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Sysmex® CA-1500 System Instrument Overview Online Training

Locate major system components and determine their function to provide the operator the foundation to successfully setup and operate the analyzer. Navigate through software screens for ease in system usability. This clinical laboratory training qualifies for continuing education units (CEU).

Select Next to continue. Describe the functions of system components Identify components of the Sysmex® CA-1500 System Identify key areas of the touch screen   Upon successful completion of this course, you will be able to: Congratulations. You have completed the Sysmex® CA-1500 System Instrument Overview Online Training course. In this online training course, you have learned how to: Identify key components of the Sysmex® CA-1500 System The Sysmex® CA-1500 System has three main areas: Main Instrument External Containers Printer Describe the functions of key components The sample probe aspirates sample from a tube or cup, dispenses the fluid into a sample plate well, aspirates sample fluid from the sample plate well, and then dispenses the fluid into a reaction tube. The catcher transports the reaction tube to an incubation well, transports the reaction tube with sample to the reagent probe for reagent dispensing and mixing, mixes the reagent and sample using vortex action, and then transports the reaction tube to an incubation well or coagulation, chromogenic, or immunologic detection well for test measurement. Finally, the catcher transfers the reaction tube to the tube trash box for disposal. Reagents are stored onboard the instrument in reagent trays that keep the reagents cooled to 15° C +2.0° C. Sample probe buffer and cleaning solutions are stored onboard the instrument at room temperature. Identify key areas of the Sysmex® CA-1500 System touch screen Sysmex® CA-1500 System software allows you to view coagulation curves, analysis results, error messages, and other system indicators from the touch screen monitor. Press touch screen keys to perform operations, navigate from screen to screen, or enter data. Select Next to continue.   The Sysmex® CA-1500 System can be divided into the following key areas: The Main Instrument handles sample processing, analysis, and reagent storage External Containers provide additional reagents to the system The Printer allows you to print out test reports, calibration curves, and QC information Select Next to continue.     The Sysmex® CA-1500 System performs clotting, chromogenic, and immunologic tests to measure coagulation factors. There are essential components that make up the Sysmex® CA-1500 System main instrument. These essential components can be located: On the Front of the instrument Left Side of the instrument Right Side of the instrument Internally Main Instrument Components Learn about the main instrument components. Slide NumberText BlocksCalloutsAudio ScriptImage File1 Main Instrument: Front    Components include: Sample transport system Light shield STAT sample cover and holder Touch screen monitor Reaction tube hopper   Select Next to continue. Note: If audio does not automatically start, select the play arrow in the top left to begin.The front of the main instrument includes the sample transport system-where samples move from the right input tray to the left output tray. At the front of the instrument you’ll also find the light shield, a STAT sample cover and holder, the touch screen monitor, and the reaction tube hopper, which holds reaction tubes. Select next to continue.2 Main Instrument: Left Side    Components include: Main power switch Fuses Power cord Tube trash drawer   Select Next to continue. The left side of the main instrument includes the main power switch and fuses, power cord, and tube trash drawer. Select next to continue.3 Main Instrument: Right Side    Components include: Disk drive for data storage Serial connection ports Pressure regulators Halogen source lamp Rinse and waste connections   Select Next to continue. The right side of the main instrument includes a disk drive for data storage, serial connection ports to external components, pressure regulators, halogen source lamp, and rinse and waste connections. Select next to continue.4 Main Instrument: Internal   Components include: Reagent trays Sampler plates Incubation and detector wells   Select Next to continue. Inside the main instrument you’ll find reagent trays, sampler plates, incubation wells, and detector wells. Select next to continue.5 Main Instrument: Catcher   Transports reaction tubes during sample processing and analysis.   Select Next to continue. Another important internal component is the catcher-the mechanism that transports reaction tubes from one station to the next during sample processing and analysis. Select next to continue.6Main Instrument: Other Internal Components include: Sample probe Reagent probe When complete, select the X in the upper-right corner to close the window and continue.  