ADVIA® Chemistry XPT System Hardware Overview Online Training

Welcome to the ADVIA® Chemistry XPT System Hardware Overview Online Training course. This course will introduce you to the main components of the ADVIA® Chemistry XPT System, their location and their functions. Select Next to continue. Describe the functions of the main system components Identify hazard labels Locate the system components Upon successful completion of this course, you will be able to: Select Next to continue. Congratulations.  You have completed the ADVIA® Chemistry XPT System hardware overview online training course.  Listed below are the key points that have been presented.  Take time to review the material before you proceed to the final quiz.. In this online training course, you have learned to: Identify hazard labels Biohazard, Electrical, Laser,General, Sharps and Temperature Locate the system components Top- Sample Handling Area, Reagent Handling Area, ISE Module, Spectrophotometer and Reaction Tray Area(RRV) Front- Ancillary Reagents Area, Power Panel, and Workstation (Mouse, Monitor, Printer, Keyboard, CPU) Rear - Main Power Switch, External Water Supply and Waste Area Describe the function of the main system components Sample Handling Area: Sample Tray (STT):  Holds patient samples and multi point calibrators at room temperature for aspiration by the sample dilution probe. It is equipped with a barcode reader to identify samples. Control/Calibrator Tray (CTT):  Refrigerated tray of single point calibrators, controls, special purpose diluents, water and detergents. Sample Dilution Probe (DPP):  Aspirates and dispenses samples, along with saline, into the Dilution Tray (DTT) making a 1:5 dilution. It also aspirates and dispenses undiluted samples into the ISE module. Dilution Tray (DTT):  Contains 120 cuvettes at room temperature that hold 1:5 diluted samples for aspiration by the Sample Probe. Dilution Mixer (DMIX):  Mixes each sample and saline in the DTT cuvettes. Sample Probe (SPP):  Aspirates and dispenses individual aliquots of sample for each assay into the Reaction Tray (RRV) cuvettes. Dilution Tray Wash Unit (DWUD): Cleans the DTT cuvettes with DI H2O. ISE Module: Contains disposable electrodes to measure electrolytes including Sodium (Na), Potassium (K), and Chloride (Cl). Reagent Handling Area: Reagent Trays (RTT1 & RTT2):  Trays are refrigerated and hold primary reagents for assay analysis. Reagent Probes (RPP 1 & RPP2):  Aspirate and dispense primary reagents for analysis from their respective reagent trays. Reaction Mixers (MIX-1 & MIX-2):  Mixes samples with reagents in the RRV cuvettes, as needed. Barcode Reader:  Reagent trays are equipped with a barcode scanner to identify reagents present in the trays. Spectrophotometer and RRV Area: Spectrophotometer:  Measures the absorbance of each reaction mixture in the RRV cuvettes. Halogen Lamp:  Generates the light absorbed by the reactions. Cooling Tank:  Holds the fluid that maintains lamp temperature. Reaction Tray (RRV):  Holds the reaction mixtures for each assay in cuvettes maintained at 37º C with an oil bath. Reaction Tray Wash Unit (WUD):  Dispenses and aspirates deionized water and cleaning solutions to clean RRV cuvettes after each use. System Pumps: Activates mechanisms for aspirating and dispensing fluids. Ancillary Reagent Area contains the following: ISE buffer, Incubation Oil, Isotonic Saline, Cuvette Wash solution, Cuvette Conditioner and Pure Water bottle. The Main Power switch: Power up or shuts down the system to execute a hard reboot. The Waste containers: Collects waste from different areas of the system.  Work Station area components are used to navigate the software, operate the system and print when needed. Select Next to continue.     Observe laboratory safety procedures by being aware of safety symbols.  The safety symbols below appear on the interior and exterior of the ADVIA® Chemistry XPT system.  Note: Refer to the ADVIA® Chemistry XPT Operator's Guide or Online Help for further details. Biohazard Label The biohazard label indicates a risk for biohazard. Biohazard labels are placed on the sample dilution probes since these probes are exposed to the sample during the analysis. Electrical Hazard Label The electrical hazard label indicates that there is a risk of an electrical hazard.   