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Sysmex® CA-600 Series Analyzer Reagent Setup Online Training

Accurately identifying reagents and loading them properly on the Sysmex® CA-600 Series System is a critical step in providing accurate patient results. This clinical laboratory training qualifies for continuing education units (CEU).

Welcome to the Sysmex® CA-600 Series Coagulation Analyzer Reagent Setup Online Training course. Reagent parameters that must be defined include: Reagent name Lot number Vial type Reagent position   Select Next to continue. Identify reagent positions List daily reagent setup tasks Locate reagent information Recognize the Siemens reagent vial types Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations. You have completed the Sysmex® CA-600 Series Analyzer Reagent Setup online training course. Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz.  Locate reagent information     Reagent Package Insert System Application Sheets Tables of Analytical Values Location Inside the reagent packaging or on the Siemens website: www.siemens.com/diagnostics Provided by Siemens Application Specialist during installation or on the Siemens website: www.siemens.com/diagnostics Inside the reagent packaging Contents Reconstitution instructions Storage and Handling instructions Test methodology Required reagents for an assay and appropriate positions Onboard stability Calibration information Lot specific reference values (such as the ISI or calibrator values) Important Notes Package inserts are the same for a specific reagent regardless of the reagent lot. Application sheets are for a specific assay on a specific analyzer. The Tables of Analytical Values can change for each new lot of reagent. Identify Siemens reagent vial types 4mL cup (Cup) Siemens 5mL vial (GW5) Push Vial (PV-10) Sysmex Low Dead Volume vial (SLD)   Identify reagent positions Cooled Reagent Holders:  Can hold up to 4 vials at 15°C GW5, PV-10 or SLD vials can be set directly 4mL cups require the insertion of a reagent holder Room Temperature Reagent Holders:   Can hold up to 6 vials at room temperature GW5, PV-10 or SLD vials can be set directly 4mL cups require the insertion of a reagent holder CA CLEAN I: CA CLEAN I must be loaded in reagent holder 11 GW5, PV-10 or SLD vials can be set directly 4mL cups require the insertion of a reagent holder CA CLEAN II: CA CLEAN II must be loaded in reagent holder 13 4mL cups can be set directly No other vial types are appropriate Buffer: OVB must be loaded in reagent holder 12 if chromogenic, immunologic, or assays requiring buffer are performed GW5, PV-10 or SLD vials can be set directly 4mL cups require the insertion of a reagent holder List daily reagent setup tasks Verify reagent information The Standard Curve screen displays reagent information currently used by the system To access the standard curve screen: From the Main Menu screen, press Standard Curve. Press Select Test to view the reagent information for the selected test. Prepare reagents Strictly follow the instructions as given in the package insert supplied with each reagent.  Otherwise, you will fail to obtain correct analysis results. Raise light shield Check that the analysis status display is “Ready,” and open the light shield cover. WARNING:  Be sure to fully open the light shield cover and check that it will not fall down by its own weight.  If the light shield cover falls down, there is a risk of causing injury. Load reagents GW5, PV-10 and SLD vials or 4mL cups with reagent holder adapters can be set into the reagent rack.           NOTE: Always set the reagents at the specified positions Confirm that all reagents contain no bubbles Be sure to set CA CLEAN I in Holder No. 11 Be sure to set CA CLEAN II in Holder No. 13 Register reagent volumes If the Reagent Monitoring feature is enabled in your laboratory, it is important to register the reagent volume whenever the reagent is replaced.  Proceed as follows: From the Main Menu, Press Special Menu Press Set Reagents Press the key of the reagent holder to be registered Enter the reagent volume set in the reagent holder Press Enter Press Quit Press Main Menu Press FIX to update the settings Select Next to continue.     Reagent Package Insert System Application Sheets Tables of Analytical Values Location Inside the reagent packaging Provided by Siemens Application Specialist during installation or on the Siemens website: www.siemens.com/diagnostics Inside the reagent packaging Contents Reconstitution instructions Storage and Handling instructions Test methodology Required reagents for an assay and appropriate positions Onboard stability Calibration information Lot specific reference values (such as the ISI or calibrator values) Important Notes Package inserts are the same for a specific reagent regardless of the reagent lot. Application sheets are for a specific assay on a specific analyzer. The Tables of Analytical Values can change for each new lot of reagent. Select Next to continue. Select each tab below to learn about the different vial types. Full Name Sample cup conical 4mL Abbreviation in software Cup Total Volume 4mL Dead Volume Approximately 0.2mL   Full Name GW5 Abbreviation in software GW5 Total Volume 5mL Dead Volume Approximately 0.8mL   Full Name Push Vial PV-10 Abbreviation in software PV-10 Total Volume 10mL Dead Volume Approximately 0.9mL   Full Name Sysmex low dead volume vial Abbreviation in software SLD Vial Total Volume 5mL Dead Volume Approximately 0.4mL   Select the image areas below to learn more. Perform the following reagent setup tasks daily: Verify reagent information Prepare reagents Raise light shield Load reagents Register reagent volumes Daily Reagent Setup Tasks Learn about each of the daily reagent setup tasks. Checklist TitleChecklist TypeChecklist ContentVerify reagent informationHTML The Standard Curve screen displays reagent information currently used by the system. Before loading reagents, verify that the reagents, lot numbers, and expiration dates match the reagent information displayed on the Standard Curve screen to ensure that you are using the same reagents that the system uses to calculate final test results. To open the Standard Curve screen, from the Main Menu screen, press Standard Curve. On the Standard Curve screen, press Select Test to view the reagent information for the selected test.  Select each checkbox to learn more about daily reagent setup tasks.   Prepare reagentsHTML Prepare reagents as per the reagent package insert.   WARNING:  Strictly follow the instructions as given in the package insert supplied with each reagent.  Otherwise, you will fail to obtain correct analysis results.    Raise light shieldHTML Check that the analysis status display is “Ready,” and open the light shield cover.   WARNING:  Be sure to fully open the light shield cover and check that it will not fall down by its own weight.  If the light shield cover falls down, there is a risk of causing injury.  Load reagentsHTML GW5, PV-10 and SLD vials or 4mL cups with reagent holder adapters can be set into the reagent rack. NOTE: Always set the reagents at the specified positions Confirm that all reagents contain no bubbles Be sure to set CA CLEAN I in Holder No. 11 Be sure to set CA CLEAN II in Holder No. 13  Register reagent volumes (if applicable)SimulationInstructions:If media does not automatically start, select the play arrow to begin. When complete, select the X in the upper-right corner to close the window and continue.Flash File:/content/generator/Course_90004654/sim_CA600_RegisterRgntVol__9/sim_CA600_RegisterRgntVol__9.swfHTML5 File:/content/generator/Course_90004654/sim_CA600_RegisterRgntVol__9/index.htmlPDF File: Test Groups Learn more about reagent test groups. When complete, select the X in the upper-right corner to close the window and continue.   How does the Sysmex® CA-600 series coagulation analyzer know what to which positions and vials the reagents are assigned? During instrument setup or as needed, your Siemens Technical Application Specialist (TAS) will perform the following reagent setup tasks to initially define reagent information to the system: Set up test groups A test group is a pre-defined group of tests that are displayed on the worklist as you load samples and order tests for each sample. Your TAS will define three test group configurations to suit the needs of your laboratory. For example, Test Group 1 could include PT, APTT, Fbg, and D-Dimer.   Set reagent holder positions and vial types   Each reagent holder position must be assigned to a specific reagent and vial type so that the system knows which reagent to aspirate and which automatic probe height adjustment to implement when the probe aspirates reagent from varying vial sizes Review  the following frequently asked questions for help locating the Siemens reagent information you need.   Question Answer What is a test group? A test group is a group of up to five tests that are grouped together. Three test group configurations are available. Tests in the selected test group appear on the worklist so that you can order them as you register sample order information. To view an alternative test group on the worklist, from Main Menu, press Test Group. Select the test group that you want to appear on the worklist. Where do I find the reagent lot number currently used on the instrument? The Standard Curve screen displays reagent information currently used by the system to calculate patient and QC results, including reagent names, lot numbers, and expiration dates. Always verify that the reagents you are loading match the reagent information displayed on the Standard Curve screen to ensure that you are using the same reagents that the system uses to calculate final test results. To open the Standard Curve screen: From the Main Menu screen, press Standard Curve On the Standard Curve screen, press Select Test to view the reagent information for the selected test. Where do I find the lot number for a reagent? Reagent lot numbers and expiration dates are listed on the outside of the box that they are packaged in. This information is also listed on the side of the reagent vial. The lot number is a six-digit number. Where can I find the correct reagent holder position and vial type to load a reagent? Use the Reagent Holder Setting screen to view reagent holder and vial type information. To open the Reagent Holder Setting screen From the Main Menu screen, press: Special Menu—Settings—Analysis Settings—Reagents Holder The reagent holder positions and vial types are specific for each test group. To view reagent holder and vial type information for another test group, on the Reagent Holder Setting screen, press Change Group. Where can I find how to reconstitute a reagent? The Package Insert Sheet that comes in each box of the reagent explains how to reconstitute the reagent and how much diluent to use. Reagent stability is also listed. For onboard reagent stability, please refer to Application Sheet for the specific test. Where can I find how long a reagent is stable once reconstituted? The Package Insert Sheet that comes in each box of reagent lists the stability for a closed reagent vial. The Application Sheet for the specific test lists the onboard stability of the reconstituted reagent, which is the stability that must be followed for reagents loaded onto the instrument. Where can I find assay values for calibrators? The assay value for Standard Human Plasma (SHP) calibrator is listed on the Table of Analytical Values insert sheet found inside each box of SHP. The assay value for  Innovance® D-Dimer calibrator is listed on the Table of Analytical Values insert sheet found inside each box of Innovance® D-Dimer Assay kit. The lot number and expiration date are also found on the side of the vial. Note:  Immunologic test methods, such as D-Dimer, are available only on Sysmex® CA-660 coagulation analyzers. Where can I find the MNPT and ISI values used to calculate PT Ratio and INR? Two Package Insert Sheets are found in boxes of PT reagent (Dade® Innovin® Reagent, and Thromborelâ S Reagent). One sheet provides information about the reagent, such as reconstitution information and closed vial stability. The other sheet lists a table of ISI values for several Siemens instruments, as well as a few other instruments. Be sure to obtain the lot-specific ISI value that is specific to the instrument you are using to calculate the INR! The mean normal PT.(MNPT) is determined by each lab by running normal samples and calculating MNPT. What information can I find on an Application Sheet? Application Sheets display the following:   Reagents needed to run each test method Quality Control products Calibrator used to run a calibration Onboard position and stability of reconstituted reagents Dilution set and to define calibrator dilution ratios and number of replicates to perform Test Protocol settings that define how the instrument runs each test method Where can I obtain Application Sheets for each test method? Your CAS can supply Application Sheets to you. You should have an Application Sheet for each test you run on your Sysmex® CA-600 series coagulation analyzer. Where can I find more information about my Sysmex® CA-600 series coagulation analyzer? Refer to your Operator’s Manual for your Sysmex® CA-600 series coagulation analyzer. Select Next to continue.