Tissue Preparation System Overview Online Training

The Tissue Preparation Solution consists of the following: Hamilton MICROLAB® STARlet IVD instrument (hardware and software) VERSANT® Tissue Preparation Reagents Select Next to continue. Describe the function of the key technology features of the TPS Distinguish between the icons in the MICROLAB® STARlet software Identify the major system components and describe their function Identify the system consumables and reagents and locate their position on the carriers List the steps of Daily/Weekly and After Run Maintenance Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations.  You have completed the Tissue Preparation System Overview Online Training course.  Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz. Identify the major system components and describe their function Front View of Tissue Preparation System components includes: Power switch Front cover Autoload tray Deck Barcode Reader Interior View of Tissue Preparation System components includes: Carriers Heater/Shaker Units Magnetic separator Waste Assembly Internal Swivel Arm Plate Handler (iSWAP) Pipetting channels The Tissue Preparation System Deck consists of carriers that are removable and in a fixed position. Carriers that are removable include: Plate Carrier Tip Carrier Large Trough Carrier Small Trough Carrier Reagent Carrier Eluates Carrier Sample Carrier The Park Carrier is the only carrier that is in a fixed position. Describe the function of the key technology features of the TPS Key technology features of the Tissue Preparation System include: Autoload - Provides efficient loading of the carriers Air Displacement - Ensures no contact between the liquid and bottom of the plunger due to the presence of air Compression – Induced O-ring expansion (CO-RE)- Provides precise tip and 6-Tube Transport Adapter attachment and release Identify the system consumables and reagents and locate their position on the carriers The Tissue Preparation Solution reagents and consumables: Consumables: Sample Tubes Eluate Collection Tubes Matrix 96-well plate 96-well deep-well plate (DWP) High and Standard Volume Tips Large and Small Reagent Troughs VERSANT® Tissue Preparation Reagent kit is divided into three boxes each with different storage temperatures. Distinguish between the icons in the MICROLAB® STARlet software The Software is used to perform the following tasks: Perform Maintenance Run a Protocol List the steps of Daily/Weekly and After Run Maintenance Daily/Weekly Maintenance tasks include: Access Maintenance Clean Deck Empty Tip Waste Verify Completion After Run Maintenance task include: Remove Reagents Discard consumables Empty Waste Clean Deck, Carriers, and Tip Ejection Plate Select Next to continue. Front View of Tissue Preparation System components includes: Power switch Front cover Autoload tray Deck Barcode Reader Interior View of Tissue Preparation System components includes: Carriers Heater/Shaker Units Magnetic Separator Waste Assembly Internal Swivel Arm Plate Handler (iSWAP) Pipetting Channels Front System Components Learn more about the Front System Components. Base ImageHotspotsText BlocksImage FileFront Cover A hinged transparent cover that provides easy access to the deck. The cover is closed and locked during a run.Power Switch A switch that allows you to power the instrument on or off.Autoload Tray A tray that extends from the front of the deck with track markings to assist the carrier placement.Deck The enclosed work surface of the instrument that is divided into 30 tracks. Samples, reagents, and consumables are loaded onto the deck in carriers at specific track positions.Barcode Reader The onboard barcode reader that automatically scans the carrier and tip tray barcodes during a run. The reader rotates to scan both the horizontal and vertical barcodes. Interior System Components Learn more about the Interior System Components. Base ImageHotspotsText BlocksImage FileHeater/Shaker Units The TPS uses two heater/shaker units.  A Deep-Well Plate (DWP) heater/shaker and a tube heater/shaker.Carriers Each carrier occupies a designated track position on the deck of the instrument.  There are multiple removable carriers and a single fixed position carrier.Magnetic Separator The TPS has a single Magnetic Separator on which the Deep-Well Plate (DWP) is temporarily positioned during a protocol.Waste Assembly The assembly consists of a: Liquid Waste Trough Tip Eject Plate Liquid Waste Container Solid Waste Container iSWAP Transports the 2-mL Deep-Well Plate (DWP) and/or the Matrix 96-well plate to and from required processing locations depending on the protocol selected.Pipetting Channels There are 8 pipetting channels that work in parallel for simultaneous transfer of liquids and tube transport adapters. Félicitations ! Vous avez terminé la formation en ligne de l'étaleur colorateur ADVIA® Autoslide portant sur la présentation du système. Vous trouverez ci-dessous les principaux thèmes qui ont été présentés. Prenez le temps de relire ce document avant de procéder au quiz. The Tissue Preparation System deck consists of carriers that are removable and in a fixed position. Carriers that are removable include: Plate Carrier Tip Carrier Large Trough Carrier Small Trough Carrier Reagent Carrier Eluates Carrier Sample Carrier The Park Carrier is the only carrier in a fixed position. Download and print a copy of the Tissue Preparation System Cue Card. System Carriers Learn about the various carriers on the Tissue Preparation System. Slide NumberText BlocksCalloutsAudio ScriptImage File1The Plate Carrier houses: The Deep-well plate (DWP) The Matrix 96-well plate Note: The Matrix 96-well plate is loaded on the plate carrier depending on the protocol selected.  