Laser Hazard Label The laser hazard label indicates that there is a risk of exposure to lasers.  Laser barcode readers are used in the following sampling mechanisms: Sample Tray (STT) Reagent Tray 1 (RTT1) Reagent Tray 2 (RTT2)   General Hazard Label The general hazard label is used both as a warning and a caution. Warning: Indicates the risk of personal injury. The wash solutions and some method reagents are classified as hazardous materials. They can be harmful if swallowed or come into contact with the eyes or skin. Caution: Indicates the possibility of damage to or destruction of equipment. Incorrect results could occur if the system is not used in the proper manner as indicated in the Operator Guide. Sharp or Pinch Label This symbol indicates a moving component, such as a probe, that can cause injury.   Temperature The temperature label indicates the presence of a part emitting a high temperature.  A high temperature label will be found near the halogen lamp. The system components of the ADVIA® Chemistry XPT System can be divided into the following categories: Top- Sample Handling Area, Reagent Handling Area, ISE Module, Spectrophotometer and Reaction Tray Area(RRV) Front- Ancillary Reagents Area, Power Panel, and Workstation (Mouse, Monitor, Printer, Keyboard, CPU) Rear - Main Power Switch, External Water Supply and Waste Area Select Next to continue.   There are three main areas accessible from the top of the analyzer: Sample Handling area including the ISE module Reagent Handling area Spectrophotometer and RRV area ADVIA XPT Top View Learn about the system components from the top view. Base ImageHotspotsText BlocksImage File The Sample Handling Area and ISE module area: The Sample Handling areas consists of the Sample Tray Turntable (STT), Dilution Tray Turntable (DTT) and the Calibration and Control Tray Turntable (CTT). The Reagent Handling Area: Reagent Tray 1 (RTT1) has 60 refrigerated positions. 1-56 are used for reagents and 57-60 are used for wash detergents that prevent contamination.  Reagent Tray 2 (RTT2) has 56 refrigerated positions. 1-52 are used for reagents and 53-56 for wash detergents that prevent contamination.  The Spectrophotometer and the RRV Area: The RRV moves reaction vessels in front of the spectrophotometer every 13.5 seconds to determine concentration levels.   The Sample Handling area is the site where samples are first introduced to the system for analysis. The Sample Handling Area consists of the following components: Sample Tray (STT) Calibrator/ Control Tray (CTT) Dilution Tray (DTT) Sample Probe (SPP) Dilution Probe (DPP) Dilution Tray Mixer (DMIX) ISE Module Note: In most cases the system will be attached to an automation system. Sample Handling Area Learn about the sample handling area. Slide NumberText BlocksCalloutsAudio ScriptImage File1 The Sample Tray (STT) The STT holds patient samples and multipoint calibrators for analysis Consists of 2 rows each with 42 positions for a total of 84 positions Accommodates 5 mL, 7 mL, and 10 mL tubes, 1 mL and 2 mL sample cups and  user-defined containers Holds serum, plasma, urine, whole blood and CSF (assay dependent) in each position Equipped with a barcode reader Maintained at room temperature  Select Next to continue.   Note: If audio does not automatically start, select the play arrow in the top left to begin.The STT holds patient samples for analysis. It consists of 2 rows, each with 42 positions, for a total of 84 positions. In each position, serum, plasma, urine, whole blood and CSF, based on assy in 5 mL, 7 mL, and 10 mL tubes, or 1 mL and 2 mL sample cups and user-defined containers can be used. This is also where multipoint calibrators are loaded on the instrument. It is equipped with a barcode reader for positive sample identification. The STT is maintained at room temperature. Select next to continue. 2 The Calibrator/Control Tray (CTT) Holds single point calibrators, controls and special purpose diluents The outer row contains 34 positions The inner row contains 27 positions Accommodates 10, 7, and 5 milliliter collection tubes The inner wheel holds the maintenance solutions The CTT is kept at 6 to 14° C   Select Next to continue.  The CTT is used to hold single point calibrators, controls and special purpose diluents. It consists of an outer row with 34 positions and an inner row which contains 27 positions for a total of 61positions. The CTT also holds water and detergents for maintenance purposes. The temperature of the CTT is maintained between 6 and 14 degrees Centigrade. Select next to continue. 