Syva® Viva-ProE™ System Calibration and Control Online Training

Welcome to the Syva® Viva-ProE™ System Calibration and Control Online Training course. During this training, you will learn about the calibration and control requirements for the Viva-ProE including how to request and run calibrations and controls on the system, and how to evaluate results. Select Next to continue. Upon successful completion of this course, you will be able to: Describe the steps to perform calibration and quality control (QC) testing Review calibration and QC results Select Next to continue. Congratulations. You have completed the Syva® Viva-ProE™ online training course. Listed below are the key points that have been presented. Take time to review the material before you proceed to the final quiz. Calibration and Control Testing Qualitative testing (e.g. for DAT) requires a one point calibration Quantitative assays (e.g. validity) require at least a two point calibration System calibration is required under the following conditions: Control results for an assay do not meet or exceed expected separations and/or control ranges Control results have shifted compared to previous control results A new lot of reagent is opened Major maintenance has been performed Troubleshooting An interval determined by laboratory procedures Control testing is run daily and also whenever a calibration is performed Additional frequency for control testing is determined by laboratory procedures Qualitative controls are Level 0 and Level 5 Quantitative Controls are UTAK. The level chosen is test-specific To run Calibrators: Print the previous calibration from the Results screen Order and assign calibrators in the Calibration screen Load Calibrators onto the Sample Rotor Start the run To run Controls: Order and assign from the Controls screen Load Controls onto the Sample Rotor Start the run  Review Results Use the Results screen to evaluate the results of the calibration and control testing Evaluate Calibration: Compare the current calibration to the previous one Curves should be relatively similar Accept or reject results (Supervisor or Administrator function) Evaluate Controls: Qualitative – Level 0, negative and Level 5, positive Quantitative – Compare numeric value for each level with expected results. In range, positive and out of range, negative Use the Unload icon from the Home screen to unload calibrator and control samples Use the QC screen to evaluate the performance of Controls over time Up to 100 control testing results displayed View in graph or table format Review any out of range controls that were manually accepted Add comments and exclude control points, if necessary Select Next to continue. Qualitative testing (e.g. for DAT) requires a one point calibration. Quantitative assays (e.g. validity) require at least a two point calibration. System calibration is required under the following conditions: Control results for an assay do not meet or exceed expected separations or control ranges Control results have shifted compared to previous control results A new lot of reagent is opened Major maintenance has been performed Troubleshooting An interval determined by laboratory procedures Control testing can be run at the same time as the calibration. It is required to be run daily and also whenever a calibration is performed. Additional frequency is determined by laboratory procedures. Perform a Calibration Learn about performing calibrations Checklist TitleChecklist TypeChecklist ContentReview Status of Existing CalibrationsHTML From the Home screen, select Calibration > Review. View the test icons. Select each checkbox to learn more about performing calibration testing. Print Previous Calibration ResultsHTML From the Home screen, select Results > Calibration and Controls Select the test to review, then select Print Assign Calibrators to RotorHTML From the Home screen, select Calibration > Review. Select the desired calibrator(s) then select Accept to go to the Load Calibration screen. Select Load for each calibrator to assign it to a location on the Sample Rotor. Prepare and Load CalibratorsHTML Add the appropriate calibrator level to the sample cups and then load the sample cups into the assigned locations on the Sample Rotor. Lower the glass lid once all calibrators have been added. Start the Calibration RunHTML Select the Process icon then select Yes to start the run. If the internal barcode scanner is on, select Scan prior to selecting Process. When complete, select the X in the upper-right corner to close the window and continue. Perform Control Testing Learn about performing control testing Checklist TitleChecklist TypeChecklist ContentAssign Controls to the RotorHTML From the Home screen, select the Control icon. For each required control level, select the control to highlight it and then select Load. Level 0 (Negative) and Level 5 (Positive) are required to validate the stored calibration curve for DAT. UTAK controls are required to validate the stored calibration curve for Validity tests. Select each checkbox to learn more about performing control testing. Prepare and Load ControlsHTML Add the appropriate control level to the sample cups and then then load the sample cups into the assigned locations on the Sample Rotor. Lower the glass lid once all controls have been added. Start the runHTML Select the Process icon, then select Yes to start the run. If the internal barcode scanner is on, select Scan prior to selecting Process. When complete, select the X in the upper-right corner to close the window and continue. To evaluate calibration: Compare the current calibration to the previous calibration curve Accept or reject (Supervisor or Administrator function) Use control results to validate the calibration curve To evaluate controls: For DAT (qualitative), results will be negative or positive based on expected separation values For Validity (quantitative), results will be a numeric value that should fall within a defined range The Quality Control screen can be used to compare up to 100 control values and evaluate system performance over time.   Evaluate Results Learn about evaluating calibration and control results Checklist TitleChecklist TypeChecklist ContentEvaluate Calibration ResultsSimulationInstructions:Evaluating calibration results for T50Flash File:/content/generator/Course_90012383/Sim_ProE_EvaluatecCalSim01_9/Sim_ProE_EvaluatecCalSim01_9.swfHTML5 File:/content/generator/Course_90012383/Sim_ProE_EvaluatecCalSim01_9/index.htmlPDF File:Evaluate Control ResultsSimulationInstructions:Flash File:/content/generator/Course_90012383/Sim_ProE_EvaluateQCSim02_9/Sim_ProE_EvaluateQCSim02_9.swfHTML5 File:/content/generator/Course_90012383/Sim_ProE_EvaluateQCSim02_9/index.htmlPDF File:Select Unload IconHTML  Select the Unload icon at the bottom of the screen. Unload Calibrators and ControlsHTML  From this screen, chose the locations you wish to unload from the Sample Rotor image on the right then select Unload. Or to unload all locations, select Unload All. Remove SamplesHTML Remove the calibrator and control cups from the Sample Rotor. When complete, select the X in the upper-right corner to close the window and continue. Evaluate QC Learn about evaluating QC Instructions:Flash File:/content/generator/Course_90012383/Sim_ProE_LearnEvalQCSim03_9/Sim_ProE_LearnEvalQCSim03_9.swfHTML5 File:/content/generator/Course_90012383/Sim_ProE_LearnEvalQCSim03_9/index.htmlPDF File: