Sysmex® CS-5100 System Calibration Online Training

Welcome to the Sysmex® CS-5100 System Calibration Online Training course. The Sysmex® CS-5100 System software: Manages multiple reagent lots for each assay Provides multiple methods of calibration data entry Enables comparison of calibration curves Sysmex® is a trademark of Sysmex Corp. Select Next to continue. Image depth may be adjusted by rotating the depth knob on the control panel. The  Image depth is displayed on the image screen and will be captured and stored. The range of depth settings will vary based on transducer that is selected.  Describe how to enter reagent lot information Describe how to evaluate calibration curve results Identify information on Application Sheets Identify the steps to order a calibration List the steps to enter the ISI and normal values for the PT assay Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations.  You have completed the Sysmex® CS-5100 System Calibration Online Training course.  Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz.  Identify information on Application sheets Application Sheets: Documents that have instrument and reagent specific information for assays. List materials required, on-board stability times, and interfering substances. Describe how to enter reagent lot information Activity Software Screen Instructions Enter Reagent Lot Data - Manual Reagent Lot Master Enter lot number in the Lot information window Select correct expiration date Select Add Enter Reagent Lot Data - 2D Barcode Reagent Lot Master Select Import Select the Barcode import method Scan the barcode Select Save   Explain how to perform a calibration Activity Software screen Instructions Order new calibration Order screen Select Switch Order Select Holder Calib. Curve Order Select the assay button Select Change Highlight lot Select OK Select the lot of standard or calibrator Manually enter target values if the 2D barcode was not used Select OK   Describe how to evaluate calibration curves Activity Software screen Instructions View and Validate the calibration curve Calibration Curve screen Select Change Select the assay Select Validate Compare Two Curves Calibration screen Display current curve Select Detailed Display Select Select Compared Calib. Curve Select another curve from list of available curves for the same assay Select Load Both curves will appear on the graph. Print Curve Calibration screen Display selected curve Select Print to start the print out Save Backup database Start Menu        1. Close IPU software        2. Select Start menu        3. Select All Programs        4. Select CS-Tools        5. Select Backup        6. Enter date for subfolder        7. Select Options        8. Select OK        9. Select OK      10. Perform Restart   Explain how to enter ISI and normal values for PT assay Activity Software screen Instructions Enter PT lot data into Reagent Lot Master Reagent Lot Master       Select Import Select Barcode Scan PT reagent 2D barcode Select Save Manual entry of ISI and Normal Calibration screen Select Change Select the PT assay Select the lot number Select Edit Select Lot Master to import the ISI Enter the Normal value Select OK Select Validate Select Next to continue.  4D control.  Depressing the 4D knob activates full volume imaging.  Adjust 4D gain by rotating the 4D control clockwise. The Sysmex® CS-5100 system can store: Up to 10 reagent lots for each calibrated assay Up to 10 curves for each reagent lot Select Next to continue.    Select Next to continueSelect Next to continueSelect Next to continueSeeleSelslsl    New for DEFINITION Systems with FAST CARE CARE kV adapts voltage AND effective mAs to the patient’s size and examination type based on stored reference factors for mAs and kV.  CARE kV enables optimized dose while providing optimized image quality. Failure to achieve isocenter potentially impacts accurate CARE kV selection.  Suggested parameters are displayed after acquiring the topogram and pre-monitoring image (when bolus tracking is utilized in a protocol).  When isocenter is markedly off but corrected with repositioning, repeat the topogram to provide the software with the most up to date attenuation profiles and dose calculations. Entry of Target Values with 2D Barcode Learn how to enter calibrator lot data using the 2D barcode Checklist TitleChecklist TypeChecklist ContentSelect Reagent Lot MasterHTML Select the Reagent Lot Master icon in the Settings menu screen. Select each checkbox to learn more about entering calibrator lot data using a 2D barcode. Select ImportHTML Select the Import button in the Reagent Lot Master window to begin the barcode read process.  Select the Barcode import methodHTML Select the Barcode import method.  Scan the barcodeHTML The Barcode Reader is equipped with an LED light.  To read the barcode, center the LED light over the barcode.  View and save the dataHTML The scanned data displays in the Barcode Reading Result window. Press the Save button to save the data to the system. When complete, select the X in the upper-right corner to close the window and continue. Enter Reagent Lot Data Manually Enter reagent lot data manually Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90006828/sim_CS51_ManuallyEnteringRgtLot_800x600/sim_CS51_ManuallyEnteringRgtLot_800x600.htmHTML5 File:/content/generator/Course_90006828/6_sim_CS51_ManuallyEnteringRgtLot__HTML5_800x600/index.htmlPDF File: Reagent and calibrator lot data can be entered in the Reagent Lot Master software screen by: Scanning a 2D barcode Manual entry MultiHertz allows routine use of higher frequencies with better penetration.  4 selectable frequencies are available on all transducers.  Try to start with the highest frequency and work your way down as penetration is needed.  Your 2-D frequency can be different from your color Doppler frequency.  Set your 2-D frequency, then select your preferred color Doppler frequency when you enter color Doppler.   At higher frequencies, you will have the best detail resolution and tightest pixel size, but the trade off will be in penetration.  