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Sysmex® CA-600 Series Analyzer Calibration Online Training

Perform the calibration procedure, review and evaluate calibration data for accurate patient results.

Welcome to the Sysmex® CA-600 Series System Calibration Online Training course. Some of the test methods run on the analyzer need to be calibrated prior to using a new reagent lot number. To perform a calibration, the system will: Analyze serial dilutions of a reference standard Plot the raw analysis data Calculate final results in concentration or percent activity Select Next to continue. Describe the process to perform a calibration Identify key areas of the Standard Curve screen List the steps to manually enter data in a standard curve Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations.  You have completed the Sysmex® CA-600 Series System Calibration Online Training course.  Listed below are the key points that have been presented. Take time to review the material before you proceed to the final quiz.    Identify key areas of the Standard Curve screen Displays the name of the assay and the date the calibration was set. Displays the name of the reagents, lot numbers and expiration dates. Displays up to 6 points for activity or concentration Displays up to 6 points for coagulation / reaction speed for each activity or concentration. Describe the process to perform a calibration Lotnumbers and expiration dates can be manually entered or read by the optional barcode scanner The reference value of the Standard can be manually entered or read by the optional barcode scanner Verify the concentration values are updated Verify each concentration has at least 2 replicates Load reagents Press Start Press Set to accept the curve List the steps to manually enter data in a standard curve ISI and MNPT or Normalis used to calculate INR is entered manually MNPT is inputted using the keypad ISI values are on the Table of Assigned Values for the Lot number of PT reagent ISI values can be entered manually using the keypad or read by the optional handheld barcode scanner. Set to accept the new settings Select Next to continue.     Select the image areas below to learn more.  When complete, select Next to continue. Some test methods run on the analyzer need to be calibrated prior to using a new reagent lot number. The 3 tasks to calibrate a new Standard Curve are: Input lot number and expiry dates Set up and run the autodilution analysis of a standard curve Accept Standard Curve Data and perform Quality Control. When should a standard curve be performed? Learn More about when should a Standard Curve calibration be performed. When should a Standard Curve calibration be performed? When the lot number of the reagent changes for a test method When a new calculated test method is added   When test method updates or software revisions are done When problems with Quality Control analysis results occur When complete, select the X in the upper-right corner to close the window and continue.    The Standard Curve screen displays the lot numbers and expiration dates of reagents currently used by the system to calculate results. Manually entering reagent information Learn how to manually input reagent information. Checklist TitleChecklist TypeChecklist ContentSelect ReagentHTML Press the Lot No. Entry key in the standard curve screen and select the appropriate reagent. Select each checkbox to learn more about manually inputting reagent information.  Enter Lot NumberHTML Press CHG. to cycle to the numeric keys. Enter the lot number using the keypad. Press Enter when done entering the lot number.  Enter Expiration DateHTML After the lot number is entered, the Expiration Date entry screen appears with a numeric keypad. Input the expiration date using 2 digits for the month and day and 4 digits for the year-You must put a slash (/) between the month, day, and year. Press Enter when done entering the expiration date.  Update SettingsHTML After the expiration date is entered, the Renew Settings screen appears to confirm the settings are correct. Press FIX to save the lot information settings. Note: Selecting Continue will return to the lot number entry screen and start over. Selecting Cancel will quit the lot number entry screen and discard changes. When complete, select the X in the upper-right corner to close the window and continue. Entering Reagent information using barcodes Learn how to input reagent information using the optional hand-held barcode wand. Checklist TitleChecklist TypeChecklist ContentSelect ReagentHTML Press the Lot No. Entry key in the standard curve screen and select the appropriate reagent. Select each checkbox to learn more about manually inputting reagent information.  Scan BarcodeHTML Scan the barcode on the Table of Assigned Values sheet included in the box of the lot number of the reagent.   Confirm InformationHTML After you have scanned the barcode, the analyzer displays this confirmation screen. Press Enter after verifying the reagent lot number and expiration date match the information on the Table of Assigned Values.  