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Syva® Viva-ProE™ System: System Overview Online Training

Locate major hardware components on the system and determine their function so that the operator can successfully set up and operate the instrument. Also provide an overview of software screens, safety considerations, and the Emit® principle. This clinical laboratory training qualifies for continuing education units (CEU).

Welcome to the Syva® Viva-ProE™ System, System Overview online training course. The Viva-ProE System is an automated chemistry analyzer to be used with specific reagents for in vitro diagnostic measurement of analytes in samples of serum, plasma, urine and aqueous standard solutions. This system overview training will review the following features of the Viva-ProE system:  Hardware Software Safety Emit® Principle Select Next to continue. Identify system hardware components List the safety considerations Navigate the system software Upon successful completion of this course, you will be able to: Select Next to continue. Navigate the system software Describe the Emit principle Congratulations. You have completed the Syva® Viva-ProE™ System, System Overview online training course. Listed below are the key points that have been presented. Take time to review the material before you proceed to the final quiz. Identify System Hardware Components: Cuvette Rotor - mixes patient samples with reagents prior to measurement Sample Rotor - holds patient samples Reagent Rotor - holds reagents Cooling Unit / Fill Vessel - used to cool reagents and maintain on-board reagent stability Pipettors - pumps samples and reagents into probes Reagent / Sample Probes - delivers reagents and samples to Cuvette Rotor Panel PC - computer, monitor and touchscreen used to control the Viva-ProE System Barcode Readers - internal barcode reader is included with the system; the external hand-held reader is optional Treated Water Container - holds deionized or distilled water with system solution Diluted Waste / Concentrated Waste Containers - hold the liquid waste generated by the system Lamp Unit - various wavelengths to measure the absorbance in sample tests Safety Cover - must be down when the instrument is in operation Navigate the System Software: Software Login options include: Operator - daily operation functions (most often used login option) Supervisor - all Operator functions plus accept/reject flagged test results and repeat or dilute tests for flagged results Administrator - all Operator and Supervisor functions plus configure system, extend validity and expirations, and authorize deviations Software Overview: Home screen - provides access to all software functions Start of Day screen - displays a list of the start of day activities Reagents screen - displays on-board reagent status; used to load/unload reagents Load Calibration screen - displays the status of calibrations; used to load/unload calibrators and request calibrations Load Controls screen - displays the status of controls; used to load/unload controls and request controls Load Patients screen - displays a list of samples that have been or are pending processing; used to load/unload samples and request tests Results screen - displays test results for calibrations, controls, reagent blanks, and patient results Unload screen - displays a list of loaded samples; used to unload completed samples End of Day screen - displays a list of the end of day activities Other functions from the Home screen include: Login/Logout, Print Screen, access Help, view Events screen, Quality Control, Configure/Customize, view Tasks, Reset and Stop the system, view system Status, and Open/Close the Information Panel List the Safety Considerations: Laser Exposure - barcode readers Biohazards - patient samples, calibrators, controls, diluted and concentrated waste Chemical Hazards - treated water, reagents Electrical Hazards Always wear proper Personal Protective Equipment (PPE) - gloves, eye protection, lab coat Describe the Emit® Principle: Enzyme Multiplied Immunoassay Technique The amount of absorbance from the measured reaction is directly proportional to the amount of drug in the patient sample A higher concentration of drug in the patient sample = higher concentration of unbound enzyme in the solution = higher absorbance measurement A lower concentration of drug in the patient sample = lower concentration of unbound enzyme in the solution = lower absorbance measurement   Select Next to continue.   