So, if depth is important, select a lower frequency.. Use the multi-hertz toggle to cycle through available frequencies.  Toggle up to increase frequency, toggle down to decrease frequency.   Multiple imaging frequencies are available in 2D imaging including fundamental frequencies and native tissue harmonic frequencies.  Native tissue harmonics imaging frequency will be preceded by the letter H. The frequency range will be dependent upon the active transducer.   Delta – Adjust the delta to increase or decrease the level of differential echo levels. This increases or decreases the contrast within the image. Delta takes two structures of like echogenecity and enhances the difference between them. Please Click on the Pop up to view a Delta Run Video Reagent Table loading: Verify Reagent Table LED is green Open Reagent Table cover Load reagent- A or D rack Load calibrator- C rack The barcode scanner reads the vial barcode Diluent Table loading: Verify Diluent Table LED is green Open Diluent Table cover Load buffer onto rack The barcode scanner reads the vial barcode Loading Reagents, and Buffer Learn how to load reagents, and buffer Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90006828/sim_CS51_LoadingReagentsBuffer_750x600/sim_CS51_LoadingReagentsBuffer_750x600.htmHTML5 File:/content/generator/Course_90006828/5_sim_CS51_LoadingReagentsBuffer_HTML5_750x600/index.htmlPDF File: Loading calibrators using the C rack Learn how to load calibrators using the C rack Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90006828/sim_CS51_LoadingCalibrator_750x600/sim_CS51_LoadingCalibrator_750x600.htmHTML5 File:/content/generator/Course_90006828/4_sim_CS51_LoadingCalibrator_HTML5_750x600/index.htmlPDF File: This is a list of all of the parameters that can be saved in an image preset. You can see here that each mode has different things that can be saved to a preset that will allow for quick and easy optimization of the image. Ordering a Calibration: Access the Order screen. Select the assay to calibrate. Select the lot numbers. Press Start. Ordering the Calibration Learn more about ordering a calibration curve Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90006828/sim_CS51_OrderingCalibration_750x600/sim_CS51_OrderingCalibration_750x600.htmHTML5 File:/content/generator/Course_90006828/7_sim_CS51_OrderingCalibration_HTML5_750x600/index.htmlPDF File: You can create an optimized configuration of image parameter settings that is specific to the active exam, transducer, mode, and submode. The completed curve can be: Viewed on the Calibration Curve screen Compared to a previous curve Validated Printed Saved in database backup Evaluating and Validating the Calibration Learn more about viewing, validating and printing the calibration Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90006828/sim_CS51_ViewCompareCurve_750x600/sim_CS51_ViewCompareCurve_750x600.htmHTML5 File:/content/generator/Course_90006828/8_sim_CS51_ViewCompareCurve_HTML5_750x600/index.htmlPDF File: Calibration Screen Learn more about the calibration screen. Base ImageHotspotsText BlocksImage File Change key   This key is used to view another assay group and reagent lot. Calibration curve Display Area: Analysis values Calibration curve Calibration expiration date Calibration Curve Display Parameter Area Updated with date User that validated assay ID number Calibration Curve Display Parameter Area Status: Validated Assay Group name Reagent lot number Save a Copy of a Calibration Learn more about how to save a copy of the database Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90006828/sim_CS51_Backup1_750x600/sim_CS51_Backup_750x600.htmHTML5 File:/content/generator/Course_90006828/1_sim_CS51_Backup_HTML5_750x600/index.htmlPDF File: You can capture an optimized configuration of imaging parameter settings for a specific transducer by saving a user-defined Exam Preset. On the patient registration page choose exam preset under exam information Choose desired exam preset from pull down list To enter PT ISI and normal: Enter PT lot data by scanning 2D barcode into Reagent Lot Master Enter ISI and normal into PT Calibration screen Enter PT ISI using 2D barcode reader Learn more about entering PT lot data with 2D barcode reader Checklist TitleChecklist TypeChecklist ContentSelect Reagent Lot MasterHTML Select Reagent Lot Master to enter the reagent information. Select each check box to learn more about entering PT lot data using the 2D barcode. Select ImportHTML   Select the Import button on the operation panel.  Select BarcodeHTML   Select the Barcode import method.  Scan 2D BarcodeHTML   Scan the 2D barcode of the PT reagent.  View/Save the DataHTML The scanned data displays in the window. Press the Save button to save the information to the system. When complete, select the X in the upper-right corner to close the window and continue. Enter the PT ISI and Normal Values Enter the PT ISI and normal values into the calibration screen Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90006828/sim_CS51_EnterISINormal_750x600/sim_CS51_EnterISINormal_750x600.htmHTML5 File:/content/generator/Course_90006828/3_sim_CS51_EnterISINormal_HTML5_750x600/index.htmlPDF File: We are going to start with how to customize your measurements. In the left hand corner of the screen, click on the wrench icon( user configuration). From the user configuration menu,  choose the measurement tab. Select the measurement menu. Select the Measurement Menu Configuration blind (yellow arrow).  By blinds we mean the blue blinds, the dark blue tabs across the top of the configuration page. The Sysmex® CS-5100 System Application Sheets provide: Assay and reagent specific information List of materials required Reagent on-board positions Reagent on-board stability time Assay performance characteristics Application Sheet Learn more about application sheets Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90006828/sim_CS51_Cal_ApplicationSheet_800x600/sim_CS51_Cal_ApplicationSheet_800x600.htmHTML5 File:/content/generator/Course_90006828/2_sim_CS51_ApplicationSheet_HTML5_800x600/index.htmlPDF File: