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Sysmex® CA-600 Series Analyzer Maintenance Online Training

Perform daily/weekly/as-needed maintenance tasks, such as replenishing consumables/reagents, LED calibration, for the efficient performance and management of the instrument. This clinical laboratory training qualifies for continuing education units (CEU).

Welcome to the Sysmex® CA-600 series Maintenance Online Training course.  In this course, you’ll learn which tasks need to be performed daily, weekly, and on an as-needed basis. Select Next to continue. Describe quarterly maintenance procedures Identify tasks required for daily maintenance Identify weekly maintenance procedures List as needed maintenance procedures Upon successful completion of this course you will be able to: Select Next to continue. Anhand der beiden Beispiele ‘Herzinsuffizienz’ und ‘Brustkrebs’ haben Sie gesehen, wie sich In-vivo- und In-vitro-Diagnostik im gesamten Versorgungskontinuum gegenseitig ergänzen können. In diesem Kapitel erfahren Sie, wie eine Probe in einem Labor bearbeitet wird. Wir werden auch die Themen „Laborautomatisierung“ und „Datenverwaltung“ etwas genauer betrachten.   Congratulations. You have completed the Sysmex® CA-600 series Maintenance online training course. Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz.   Identify tasks required for daily maintenance Perform the following tasks daily: Clean the sample probe Check and drain trap chamber fluid Discard used reaction tubes Remove condensation from the reagent rack Dispose of waste Check and record temperatures Check and replace the following as needed: Rinse solution Reaction tubes CA Clean I, CA Clean II and buffer Other reagents Identify weekly maintenance Weekly maintenance includes cleaning the instrument interior and exterior with water and neutral detergent. When you are cleaning dried reagent or sample spills: Clean with 1:2 diluted CA Clean I Wipe with a towel moistened with water Dry with a soft towel   Describe quarterly maintenance procedures Quarterly maintenance includes: Cleaning the rinse bottle Performing an LED Calibration Perform an LED calibration: Every 90 days, or Every 30 days if your laboratory reports a direct fibrinogen result, which is sensitive to changes in scattered light intensity.   List as needed maintenance procedures Replace fuses Replace printer paper Perform Rinse & Prepare Select  Next to continue.   Ein Arzt sendet den Anforderungsschein für diverse klinisch-chemische Untersuchungen an das Labor. Blut wird entnommen und die Probe ins Labor transportiert. Nach der Zuordnung der jeweiligen Probe zur Anforderung hilft eine Reihe automatisierter Prozesse, eine gleichbleibend hohe Qualität und effiziente Arbeitsabläufe zu erreichen. Die Probe wird automatisch zentrifugiert, um das Blut zur weiteren Untersuchung vorzubereiten. Um die Laborsicherheit und die Probenbearbeitungszeit zu verbessern, werden die Verschlüsse aller Probenröhrchen automatisch entfernt. Von der Probenverwaltungseinheit, die die Proben aufbewahrt, werden diese automatisch auf ein Fließband gestellt und zum angegebenen System transportiert. Dort werden die notwendigen Mengen der Probe durch einen Pipettierroboter entnommen, wodurch genaueste Probenvolumina sichergestellt werden. Die einzelnen klinisch-chemischen Testverfahren werden natürlich ebenfalls automatisch durchgeführt. Sobald die analysierten Daten bestätigt sind, werden sie dem anfordernden Arzt zugeschickt, während das Probenröhrchen an den Aufbewahrungsort transportiert wird.   To ensure proper performance of your Sysmex® CA-600 series coagulation analyzer, perform the daily maintenance tasks shown below. Be sure to keep a record of all maintenance performed. Perform the following tasks daily: Clean the sample probe Check and drain trap chamber fluid Discard used reaction tubes Remove condensation from the reagent rack Dispose of waste Check and record temperatures  Check and replace the following as needed: Rinse solution Reaction tubes CA Clean I, CA Clean II and buffer Other reagents Download and print a copy of the Sysmex® CA-600 Series Maintenance checklist. Perform the following tasks daily: Clean the sample probe Check and drain trap chamber fluid Discard used reaction tubes Remove condensation from the reagent rack Dispose of waste Check and record temperatures Cleaning the Sample Probe Learn about cleaning the sample probe. Checklist TitleChecklist TypeChecklist ContentClean the probe exteriorHTML   If media does not automatically start, select the play arrow to begin. Select each checkbox to learn more about cleaning the sample probe. Verify cleaners are setHTML Verify that CA Clean I is set in position 11 and contains sufficient volume. Verify that CA Clean II is set in position 13 and contains sufficient volume.   Warning: CA CLEAN II is an acidic cleaning agent. It should not come in contact with skin or clothing. If it happens rinse skin or clothing with plenty of water to avoid injury or damage. CA CLEAN I contains sodium hypochlorite. Be sure to use CA CLEAN I and CA CLEAN II in different containers. If CA CLEAN I is directly mixed with acid solution such as CA CLEAN II, chlorine gas is produced, which is very dangerous. Execute Probe RinseSimulationInstructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90004736/sim_CA600_RinseProbe_800x591/sim_CA600_RinseProbe_800x591/sim_CA600_RinseProbe_800x591.swfHTML5 File:/content/generator/Course_90004736/sim_CA600_RinseProbe_HTML5_700x517/index.htmlPDF File: Check and Drain the Trap Chamber Fluid Learn how to check and drain the trap chamber fluid.   If media does not automatically start, select the play arrow to begin. When complete, select the X in the upper-right corner to close the window and continue. Warning: When draining the trap chamber, always wear latex or non latex examination gloves. After completion of work, wash hands with disinfectant. Handle all instrument parts as biologically hazardous. There is a risk of infection with pathogens etc.. Other Daily Tasks Learn about other daily maintenance tasks. Checklist TitleChecklist TypeChecklist ContentEmpty reaction tube trash drawerHTML The Sysmex® CA-600 series coagulation analyzer automatically deposits used reaction tubes into the tube trash drawer, which holds approximately 60 used reaction tubes. The system does not keep track of the reaction tubes collected.  The drawer must be checked regularly and emptied when necessary. Clean the drawer with tap water, dry, and then replace the drawer into the instrument. Warning: When discarding used reaction tubes, always wear latex or non latex examination gloves. After completion of work, wash hands with disinfectant. Handle all instrument parts as biologically hazardous. There is a risk of infection with pathogens etc. Also, medical waste and infectious waste should be properly disposed of. Select each checkbox to learn more about the daily tasks. Remove dew from the reagent rackHTML   If media does not automatically start, select the play arrow to begin. Dispose of wasteHTML   If media does not automatically start, select the play arrow to begin. Warning: When you dispose of waste, always wear latex or non latex examination gloves. After completion of work, wash your hands with disinfectant. Handle all instrument parts as biologically hazardous. There is a risk of infection with pathogens etc. Also, medical waste and infectious waste should be properly disposed of. Be careful that no waste is splashed. If it is splashed, wear latex gloves and wipe the area with gauze or a towel moistened with 1:2 diluted CA CLEAN I, then wipe with a towel moistened with water, and finally wipe with a dry soft towel. Check and record temperaturesSimulationInstructions:If media does not automatically start, select the play arrow to begin. When complete, select the X in the upper-right corner to close the window and continue.Flash File:/content/generator/Course_90004736/sim_CA600_CheckTemp_800x591_9/sim_CA600_CheckTemp_800x591_9.swfHTML5 File:/content/generator/Course_90004736/sim_CA600_CheckTemp_800x591_9/index.htmlPDF File: The ADVIA Chemistry XPT System has several significant components. These can be separated into the following areas: Top view, Front View and Rear View. The Top View includes the Sample Handling Area, the Spectrophotometer and Reaction Tray, Reagent Handling Area, and the ISE module where electrolytes are measured.    The Front View includes the Ancillary Reagents, the power panel and the system workstation.   Finally, the Rear View is where the Main power switch, waste and external water supply can be accessed. Select Next to continue.   Check and replace the following as needed: Rinse solution Reaction tubes Cleaners, diluent and reagents Replenish the Rinse Solution Learn how to replenish the rinse solution. Checklist TitleChecklist TypeChecklist ContentReplenish rinse prior to analysisHTML   If the media does not automatically start, select the play arrow to begin.   Select each checkbox to learn more about replenishing the rinse solution. Replenish rinse during analysisHTML When rinse solution becomes insufficient during analysis, the confirmation screen will appear with the error message. Press the Conf. key.   The alarm stops and “Waiting” screen will appear. When analysis of the samples being incubated is complete, the Analysis Start Confirmation screen appears. Wait until the system status becomes “Ready” and replenish the rinse solution in the external container. Press Start key or Cancel key on the Analysis Start Confirmation screen. Start key: Starts analysis after interruption. Cancel key: Interrupts analysis and returns the screen to the Main Menu. When complete, select the X in the upper-right corner to close the window and continue. Refilling Reaction Tubes Learn how to refill reaction tubes. Checklist TitleChecklist TypeChecklist ContentRefill reaction tubesHTML Check the reaction tube racks and replenish with unused tubes, whenever necessary. Use only Sysmex SU-40 reaction tubes.  Do not reuse reaction tubes as this may impact patient results.    Select each checkbox to learn more about refilling reaction tubes.  Select First Tube when beginning analysisHTML Remember to select First Tube upon starting processing samples when both trays are completely filled.  This will tell the system to start with the reaction tube in position number one on the tray.  The system software will then monitor the availability of reaction tubes in the trays with a software counter.  The system will alarm is insufficient reaction tubes are available at the beginning of analysis.     When complete, select the X in the upper-right corner to close the window and continue. Replacing Cleaners, Diluent and Reagents Learn how to replace the cleaners, diluent and reagents. Checklist TitleChecklist TypeChecklist ContentReplacing cleanersHTML The Sysmex CA-600 series analyzer requires 2 distinct cleaners onboard at all times.   CA Clean I Loaded into position 11 1% sodium hypochlorite solution Used for cleaning during sample analysis CA Clean II Loaded into position 13 Hydrochloric acid solution Used during the maintenance rinse and for specific assays  Both cleaners should be replaced every 24 hours using a fresh container.  Do not top off the existing container to avoid contamination and to maintain reagent stability and strength.   Select each checkbox to learn more about replacing the cleaners, diluents and reagents. Replacing diluentHTML The Sysmex CA-600 series analyzer uses Owren’s Veronal Buffer (OVB) as a diluent for dilutions on specific assays and while performing standard curve calibrations.  The OVB should be replaced every 8 hours using a fresh container in position 12.  The buffer should be allowed to come to room temperature before loading it onboard the instrument.  Do not top off the existing container to avoid contamination and maintain reagent integrity.  Replacing other reagentsHTML Each test method performed on the Sysmex® CA-600 series coagulation analyzer requires a specific set of reagents.  On a daily basis, verify that the reagents you are loading match the reagent information that is in the system.  Each reagent has a unique onboard stability.  Refer to the specific assay’s application sheet for the onboard stability information.  Reagents can be loaded in a GW5 vial SLD vial, or a 4mL cup.  Reagents loaded in a 4mL cup require the use of holder #89.  Register reagent volumes (if applicable)SimulationInstructions:If the media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90004736/sim_CA600_RegisterRgntVol_800x591_9/sim_CA600_RegisterRgntVol_800x591_9.swfHTML5 File:/content/generator/Course_90004736/sim_CA600_RegisterRgntVol_800x591_9/index.htmlPDF File: The spectrophotometer measures the amount of light absorbed by the sample reagent mixture contained in the reaction cuvettes using 14 specific wavelengths of light generated by a halogen lamp.  Select the link below to learn about the location and function of the Spectrophotometer and RRV areas. audf_AXPT_HardwareOverview_SpecRRV.mp3 Cleaning the instrument interior and exterior with water and neutral detergent. When you are cleaning dried reagent or sample spills: Clean with 1:2 diluted CA Clean I Wipe with a towel moistened with water Dry with a soft towel Warning: When you are cleaning the instrument, always wear latex or non latex examination gloves. After completion of work, wash your hands with disinfectant. Handle all instrument parts as biologically hazardous. There is a risk of infection with pathogens etc.  Select Next to continue.   Quarterly maintenance includes: Cleaning the rinse bottle Performing an LED Calibration Perform an LED calibration: Every 90 days, or Every 30 days if your laboratory reports a direct fibrinogen result, which is sensitive to changes in scattered light intensity. LED Calibration Procedure Learn about the LED Calibration procedure. Slide NumberText BlocksCalloutsAudio ScriptImage File1LED Calibration: Optimizes LED performance Improves the accuracy of results Is performed every 30 for laboratories reporting a derived fibrinogen result Is performed every 90 days for all other laboratories   Select Next to continue.   Note: If audio does not automatically start, select the play arrow in the top left to begin.The Sysmex CA-600 Series analyzer LED calibration optimizes the LED performance and improves the accuracy of results. The LED calibration must be performed every 30 days if your laboratory reports a derived fibrinogen result, which is sensitive to changes in scattered light intensity. If a derived fibrinogen is not reported, the LED calibration must be performed every 90 days. After the analyzer power is cycled, the LED calibration confirmation screen will appear when the calibration is due. Select next to continue. 2The CA CAL S Calibrator: Is a latex suspension used to calibrate the optical system Is packaged 3 bottles per pack and is ready to use Must be refrigerated between 2-8°C Any unused portion must be discarded Each lot is assayed with a target value   Select Next to continue. The LED Calibration uses CA CAL S, a latex suspension to calibrate the optical system. The CA CAL S is packaged three bottles per pack and is ready to use. The CA CAL S product must be refrigerated and once opened, it should be used in 1 day. The CA CAL S has a target value and an expiration date for each lot number of calibrator. Select next to continue. 3LED Calibration Procedure:   Step 1:  Press OK on the LED calibration confirmation screen.When the LED calibration confirmation screen appears, press OK4 LED Calibration Procedure:   Step 2:  Navigate to the LED Calibration Menu screen by pressing Special Menu > Special Operate.  On the LED Calibration Menu screen press the Calibration key to access the LED calibration screen.   