DCA Vantage® Analyzer Microalbumin/Creatinine Calibration and Quality Control Online Training

This is a patient with a 2 week history of back pain after a fall and with history of breast carcinoma. The bone scan results were abnormal activity L2 vertebral body, mets versus post traumatic fracture.  Minimal focal activity was found at the left 7th rib which is suspicious for mets versus trauma. The patient was given 30 milli curies of Tc99m MDP, with a 3 hour delay. A planar whole body was acquired with spot views of the spine and pelvis. The results of an MRI Lumbar spine suspicious for possible bone marrow metastases. FDG PET scan was ordered for restaging prior to treatment planning using 10 milli curies of FDG. The scan results were no abnormalities seen. Since the FDG PET can be negative in sclerotic or osteoblastic lesions, further evaluation with F18 sodium fluoride was recommended. The sodium fluoride PET bone scan was acquired with 10 millicuries given. The scan results showed disseminated metastatic bone disease. The scan was acquired at a 110 minutes delay. Patient received appropriate systemic therapy after accurate identification of the presence and extent of bone disease with F18 sodium fluoride PET bone imaging.   Welcome to the DCA Vantage® Analyzer Microalbumin/Creatinine Calibration and Quality Control Online Training course. The DCA Vantage® Analyzer is designed to automatically perform a number of internal optical, electronic and mechanical checks, including calibration verification, with every test.  In addition, with every new reagent lot, operators can scan the calibration card and the analyzer performs an automatic lot-specific calibration.  Quality Control testing assures the integrity of the reagents and detects errors that may result from improper testing technique.    Note: Wear personal protective equipment, including safety glasses, and gloves. Use universal precautions when working with biohazardous materials. Note: This course includes informaiton on Options and Functions in the system which may require security approval from your facility in order to access. Select Next to continue.   Identify the conditions under which Microalbumin/Creatinine Quality Controls should be run Identify the steps to perform Calibration for Microalbumin/Creatinine Identify the steps to perform Quality Control for Microalbumin/Creatinine Recall Microalbumin/Creatinine Calibration Data Recall Microalbumin/Creatinine Quality Control Data Upon successful completion of this course, you will be able to: Select Next to continue. Al termine di questo corso, sarete in grado di: Riconoscere il significa della sezione chimica clinica. Identificare i test di normale esecuzione in Chimica Clinica Identificare i componenti delle reazioni chimiche. Riconoscere i più comuni metodi di rilevamento di reazione. Identificare i principi di immunochimica e di potenziometria. Iniziamo esaminando il significato di chimica clinica. Congratulations.  You have completed the DCA Vantage® Analyzer Microalbumin/Creatinine Calibration and Quality Control Online Training Course. Note: These options must be enabled in the system. Security approval must be enabled in order to access these functions. Perform Microalbumin/Creatinine Calibration   The DCA Vantage® Analyzer automatically performs a number of internal optical, electronic and mechanical checks including calibration verification with every test. With every new reagent lot, the analyzer performs an automatic lot-specific calibration when the calibration card is scanned. Values for reagent calibration parameters are encoded on the calibration card provided with each box of the reagent cartridges. Scan the calibration barcode before using the reagent cartridges. Scanning the calibration card automtically records the information into the analyzer. The analyzer stores calibration information for up to 16 distinct reagent lots. If you do not scan the calibration curve into the system for the particular lot number of cartridges that are in use, the system software prompts you to scan the calibration card. You must scan the calibration card for a lot of reagent cartridges before you can use the lot on analyzer.    Note: Wear personal protective equipment, including safety glasses, and gloves. Use universal precautions when working with biohazardous materials.   