VERSANT® kPCR Molecular System: Sample Preparation Module System Overview Online Training

From the Second Menu, use STORED INFORMATION options to access results and statistics stored in the RAPIDPoint 300 Systems. For example you can view stored data for patient and QC results and QC reference limits. You can also access data for the last calibration as well as reagent module statistics. Let’s take a closer look at stored results and statistics. The VERSANT® kPCR Molecular System consists of the following: Sample Preparation (SP) Module and computer The SP module performs the nucleic acid isolation and purification steps. Amplification and Detection (AD) Module and computer The AD module performs amplification of  the nucleic acids using kPCR technology VERSANT Sample Preparation Reagents Assay Specific Reagents Select Next to continue.   Describe the function of the key technology features of kPCR Describe the kPCR extraction process Distinguish between the different carriers and their use Identify System consumables and reagents Identify the main tabs of the SP Module software Identify the major components of the SP module and describe their function List the steps of Daily/Weekly and After Run Maintenance Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations.  You have completed the VERSANT® kPCR Molecular System SP Module System Overview Online Training course .  Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz..   Identify the major components of the SP module and describe their function Front View of SP Module components includes: Power Switch for the SP Module Power Switch for the heater/shaker Front cover Auto load tray Deck Barcode Reader Interior View of SP Module components includes: Carriers Heater/shaker Unit Magnetic Separator Waste Assembly Internal Swivel Arm Plate Handler (iSWAP) Pipetting Channels Distinguish between the different carriers and their use   Carriers that are removable include: Plate Carrier Tip Carrier Reagent Trough Carrier Control Carrier Sample Carrier The Incubation and Separation Assembly is the only carrier in a fixed position. Describe the function of the key technology features of kPCR Key Technology Features of the VERSANT® kPCR Molecular System SP Module include: Auto load - Provides efficient loading of the carriers Air Displacement - Presence of air ensures no contact occurs between the liquid and bottom of the plunger Compression - Induced O-ring Expansion (CO-RE) - Provides precise tip and 6-Tube Transport Adapter attachment and release Describe the kPCR extraction process The VERSANT Sample Preparation Reagents along with the Assay Specific Reagents extract RNA or DNA from various types of patient samples depending on the Protocol being selected. Identify System consumables and reagents The VERSANT kPCR Molecular System reagents and consumables: VERSANT kPCR Molecular System consumables: 96-well DWP Plates and  96-well PCR Plates 1000µL and 300µL volume tips Large and Small reagent troughs VERSANT Sample Preparation Reagents kits: VERSANT Sample Preparation 1.0  Reagents kit VERSANT Sample Preparation 1.2 Reagents kit Identify the main tabs of the SP Module software The VERSANT® kPCR Molecular System SP software consists of the following: Protocol Tab System Tab Reagent Inventory Tracking List the steps of Daily/Weekly and After Run Maintenance Daily/Weekly Maintenance tasks include: Access Maintenance Clean Desk Empty Tip Waste Verify Completion After Run Maintenance tasks include: Remove Samples Remove Reagents Discard Consumables Empty Waste Clean SP Module Select Next to continue.   Front View of SP Module components includes: Power Switch for the SP Module Power Switch for the heater/shaker Front cover Auto load tray Deck Barcode Reader   Interior View of SP Module components includes: Carriers Heater/Shaker Unit Magnetic Separator Waste Assembly Internal Swivel Arm Plate Handler (iSWAP) Pipetting Channels Front System Components Learn about the Front System Components. Base ImageHotspotsText BlocksImage FilePower Switch for the heater/shaker  Allows you to power the heater/shaker on or off. Power Switch for the SP Module Allows you to power the instrument on or off.  Allows you to power the instrument on or off.  Allows you to power the instrument on or off.    