Select Next to continue . Select each number to review the corresponding text.Note: If audio does not automatically start, select the play arrow in the top left to begin.CalloutsDeep well plateMatrix 96-well plateThe first type of Carrier is referred to as the Plate Carrier. In this Carrier, the Deep-well plate and the Matrix 96-well plate are loaded in the starting position of the run. 2The Tissue Preparation System Deck contains two tip carriers which can accommodate: High volume tips (1000µL) Standard volume tips (300µL)  Select Next to continue.The Tip Carriers accommodate two sizes of tips, high volume and standard volume. 3The Large Trough Carrier contains reagents in the following positions:   Position 1: Lysis Buffer Position 2: Wash 1 Position 3: Wash 2 Position 4: Wash 3 Select Next to continue.    The Large Trough Carrier accommodates 200 ml size troughs which contain reagents. There are four positions that can be used on this carrier. 4The Small Trough Carrier contains reagents in the following positions:   Position 1: (empty) Position 2: FFPE Buffer Position 3: (empty) Position 4: Magnetic Beads Position 5: Elution Buffer  Select Next to continue.     The Small Trough Carrier accommodates 50ml size troughs which contain reagents. There are five positions on this carrier. 5The Reagent Carrier contains reagents in the following positions: Position 31: DNase I Position 32: Proteinase K (PK) Note:  The DNase I mix is loaded depending on the protocol selected.  Select Next to continue.   Select each number to review the corresponding text. CalloutsPosition 31: DNase I Position 32: Proteinase K  The Reagent Carrier accommodates Proteinase K (PK) and DNase I which are temperature sensitive reagents. 6Eluates Carrier: The TPS comes with two eluates carriers each containing 24 tube insert adapters to accommodate 1.5 mL Eppendorf tubes The Eppendorf tubes are used to collect and store eluates Note:  The Eluates Carrier and tubes are loaded depending on the protocol selected.  Select Next to continue. There are two Eluates carriers which can accommodate up to 24 tubes per carrier for a maximum total of 48 tubes.7Sample Carrier and 6-Tube Transport Adapters: The TPS comes with two sample carriers with ring magnets Each carrier is able to accommodate up to 24 tubes in four 6-Tube Transport Adapters  Select Next to continue.  Select each number to review the corresponding text.Callouts6-Tube Transport Adapter Sample Carrier  There are two Sample carriers which can accommodate up to 24 tubes per carrier for a maximum total of 48 tubes. In addition, there are four 6- Tube Transport Adapters in which the sample tubes are directly loaded. 8The Park Carrier is the only carrier that is fixed to the deck.  It contains the following: Position 1: Deep-well plate heater/shaker Position 2: Tube heater/shaker Position 3: Magnetic separator When complete, select the X in the upper-right corner to close the window and continue.Select each number to review the corresponding text.CalloutsDWP heater/shakerTube heater/shakerMagnetic separatorThe last carrier type is referred to as the Park Carrier. This is the only carrier that is in a fixed position on the deck. The stationary items on this carrier include the two heater/shakers and the magnetic separator. L'Autoslide fonctionne en raccord au système ADVIA® 2120i. La préparation de la lame est composée de trois étapes : l'aspiration de l'échantillon, la préparation du frottis puis la coloration du frottis. Lancez la vidéo pour visualiser l'ensemble du processus. Key Technology Features of the Tissue Preparation System include: Autoload - Provides efficient loading of the carriers Air Displacement - Presence of air ensures no contact occurs between the liquid and bottom of the plunger Compression - Induced O-ring Expansion (CO-RE) - Provides precise tip and 6-Tube Transport Adapter attachment and release   Air Displacement Learn more about air displacement. As liquid is aspirated into the tip, it is separated from the plunger by air.   Plunger Air Liquid surface When complete, select the X in the upper-right corner to close the window and continue. L'onglet Fonctions hydrauliques permet d'initier des cycles de rinçage ou d'amorçage de l'Autoslide. The Tissue Preparation Solution reagents and consumables: Consumables: Sample tubes Eluate collection tubes Matrix 96-well plate 96-well DWP High and Standard volume tips Large and Small reagent troughs VERSANT® Tissue Preparation Reagents kit is divided into three boxes each with different storage temperatures. System Consumables Review the system consumables. Tab TitleTextTube Types The TPS accommodates two types of tubes: 1.5-mL collection tube for eluates (Eppendorf) 1.5-mL sample tubes (Sarstedt)    Plate Types The TPS accommodates two types of plates: Matrix 96-well plate (split method) 96-well deep-well plate (DWP)    Tip Volumes The TPS accommodates two sizes of tip volumes: High volume tips (1000 µL) Standard volume tips (300 µL) CAUTION: Do not use tips other than those specified and supplied by Siemens.  