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Sysmex® CA-1500 System Reagent Setup Online Training

Accurately identifying reagents and loading them properly on the Sysmex® CA-1500 System is a critical step in providing accurate patient results.

Welcome to the Sysmex® CA-1500 System Reagent Setup Online Training course. Reagent setup includes: Setting up reagent information using the Consumables screen (Type of reagent, assigned position, volume, lot number, expiration date, and vial size) Loading reagents into their assigned positions in the instrument Monitoring reagent volumes during operation Select Next to continue.   Upon successful completion of this course, you will be able to: Identify reagent positions in the Sysmex® CA-1500 System List reagent setup information Describe how to set up and load reagents into the instrument Select Next to continue. Identify key areas of the Consumables screen Congratulations. You have completed the Sysmex® CA-1500 System Reagent Setup online training course. Listed below are the key points that have been presented. Take time to review the material before you proceed to the final quiz. Identify reagent positions in the Sysmex® CA-1500 System instrument Reagent tray 1 holds up to 22 reagents at 15° C +2.0° C in positions A1 and A2 (for 50-mL vials of CA Clean I and II), B1 – B10 (for 5-mL vials), and C1 – C10 (for 15-mL vials). Reagent tray 2 holds up to 14 reagents at 15° C +2.0° C in positions D1- D14 (for 5-mL vials). Room temperature reagents are stored in positions E1 – E3 (for 50-mL vials of sample probe cleaner and buffer/diluent). Identify key areas of the Consumables screen Each reagent key on the Consumables screen corresponds to a reagent position in reagent tray 1, reagent tray 2, or the room temperature reagent positions. List reagent information that the instrument needs Before loading reagents, use the Consumables screen to set up reagents so that the instrument knows the type of reagent, position, volume, lot number, expiration date, and vial size of each reagent onboard the instrument. Demonstrate how to set up and load reagents into the instrument Using the Consumables screen, verify Group number, press a reagent key, and then scan reagent information into the system. Load the reagents into the position that corresponds to the same position on the Consumables screen. Select Next to continue. Designating specific reagent positions in the instrument allows the Sysmex® CA-1500 System software to keep track of reagent inventory. There are three instrument locations where you can designate reagent positions: Reagent tray 1 holds up to 22 reagents at 15° C ± 2.0° C. Designated positions in reagent tray 1 include: A1 and A2 (for 50-mL vials of CA Clean I and II). B1 - B10 (for 5-mL vials), and C1 - C10 (for 15-mL vials). Reagent tray 2 holds up to 14 reagents at 15° C ± 2.0° C. Designated positions in reagent tray 2 include: D1- D14 (for 5-mL vials). Room temperature reagents are stored in positions E1 - E3 (for 50-mL vials of sample probe cleaner and buffer/diluent). Note: Adapters may be used to accommodate smaller-size vial sizes (including 2- and 4-mL cups). CA Clean I and II Learn about CA Clean I and II. CA Clean I is a 1% Na hypochlorite solution. This detergent product is used for cleaning the sample and reagent probes on the Sysmex® CA-1500 System. CA Clean II is a 0.16% hydrochloric acid wash solution with a 0.5% nonionic surfactant. This detergent product is needed when the PT reagent (Dade® Innovin® reagent) is used on the Sysmex® CA-1500 System. When complete, select the X in the upper-right corner to close the window and continue. The Consumables screen shows the status of onboard consumables--including reagents and their positions. To view the Consumables screen: From the Main Menu screen, select Set Reagents. The Consumables screen will then appear. (Each reagent key corresponds to a reagent position in reagent tray 1, reagent tray 2, or the room temperature reagent positions. Other keys on the Consumables screen represent other consumables, such as reaction tubes and sample plates.) Keys that designate reagent positions display the test method name so that you know which reagent to load in that position. Keys are color-coded to indicate reagent volume. If multiple vials of the same reagent lot are loaded (maximum = three), reagent keys display one, two, or three symbols in the upper-left corner to indicate the order in which the instrument will use each vial. Test Groups Learn about test groups. You can set up two different test groups—Group 1 and Group 2. On the Consumables screen, press the Select Group button to toggle between Group 1 or Group 2. Group 1 can hold up to 15 test parameters. Group 2 is used if a lab is running more than 15 test parameters or if the lab wants to place a more esoteric test in Group 2. Groups are established during instrument setup using the Analysis Settings screen. Group parameters include test method, test position, reagent name, reagent lot number, reagent expiration, and reagent vial size. When complete, select the X in the upper-right corner to close the window and continue. Identify key areas of the consumable screen Learn more about key areas of the consumables screen. Base ImageHotspotsText BlocksImage FileA reagent positions hold 50-mL reagent vials.  You can set up two different test/reagent configurations--Group 1 and Group 2. The reagent configuration shown here is for Group 1 tests. Press the Select Group button to toggle between Group 1 or Group 2. There can be 15 assay parameters in Group 1, and 15 assay parameters in Group 2.  B reagent positions hold 5-mL reagent vials.  A blank key indicates that no test has been assigned to this position.  A red key indicates that the volume of reagent in this position has been depleted. This screen shows that Dade® Innovin® reagent in position C7 of reagent tray 1 is depleted.C reagent positions hold 15-mL reagent vials.D reagent positions hold 5-mL reagent vials.  A green key indicates that that the volume of reagent in this position is acceptable. This screen shows that 0.9 mL of SHP calibrator remains in position D14 in reagent tray 2.  During the sampling process, the sample probe aspirates sample from primary tubes or cups and dispenses it into a sample plate well. The Sysmex® CA-1500 System can hold up to five disposable sample plates in positions 1 – 5. Each sample plate contains 50 wells.  The Sysmex® CA-1500 System also uses room temperature reagents: sample probe cleaner and buffer/diluent. These reagents are stored in positions E1 – E3. E reagent positions hold room-temperature 50-mL reagent vials.  A yellow reagent vial key indicates that the volume of reagent in this position is getting low. A yellow sample plate key on the Consumables screen indicates the well is in use with some empty wells available. The number of empty wells available is displayed on the bottom of the key. This screen shows that there are 37 unused wells in sample plate position #1.  A green key indicates that the reaction tube supply is sufficient--the maximum number of tubes is 300--if the supply is low, the instrument alarm will sound.  Press the Select Group button to toggle between Group 1 or Group 2 test/reagent configurations. Use adapters to make varying-size containers fit into larger-size reagent positions. Adapters include: No. 93: For loading 5-mL vials into C positions No. 98: For loading 2-mL and 4-mL cups into D&B positions No. 97: For loading 2-mL and 4-mL cups into C positions No. 99: For loading 5-mL vials into A&E positions Select Next to continue. To enter reagent information, use the barcode wand to scan barcode labels on reagent vials—all Siemens Healthcare Diagnostics reagents have barcode labels. As an internal check, the check sum digit (an algorithm that checks that the numbers on the barcode label are read correctly) verifies that the barcode label was read correctly by the instrument. Note: Scanning the barcode label enters lot number and vial size. The system uses a default volume for each vial type or calculates it using level detection of the liquid surface. Expiration date is not included in the barcode data, so this parameter must be entered manually. Select Next to continue. After reviewing reagent inventory and preparing the required reagents, use the Consumables screen as a guide to load the reagents into their correct positions in the Sysmex® CA-1500 System instrument. To load reagents: On the Main Menu screen, press Set Reagents. On the Consumables screen, verify the Group number. To load existing reagents, press a reagent key, or to load new reagents, press an open key. Scan barcode labels on reagent vials. Verify reagent information: reagent volume, lot number, vial type, and expiration date. Load the reagent into the position that corresponds to the same position on the Consumables screen. Use an adapter, if necessary. Note: The Sysmex® CA-1500 System monitors reagent usage during operation mathematically by subtracting volumes used during sample processing or by the distance the probe descended before sensing liquid. Load Reagents Learn how to load reagents and update reagent information. Instructions:If media does not automatically start, select the play arrow to begin.Flash File:/content/generator/Course_90013591/sim_CA1500_ReagentSetup_LetsPractice_scaled/sim_CA1500_ReagentSetup_LetsPractice_scaled.htmHTML5 File:/content/generator/Course_90013591/sim_CA1500_ReagentSetup_LetsPractice_HTML5_800x600/index.htmlPDF File: