place rack in the right input tray. Select Start and return to the Main Menu screen. Select Next to continue.