Update SettingsHTML After the lot number and expiration date are confirmed, the Renew Settings screen appears to update the settings. Press FIX to save the lot information settings. Note: Selecting Continue will return to the lot number entry screen and start over. Selecting Cancel will quit the lot number entry screen and discard changes. When complete, select the X in the upper-right corner to close the window and continue. Autodilution calibration: Performed for most calibrated assays Uses a single standard Begins with the analyzer creating a series of dilutions of the standard Autodilution Analysis Learn how to perform an autodilution analysis. Checklist TitleChecklist TypeChecklist ContentEnter Standard Analysis data manuallySimulationInstructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90004769/sim_CA600_CalSettingsManual_9/sim_CA600_CalSettingsManual_9.swfHTML5 File:/content/generator/Course_90004769/sim_CA600_CalSettingsManual_9/index.htmlPDF File:Enter Standard Analysis data with barcode-OptionalSimulationInstructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90004769/sim_CA600_CalSettingsBarcode_9/sim_CA600_CalSettingsBarcode_9.swfHTML5 File:/content/generator/Course_90004769/sim_CA600_CalSettingsManual_9/index.htmlPDF File:Load Reagent and StandardHTML  Load all required reagents, standards and buffers.  Press StartHTML    First Tube or ContinueHTML When you select Continue, the system will  start to process the next available reaction tube.  When you select First Tube the system will use the reaction tube in the upper right corner of the reaction tube tray.   When complete, select the X in the upper-right corner to close the window and continue.     The RAPIDPoint® 400/405 Analyzer uses three replaceable cartridges for system operation. In this section, you will learn about the purpose of these cartridges and their replacement schedule. When the Standard Curve analysis is completed: The system plots the data The curve needs to be accepted QC and patient results are converted Accepting the Curve Learn how to accept a new standard curve. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90004769/sim_CA600_AcceptCurve_800x591_9/sim_CA600_AcceptCurve_800x591_9.swfHTML5 File:/content/generator/Course_90004769/sim_CA600_AcceptCurve_800x591_9/index.htmlPDF File: The Analysis screen is the system default and appears upon login. The analysis screen is used to run patient, QC, and AQC samples. Use this location to select sample type, parameters to be run, QC or AQC, or select a  panel of tests. The  ISI and MNPT or Normal-must be manually entered in the Standard Curve screen for the calculation of INR results. The ISI is obtained from the Table of Assigned Values The MNPT or Normal-is determined by your laboratory   Acronym Definition ISI ISI value for each reagent lot is used in the INR calculation to account for responsive variations between individual thromboplastins. INR The INR is used to standardize prothrombin time results regardless of the reagent system or instrument used to perform the test. The INR is calculated by using the patient's prothrombin time, the MNPT, and an ISI index supplied by the manufacturer for each lot of thromboplastin reagent. MNPT Geometric mean PT of a minimum of 100 healthy subjects and is used to calculate INR. Typically, large numbers of normal, healthy individuals are tested (100 or more). If there is a normal Gaussian distribution and the mean, mode, and median are approximately the same, then the +2SD and +3SD can be determined. Ninety-five percent of the population will be within 2SD. To check a previously determined mean, fewer normal samples (such as 20-25) may be used. Brown, Barbara B.A.,M.T.(ASCP),M.S, Hematology Principles and Procedures, Williams and Wilkins, 1993 Manually entering MNPT and ISI Learn how to manually input the ISI and MNPT. Checklist TitleChecklist TypeChecklist ContentManual EntryHTML Press the Manual Entry key in the standard curve screen.   Select each checkbox to learn more about manually inputting the ISI and MNPT.  Arrow KeysHTML Press the arrow keys to move the cursor to the Normal value.  MNPT or NormalHTML The MNPT is entered in the Normal value field. Use the keypad to enter the MNPT. Press Enter to set the value.  ISIHTML The cursor has moved into the ISI value field. Use the keypad to enter the ISI from the Table of Assigned Values for the lot of PT Reagent. Press Enter to set the value. Press Quit to exit the keypad.  Update SettingsHTML Press Set to update the new ISI and MNPT. Note: Selecting Continue will return to the lot number entry screen and start over. Selecting Quit will quit the lot number entry screen and discard changes. When complete, select the X in the upper-right corner to close the window and continue.   Entering MNPT or Normal and ISI using barcodes Learn how to input the ISI using the optional hand-held barcode wand. Checklist TitleChecklist TypeChecklist ContentManual EntryHTML Press the Manual Entry key in the standard curve screen. Select each checkbox to learn more about inputting the MNPT or Normal and ISI using barcodes.  MNPTHTML   The MNPT is entered in the Normal value field. Use the keypad to enter the MNPT or Normal. Press Enter to set the value.  Scan BarcodeHTML After you press Enter to set the MNPT or Normal, the cursor moves to the ISI value field. Scan the barcode on the Table of Assigned Values sheet included in the box of the lot number of the reagent.  Confirm InformationHTML After you scan the barcode, the analyzer displays this confirmation screen. Press Register after verifying  the ISI value matches the information on the Table of Assigned Values.  Update SettingsHTML Press Set to update the new ISI and MNPT or Normal. Note: If you select Continue, you will return to the lot number entry screen and start over. If you select Quit, you will exit the lot number entry screen and discard the changes. When complete, select the X in the upper-right corner to close the window and continue.