Sysmex® CA-500 Series System Reagent Setup Online Training

Welcome to the Sysmex® CA-500 Series Reagent Management online training course. In this course, you will learn about setup tasks to initially define reagent information to the system. NOTE: This course does not include audio. Upon successful completion of this course, you will be able to: List the initial reagent setup tasks performed by the TAS List daily reagent setup tasks Identify reagent positions in the analyzer Identify reagent vial types Locate reagent information This course does not include audio. Congratulations. You have completed the Sysmex® CA-500 Series Reagent Setup online training course. Listed below are the key points that have been presented. Take time to review the material before you proceed to the final quiz. List initial reagent setup tasks performed by the TAS. During instrument setup or as needed, your Siemens Healthcare Diagnostics Technical Applications Specialist (TAS) will set up test groups and set reagent holder positions and vial types to initially define reagent information to the system. List daily reagent setup tasks. Daily reagent setup tasks include verifying reagent information on the Standard Curve screen, loading reagents into their assigned reagent holder positions, and monitoring reagent volumes throughout sample processing. Identify reagent positions in the Sysmex® CA-500 series coagulation analyzer. The reagent rack provides four reagent holder positions to store reagents at 15° C, and six reagent holder positions to store reagents at room temperature. Two additional reagent holder positions provide room temperature storage for buffer and CA-Clean I. Identify Siemens Healthcare Diagnostics reagent vial types. Use 2-mL disposable cups for smaller reagent volumes, such as QC plasma. Use 4-mL disposable cups for up to 4-mL reagent volumes. GW5 vials hold up to 5-mL of reagent. Plastic push vials hold up to 10-mL of reagent and are typically used for buffers and rinse solutions. Locate reagent information using Package Insert Sheets, Application Sheets, and Tables of Analytical Values. The Package Insert Sheet for a specific reagent explains how to reconstitute the reagent, how much diluent to use, and how long closed reagent vials are stable. For PT reagent, the Package Insert Sheets also lists ISI values for calculating INR. The Application Sheet for a specific test lists the reagents needed to perform each test, reagent position and onboard stability of reconstituted reagents, QC products; test protocol settings, and calibration information, such as calibrator, dilutions sets, and number of replicates. The Table of Analytical Values lists assay values for calibrators, such as Standard Human Plasma (SHP). During instrument setup or as needed, your TAS will perform the following reagent setup tasks to initially define reagent information to the system: Set up test groups Set reagent holder positions and vial positions A test group is a pre-defined group of tests displayed on the worklist as you load samples and order tests. Your TAS will define three test group configurations to suit the needs of your laboratory. For example, Test Group 1 could include PT, APTT, Fbg, and D-Dimer.  Each reagent holder position must be assigned to a specific reagent and vial type so that the system knows which reagent to aspirate and which automatic probe height adjustment to implement when the probe aspirates reagent from varying vial sizes. Reagent Holder Positions Learn how to define reagent holder positions. Your TAS will define each reagent holder position and vial type for all the reagents needed to perform each test in each test group. For example, the TAS would typically define reagent holder positions shown here. PT APTT Fbg Thrombin CACl2 CA Clean I D-Dimer Accelerator D-Dimer Reagent Buffer (OVB or CA System Buffer) There are two Test Group Keys in the system software: From the Main Menu screen, press Test Group to select the test group to display on the worklist. The other Test Group key appears on the Analysis Settings screen and is used by the TAS to initially define the three test groups. On a daily basis, perform the following reagent setup tasks: Verify reagent information The Standard Curve screen displays reagent information currently used by the system. Before loading reagents, verify that the reagents, lot numbers, and expiration dates match the reagent information displayed on the Standard Curve screen to ensure that you are using the same reagents that the system uses to calculate final test results. To open the Standard Curve screen, from the Main Menu screen, press Standard Curve. On the Standard Curve screen, press Select Test to view the reagent information for the selected test.   Load reagents When you are ready to process samples, load the reagents that you will need into their assigned reagent holder positions. Refer to the Application Sheet for each test method, which lists the reagents, vial types, and reagent holder positions specified for each test method.   Monitor reagent volumes Throughout sample processing, reagent volumes can be monitored onboard the analyzer. This is an optional setup feature that your TAS can perform to automatically set the system to monitor the volumes remaining in each reagent vial. The reagent rack provides four reagent holder positions to store reagents at 15° C, and six reagent holder positions to store reagents at room temperature. Two additional reagent holder positions provide room temperature storage for buffer and CA-Clean I. Use adapters to allow varying-size containers (cups) to fit into larger-size reagent positions. Cooler Reagent Holders Room Temperature Reagent Holders Room Temperature Buffer and CA Clean I Holders The analyzer can use several types. Remember to consider any necessary dead volume for each vial type.  When the TAS defines the reagent vial type for each reagent then sets the system to automatically monitor reagent volumes, the system updates the initial volume setting and uses this setting to calculate the reagent volume remaining after each analysis. It is not necessary to enter the reagent volume because the analyzer has level sensors to recognize insufficient reagent. Cup 2 mL Cup 4 mL GW5 PV - 10 Click here to view frequently asked questions for locating Siemens Healthcare Diagnostics reagent information.