To scan the Microalbumin/Creatinine calibration card: Locate the dot on the system next to the barcode track. Locate the barcode on the calibration card. Hold the card so that the barcode faces to the right. Insert the Calibration card into the top of the barcode track. Hold the Calibration card gently against the right side of the track and smoothly slide the card down. A beep sounds to signal a successful scan. To return to the Home screen, select OK Recall Calibration Data Use the Calibration Data screen to access calibration data including scan date and time, and lot number for DCA Vantage® Microalbumin/Creatinine test. To view calibration data: Select Recall from the Home screen Select Calibration Data from the Recall menu Select Microalbumin/Creatinine to display calibration data Highlight the calibration data that you want to display then select View. Select Print to print the calibration data Select Recall to return to the Recall menu Run Microalbumin/Creatinine Quality Controls All control results must be within the acceptable range before any patient sample is tested and the results are reported. If a control result is out of range, troubleshoot the system, correct any problems identified, and run the controls again. When control results are within range, you can test and report patient samples. The DCA Vantage® system can display a reminder message when a QC test is due. You can set the QC test reminder message to display a QC reminder only or require a QC test for selected control tests. If you set the system to require a QC, you must run and pass the QC test before you can continue with patient tests. NOTE: Prepare the controls according to the manufacturer’s instructions. Good laboratory practice dictates that a quality control program be established in all laboratories. The program should consist of assaying control material, evaluation of control results and acceptable limits on controls. Reagent Cartridge storage and sample collection and handling practices are also incorporated into the quality control program.     To prepare Quality Control solutions: Prepare the Controls according to the manufacturer’s instructions Remove and unwrap the capillary holder from the Reagent Kit. Mix the sample well by inversion or using a tube mixer. Open the control bottle. Avoid introducing air bubbles into the sample. While applying pressure to the bulb of the dropper (found in the control kit), insert the tip of the dropper into the control solution. Release the pressure on the bulb to aspirate a small amount of control solution. Fill the glass capillary tube by touching it to the tip of the dropper and fill the tube. Touch only the tip of the capillary tube to the control material. If an air bubble is present in the filled tube, discard the capillary holder and fill a new one. Prevent the control material from coming in contact with the plastic part of the capillary holder. If control material comes in contact with the capillary holder, discard the capillary holder. Squeeze any excess control material out of the dropper into the control bottle. Close the control bottle. Wipe any control solution off the sides of the glass capillary tube using a lint-free tissue. Do not touch the tissue to the open end of the tube. Contact with the open end could result in a loss of the sample. Note: After opening the foil pouch, the reagent cartridge must be used within 10 minutes. Inspect the capillary holder for the presence of any bubbles. If bubbles are obvious, discard the capillary and repeat the collecting procedure. Place the capillary holder in the correct position to insert it into the reagent cartridge. Insert the capillary holder into the reagent cartridge until the holder gently snaps into place. Avoid harsh insertion of capillary holder. Do not dislodge the sample in the glass capillary because erroneous results may occur. Follow these steps to run a control test: Ensure you are at the Home screen; otherwise the system does not recognize that a QC test is being run. The system will not save data to memory or prompt that a QC test due (if reminder is set). Locate the control card. One side of the control card is for a normal control and the other side is for an abnormal control. Lot specific Mean and Range Values for Microalbumin/Creatinine are found on the control card in the control kit. Locate the dot on the system next to the barcode track. Locate the barcode on the Control card. Hold the card so that the barcode faces to the right. Insert the Control card into the top of the barcode track. Hold the Control card gently against the right side of the track and smoothly slide the card down. Scan the Control Card. A beep sounds to signal a successful scan. If no beep sounds, repeat the scanning procedure. Scan the reagent cartridge and insert into the reagent cartridge compartment. Quality Controls Conditions Run quality control under these conditions: At regular intervals determined by the laboratory procedures When using a new shipment of reagents When using a new lot number of reagent Each time a calibration card is scanned Whenever test results are in doubt When training new operators Recall Quality Control Data  Use the Control Tests Data screen to access Quality Control data including test results, run date and time, lot number, and expiration date. Use the Control Tests Data screen to access Quality Control data including test results, run date and time, lot number, and expiration date. The steps required to access the Control Tests Data screen is the same sequence as to recall Calibration Data. Select Next to continue.   