Sysmex UF-1000i™ Urine Cell Analyzer System Overview Online Training

Welcome to the Sysmex UF-1000i System Overview Online Training course. The Sysmex UF-1000i system is a fully automated urine particle analyzer that uses flow cytometry to analyze elements in urine. Select Next to continue. Identify the location of the hardware components and their function Identify the methodology used to analyze the formed elements Navigate the software Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations.  You have completed the Sysmex UF-1000i System online training course.  Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz.    Identify the location of the hardware components and their function Main Unit components include: The front cover which is kept closed during normal operation of the system. LED Bar to indicate the status of the system. Manual Start Switch which starts an analysis in the manual mode and is used to bring the system to a ready status. Start Up Switch that initiates communication between the main unit and the IPU. The Aspiration Pipette which aspirates the urine sample. The sample filter that removes large particles from a sample. The sample rotor valve that aliquots the aspirated sample. The reaction units where the dilution and staining of the sample occurs. The vacuum trap chamber that prevents fluid from flowing into the compressor during an instrument failure. The Bellows which is used to adjust vacuum and the Relief Valve which is used to adjust pressure. The sheath syringe that introduces the stained sample to the flow cell. The five reagents: UFll SHEATH, UFll PACK SED, UFll PACK BAC, UFll SEARCH SED, UFll SEARCH BAC The detection unit which contains the optical assembly that includes a laser and flow cell. Information Processing Unit (IPU) : The IPU is the computer for processing data. Connected to the computer are the keyboard, mouse and hand held barcode reader and external drive. Navigate the software The UF-1000i instrument is controlled by the IPU. Navigate the software using function keys or by selecting icons on the main menu or tool bar. IPU logon usernames and passwords are user defined. Identify the methodology used to analyze the formed elements The Sysmex UF-1000i system provides enumerated results for five main parameters including RBC, WBC, Epithelial Cells (EC), Casts and Bacteria. Urine is aspirated, aliquoted and then mixed with a diluent and a fluorescent stain in a heated incubation chamber. Forward light scatter, Lateral light scatter and Fluorescence are detected as the cells surrounded by the sheath reagent pass through the laser illuminated flow cell. The instrument discriminates and classifies the formed elements into meaningful results. Each laboratory must establish their own review criteria for managing the results. Select Next to continue.   The major components of the system include: Main Unit Information Processing Unit (IPU) Main Unit Components Learn about the Main Unit components. Slide NumberText BlocksCalloutsAudio ScriptImage File1 Front Cover   The components of the Front Cover include: The LED Bar which indicates the status of the system The Manual Start Switch that starts an analysis in the manual mode and is used to bring the system to a ready status The Start Up Switch which initiates communication between the main unit and the IPU Select Next to continue. Note: If audio does not automatically start, select the play arrow in the top left to begin.Select each number to review the corresponding text.CalloutsLED Manual Start Switch Start Up Switch The front cover is kept closed during normal operation of the system. The LED Bar indicates the status of the system. Solid green is Ready, flashing green is sleep mode, orange is in operation, and red is not ready or in an error state. Select the Manual Start Switch to activate the pipette for an analysis in the manual mode and to bring the system to a ready status. The Start Up Switch initiates communication between the main unit and the IPU The Main power switch is located on the lower right corner of the front cover.2 Aspiration and Mixing Pipette   The Aspiration and Mixing Pipette: Aspirates the urine samples Mixes the samples when using the Track system   Select Next to continue.    The Aspiration Pipette aspirates the urine sample in both modes of sample aspiration. When running samples on the track the pipette also mixes the sample before aspirating.3 Rinse Cup The pipette is rinsed in the rinse cup with the UFll SHEATH reagent. Select Next to continue.   The pipette is rinsed in between each sample in the rinse cup with the UF Sheath reagent. 