Select Next to continue.Navigate to the LED Calibration Menu screen by pressing Special Menu > Special Operate. On the LED Calibration Menu screen press the Calibration key to access the LED calibration screen. Select Next to continue.5LED Calibration Procedure:   Step 3:  Identify the CA CAL S Target Value.   Select Next to continue.Next, refer to the CA CAL S package insert sheet to obtain the correct Target Value. This value is specific to the lot number of CA CALS calibrator used. Select next to continue.6LED Calibration Procedure:   Step 4:  Enter the Target Value.   Select Next to continue.Using the key pad, enter the target value on the LED Calibration screen. In this example, the target value is 883. Select next to continue.7LED Calibration Procedure: Step 5: Set the Vial Type. Select Next to continue. Using the arrow keys to move the cursor on the LED Calibration screen to the Vial type. Select the vial used to set the calibrator in the reagent holder. In this example, the calibrator will be loaded into a 4mL cup. Select next to continue.8LED Calibration Procedure:   Step 6:  Load calibrator.   Select Next to continue.For this example, a 4mL sample cup was selected to hold the calibrator. Add 1550 microliters of CA CAL S to the cup and place the calibrator into the reagent holder position number 1. Also set CA Clean I in a PV 10 vial into reagent holder position number 11. Select Next to continue.9LED Calibration Procedure:   Step 7:  Initiate calibration.   Select Next to continue.Close the light shield and press Set on the LED Calibration screen to initiate the LED calibration. Select Next to continue.10LED Calibration Procedure:   Step 8:  Reviewing and Accepting the calibration   LED Availability Status: OK       The LED is available for use. *OK     The LED is weak, but still available for use ERRx   The LED is not available for use.  Once the calibration is accepted, this LED will not be used for analysis. When complete, select the X in the upper-right corner to close the window and continue.Upon completion of the LED Calibration sequence, the LED Calibration Update Confirmation screen appears. This screen includes each channel number and the channel’s status. LED status availability messages also display. Press FIX to accept the calibration. Any LED’s out of tolerance will not be used for analysis. To reject the calibration, press Cancel. Cleaning the Rinse Bottle Learn about cleaning the rinse bottle. When complete, select the X in the upper-right corner to close the window and continue.   On a quarterly basis, the rinse bottle must be decontaminated.  To clean the rinse bottle, perform the following: Remove the rinse bottle cap by turning it counterclockwise Empty the contents of the bottle Add a sufficient amount of 70% isopropyl alcohol to coat the interior surfaces of the bottle Shake the bottle to coat the interior surfaces Allow to sit for 10 minutes and empty Rinse the bottle with fresh deionized water Refill the bottle and reassemble NOTE:  When reattaching the bottle, make sure the cap is securely tightened.   To ensure proper performance of your Sysmex® CA-600 series analyzer, perform the as-needed maintenance tasks below. Be sure to keep a record of all maintenance performed. Replace fuses Replace printer paper Perform Rinse & Prepare Replacing Fuses Learn about replacing fuses. Overcurrent protection fuses are housed on the rear panel. When fuses are blown, replace them. Important:  To avoid risk of electric shock, disconnect the power cord before replacing fuses. For continued protection against risk of fire, replace only with 250v 4.0A slow blow fuses.   When complete, select the X in the upper-right corner to close the window and continue.   Replace Paper Learn how to replace the printer paper. Checklist TitleChecklist TypeChecklist ContentRemove the printer coverHTML Remove the printer cover by lifting the lower edge and detach it.   Select each checkbox to learn more about replacing the printer paper.  Release the paper rollHTML Raise the lock lever located on the upper right corner to release and remove the existing paper roll.  Load new paper rollHTML Load the paper roll with the flap toward the inside of the paper compartment as shown. Always use Siemens thermal printer paper.  Route the paperHTML Insert the paper under the roller and pull out as indicated.  Lower the lock lever and replace the cover.  Perform Paper FeedHTML Press the Sysmex key to access the Sysmex menu screen Press P. FEED to advance the paper.   When complete, select the X in the upper-right corner to close the window and continue.  Rinse and Prepare Learn about performing a rinse and prepare. Checklist TitleChecklist TypeChecklist ContentPress Special MenuHTML Prime rinse solution to the hydraulic lines weekly or whenever the instrument has been left idle for a long period.  From the Main Menu screen, press Special Menu.     Select each checkbox to learn more about the rinse and repair operation. Press Special OperateHTML On the submenu, press, Special Operate.    Press Rinse and PrepareHTML On the Special Operation Menu screen, press Rinse & Prepare.  Press SetHTML Press Set.  Rinse BeginsHTML The rinse will begin.   When complete, select the X in the upper-right corner to close the window and continue.