Using improper tips can cause the run to fail and damage to the system.  The tips are for single use and cannot be reused.  Reagent Trough Sizes The TPS accommodates two sizes of Reagent Troughs: Large Reagent Troughs (200mL) Small Reagent Troughs (50mL) When complete, select the X in the upper-right corner to close the window and continue.   System Reagents Box 1 Learn more about the System Reagents. Base ImageHotspotsText BlocksImage FileThe VERSANT® Tissue Preparation Reagents Kit, Box 2 contains the following component: Proteinase K (1 Tube) Storage Temperature: 2°   to 8° C The VERSANT® Tissue Preparation Reagents Kit, Box 1 contains the following components: Lysis Buffer, Elution Buffer, and FFPE buffer Wash Buffer 1, 2, and 3 Magnetic Beads Storage Temperature: 15° to 30°C  The VERSANT® Tissue Preparation Reagents Kit, Box 3 contains the following components: DNase I DNase I Buffer 4 Tubes to make DNase I mix Storage Temperature: -30° to -10°C    Select each tab below to learn more about the functionality of each main icon in the Hamilton MICROLAB® STARlet software. The Microlab STAR Maintenance Icon is selected to access the screens used in Daily/Weekly Maintenance.   The Microlab STAR RUN Icon is selected to perform any of the extraction protocols. When complete select Next to continue.     The Tissue Preparation System required maintenance procedures are: Daily Maintenance Weekly Maintenance After Run Maintenance Warning: Do not allow any waste, surface, or material that contains or may potentially contain guanidine thiocyanate (such as Lysis Buffer and Wash 1) to have contact with bleach, Dispatch, or any agent containing sodium hypochlorite. Cyanide gas may result from this mixture. Do not dispose of unprocessed reagents containing guanidine thiocyanate in the sink. Download and print a copy of the Tissue Preparation System Maintenance Checklist. Daily/Weekly Maintenance Tasks Learn about Daily/Weekly Maintenance tasks on the TPS. Checklist TitleChecklist TypeChecklist ContentAccess MaintenanceHTML   Select each checkbox to learn more about Daily/Weekly Maintenance tasks. Select the Microlab STAR Maintenance Icon > Select the required maintenance displayed on the Hamilton Maintenance and Verification run screen > and then select the Green arrow at the top of the maintenance window to initiate maintenance. The software will prompt the user through the remaining maintenance steps. Note: If both daily and weekly maintenance are required, select Weekly Maintenance. Clean DeckHTML Use Microcide SQ to clean the deck and tip eject plate. Rinse using deionized water and follow by spraying 70% ethanol. CAUTION: Do not directly spray the TPS instrument with Microcide SQ. Microcide SQ can damage electrical boards and connectors.  Use a clean lint-free laboratory wipe.  Empty Tip WasteHTML Remove and clean the solid waste container. Line the solid waste container with a new biohazard bag and place the solid waste container back on the system.  Dispose of biohazard bag according to your laboratory and local regulatory protocols. Note: Ensure that the biohazard bag is laying flat against the container.  Verify CompletionHTML When the Daily or Weekly Maintenance - successful window displays, select OK, which verifies that the process has completed successfully. When complete, select the X in the upper-right corner to close the window and continue.   Maintenance After a Run Tasks Learn about Maintenance After Run Tasks on the TPS. Checklist TitleChecklist TypeChecklist ContentRemove ReagentsHTML Select each checkbox to learn more about Maintenance After a Run tasks. Remove the Proteinase K from the Control Carrier. Cap and store between 2°C and 8°C. Note:  If running an RNA or DNA/RNA split protocol, discard the DNase I mixture in the biohazard bag located in the solid waste container.   Discard ConsumablesHTML Discard the DWP and 1.5-mL Sarstedt screw cap tubes according to your laboratory protocol for biohazard waste disposal.  Empty WasteHTML   Remove and clean the solid waste container. Line the solid waste container with a new biohazard bag and place the solid waste container back on the system.  Dispose of biohazard bag according to your laboratory and local regulatory protocols. Note: Ensure that the biohazard bag is laying flat against the container.  Clean DeckHTML Use Microcide SQ to clean the deck, carriers, and tip eject plate. Rinse using deionized water and follow by spraying 70% ethanol. CAUTION: Do not directly spray the TPS instrument with Microcide SQ. Microcide SQ can damage electrical boards and connectors.  Use a clean lint-free laboratory wipe. When complete, select the X in the upper-right corner to close the window and continue.