4 Sample Filter The Sample Filter removes large particles from a sample as the sample is aspirated. Select Next to continue. The sample filter removes large particles like cotton threads from a sample as the sample is aspirated.5 SRV The Sample Rotor Valve aliquots the aspirated sample. Select Next to continue. The sample rotor valve aliquots the aspirated sample for sediment and bacterial analysis. The loop on the left is for the sediment and the one on the right for bacteria.6 There are two Reaction Units: Sediment (SED) Bacteria (BAC) The reaction units are where the dilution and staining of the sample occurs. Select Next to continue. There are two reaction units, one for sediment and one for bacteria. The sample mixes with the diluent (the UF two Pack SED or Pack Bac reagents) and stain (the UF two Search SED and Search Bac reagents) in these heated chambers. Here, each sample aliquot is combined with the diluent and the stain and also heated and mixed prior to analysis.7 Vacuum Trap Chamber The Vacuum Trap Chamber prevents fluid from entering into the compressor. Select Next to continue. The vacuum trap chamber prevents fluid from flowing into the compressor during an instrument failure. As fluid enters the cup the ball inside the cup rises and blocks the path to the compressor.8 Bellows Vacuum Regulator The regulator is used to adjust the vacuum. Select Next to continue. The bellows vacuum regulator is used to adjust vacuum to minus 0.053 megapascals. The sensor screen in the IPU is viewed when making this adjustment. 9 Pressure Relief Valve The valve is used to adjust the pressure. Select Next to continue.   The relief valve is used to adjust pressure to 0.05 megapascals. The sensor screen in the IPU is viewed when making this adjustment.10 Sheath Syringe The Sheath Syringe introduces the stained sample to the flow cell. Select Next to continue. The sheath syringe introduces the stained sample to the flow cell. 11 UFll SEARCH SED Stain reagent for the sediment channel UFll SEARCH BAC Stain reagent for the bacteria channel Select Next to continue. The UF two Search SED reagent is held in a storage container. This reagent stains the sample in the sediment channel. The UF two Search Bac reagent is held in a storage container and is used to stain the sample in the bacteria channel.12 UFII PACK SED Reagent used to dilute the sample in the sediment channel UFII PACK BAC Reagent used to dilute the sample in the bacteria channel Select Next to continue.   The UF two pack SED is the diluent used to prepare the sample in the sediment channel while the UF two pack BAC prepares the sample in the bacteria channel. 13 UFII SHEATH Reagent used for laminar flow and all rinse functions including the pipette wash. Select Next to continue. In the flow cell, sheath fluid forms a liquid tunnel that cells pass through without mixing. The sheath is also used to rinse the system. 14 Detection Unit The detection unit contains the optical assembly which includes a laser and flow cell.   When complete, select the X in the upper-right corner to close the window and continue.Callouts Side (Lateral) Fluorescence Sensor Side (Lateral) Scatter Sensor Forward Scatter Sensor Flow Cell Laser Diode The detection unit contains the optical assembly which includes a laser and flow cell. Sample is forced through the flow cell surrounded by the sheath reagent. Forward scattered light, lateral scattered light and lateral fluorescent light measurements are detected from the sample and are then converted into meaningful results. IPU Components Learn about the IPU. Base ImageHotspotsText BlocksImage File Computer   Keyboard Mouse Handheld Barcode Reader Power Button Several new performance improvements have been included in the RAPIDComm system version 6.0 software. These include multiple host connections, time synchronization with the CLINITEK Status Connect System, automatic resend of failed result transmission, audit trail enhancements and automatic backup improvements. The Information Processing Unit (IPU) is the computer where the results from the main unit are sent. The Sysmex UF-1000i instrument is controlled by the IPU. Navigate the software using function keys or by selecting icons on the main menu or tool bar. Key Areas of the IPU Learn about the key areas of the IPU. Slide NumberText BlocksCalloutsAudio ScriptImage File1 Menu Bar Below the title bar is the Menu bar. Select a menu to view the items available in the drop down list. Select Next to continue.   Note: If audio does not automatically start, select the play arrow in the top left to begin.Below the title bar is the Menu bar. Software navigation includes multiple options including selecting items here from the drop down list under each menu. Select a menu to view the items available in the drop down list. 2 Tool Bar The Tool bar displays graphical buttons for specific functions. Select Next to continue. The Tool bar displays graphical buttons for specific functions. Select a button or the associated function key which is displayed in the button to navigate to that particular item. The Menu button or F4 returns you to the main screen.3 Tab View The main area displays icons for navigating to the IPU software screens.  