Front Cover Consists of a hinged transparent cover providing easy access to the deck.  The cover is closed and locked during a run. Autoload Tray A tray that extends from the front of the deck with track markings to assist the carrier placement.  The Autoload tray is divided into 30 tracks along with etched deck symbols. Samples, reagents, and consumables are loaded onto the Autoload tray in carriers at specific track positions.  Deck The enclosed work surface of the instrument is divided into 30 tracks.  Samples, reagents, and consumables are loaded onto the deck in carriers at specific track positions by the autoloader.    Barcode Reader The onboard barcode reader automatically scans the carriers, reagents, and consumable barcodes during a run for positive identification. The reader rotates to scan both horizontal and vertical barcodes. Interior System Components Learn more about the Interior System Components. Base ImageHotspotsText BlocksImage File Carriers Each carrier occupies a designated track position on the deck of the instrument.  There are removable carriers and a fixed position carrier.  Heater/Shaker Unit The SP Module uses one heater shaker unit. Magnetic Separator The SP Module has a single Magnetic separator that the DWP is temporally positioned on during a protocol. Waste Assembly The assembly consists of the following: Liquid waste trough and tip eject plate Liquid waste container Solid waste container iSWAP Transports the 2-ml DWP(s) and/or the 96-well PCR plate (s) to and from required processing locations depending on the protocol selected.Pipetting Channels There are eight Pipetting channels that work in parallel for simultaneous transfer of liquids. The deck of the SP module consists of carriers that are removable and a single carrier in a fixed position. Carriers that are removable include: Plate Carrier Tip Carrier Reagent Trough Carrier Control Carrier Sample Carrier The Incubation and Separation Assembly is the only carrier in a fixed position. System Carriers Learn about the various carriers on the SP module. Slide NumberText BlocksCalloutsAudio ScriptImage File1The Plate Carrier houses: Two Deep-well plates (DWP) Up to two 96-well PCR plates 300µL tip tray Note: The carrier has the capacity to hold two deep-well plates and up to two 96-well PCR plates depending on the protocol selected. Select  Next to continue . Select each number to review the corresponding text.Note: If audio does not automatically start, select the play arrow in the top left to begin.CalloutsDeep-well plate (DWP)PCR plate300µL tip trayThe first type of carrier is referred to as the Plate Carrier. In this Carrier, two deep well plates and up to two 96-well PCR plates are loaded in the starting position of the run. There is also one 300µL tip tray.2The SP Module deck contains two Tip Carriers which can accommodate:   1000µL tips 300µL tips Select Next to continue.   The Tip Carriers accommodate two sizes of tips: 1000µL and 300µL tips.3The Large Trough Carrier contains reagents in the following positions: Position 1: Lysis Buffer Position 2: Wash 1 Position 3: Wash 2 Position 4: Wash 3 Select Next to continue.   The Large Trough Carrier accommodates 200 ml size troughs which contain reagents. There are four positions that can be used on this carrier. 4The Small Trough Carrier contains reagents in the following positions: Position 1: (empty) Position 2: (empty) Position 3: (empty) Position 4: Magnetic Beads Position 5: Elution Buffer Select Next to continue.  The Small Trough Carrier accommodates 50ml size troughs which contain reagents. There are five positions on this carrier but only two positions are currently used for reagents. 5The Control Carrier contains reagents used by the SP Module. The Control Carrier holds assay-specific reagents in the appropriate positions and Proteinase K in position 32. Note: The Control carrier is the same as a 32-Position Sample Carrier, but is used with special adapters because the controls are in 2 ml tubes. Select Next to continue. Select the number to review the corresponding text.CalloutsProteinase KThe Control carrier transports reagent tubes used by the SP Module. A 32-Position Sample Carrier should be designated as the control carrier on the SP module.6The SP Module Deck contains two Sample Carriers which can accommodate: 24   samples 32 samples   Select Next to continue.  