3 The Sample Dilution Probe (DPP) The DPP aspirates samples from the STT, CTT, or a laboratory automation system, and dispenses the samples into cuvettes in the DTT or ISE module, according to the specified assay requirements. Select Next to continue.  Select each number to review the corresponding text.Callouts Sample Dilution Probe (DPP) Dilution Tray (DTT) The DPP aspirates samples from the STT or CTT from the system, or a Laboratory Automation System. It dispenses the samples into cuvettes in the DTT according to the specified assay parameters. The DPP also pipettes samples directly into the ISE for Electrolyte analysis. Select next to continue. 4 The Dilution Tray (DTT) and Dilution Tray Mixer (DMIX) The DTT wheel contains 120 reusable cuvettes. Maximum sample volume for each cuvette is 300 uL. DPP dispenses sample into cuvettes in the DTT, making a 1:5 dilution with saline. The sample is stirred by the DMIX, aspirated by the SPP and dispensed into the RRV. The Reaction Tray Wash Unit (WUD) and the Dilution Tray Wash Unit (DWUD) wash the cuvettes after analysis. Select Next to continue.   Select each number to review the corresponding text.Callouts Dilution Tray (DTT) Dilution Tray Mixer (DMIX) Dilution Tray Wash Unit (DWUD) Sample Probe (SPP) The cuvettes in the DTT but are kept at room temperature. The dilution tray contains 120 reusable cuvettes with a maximum sample volume of 300 microliters each. The DPP dispenses samples into cuvettes in the DTT making a 1 to 5 dilution with saline. Once a sample has been transferred into a cuvette it is stirred by the DMIX and then aspirated by the SPP which dispenses the sample into the reaction tray, or RRV, for incubation and absorbance reading. After each analysis the DTT cuvettes are washed with water by the dilution tray wash units, DWUD, and the RRV cuvettes are washed by the reaction tray wash units, or WUD, which uses water and detergents. 5 ISE Module The ISE module is used to measure three electrolytes in serum, plasma or urine samples: Sodium (Na+) Potassium (K+) Chloride (Cl-)    When complete, select the X in the upper-right corner to close the window and continue. The ISE module determines the amount of sodium, potassium and chloride in serum or urine samples using voltage measurement by ion-selective electrodes in a two-stage process. The DPP aspirates directly from the sample tube on the STT for ISE measurement. The sample is delivered into the ISE dilution bowl and a 1 to 33 dilution is made using ISE buffer. Select the X in the upper-right corner to close the window and continue.   The Reagent Handling area consists of the following components: Reagent trays 1 and 2 (RTT1 and RTT2) Reagent probes 1 and 2 (RPP1 and RPP2) Mixer 1 and 2 (MIX-1 and MIX-2) Barcode Reader Reagent Handling Area Learn about the reagent handling area. Slide NumberText BlocksCalloutsAudio ScriptImage File1 Reagent Positions   RTT1: 60 refrigerated positions Positions 1-56 are used for assay reagents Positions 57-60 are used for wash detergents that prevent contamintation   RTT2: 56 refrigerated positions Positions 1-52 are used for assay reagents Positions 53-56 are used for wash detergents  that prevent contamintation   Select Next to continue.   Note: If audio does not automatically start, select the play arrow in the top left to begin.Select each number to review the corresponding text.Callouts Reagent Tray 1 (RTT1) Reagent Tray 2 (RTT2) Reagent Tray Turntables 1 and 2 provide 116 refrigerated reagent holder positions. Several positions on each reagent turntable are used for ancillary reagents including pure water and wash detergents. Select Next to continue. 2 Reagent Probes Reagent probes (RPP1 and RPP2) aspirate the required reagent and dispense it into the RRV cuvettes for analysis. The Reagent probes are equipped with Liquid Level Sensing. These probes are washed with water over the Wash Port inside and outside after each pipetting cycle. Select Next to continue. The RPP1 and RPP2 aspirate the required reagents, and dispense those reagents, as dictated by the assay format into the RRV cuvettes. These reagent probes are equipped with Liquid Level Sensing and Liquid Surface Verification features in order to determine the amount of liquid present in the reagent containers. These probes are rinsed with water inside and outside over the Wash Port after each pipetting event. Select next to continue. 3 Mixers The mixers (MIX-1 and MIX-2) are located near their corresponding reagent probes. MIX-1 mixes samples with reagent 1 and MIX-2 mixes samples with reagent 2 when added.  Select Next to continue.  The reaction mixers, MIX 1 and MIX 2, stir the sample and reagent in the RRV cuvettes brought to their respective mixer positions. Strong and weak stirring options are available. Select next to continue. 4 Barcode Reader Barcode readers are located inside each Reagent Tray and the STT. The trays revolve and the barcode readers scan each reagent or sample position.   When complete, select the X in the upper-right corner to close the window and continue. Barcode readers are located inside each Reagent Tray and the STT. When a barcode scan is initiated, the Reagent Trays revolve and the barcode readers scan each reagent position. Then the system checks the level of liquid in the wedges in those positions. The system performs a barcode scan on the STT in order to locate and identify samples on that tray. Select the X in the upper right corner to close the window and continue.   Spectrophotometer and RRV Areas The spectrophotometer measures the amount of light absorbed at 14 specific wavelengths by the sample/reagent mixture contained in the reaction cuvettes. Spectrophotometer and RRV Learn about the spectrophotometer and RRV. Base ImageHotspotsText BlocksImage File The Spectrophotometer The spectrophotometer is located behind the lamp housing and is not visibly exposed. It measures the amount of light absorbed by the reaction mixture at 14 fixed wavelengths. The Halogen Lamp The lamp generates light which is absorbed by the reaction mixture. The light source is a 12 volt, 50 watt halogen lamp. Lamp Coolant Reservior A reservior holds the 5% lamp coolant which circulates to cool the halogen lamp. The Reaction Tray (RRV) The RRV is where sample and reagents are dispensed and the reaction takes place. The cuvettes are immersed in an oil bath at 37°C. The RRV rotates the cuvettes in front of the spectrophotometer to measure the reaction absorbance. It holds 340 resuable plastic cuvettes. The dead volume for each cuvette is 35uL. The minimum reaction volume is 80 uL. After analysis is complete the RRV cuvettes are washed by the WUD using water and wash solutions.   Key components located in the front of the analyzer include: Fluids and Pumps Emergency Stop Operate/Standby Buttons Key components located in the rear of the analyzer include: Main Power Switch Waste Line Key components of the Workstation include:  PC Keyboard Mouse Monitor   Front View Learn about the components located in the front of the analyzer. Base ImageHotspotsText BlocksImage File Ancillary Reagents The following liquids are located in the front area of the instrument: ISE Buffer 2 Reaction (RRV) bath oil Saline Cuvette Detergent Cuvette Conditioner Pure Water Bottle System Pumps The following pumps are located behind the solution tray: Sample Aspiration/Dispense Pump (SP) Dilution Probe Aspiration Pump (DIP) Dilution Probe Discharge Pump (DOP) Dilution Probe Wash Pump (DCP) Sample and Reagent Wash Pump (SRWP) Reagent Dispensing Pump 1 and Pump 2 (RP1 and RP2)  Operate/ Standby and Emergency buttons The operate/standby button changes the operating mode of the system. The emergency button immediately halts all operation of the system.   Personal Computer The Personal Computer powers the analyzer ON or OFF and contains a DVD/CD-RW drive that can store information.  Monitor The monitor allows you to view the analyzer software. Using your finger, the Touch-screen monitor allows you to perform your tasks directly at the monitor without using the keyboard.  Ancillary Reagents The following reagent is located on the left fluids door of the instrument: ISE Baseline Solution Rear Viiew Learn about the components located in the rear of the analyzer. Base ImageHotspotsText BlocksImage File The Waste Container This container is a receptacle for liquid waste from the analyzer.  According to your local regulations, you may need to separate the waste from the analyzer.  The Main Power Switch The Main Power switch allows you to execute a hard software and hardware reboot as needed.