Select Next to continue. The main area displays icons for navigating to IPU software screens. The icons that appear on this screen are customized using the Design toolbar button.4 Status Bar The left side of the status bar displays the condition of the main unit, the level of reagent remaining, an L-J if QC is being run, error messages and the sample ID.  An error message will override the above information.   On the right side the HC icon is green when communicating with WAM and red when there is a failure to communicate with WAM.  Select Next to continue. The left side of the status bar displays the condition of the main unit which is either green for ready, blinking green for standby, orange for busy or red for an error condition. Next to the condition of the main unit is an indicator for each reagent. The level of green indicates the amount of reagent remaining. When a control is run a blue L-J or a pink L-J will appear. Error messages and sample ID. Any Error message will take precedence over the status information. On the right side the HC icon indicates green when communicating with WAM and red when communication fails.5 Logon IPU logon usernames and passwords are user defined.   When complete, select the X in the upper-right corner to close the window and continue.IPU logon usernames and passwords are user defined and are customized when the system is installed. Navigating the Software Learn about navigating the software. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90006988/SIM_UF1Ki_SoftwareNavigation_800x600/SIM_UF1Ki_SoftwareNavigation_800x600.htmHTML5 File:/content/generator/Course_90006988/SIM_UF1Ki_SoftwareNavigation_HTML5_800x600/index.htmlPDF File: A number of new features have been included in RAPIDComm version 5.0 related to the blood gas, urinalysis and diabetes systems.  Select the links on this page to learn about these enhancements. The Sysmex UF-1000i system provides enumerated results for five main parameters including: RBC WBC Epithelial Cells (EC) Casts Bacteria Methodology Learn about the methodology used. Slide NumberText BlocksCalloutsAudio ScriptImage File1 Sample Flow Urine is aspirated, aliquoted and then mixed with a diluent and a fluorescent stain in a heated incubation chamber. Sediment chamber Heated to 35°C Sample mixes with UFll PACK SED and UFll SEARCH SED Bacteria chamber Heated to 42°C Sample mixes with UFll PACK BAC and UFll SEARCH BAC   Select Next to continue.     Note: If audio does not automatically start, select the play arrow in the top left to begin.Callouts Sediment Diluent Sediment Stain Sediment Chamber Bacteria Diluent Bacteria Stain Bacteria Chamber Urine is aspirated and then mixed with diluent and a fluorescent stain in a heated incubation chamber. In the sediment chamber heat in combination with the diluent dissolves the amorphous crystals and maintains the shape of the RBC's. The polymethine dye stains parts of the nucleus. In the bacteria chamber the higher temperature suppresses non-specific staining of particles other than bacteria.2 Flow Cytometry   Flow Cytometry is used to analyze formed elements by measuring: Forward light scatter Lateral light scatter Lateral fluorescent Light Select Next to continue.   Flow cytometry is used to analyze the formed elements. Forward light scatter, Lateral light scatter and Fluorescence are detected as the cells surrounded by the sheath reagent pass through the laser illuminated flow cell. Urine cells are classified by enumerating the characteristics of these measurements. 3 Analysis Parameters              RBC (Red Blood Cells)                WBC (White Blood Cells)             EC (Epithelial Cells)                      CAST                                            BACT (Bacteria)   Select Next to continue.   The instrument discriminates and classifies the formed elements into meaningful results. Red blood cells, white blood cells, epithelial cells, casts and bacteria are reported as numerical results on the Browser screen.4 Flagged Parameters X'TAL (Crystals) YLC (Yeast-like Cells) SRC (Small Round Cells) Path CAST (Pathological casts) SPERM MUCUS When complete, select the X in the upper-right corner to close the window and continue.Crystals, yeast-like cells, small round cells, pathological casts, sperm and mucus display with a plus sign if present. Each laboratory must establish their own review criteria for managing the results.