The SP Module can accommodate up to 4 sample carriers. The SP Module supports two types of sample carriers: 24-sample and 32-sample carriers. 7The Incubation and Separation Assembly is the only carrier that is fixed to the deck.  It contains the following: Heater/shaker Unit Magnetic separator Two Park Positions When complete, select the X in the upper-right corner to close the window and continue.Select each number to review the corresponding text.CalloutsHeater/shaker UnitMagnetic SeparatorTwo Park PositionsThe last carrier type is referred to as the Incubation and Separation Assembly. This is the only carrier that is in a fixed position on the deck. The stationary items on this carrier include the heater/shaker and the magnetic separator. Key Technology Features of the VERSANT® kPCR Molecular System SP Module include: Autoload - Provides efficient loading of the carriers Air Displacement - Presence of air ensures no contact occurs between the liquid and bottom of the plunger Compression - Induced O-ring Expansion (CO-RE) - Provides precise tip attachment and release Air Displacement Learn more about Air Displacement.  As liquid is aspirated into the tip, it is separated from the plunger by air.   Plunger Air Liquid surface When complete, select the X in the upper-right corner to close the window and continue.   The VERSANT kPCR Molecular System reagents and consumables include: VERSANT kPCR Molecular System consumables: 96-well DWP Plates and  96-well PCR Plates 1000µL and 300µL volume tips Large and Small reagent troughs VERSANT Sample Preparation Reagents kits: VERSANT Sample Preparation 1.0  Reagents kit VERSANT Sample Preparation 1.2 Reagents kit System Consumables Learn about the System Consumables. Tab TitleText96-well Plates The SP Module accommodates two sizes of 96 well plates: 96-well PCR plate 96-well DWP    Tip volumes The SP Module accommodates two sizes of tips: 1000µL 300µL    Regent Trough Sizes The SP Module  accommodates two sizes of reagent troughs: Large Reagent Troughs (200mL) Small Reagent Troughs (50mL) When complete, select the X in the upper-right corner to close the window and continue.      Sample Preparation 1.0 Reagents kit Learn about Sample Preparation 1.0 Reagents kit. Base ImageHotspotsText BlocksImage FileVERSANT® Sample Preparation 1.0 Reagents Box 1 contains the following components:   Lysis Buffer, Elution Buffer Wash Buffer 1, 2, and 3 Magnetic Beads   Storage Temperature: 15° to 30°C The VERSANT Sample Preparation Reagents Kit, Box 2 contains the following component:   Proteinase K 1 Tube Barcode card   Storage Temperature: 2–8°C    Sample Preparation 1.2 Reagents kit Learn about Sample Preparation 1.2 Reagents kit. Base ImageHotspotsText BlocksImage FileVERSANT Sample Preparation 1.2 Reagents Box 1 contains the following components: Lysis Buffer, Elution Buffer Wash Buffer 1, 2, and 3 Pretreatment Buffer Magnetic Beads Storage Temperature: 15° to 30°C   The VERSANT Sample Preparation 1.2 Reagents Kit, Box 2 contains the following component: Proteinase K 1 Tube Barcode card Storage Temperature: 2–8°C    The Versant kPCR Molecular System SP software consists of the following: Protocol Tab Select the protocol Enter and edit kit lot information System Tab Perform required maintenance tasks Access the Event Log Reagent Inventory Tracking Track reagent barcodes Monitor reagent volumes Calculate tests remaining for sample preparation and assay reagents SP Module Software Simulation Learn how to access the SP Software. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90005034/sim_kPCR_SPSWOverviewdemo3_800x600/sim_kPCR_SPSWOverviewdemo3_800x600.htmHTML5 File:/content/generator/Course_90005034/sim_kPCR_SPSWOverview_HTML5_800x600/index.htmlPDF File: The SP module required maintenance procedures are: Daily Maintenance Weekly Maintenance Maintenance After a run Caution: Do not allow any waste, surface, or material that contains or may potentially contain Guanidine Thiocyanate (such as Lysis Buffer and Wash 1) to have contact with bleach, Dispatch, or any agent containing sodium hypochlorite. Cyanide gas may result from this mixture. Do not dispose of anything containing Guanidine Thiocyanate in the sink.  