Other internal components include the sample and reagent probes. When complete, select the x in the upper-right corner to close the window and continue. Rinse Container: The Sysmex® CA-1500 System uses distilled water from the rinse container, to rinse both the reagent and sample probes, and to clean the hydraulic system. A float sensor inside the container determines when the container is empty and ready to be replaced. Note: Type 1 Reagent Grade water is recommended. Water in a cubitainer is recommended because the chance of contamination is less than if a separate container is used and continuously refilled. Waste Container: The waste container accumulates waste fluid, which is discarded when the container is almost full. A float sensor inside the container determines when the container is full and requires disposal. Tip: A full waste container is very heavy - empty the container before it is too heavy to lift. Select Next to continue.   The Sysmex® CA-1500 System is equipped with a color cartridge printer so you can print out: Patient test results QC data Coagulation curves Other statistics Select Next to continue. Sysmex® CA-1500 System software allows you to view coagulation curves, test results, error messages, and other system indicators from the touch screen monitor. Press touch screen keys to perform operations, navigate from screen to screen, or enter data. Touch Screen Learn about working with the touch screen monitor. Follow these tips when using the Sysmex® CA-1500 System touch screen: Use a pencil eraser to manipulate touch screen keys-sharp objects may damage the screen. The eraser can also improve accuracy if you wear protective gloves. To adjust the screen for better viewing, grasp the lower corners and push or pull the screen in or out. If there’s no screen activity for over five minutes, the LCD screen backlight will dim. Touch the screen anywhere to return to the original brightness. To clean the screen, use a very slightly water-dampened soft cloth. When complete, select the X in the upper-right corner to close the window and continue. The Main Menu screen comprises: System Status area displays indicators to alert you to the status of system operations. Data Processing area displays the rack number, sample ID, test ordered, and processing status of each sample displayed.  This area is reset if the instrument is powered down.  Press the Prev, Next, and Arrow keys to move from page to page.  Note: Analysis results do not appear on the Main Menu screen.  To view test results, press the Stored Data key in the Menu area. Menu- Press the menu option to navigate to a new screen where you can perform system functions, enter data, or navigate to another screen. The System Software Menu will show all menu options. Main Menu Screen Learn more about the Main Menu Screen. Base ImageHotspotsText BlocksImage FileReady: The analyzer status area displays the status of the analyzer (Ready, or Not Ready)Sysmex Key: The sysmex key provides a drop down list with four options: Screen Print: to print the current screen Error Log: to view most current and the last 21 hardware errors. The error log can hold up to 189 messages. If an error occurs the Sysmex key turns red and changes to 'Reset', and the error message appears to the right of the Sysmex key. To turn the alarm off, press 'Reset'. Auto QC: to enable automatic running of quality controls Quit: to return to previous screen GP or HC: The Peripheral Device Status area displays the peripheral devices the Sysmex CA-1500 System is physically attached to and are recognized by the software. For example, GP= graphic printer, HC= host computer. If these devices are actively communicating, they are backlit in green. If there is an error in communication, they are backlit in red.START: Press Start to start processing samples. Once selected, the Start key changes to Interrupt followed by Resume so that you can interrupt sample processing to replenish consumables or change orders without losing data.Lid: The Lid indicator light indicates if the light shield can be opened. If the light is green, the lid can be opened. If the light is red, the analyzer is processing samples and the lid should not be opened. If the lid is opened during operation, the analyzer will stop.STAT: Press the yellow STAT key to suspend processing of samples so that you can add a priority sample or remove a previously run STAT sample.Temperature: Temperatures for reagent cooling unit, reagent probe, clotting detector wells, immunologic/chromogenic detector wells, and room temperature appear here.Pressure: Pressure readings for pneumatics appear here. The pneumatics are on only when the analyzer is processing samples.Set Reagents: Press Set Reagents to verify reagent information as you load prepared reagents, calibrators, rinses, buffers, control plasmas, and factor deficient plasma into positions in reagent holders.Work List: Press Work List to manually enter, or register, sample ID, sample location, and test requests when you want to run patient samples, controls, or calibrators.Stored Data: Press Stored Data to view patient or QC data stored for the last 1,000 samples or 15,000 test results. When the data storage is full, the oldest result is overwritten by the newest result.QC: Press QC to open the Quality Control screen, where you can view QC data in a Levey-Jennings QC chart