The Viva-ProE™ System includes the following hardware features: Cuvette, Sample and Reagent Rotors Cooling Unit and Fill Vessel Pipettors Reagent and Sample Probes Panel Personal Computer (PC) and Touchscreen Barcode Readers Treated Water and Diluted Waste Containers (cabinet under Panel PC) Concentrated Waste Container (cabinet on the side of the instrument) Lamp Unit Safety Cover Hardware Components Learn about the hardware components on the Viva-ProE system. Slide NumberText BlocksCalloutsAudio ScriptImage File1 Cuvette Rotor Contains 48 cuvettes that hold 200 µL-400 µL of fluid A heater installed below the rotor keeps the cuvettes at 37°C Mixes the reagents and samples  Rotates the sample for measurement on the instrument Contains a wash arm to wash and dry the cuvette following measurement Waste is deposited in the waste container Select Next to continue. Note: If audio does not automatically start, select the play arrow in the top left to begin.The cuvette rotor contains 48 cuvettes. The system requires a measuring volume inventory between 200 and 400 microliters which is held in the on-board cuvettes. A heater installed below the rotor keeps the cuvettes at 37 degrees Celsius. When in operation, the cuvette rotor initiates the reaction by stirring the reagents and samples delivered by the respective probes and then rotating the sample until it reaches the measurement position on the instrument. After all measurements have been completed, the wash arm washes and dries the cuvette, then deposits the waste in the waste container. Select next to continue.2 Sample Rotor Holds the sample tubes Positions are numbered and displayed on the rotor screens in the software Covered to protect the samples Select Next to continue. The sample rotor holds the sample tubes. All positions are numbered and displayed on the rotor screens in the software. The sample rotor is covered to protect the samples from light and other ambient influences. The sample probe can gain access to the samples through a small opening in the cover. Select next to continue.3 Reagent Rotor Holds 14 mL and 28 mL reagent bottles Positions are numbered on the rotor and reagent locations are assigned in the software Covered to protect the reagents  Contains a cooling element that allows for optimal reagent on-board stability Select Next to continue. The reagent rotor is designed to hold 14 and 28 milliliter reagent bottles. The positions are numbered on the rotor and the reagents are assigned locations on the rotor via the software. The reagent rotor is covered to protect the reagents from light, heat and other ambient influences but the reagent probe can gain access to the reagents through a small opening in the cover. The base of the reagent rotor contains a cooling element that allows for optimal on-board stability. Select next to continue.4 Cooling Unit and Fill Vessel Located behind the front panel and used on the reagent rotor to keep the reagent temperature between 8-12°C Pump circulates cooling liquid from the fill vessel to the cooling unit Monitor fill volume in the fill vessel through a window on the instrument front panel Select Next to continue. Callouts Cooling Unit (behind instrument panel) Cooling fluid fill vessel The Viva-ProE System uses a cooling unit to extend the on-board stability of reagents. The cooling unit keeps the reagent temperature between 8 to 12 degrees celsius and up to an ambient temperature of 25 degrees celsius. The cooling unit is located behind the front panel of the instrument and uses a pump to circulate the cooling liquid. The cooling liquid is held in a fill vessel which can be monitored from a window on the front of the instrument. Select next to continue.5 Pipettor Located in the side cabinet under the Panel PC 1mL syringe for reagents 100 µL syringe for pipetting sample materials Select Next to continue. The pipettors are located inside of the side cabinet under the panel PC. They use a 1 milliliter syringe for pipetting reagents and a 100 microliter syringe for pipetting sample materials. Select next to continue.6 Reagent and Sample Probes Used to aspirate and dispense reagent and sample fluids Delivers fluids to the cuvettes for measurement Rinsed with treated water in between fluid delivery Select Next to continue. Callouts Reagent Probe Sample Probe The Viva ProE system is equipped with a reagent and sample probe. During the aspiration of the reagent or sample, the probes are lowered into the respective rotors and reagents or samples are aspirated. The fluid is then dispensed into the cuvettes for measurement. The probes are rinsed with treated water in between fluid delivery. Select next to continue.7 Panel PC Touch-screen monitor and personal computer (PC) Microsoft® Windows® operating system and the full user-interface Mounted on an adjustable arm Included mini-keyboard and integrated trackball for entering data Select Next to continue. A computer, or Panel PC, is used to run the Viva ProE system. It is a touch-screen monitor complete with a Microsoft Windows operating system and the full user-interface. It is mounted on an adjustable arm and comes with a mini-keyboard and integrated trackball for entering data when needed. Select next to continue.8 Internal Barcode Reader Located at the back of the sample rotor Scans the barcoded sample tubes before fluid is aspirated for measurement Select Next to continue. The Viva ProE system has an integrated internal barcode reader that is located at the back of the sample rotor. It scans the barcoded sample tubes before fluid is aspirated by the sample probe. Select next to continue.9 External Barcode Reader (Optional) Hand-held barcode reader Connected via a USB port on the Panel PC Used to scan barcoded sample tubes prior to placing them on the instrument  WARNING: The hand-held barcode reader emits laser light. Do not look directly at the laser or its reflection from a shiny surface. This causes damage to the eyes. Select Next to continue. An optional external hand-held barcode reader is available for the Viva ProE system. This barcode reader is connected via a USB port on the Panel PC. It is used to scan barcoded sample tubes prior to placing them on the instrument. Select next to continue.10 Treated Water and Diluted Waste Containers Located inside the cabinet underneath the panel PC The Treated Water container holds distilled or deionized water with 12.5 mL of System Solution The Diluted Waste container holds the waste water that was used by the system to wash the cuvettes  Select Next to continue. Callouts Treated Water Container Diluted Waste Container The treated water and diluted waste containers are located inside the cabinet under neath the panel PC. The treated water container holds distilled or deionized water with 12.5 milliliters of System Solution. The diluted waste container holds the waste water that is used by the system to wash the cuvettes. Select next to continue11 Concentrated Waste Container Located in the cabinet on the left side of the Viva-ProE™ System The Concentrated Waste container holds the waste generated from the samples, calibrators and controls  Select Next to continue. The concentrated waste container is located in the cabinet on the left side of the Viva ProE system. The concentrated waste container holds the waste that is generated from the samples, calibrators and controls. Select next to continue12 Lamp Unit Located inside the main instrument cabinet Quartz-iodine lamp Various wavelengths used to measure absorbance in sample tests Changed as part of yearly maintenance activities Select Next to continue. ... The Viva-ProE System uses a quartz-iodine lamp, which uses light of various wavelengths to measure the absorbance in sample tests. The lamp unit is located inside the main instrument cabinet and is changed as part of yearly maintenance. Select Next to continue13Safety Cover Glass safety barrier Must be down whenever the system is operational When complete, select the X in the upper-right corner to close the window and continue. There is a glass safety barrier covering the front of the Viva ProE system. This cover must be down whenever the system is operational. When complete, select the X in the upper right corner to close the window and continue. The software that manages and operates the Viva ProE™System forces authentication by the user prior to operation. Windows Login: Analyzer Software Login: Operator Supervisor Administrator All instrument functions are completed from the Home screen. Software Login Options Learn about the different software login options. Tab TitleTextOperator Operator login is the recommended login for most users. It provides the user with the ability to perform daily operations including: Start of Day tasks Calibration and control measurements Loading reagents and samples Requesting and running tests Evaluating test results Repeat / Re-running tests for flagged results (if applicable)  Supervisor Supervisor login performs Operator functions and: Accepts or rejects flagged test results Repeats or dilutes tests for flagged results   Administrator Administrator login performs Operator and Supervisor functions and: Configures the system Extends validity of reagents, calibrators and controls Authorizes deviations from standard operating procedures When complete, select the X in the upper-right corner to close the window and continue.   