Waste containing Guanidine Thiocyanate should only be disposed of in a chemical waste container.   Download and print a copy of the SP Maintenance Checklist. Daily/ Weekly Maintenance Learn more about Daily/Weekly Maintenance tasks on the SP Module. Checklist TitleChecklist TypeChecklist ContentAccess MaintenanceHTML Select each checkbox to learn more about Daily/Weekly Maintenance tasks on the SP Module. Select the System Tab > Select the check box for daily maintenance > and then select the Green arrow at the top of the maintenance window to initiate maintenance. The software will prompt the user through the remaining maintenance steps. Note: If both daily and weekly maintenance are required, select Weekly Maintenance.  Clean DeckHTML Use Microcide SQ to clean the deck and tip eject plate. Rinse using deionized water and follow by spraying with 70% ethanol. CAUTION: Do not directly spray the SP Module deck with Microcide SQ. Microcide SQ can damage electrical boards and connectors.  Use a clean lint-free laboratory wipe.  Empty Solid WasteHTML Remove and clean the solid waste container. Line the solid waste container with a new biohazard bag and place the solid waste container back on the system.  Dispose of the biohazard bag according to your laboratory and local regulatory protocols. Note: Ensure that the biohazard bag is laying flat against the container.   Verify CompletionHTML When the Daily or Weekly Maintenance - successful dialog box displays, select OK, which verifies that the process has completed successfully. When complete, select the X in the upper-right corner to close the window and continue.   Maintenance After A Run Learn about Maintenance After a Run tasks on the SP Module. Checklist TitleChecklist TypeChecklist ContentRemove SamplesHTML Select each checkbox to learn more about Maintenance After a Run tasks on the SP Module. Remove Samples from Sample Carrier. Note: Cap any samples you wish to retain and return them to the freezer.  Discard unwanted sample tubes into biohazard waste according to the practices of your institution and in compliance with all local and regulatory requirements.  Remove ReagentsHTML Remove the used VERSANT Sample Preparation Reagents from the deck of the SP Module Reagent and Control Carrier. Depending on the Protocol selected, there may be additional assay specific reagents that may also need to be removed. Note: Any remaining reagents that you wish to keep should be capped and returned to the recommended temperature conditions.  Discard all used reagents and troughs (liquid and solid waste)  according to the practices of your institution and in compliance with all local and regulatory requirements. Discard ConsumablesHTML Discard the DWP into the biohazard waste container.  Empty Solid WasteHTML Remove and clean the solid waste container. Line the solid waste container with a new biohazard bag and place the solid waste container back on the system.  Dispose of biohazard bag according to your laboratory and local regulatory protocols. Note: Ensure that the biohazard bag is laying flat against the container.   Clean SP ModuleHTML Use Microcide SQ to clean the deck, carriers, and tip eject plate. Rinse using deionized water and follow by spraying with 70% ethanol. CAUTION: Do not directly spray the SP Module  with Microcide SQ. Microcide SQ can damage electrical boards and connectors.  Use a clean lint-free laboratory wipe. When complete, select the X in the upper-right corner to close the window and continue.   Beads and Reagents Onboard 화면은 장비 온보드 상에 있는 시약과 비드에 대한 자세한 정보를 제공합니다. 여러분은 테스트 이름, 키트 로트, 비드 또는 시약 로트, 남아 있는 테스트 수, 키트 만료 일자, 소비 기한, 마지막 어저스트먼트 날짜, 어저스트먼트 말료 일자, 그리고 캐로우셀 위치를 확인할 수 있습니다. 이 로그는 기록 또는 문제 해결을 위해서 인쇄할 수 있습니다. Beads and Reagents Onboard 화면에 대해 자세히 배우려면 아래 링크를 선택하세요. The VERSANT Sample Preparation Reagents extract RNA or DNA from various types of patient samples depending on the Protocol being selected.  This enables the automated extraction of either RNA or DNA.   kPCR Extraction Process Review the extraction process of nucleic acids from patient samples.   Select the play arrow to begin the animation.  When complete, select the X in the upper-right corner to close the window and continue.