format.Standard Curve: Press Standard Curve to set up and review calibration curves and enter ISI information.Settings: Press Settings to view a list of options that allow you to customize the instrument to your lab's protocols.Rinse Probe: Press Rinse Probe to perform the daily maintenance task of washing the probes using CA Clean I.Special Menu: Press Special Menu to view two options: Press Maintain to perform maintenance tasks that are performed on an as needed basis, such as pressure adjustments and lamp calibration. Press System Test to perform hardware function tests, such as printing a test page on the printer.Data Processing Area: This area displays the rack number, sample ID, tests ordered, and processing status of each sample displayed. This area is reset if the instrument is powered down. Press the Prev, Next, and Arrow keys to move from page to page. Note: Analysis results do not appear on the Main Menu screen. To view results, press the Stored Data key in the Menu area. Key areas on the front of the Sysmex® CA-1500 System include: Light Shield Lid STAT Sample Cover Reaction Tube Hopper Touch Screen Sampler Mechanical Stop Switch Front Components Learn about the Front Components of the Sysmex® CA-1500 System. Base ImageHotspotsText BlocksImage FileTouch Screen: The Sysmex® CA-1500 System allows you to view coagulation curves, test results, error messages, and other system indicators from the liquid crystal display (LCD) touch screen monitor. Press the touch screen keys to perform operations, navigate from screen to screen, or enter data.Mechanical Stop Switch: Press the red emergency stop switch to stop ALL operation. Note: There are other ways to interrupt processing using the software when adding reagent or changing an order.Light Shield Lid: The light shield prevents ambient light from adversely affecting test results and helps maintain internal temperatures. If opened during operation, processing will stop.STAT Sample Cover: Push in the STAT sample cover to load a STAT sample into one of five positions of the STAT sample holder. You can order both uncapped sample tubes and sample cups as STATs.Sampler: The sampler automatically transports sample racks from the right input tray to the aspiration position (where the sample is aspirated), to the left output tray. The input tray can hold five racks at any one time. Sample Rack Full error will alert the operator when the output tray reaches 5 racks.Reaction Tube Hopper: The hopper holds a maximum of 300 disposable reaction tubes. Each test is performed in a single tube.STAT Sample Cover Close Key: Press the Close key to close the STAT sample cover. Sample Rack Learn about sample racks. Each sample rack can hold up to 10 samples-tubes or cups. Acceptable sample containers include: 3.2% or 3.8% sodium citrate tubes with dimensions of 10-15 mm (diameter) x 65 mm - 78 mm (length) 1.8-mL and 2.7-mL Vacutainer Plus Plastic Citrate Tube with HEMOGARD closure 2-mL or 4-mL sample cups When complete, select the X in the upper-right corner to close the window and continue. The left side of the Sysmex® CA-1500 System contains: Power Cord On/Off Switch Fuse Holder Cap Tube Trash Box Warning: When removing the tube trash box to discard used reaction tubes, be sure to follow biological hazard disposal procedures. Left Side Components Learn about the Left Side Components of the Sysmex® CA-1500 System. Base ImageHotspotsText BlocksImage FileTube Trash Box: The tube trash box can hold ~200 used reaction tubes. The instrument counts the number of used reaction tubes deposited into the trash box. A light reflective sensor indicates if the drawer has been removed.Fuse Holder Cap: The fuse holder cap contains two 10-AMP slow-blow fuses. On/Off Switch: 0= OFF, 1=ON Power Cord: The power cord supplies power to the instrument.   Key areas on the right side of the Sysmex® CA-1500 System include: Disk Drive LCD Brightness Control Knob Serial Connections: Includes four serial connection ports Wand Barcode Reader Connector Port HC (Host Computer) Connector Port GP (Graphic Printer) Connector Port DP (Data Printer)-not available in the USA Wand Barcode Reader Pressure Regulators Halogen Lamp Trap Chamber Sensor Connections Tube Connections Right Side Components Learn about components on the right side of the Sysmex® CA-1500 System. Base ImageHotspotsText BlocksImage FileLCD Brightness Control Knob: Turn the LCD knob to adjust the brightness of the touch screen.Disk Drive: You can output and input patient results, QC data and calibration curve data to a floppy disk. The instrument will accept 3.5-inch IBM-formatted 2HD disks.Wand Barcode Reader: Use the handheld barcode reader to scan barcode labels on reagents or STAT patient samples.HC (Host Computer) Connector Port: The HC connection allows data to be transmitted to and from the host computer and the Sysmex® CA-1500 System.Wand Barcode Reader Connector Port: The barcode connection connects an optional wand barcode reader.GP (Graphic Printer) Connection Port: The GP connection allows you to print test results or coagulation curves to the graphic printer supplied with the instrument.Pressure Regulators: The upper 1.0 Kg/cm2 knob regulates air pressure, and the lower 2.2 Kg/cm2 regulates master valve pressure.Halogen Lamp: The halogen lamp provides the light source for both chromogenic and immunologic test methods, such as AT3, Heparin, Plasminogen, and D-Dimer. The lamp is housed behind the external lamp cover.Trap Chamber: The trap chamber is used to prevent water from entering the pneumatic system and damaging the compressors.Sensor Connections: Float switch sensors trigger an alarm if the waste container is full or if the external rinse container needs to be refilled.Tube Connections: The rinse inlet draws distilled water into the system from the external rinse container. The waste outlet pumps waste into the waste container or to a floor or sink drain. Key internal components include: Reagent Tray Holder: Holds reagent trays 1 and 2. Reagent trays store reagents, calibrators, and control plasmas. You can use adaptors in each reagent tray to accommodate varying sizes of reagent vials and sample containers. The reagent tray holder's unique cooling unit stores reagents at 15oC + 2.0oC. Each tray has a drain for any condensation that may form. Detectors: The coagulation reaction occurs in wells located in detector blocks, which are heated and monitored to maintain 37oC +0.5oC. The coagulation reaction is detected through the change in scattered light (in clotting detector wells) or transmitted light (in chromogenic/immunologic detector wells). The detectors are located behind a metal shelf in back of the reagent trays to prevent light from entering the detection unit. Internal Components Learn about internal components. Base ImageHotspotsText BlocksImage FileChromogenic and Immunologic detector wells: The instrument provides 4 chromogenic/immunologic detector wells for measurement of both chromogenic and immunologic methods. The halogen lamp provides the light source for both these measurement types (Incubation well block to the right).Clotting detector and incubation wells: The instrument provides 14 incubation wells for sample incubation, and 8 clotting detector wells for clot detection. Each clotting detector well has its own light source- an LED at 660nm. (Incubation well block to the left).  Reagent Tray 1: Reagent tray 1 holds 22 reagents. Each reagent is assigned to a specific position in the tray. Reagent tray 1 positions include: A1, A2, B1-B10, and C1-C10.Reagent Tray 2: Reagent tray 2 holds 14 reagents in smaller size vials and sample cups, such as calibrators, factor deficient plasmas, and control plasmas. Each reagent is assigned to a specific position in the tray. Reagent tray 2 positions include D1-D14.Reagent Probe Wash Station: Reagent probe rinsing occurs at the reagent probe rinse station. Additional internal components of the Sysmex® CA-1500 System include: Sample Plates LED Sample Plates Room Temperature Reagents STAT Sample Holder Feeder Unit Sample Probe Wash Station Catcher More Internal Components Learn about additional internal components. Base ImageHotspotsText BlocksImage FileFeeder Unit: Reaction tubes travel from the hopper down the chute to the feeder unit. The feeder unit rotates to align reaction tubes to the catcher and from the sample probe to dispense sample into the reaction tube.Sample Plate LED: LED indicator lights show the status of the sample plate. Green= not used, Red= partially used, Flashing Red= completely used. If the LED is Off, the sensor has detected that the plate has been removed from the instrument.Sample Plates: A sample is aspirated and dispensed into a sample plate well. Each well has sufficient volume to perform all tests ordered for a sample-as well as auto-repeats, re-dilutions, and reflex testing. The instrument can hold up to five sample plates in position 1-5. 1 sample plate=50 wells.Room Temperature Reagent: Room temperature reagents and buffer are used by the sample probe. These reagents are stored in positions E1-E3. Position E3 is designated for CA Clean I, a detergent used for cleaning sample and reagent probes.STAT Sample Holder: The STAT sample holder can hold up to 5 STAT samples at a time.Sample Probe Wash Station: Sample probe rinsing occurs at the sample probe wash station.Catcher: The catcher transports reaction tubes during processing from the feeder unit, to the incubation well, to the detector well, and out to the trash box. The vortex mixing action of the catcher mixes reagents as they are dispensed by the reagent probe into reaction tubes. The Sysmex® CA-1500 System also includes: Sample Probe: The sample probe is located on the right hand side, inside the instrument. The sample probe can be either cap-piercing or non cap-piercing. Sample Probe Assembly: The sample probe assembly can consist of a cap-piercer or non cap-piercer sample probe. Reagent probe: The reagent probe is located on the left hand side, inside the instrument. The liquid sensing reagent probe aspirates reagents from reagent tray 1 and dispenses them into reaction tubes. Sample/Reagent Probe Learn about the sample and reagent probe. The liquid-sensing sample probe aspirates sample from a primary tube or cup and dispenses it into a sample plate well. The sample probe also aspirates samples from the sample plate wells into reaction tubes for processing. The sample probe performs re-dilutions, when necessary. In addition to handling patient samples, the sample probe also aspirates sample from calibrators, control plasmas, and factor deficient plasmas stored in reagent tray 2. The cap piercer sample probe sits in a white piercer guide on the cap piercer assembly. Reagent aspirated into the reagent probe is heated to 37oC. The reagent probe's curved tip prevents reagent foaming as contents are dispensed into reaction tubes.   