Gratulation! Sie haben den Abschnitt RapidPoint Software Übersicht im Online Training Kursus absolviert. Nehmen Sie sich Zeit, das Material zu überprüfen, bevor Sie mit dem abschließenden Test fortfahren. The analyzer stores calibration information for up to 16 distinct reagent lots of DCA Microalbumin/Creatinine reagent cartridges. Use the lot-specific calibration card provided in the kit to scan the calibration barcode information prior to scanning the first reagent cartridge barcode. Scanning the calibration card allows access to the appropriate calibration parameter values and calibration curve for the particular lot of reagent in use. The values for reagent calibration parameters are encoded on the calibration card provided with each kit box of reagent cartridges. Scanning the calibration card automatically records the information into the DCA® Vantage analyzer. The calibration card for a lot of reagent cartridges must be scanned before that lot can be used on the system. The calibration card contains the reagent lot number, mean values, value ranges, and expiration date in both barcode and printed format. Note: If you do not scan the calibration curve into the system when using a new lot of Microalbumin/Creatinine reagents, the system software prompts you to scan the calibration card. It is necessary to scan only one calibration barcode per lot number.  Additional cartridges or boxes from the same lot number may be used without rescanning the calibration card information.   Scanning the Hemoglobin A1c Calibration Card Learn about scanning the Microalbumin Calibration Card. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90006388/1_sim_DCAV_ScanMicCrCalCard_HTML5_800x600_9/1_sim_DCAV_ScanMicCrCalCard_HTML5_800x600_9.swfHTML5 File:/content/generator/Course_90006388/1_sim_DCAV_ScanMicCrCalCard_HTML5_800x600_9/index.htmlPDF File: La chemiluminescenza è un metodo di rilevazione speciale impiegata per reazioni chimiche che producono fotoni di luce quando avviene la reazione. Il rilevatore misura la quantità di luce prodotta per determinare la quantità di analita presente. In questo caso, non c'è luce sorgente incidente in quanto la reazione chimica produce da sè la luce che viene misurata. Selezionare Avanti per continuare. Use the Calibration Data screen to access calibration data including scan date and time, and lot number for Microalbumin/Creatinine calibration. Recalling Calibration Data Learn how to view calibration data for Microalbumin/Creatinine. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90006388/2_sim_DCAV_RecallMicCrCalData_HTML5_800x600_9/2_sim_DCAV_RecallMicCrCalData_HTML5_800x600_9.swfHTML5 File:/content/generator/Course_90006388/2_sim_DCAV_RecallMicCrCalData_HTML5_800x600_9/index.htmlPDF File: Al completar este curso usted podrá identificar los componentes del sistema de automatización Aptio ™ y su función, distinguir las etiquetas de seguridad en el sistema, y ​​describir las principales áreas de la interfaz de usuario. Seleccione Siguiente para continuar. Good laboratory practice dictates that a quality control program be established in all laboratories. The program should consist of assaying control material, evaluation of control results and acceptable limits on controls. Reagent Cartridge storage and sample collection and handling practices also are incorporated into the quality control program. Refer to your laboratory quality assurance program to ensure quality throughout the entire testing process. Follow the manufacturer's storage and handling instructions for quality control material. Improper storage and handling of control materials can cause erroneous results. Refer to the control material package insert for proper handling instructions. Run Quality Controls under the following conditions: Regular intervals determined by the laboratory procedures Using a new shipment of reagents Using a new lot number of reagent Scanning a calibration card Test results are in doubt Training new operators Caution: When working with biohazardous materials, always use universal precautions. Wear personal protective equipment, including safety glasses, and gloves. Note: After opening the foil pouch, the reagent cartridge must be used within 10 minutes. Running Quality Control Learn how to run Quality Control. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90006388/3_sim_DCAV_RunMicCrQC_HTML5_800x600_9/3_sim_DCAV_RunMicCrQC_HTML5_800x600_9.swfHTML5 File:/content/generator/Course_90006388/3_sim_DCAV_RunMicCrQC_HTML5_800x600_9/index.htmlPDF File: Preparing Quality Control Solutions Learn how to prepare Quality Control solutions. Prepare the Controls according to the manufacturer’s instructions. Remove and unwrap the capillary holder from the Reagent Kit. Mix the control sample well by inversion or using a tube mixer. Open the control bottle. Avoid introducing air bubbles into the sample. While applying pressure to the bulb of the dropper (found in the control kit), insert the tip of the dropper into the control solution. Release the pressure on the bulb to aspirate a small amount of control solution. Fill the glass capillary tube by touching it to the tip of the dropper and fill the tube. Touch only the tip of the capillary tube to the control material. If an air bubble is present in the filled tube, discard the capillary holder and fill a new one.  Prevent the control material from coming in contact with the plastic part of the capillary holder.  If control material comes in contact with the capillary holder, discard the capillary holder. Squeeze any excess control material out of the dropper into the control bottle. Close the control bottle. Wipe any control solution off the sides of the glass capillary tube using a lint-free tissue. Do not touch the tissue to the open end of the tube. Contact with the open end could result in a loss of the sample. Inspect the capillary holder for the presence of any bubbles. If bubbles are obvious, discard the capillary and repeat the collecting procedure. Place the capillary holder in the correct position to insert it into the reagent cartridge. Insert the capillary holder into the reagent cartridge until the holder gently snaps into place. Avoid harsh insertion of capillary holder. Do not dislodge the sample in the glass capillary because erroneous results may occur.   When complete, select the X in the upper-right corner to close this window and continue.  La cadena Aptio proporciona la entrega automatizada de muestras para todos los módulos y los instrumentos analíticos. Se compone de diferentes elementos para la vía, que controlan el movimiento del tubo dentro de la cadena. Los transportadores o pucks se utilizan para transportar los tubos de muestra en la cadena. Los transportadores o pucks tienen un identificador único que identifica electrónicamente a través de un ID de frecuencia de radio, gracias a un chip RFID incorporado en cada soporte. El ID de soporte de muestras (RFID) y la muestra Identificación tubo (código de barras) están emparejados con el fin de realizer el seguimiento de la muestra, mientras está en la cadena. Seleccione los enlaces de abajo para aprender acerca de los componentes de la cadena y el soporte de la muestra. Use the Control Tests Data screen to access Quality Control data including test results, run date and time, lot number, and expiration date. The steps required to access the Control Tests Data screen is the same sequence as to recall Calibration Data. Select Next to continue.   Ahora que ha revisado los principales componentes de hardware del sistema Aptio de automatización, vamos a ver la interfaz de usuario y la pantalla de resumen. La pantalla Resumen es la pantalla principal del sistema. Se muestra el estado de funcionamiento general del sistema y del módulo y de los analizadores individuales. También se muestra el estado de cada uno de los veinte carriles del módulo de entrada / salida. Además, los botones de navegación están disponibles para acceder a los menús de software. Seleccione el enlace de abajo para aprender sobre las áreas clave de las pantallas del software. The DCA Vantage® Analyzer displays a reminder message when a QC test is due. The QC test reminder message can be set to display a QC reminder only or require a QC test for selected control tests. If the system is set to require QC, you must run and pass the QC test before proceeding with patient tests. Control results must be within the acceptable range before any patient sample is tested and the results are reported. If a control result is out of range, troubleshoot the system, correct the problems then run the controls again. After the control results are within range, you can test and report patient samples. Note: These options must be enabled in the system. Security approval is required in order to access these functions. Note: Prepare the controls according to the manufacturer’s instructions. After opening the foil pouch, the reagent cartridge must be used within 10 minutes. Select Next to continue. .