Sysmex® CA-1500 System Calibration Online Training

Welcome to the Sysmex® CA-1500 System Calibration Online Training course. In this course you will learn the key features of the Standard Curve screen. You’ll also get an overview of ways to perform system calibrations: Performing an automatic dilution & analysis Manually entering calibration data Reading calibration data from a floppy disk Select Next to continue. Upon successful completion of this course, you will be able to: Identify key areas of the Standard Curve screen Describe three ways to perform calibrations on the Sysmex® CA-1500 System List key points when evaluation a new standard curve Select Next to continue. Congratulations. You have completed the Sysmex® CA-1500 System Calibration online training course. Listed below are the key points that have been presented. Take time to review the material before you proceed to the final quiz. Identify key areas of the Standard Curve screen The Standard Curve screen displays calibration data used to prepare the standard curve, including assigned calibrator values, raw analysis data, and calculated values based on the standard curve. Describe three ways to perform calibrations on the Sysmex® CA-1500 System Use the Standard Curve screen to calibrate Sysmex® CA-1500 System test methods. You can perform an automatic dilution & analysis calibration, enter calibration data manually, or read calibration data from a floppy disk. Describe how to evaluate a new standard curve Use the Standard Curve screen to evaluate a new calibration by comparing new and current (old) standard curves so that you can determine whether or not to adopt or reject the new curve. Select Next to continue. Calibrations on the Sysmex® CA-1500 System are performed by running calibrators of known concentration and plotting the seconds or change of optical density (delta OD) against known values to create a standard curve. Coagulation tests are initially read optically and results are reported in either seconds or dOD. If another unit is required for reporting, such as mg/dL for fibrinogen assays, the calibration curve is used to convert seconds to mg/dL. Run a calibration for a parameter in the following situations: As specified in the Application Sheet of your reagent If you add a new reagent lot number for a test method or if a reagent lot number changes If you add a new test method to the instrument If you receive test method updates or software revisions that require a calibration If you have a problem with Quality Control If you are directed to calibrate by Technical Solutions Center personnel Refer to the Application Sheets included with each reagent lot for details about product specifications. The system WILL NOT use a reagent lot that has not been previously calibrated on the instrument or if the calibration has been rejected by the operator. Select Next to continue.   To perform calibrations on the Sysmex® CA-1500 System, use the Standard Curve screen, where you can select the parameter to be calibrated. Two common ways to perform calibrations on the Sysmex® CA-1500 System include the following: Calibrate via automatic dilution & analysis Calibrate by entering data manually You can also calibrate by restoring a standard curve for a specific test using data loaded from a floppy disk. Calibration methods have one important step in common—preparing the standard curve. Select Next to continue.   The Standard Curve screen serves as the hub of calibration activity. Use the Standard Curve screen to: Perform calibrations Display and store standard curve calibration data Evaluate a new calibration by comparing the new standard curve to the current standard curve Print a standard curve, or store standard curve data to a floppy disk There are several ways to perform calibrations. From the Standard Curve screen, press the Analysis Setting key to select the calibration mode: Auto Dil and Analyze or Manual Dil and Analyze. Standard Curve Screen Learn how to identify data on the Standard Curve screen. Base ImageHotspotsText BlocksImage FileOn the screen shown here, Validated appears because the standard curve displayed is the current curve being used by the system to measure Fibrinogen tests--the curve has been adopted. Note: If the curve has not been adopted Not validated will appear.The test method for the standard curve currently displayed appears here.On the screen shown here, Current appears because the standard curve displayed is the current curve being used by the system to measure Fibrinogen tests. Note: If the curve displayed is from a new calibration, New will appear.The vertical Y-axis shows analysis data in seconds or dOD.The horizontal X-axis shows percent activity or concentration.Standard curve statistics appear here.This area displays data used to determine the standard curve. The first column displays assigned calibrator values (activity percent or concentration). The second column displays raw analysis data (coagulation time or delta OD). The third column shows activity percent or concentration calculated for data points on the standard curve. Reagent lot information for the reagents and calibrator used in analysis must be listed in this area. On this screen you can see that the Fibrinogen calibration requires: Fbg- Siemens Diagnostics Thrombin Reagent SHP- (Standard Human Plasma) Calibrator OVB - Buffer/Diluent Standard Curve: When setting standard curves on the Sysmex® CA-1500 System, the system plots the calibrator's raw (sec or delta OD) vs. assigned calibrator values (percent activity or concentration) to prepare a standard curve.Press Select Tests to select another test to calibrate.Press Analysis Setting to set up the calibration process.Press Manual Entry to manually enter calibrator data, ISI values and PT Mean Normal values if not already set up for you.Press Update to adopt a new standard curve.Press More to display additional keys to perform other standard curve options, such as zooming in on the standard curve graph to enlarge the