When complete, select the X in the upper-right corner to close the window and continue. Welcome to the Sysmex® CA-1500 System Instrument Overview Online Training course. The Sysmex® CA-1500 System offers: Convenience Simplicity Flexibility Select Next to continue. The Sysmex® CA-1500 System performs clotting, chromogenic, and immunologic tests to measure coagulation factors. Depending on the analyte being measured, all three test methodologies can be performed simultaneously. The Sysmex® CA-1500 System also allows you to save valuable time by running simultaneous calibrations and samples. The instrument’s easy STAT access and simple maintenance offers flexibility and convenience. Other system features include: Auto-repeat, re-dilution and auto-reflex testing QC software package with a choice of control limit or westgard multirule QC evaluation methods Bi-directional host interface System Features Learn about system features. Term Definition Auto-repeat If repeat analysis limits have been set up, the instrument will automatically repeat the sample if a test yields a result that exceeds the test method's upper or lower repeat limits. (panic values established by each laboratory) Re-dilution If re-dilution analysis limits have been set up, the instrument will automatically re-dilute a sample if a test yields a result that exceeds the test method's upper or lower re-dilution limits. (The instrument either doubles or halves the instrument's normal dilution ratio, as applicable) Auto-reflex If reflex test conditions have been set up, the instrument will automatically run another test method for a sample if one or more tests ordered for that sample yield results outside the reflex limits set by the laboratory. You can set up to 60 types of reflex tests. Reflex testing improves the timeliness of results because the instrument automatically performs the next test needed without having to wait for the physician to see the initial result and ask for the next test. (For example, if PT <7 seconds, then run Fbg.) Control Limit QC Given the mean and standard deviation for a control material, control limits are calculated as the mean + 2 standard deviations (2s), or the mean + 3 standard deviations (3s) to determine if data should be accepted or rejected. Westgard Multirule QC Multirule QC uses a combination of decision criteria, or control rules, to decide if an analytical run is in control or out-of-control. The Westgard Multirule QC procedure uses six different control rules to judge the acceptability of an analytical run. Bi-directional host interface Two-way interface that allows you to download patient order information and transmit results to your host computer. (Laboratory Information System LIS) When Complete, select the X in the upper-right corner to close the window and continue. Westgard Multirule QC Learn more about westgard multirule. The Westgard Multirule QC check method uses six different control rules to judge the acceptability of an analytical run: 1-2s rule = A single QC result exceeded the mean ± 2SD (standard deviations). 1-3s rule = A single QC result exceeded the mean ± 3SD. 2-2s rule = Two consecutive QC results exceeded the mean ± 2SD. 4-1s rule = Four consecutive QC results exceeded the mean ± 1SD. R-4s rule = The difference between the current QC result and the previous QC result exceeded 4SD. 10x rule = Ten consecutive QC results fell on the same side of the mean. When complete, select the X in the upper-right corner to close the window and continue. Control Limit Learn more about control limit. The Control Limit check method evaluates if QC results exceed the mean ±2 standard deviations (2SD), or the mean ±3 standard deviations (3SD). If a QC result exceeds a control limit, the system flags the result in red on a Levey-Jennings QC chart, sounds an alarm, and records an error message on the error log as described below: Upper Stop Limit = mean +3SD. If a QC analysis result exceeds the upper stop limit, the instrument stops running, an alarm sounds, and the error log records a QC Exceeds Stop Limit error message. Upper Flag Limit = mean +2SD. If a QC analysis result exceeds the upper flag limit, an alarm sounds and the error log records a QC Flag Limit error message. Lower Flag Limit = mean -2SD. If a QC analysis result falls below the lower flag limit, an alarm sounds and the error log records a QC Flag Limit error message. Lower Stop Limit = mean -3SD. If a QC analysis result falls below the lower stop limit, the instrument stops running, an alarm sounds, and the error log records a QC Exceeds Stop Limit error message. When complete, select the X in the upper-right corner to close the window and continue.