Software Overview Learn about the features of the Viva-ProE software. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90010284/sim_VPE_SysOver_SoftwareOverview_swf_800x600_9/sim_VPE_SysOver_SoftwareOverview_swf_800x600_9.swfHTML5 File:/content/generator/Course_90010284/sim_VPE_SysOver_SoftwareOverview_swf_800x600_9/index.htmlPDF File: Contrast enhanced ultrasound has a unique set of terminologies describing the appearance of contrast within the liver. Divided into time-based stages, the arterial phase, portal venous phase, and late phase, show the enhancement occurring within the liver vasculature.   Keep in mind, timing varies due to each patient’s circulatory status and disease process. Cardiac diseases or contrast injection into a small vessel may slow contrast clearing while intracardiac or pulmonary shunting, or hyperdynamic circulation may increase contrast clearing.   Acquire a cine loop to capture arterial, portal venous, and late phase flow. Viva-ProE™ System Safety: Laser exposure, biohazards, chemical hazards, electrical hazards Always wear the appropriate Personal Protective Equipment (PPE) when operating the Viva-ProE System. This includes safety glasses, gloves, and lab coats. Select each tab below to learn about the different hazards present on the Viva-ProE System.   Laser hazards on the Viva-ProE™ System are present with the barcode scanners. Potential hazard to the eyes To avoid damage to the eyes, never look directly at the laser beam or at its reflection from a shiny surface Never point a barcode scanner at someone Potential biohazardous materials on the Viva-ProE System include: Samples Concentrated Waste Diluted Waste Calibrators Controls  Dispose of biohazardous materials according to your facility's protocol.     Chemical waste on the Viva-ProE System includes: Treated Water Reagents  Dispose of chemical waste according to your facility's protocol.     Avoid exposure to shock hazards present on the Viva-ProE™ System. Power off the instrument before proceeding with any electrical system maintenance procedures Select Next to continue.    View these instructions for information on navigating through the self-evaluation tools we call ‘Your Turns’. Click the icon below to start the self-evaluation exercise. Note: This is not part of the final Assessment. Emit® Principle Enzyme Multiplied Immunoassay Technique Measures microamounts of drugs, drug metabolites and naturally-occurring subtstances in human biological fluids Functionality used by the Viva-ProE™ System to analyze and measure samples Emit® Principle Learn about the Emit Principle. The Emit reagent contains antibodies to the specific drug being measured, enzyme-labeled drug that the antibodies can bind to and specific enzyme substrates. The rate of the enzyme activity during the measured reaction causes change in light absorbance. The amount of absorbance is directly proportional to the amount of drug in the patient sample. Antibodies in the reagent specific to the drug being measured are mixed with the patient sample Patient samples with drug immediately bind to the antibody sites Enzyme labeled drug in the reagent (specific to the antibody) is added to the mixture and will bind to any remaining antibody that is not already bound with the patient sample The Emit reagent contains a substrate and coenzyme that reacts with the unbound enzyme. The remaining unbound enzyme from step 3 acts on the substrate which causes a conversion of the coenzyme with an increase in light absorbance during measurement The more unbound enzyme that is in the sample, the higher the final measurement A higher concentration of drug in the patient sample = higher concentration of unbound enzyme in the solution = higher absorbance measurement A lower concentration of drug in the patient sample = lower concentration of unbound enzyme in the solution = lower absorbance measurement When complete, select the X in the upper-right corner to close the window and continue.

  • Pro E
  • Pro-E
  • VivaProE
  • VivaPro E
  • Viva ProE
  • VivaPro-E
  • Viva E
  • VivaE
  • Viva-E
  • DOA
  • DOA Testing
  • Drugs of Abuse Testing
  • cuvette rotor
  • sample rotor
  • reagent rotor
  • cooling unit
  • fill vessel
  • pipettors
  • reagent probe
  • sample probe
  • PC
  • touchscreen
  • touch screen
  • touch-screen
  • barcode reader
  • treated water
  • diluted waste container
  • concentrated waste container
  • lamp unit
  • safety cover
  • windows login
  • software login
  • laser exposure
  • biohazards
  • chemical waste
  • electrical hazards
  • enzyme multipled immunoassay technique