display or copy curve data to a floppy disk, or print a curve. Calibrations can be performed via automatic dilution & analysis, by manually entering the calibration data or by reading a calibration curve from an inserted floppy disk: Calibrate via automatic dilution & analysis The instrument performs predefined dilutions on a single calibrator, and then uses the analysis results to create a new standard curve. Calibrate by manually entering data Manually enter calibration assigned values and raw analysis results—the instrument uses the entered data to calculate a new standard curve. Calibrate via floppy disk The instrument reads the standard curve for the selected test from data saved to a floppy disk. Note: Be sure to refer to your Application Sheet when performing a new calibration to ensure that you have the most up-to-date information. Select Next to continue. When you perform an automatic dilution & analysis calibration, the instrument dilutes the calibrator and uses the analysis result from each dilution to prepare a standard curve. Follow three easy steps to perform an automatic dilution & analysis calibration. To perform an automatic dilution & analysis calibration: Set up reagents: Use the Consumables screen to load required reagents onboard the instrument. Prepare the calibration: Use the Standard Curve screen to select the test you want to calibrate, select the calibration method, and verify calibration analysis settings. Initiate the calibration: Use the Work Load List screen to register the calibration order. Use the Standard Curve Parameter Settings screen to order the calibration. Return to the Work Load List screen to start the calibration. Calibration via Automatic Dilution & Analysis Learn about key points for an automatic dilution & analysis. Remember the following key points when performing an automatic dilution & analysis: First, set up reagents using the Consumables screen. Then, prepare the calibration using the Standard Curve screen. When you're ready to initiate the calibration, register the calibration using the Work Load List screen and then order the calibration using the Standard Curve Parameter Settings screen. Then, return to the Work Load List screen to start the calibration. When complete, select the X in the upper-right hand corner to close the window and continue. Calibration Learn how to program a calibration. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90013626/sim_CA1500_Calibration_AutoDill1_2/sim_CA1500_Calibration_AutoDill1_2.htmHTML5 File:/content/generator/Course_90013626/sim_CA1500_Calibration_AutoDill_HTML5_800x600/index.htmlPDF File: You can also calibrate a test by manually entering calibrator values and corresponding raw data from a current analysis—the instrument uses the entered data to calculate a new standard curve for the selected test. For example, suppose that data was lost, and only a paper copy of the curve was saved. Now, you need to manually enter calibration data. To manually enter calibration data: From the Main Menu screen, select Standard Curve. On the Standard Curve screen, select the test to calibrate. Select Manual Entry, and then manually enter calibration data. To save the calibration, press Update twice. Select Main Menu to return to the Main Menu screen. Note: When performing an automatic dilution & analysis calibration, the instrument performs predefined dilutions on a single calibrator, and then uses the analysis results from each dilution to create the points on the standard curve. Select Next to continue. You can also restore a standard curve for a specific test by loading data from a floppy disk. The instrument reads the standard curve for the selected test from the files saved on the disk. This disk is similar to an archive file. To read calibration data from a floppy disk: From the Main Menu screen, press Standard Curve. From the Standard Curve screen, select the test to calibrate. Select the More key, then Output/Input, and then select Read from FD. Insert the floppy disk with the saved data into the disk drive. Note: You can also use the Standard Curve screen to output standard curve data to a floppy disk and a graphic printer. It is good practice to document your standard curve data in both hard copy (printed) and soft copy (floppy disk) formats—one document backs up the other. Select Next to continue. After completing a calibration, use the Standard Curve screen to evaluate the new standard curve so that you can decide whether or not to adopt or reject the new curve. Your evaluation criteria should include the following:  From the Standard Curve screen, compare the new standard curve to the current (old) standard curve. From the Standard Curve screen, compare raw data from the new standard curve to raw data from the current (old) standard curve. From either the Stored Data List screen or the QC screen, evaluate QC results that you ran following the calibration. During your calibration review, it's helpful to display both new and old standard curves on the Standard Curve screen simultaneously—the new curve appears as a solid line and the current (old) curve appears as a dotted line. On the Standard Curve screen, press Change Display to toggle views between new, old, or simultaneous standard curve displays. Standard Curve Errors & Warnings Learn about standard curve errors & warnings. A Standard Curve Error will appear if curve data does not increase constantly in relation to percent activity or concentration, if data does not decrease constantly in relation to percent activity or concentration, if only one point on the curve is set, or if one point is appreciably out from the curve. A Standard Curve Warning will appear if analysis results from a curve are flagged with the following: Coagulation Curve Error, Aged Sample, Slight Coagulation, Temperature or Early Reaction ERR codes. The standard curve can be accepted according to your laboratory procedure. When complete, select the X in the upper-right hand corner to close the window and continue.